Idaho locust （Robinia pseudoacacia ‘Idaho＇） is an exotic multi-purpose tree used in landscaping, soil and water conserva- tion, fodder sources and others. To improve its drought tolerance for reclaiming arid land,...Idaho locust （Robinia pseudoacacia ‘Idaho＇） is an exotic multi-purpose tree used in landscaping, soil and water conserva- tion, fodder sources and others. To improve its drought tolerance for reclaiming arid land, five lines of transformed mtl-D gene, as osmotic regulator in plant cells, have been selected and managed to determine their drought tolerance under experimental conditions. Qualitative and quantitative variables of transformed plants were studied. The critical value of drought tolerance was determined by detecting the 2,3,5-triphenyl tetrazolium chloride （TTC） reductants in roots and soil water content （SWC）. The critical value for drought tolerance was SWC 6% while for the control plants the critical SWC was 8%; a moderate level of SWC is 13% and the highest SWC for plant endurance was 18%. The method proved to be reliable and sensitive in the evaluation of drought tolerance for forest trees.展开更多
Robinia pseudoacacia ‘Idaho＇ （Robinia x ambigua ‘Idahoensis＇, R. pseudoacacia x R. viscosa） modified by a mtLD gene went through five lines and had characteristics of drought tolerance. Three stages of their mic...Robinia pseudoacacia ‘Idaho＇ （Robinia x ambigua ‘Idahoensis＇, R. pseudoacacia x R. viscosa） modified by a mtLD gene went through five lines and had characteristics of drought tolerance. Three stages of their micropropagation had been studied by pre- vious investigators. The other two stages, in vitro shoot rooting and plantlet acclimatization, still remained unsolved in the laboratory. For this paper, we studied the later two stages based on the previous achievements. Results showed that the highest rooting rate of Idaho locust was 98.4% when the in vitro shoots, over 2.5 cm in height and 0.08 cm in diameter, were placed on a half strength MS basal medium with 0.4 mg.U1 IBA and 0.1 mg＇U1 NAA as supplements and were solidified with 0.5% agar; the highest survival rate was 98.3% when the rooted plantlets were potted in vermiculite. All the stages for micropropagation of the Idaho locust, modified by the mtl-D gene, were assembled completely. The tissue culture plants grow well in the field.展开更多
The transcription factor gene FpDREB2A of Fraxinus pennsylvanica Marsh var. subintegerrima （Vahl.） Fern was con- structed into the higher plant expression vector pBin438 and transformed into Robinia pseudoacacia ‘I...The transcription factor gene FpDREB2A of Fraxinus pennsylvanica Marsh var. subintegerrima （Vahl.） Fern was con- structed into the higher plant expression vector pBin438 and transformed into Robinia pseudoacacia ‘Idaho＇ by Agrobacterium tu- mefaciens GV3101. Callus was screened with G418. Morphogenesis of shoots and roots of Idaho locust transformed genes was car- ried out on antibiotic media. The transformed plants were verified by PCR and Southern blotting tests that the FpDREB2A gene had been inserted into the genome DNA of Idaho locust.展开更多
Robinia pseudoacacia ‘Idaho＇ is one of several multi-purpose trees used in ornamental, soil and water conservation, fodder and nectar sources. Plant abiotic stress tolerance transformed by genes could meet the requi...Robinia pseudoacacia ‘Idaho＇ is one of several multi-purpose trees used in ornamental, soil and water conservation, fodder and nectar sources. Plant abiotic stress tolerance transformed by genes could meet the requirements for reclamation of arid or alkalid lands and vegetation restoration. For this paper, we studied the effects of auxin and cytokine on Idaho locust in vitro regeneration and the establishment of gene transformation systems for plants mediated by Agrobacterium tumefaciens. Results showed that the ratios of cytokinin and auxin were the major factors affecting adventitious bud differentiation on a MS medium; the concentration of 0.5 mg·L^-16-BA benefitted callus proliferation and 0.25 mg·L^-1 IBA promoted shoot rooting; however, a higher IBA concentration will inhibit rooting. The most effective antitoxin for screening transgenic Idaho locust shoots was G418 and the most sensitive concentration of it was 8 mg·L^-1.展开更多
