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Purification of ice structuring protein complexes from winter wheat using Triton X-114 phase partitioning
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作者 Huaneng XU Haiying CHEN Weining HUANG 《Frontiers of Chemical Science and Engineering》 SCIE EI CSCD 2009年第4期383-385,共3页
Ice structuring proteins(ISPs)isolated from the cold-acclimated plants have a great potential in improving the quality of frozen foods.The purification of ISP complexes from winter wheat was achieved using an aqueous ... Ice structuring proteins(ISPs)isolated from the cold-acclimated plants have a great potential in improving the quality of frozen foods.The purification of ISP complexes from winter wheat was achieved using an aqueous two-phase system of Triton X-114.The highly reactive phenols were removed,and the ISP complexes remained in the aqueous phase after phase separation.The ISP complexes treated by this procedure retained higher thermal hysteresis activity than those treated by ammonium sulfate method.The phase separation technique provides a simple and mild way for removing phenols from ISP complexes. 展开更多
关键词 ice structuring protein Triton X-114 PURIFICATION extraction PHENOLS
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