Based on the plant regeneration system, a GUS gene transformation system to Idaho locust （Robinia pseudoacacia ‘Idaho＇） mediated by Agrobacterium tumefaciens was established. The successful transformation was conf...Based on the plant regeneration system, a GUS gene transformation system to Idaho locust （Robinia pseudoacacia ‘Idaho＇） mediated by Agrobacterium tumefaciens was established. The successful transformation was confirmed by regenerating the shoots fi＇om the infected leaves in the presence of hygromysin; by histochemical X-gluc assays of 15-glucuronidase （GUS） and by PCR and PCR-Southern blotting analysis. The ratio of positive transgenic plants is 5.8% （5 out of 86 plants）. With this system, the target gene DREB was introduced into the leaves of Idaho locust. The transgenic plants regenerated, which was verified by PCR-Southern blot- ting. It is suggested that the transformation system could be a new, simple, reliable and practical route to gene transformation of R. pseudoacacia ＇Idaho＇ mediated with A. tumefaciens.展开更多
岗位职业分析法(Developing A Curriculum,DACUM)在美国课程开发和运用过程中已经非常普遍和成熟,是美国以"能力为本"的职业教育与培训课程建构的重要保障。以爱达荷路易斯州立学院承接的企业初级计算机辅助制图和设计(Comput...岗位职业分析法(Developing A Curriculum,DACUM)在美国课程开发和运用过程中已经非常普遍和成熟,是美国以"能力为本"的职业教育与培训课程建构的重要保障。以爱达荷路易斯州立学院承接的企业初级计算机辅助制图和设计(Computer Aided Drafting and Designing,CADD)培训项目为例,对DACUM方法在培训课程体系建构中的运用、调整及其重要性进行介绍,以期为我国职业院校承担社会培训教育提供有益的借鉴。展开更多
We developed a habitat suitability model for predicting nest locations of breeding Northern Goshawks (Accipiter gentilis) in the high-elevation mixed forest and shrub-steppe habitat of south-central Idaho, USA. We use...We developed a habitat suitability model for predicting nest locations of breeding Northern Goshawks (Accipiter gentilis) in the high-elevation mixed forest and shrub-steppe habitat of south-central Idaho, USA. We used elevation, slope, aspect, ruggedness, distance-to-water, canopy cover, and individual bands of Landsat imagery as predictors for known nest locations with logistic regression. We found goshawks prefer to nest in gently-sloping, east-facing, non-rugged areas of dense aspen and lodgepole pine forests with low reflectance in green (0.53 - 0.61 μm) wavelengths during the breeding season. We used the model results to classify our 43,169 hectare study area into nesting suitability categories: well suited (8.8%), marginally suited (5.1%), and poorly suited (86.1%). We evaluated our model’s performance by comparing the modeled results to a set of GPS locations of known nests (n = 15) that were not used to develop the model. Observed nest locations matched model results 93.3% of the time for well suited habitat and fell within poorly suited areas only 6.7% of the time. Our method improves on goshawk nesting models developed previously by others and may be applicable for surveying goshawks in adjacent mountain ranges across the northern Great Basin.展开更多
文摘Idaho locust （Robinia pseudoacacia ‘Idaho＇） is an exotic multi-purpose tree used in landscaping, soil and water conserva- tion, fodder sources and others. To improve its drought tolerance for reclaiming arid land, five lines of transformed mtl-D gene, as osmotic regulator in plant cells, have been selected and managed to determine their drought tolerance under experimental conditions. Qualitative and quantitative variables of transformed plants were studied. The critical value of drought tolerance was determined by detecting the 2,3,5-triphenyl tetrazolium chloride （TTC） reductants in roots and soil water content （SWC）. The critical value for drought tolerance was SWC 6% while for the control plants the critical SWC was 8%; a moderate level of SWC is 13% and the highest SWC for plant endurance was 18%. The method proved to be reliable and sensitive in the evaluation of drought tolerance for forest trees.
文摘Robinia pseudoacacia ‘Idaho＇ （Robinia x ambigua ‘Idahoensis＇, R. pseudoacacia x R. viscosa） modified by a mtLD gene went through five lines and had characteristics of drought tolerance. Three stages of their micropropagation had been studied by pre- vious investigators. The other two stages, in vitro shoot rooting and plantlet acclimatization, still remained unsolved in the laboratory. For this paper, we studied the later two stages based on the previous achievements. Results showed that the highest rooting rate of Idaho locust was 98.4% when the in vitro shoots, over 2.5 cm in height and 0.08 cm in diameter, were placed on a half strength MS basal medium with 0.4 mg.U1 IBA and 0.1 mg＇U1 NAA as supplements and were solidified with 0.5% agar; the highest survival rate was 98.3% when the rooted plantlets were potted in vermiculite. All the stages for micropropagation of the Idaho locust, modified by the mtl-D gene, were assembled completely. The tissue culture plants grow well in the field.
文摘The transcription factor gene FpDREB2A of Fraxinus pennsylvanica Marsh var. subintegerrima （Vahl.） Fern was con- structed into the higher plant expression vector pBin438 and transformed into Robinia pseudoacacia ‘Idaho＇ by Agrobacterium tu- mefaciens GV3101. Callus was screened with G418. Morphogenesis of shoots and roots of Idaho locust transformed genes was car- ried out on antibiotic media. The transformed plants were verified by PCR and Southern blotting tests that the FpDREB2A gene had been inserted into the genome DNA of Idaho locust.
文摘Robinia pseudoacacia ‘Idaho＇ is one of several multi-purpose trees used in ornamental, soil and water conservation, fodder and nectar sources. Plant abiotic stress tolerance transformed by genes could meet the requirements for reclamation of arid or alkalid lands and vegetation restoration. For this paper, we studied the effects of auxin and cytokine on Idaho locust in vitro regeneration and the establishment of gene transformation systems for plants mediated by Agrobacterium tumefaciens. Results showed that the ratios of cytokinin and auxin were the major factors affecting adventitious bud differentiation on a MS medium; the concentration of 0.5 mg·L^-16-BA benefitted callus proliferation and 0.25 mg·L^-1 IBA promoted shoot rooting; however, a higher IBA concentration will inhibit rooting. The most effective antitoxin for screening transgenic Idaho locust shoots was G418 and the most sensitive concentration of it was 8 mg·L^-1.
文摘Based on the plant regeneration system, a GUS gene transformation system to Idaho locust （Robinia pseudoacacia ‘Idaho＇） mediated by Agrobacterium tumefaciens was established. The successful transformation was confirmed by regenerating the shoots fi＇om the infected leaves in the presence of hygromysin; by histochemical X-gluc assays of 15-glucuronidase （GUS） and by PCR and PCR-Southern blotting analysis. The ratio of positive transgenic plants is 5.8% （5 out of 86 plants）. With this system, the target gene DREB was introduced into the leaves of Idaho locust. The transgenic plants regenerated, which was verified by PCR-Southern blot- ting. It is suggested that the transformation system could be a new, simple, reliable and practical route to gene transformation of R. pseudoacacia ＇Idaho＇ mediated with A. tumefaciens.
文摘岗位职业分析法(Developing A Curriculum,DACUM)在美国课程开发和运用过程中已经非常普遍和成熟,是美国以"能力为本"的职业教育与培训课程建构的重要保障。以爱达荷路易斯州立学院承接的企业初级计算机辅助制图和设计(Computer Aided Drafting and Designing,CADD)培训项目为例,对DACUM方法在培训课程体系建构中的运用、调整及其重要性进行介绍,以期为我国职业院校承担社会培训教育提供有益的借鉴。
文摘We developed a habitat suitability model for predicting nest locations of breeding Northern Goshawks (Accipiter gentilis) in the high-elevation mixed forest and shrub-steppe habitat of south-central Idaho, USA. We used elevation, slope, aspect, ruggedness, distance-to-water, canopy cover, and individual bands of Landsat imagery as predictors for known nest locations with logistic regression. We found goshawks prefer to nest in gently-sloping, east-facing, non-rugged areas of dense aspen and lodgepole pine forests with low reflectance in green (0.53 - 0.61 μm) wavelengths during the breeding season. We used the model results to classify our 43,169 hectare study area into nesting suitability categories: well suited (8.8%), marginally suited (5.1%), and poorly suited (86.1%). We evaluated our model’s performance by comparing the modeled results to a set of GPS locations of known nests (n = 15) that were not used to develop the model. Observed nest locations matched model results 93.3% of the time for well suited habitat and fell within poorly suited areas only 6.7% of the time. Our method improves on goshawk nesting models developed previously by others and may be applicable for surveying goshawks in adjacent mountain ranges across the northern Great Basin.
