Background:Aspergillus fumigatus(Af)is one of the most ubiquitous fungi and its infection potency is suggested to be strongly controlled by the host genetic back-ground.The aim of this study was to search for candidat...Background:Aspergillus fumigatus(Af)is one of the most ubiquitous fungi and its infection potency is suggested to be strongly controlled by the host genetic back-ground.The aim of this study was to search for candidate genes associated with host susceptibility to Aspergillus fumigatus(Af)using an RNAseq approach in CC lines and hepatic gene expression.Methods:We studied 31 male mice from 25 CC lines at 8 weeks old;the mice were infected with Af.Liver tissues were extracted from these mice 5 days post-infection,and next-generation RNA-sequencing(RNAseq)was performed.The GENE-E analysis platform was used to generate a clustered heat map matrix.Results:Significant variation in body weight changes between CC lines was ob-served.Hepatic gene expression revealed 12 top prioritized candidate genes differ-entially expressed in resistant versus susceptible mice based on body weight changes.Interestingly,three candidate genes are located within genomic intervals of the previ-ously mapped quantitative trait loci(QTL),including Gm16270 and Stox1 on chromo-some 10 and Gm11033 on chromosome 8.Conclusions:Our findings emphasize the CC mouse model's power in fine mapping the genetic components underlying susceptibility towards Af.As a next step,eQTL analysis will be performed for our RNA-Seq data.Suggested candidate genes from our study will be further assessed with a human cohort with aspergillosis.展开更多
Oviductus Ranae is the dried oviduct of female Rana tem-poraria chensinensis （David）, distributed mainly in North- eastern China. Oviductus Ranae is one of the best-known and highly valued oriental foods and medicin...Oviductus Ranae is the dried oviduct of female Rana tem-poraria chensinensis （David）, distributed mainly in North- eastern China. Oviductus Ranae is one of the best-known and highly valued oriental foods and medicines. Traditional Chinese medicine holds that Oviductus Ranae can nourish yin, moisten lung and replenish the kidney essence. Meanwhile, activities of Oviductus Ranae such as anti-aging, anti-lipemic, anti-oxidation and anti-fatigue have also been demonstrated by modern phar-macological studies. Previous studies have shown that Oviductus Ranae is mainly composed of proteins, which are up to 50% or more.展开更多
The soft-shelled turtle Pelodiscus sinensis is a high-profile turtle species because of its nutritional and medicinal value in Asian countries.However,little is known about the genes that are involved in formation of ...The soft-shelled turtle Pelodiscus sinensis is a high-profile turtle species because of its nutritional and medicinal value in Asian countries.However,little is known about the genes that are involved in formation of their nutritional quality traits,especially the molecular mechanisms responsible for unsaturated fatty acid and collagen biosynthesis.In the present study,the transcriptomes from six tissues from Pelodiscus sinensis were sequenced using an Illumina paired-end sequencing platform.We obtained more than 47 million sequencing reads and 73 954 unigenes with an average size of 754 bp by de-novo assembly.In total,55.19% of the unigenes(40 814) had significant similarity with proteins in the National Center of Biotechnology Information(NCBI) non-redundant protein database and Swiss-Prot database(E-value <10 5).Of these annotated unigenes,9 156 and 11 947 unigenes were assigned to 52 gene ontology categories(GO) and 25 clusters of orthologous groups(COG),respectively.In total,26 496(35.83%) unigenes were assigned to 242 pathways using the Kyoto Encyclopedia of Genes and Genomes pathway database(KEGG).In addition,we found a number of highly expressed genes involved in the regulation of P.sinensis unsaturated fatty acid biosynthesis and collagen formation,including desaturases,growth factors,transcription factors,and extracellular matrix components.Our data represent the most comprehensive sequence resource available for the Chinese soft-shelled turtle and could provide a basis for new research on this turtle as well as the molecular genetics and functional genomics of other terrapins.To our knowledge,we report for the first time,the large-scale RNA sequencing(RNA-Seq) of terrapin animals and would enrich the knowledge of turtles for future research.展开更多
基金European Sequencing and Genotyping Institutes(ESGI),Grant/Award Number:075491/Z/04,085906/Z/08/Z and 090532/Z/09/ZTel-Aviv University(TAU)。
文摘Background:Aspergillus fumigatus(Af)is one of the most ubiquitous fungi and its infection potency is suggested to be strongly controlled by the host genetic back-ground.The aim of this study was to search for candidate genes associated with host susceptibility to Aspergillus fumigatus(Af)using an RNAseq approach in CC lines and hepatic gene expression.Methods:We studied 31 male mice from 25 CC lines at 8 weeks old;the mice were infected with Af.Liver tissues were extracted from these mice 5 days post-infection,and next-generation RNA-sequencing(RNAseq)was performed.The GENE-E analysis platform was used to generate a clustered heat map matrix.Results:Significant variation in body weight changes between CC lines was ob-served.Hepatic gene expression revealed 12 top prioritized candidate genes differ-entially expressed in resistant versus susceptible mice based on body weight changes.Interestingly,three candidate genes are located within genomic intervals of the previ-ously mapped quantitative trait loci(QTL),including Gm16270 and Stox1 on chromo-some 10 and Gm11033 on chromosome 8.Conclusions:Our findings emphasize the CC mouse model's power in fine mapping the genetic components underlying susceptibility towards Af.As a next step,eQTL analysis will be performed for our RNA-Seq data.Suggested candidate genes from our study will be further assessed with a human cohort with aspergillosis.
基金supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (No. 2011BAI03B00)the National Science and Technology Major Project of the Ministry of Science and Technology of China (No. 2011ZX09401-305)
文摘Oviductus Ranae is the dried oviduct of female Rana tem-poraria chensinensis （David）, distributed mainly in North- eastern China. Oviductus Ranae is one of the best-known and highly valued oriental foods and medicines. Traditional Chinese medicine holds that Oviductus Ranae can nourish yin, moisten lung and replenish the kidney essence. Meanwhile, activities of Oviductus Ranae such as anti-aging, anti-lipemic, anti-oxidation and anti-fatigue have also been demonstrated by modern phar-macological studies. Previous studies have shown that Oviductus Ranae is mainly composed of proteins, which are up to 50% or more.
基金Project supported by the National Basic Research Program (973) of China (No.2011CB111500)the Natural Science Foundation of Ningbo City,China (Nos.2011A610012 and 2011A610017)+1 种基金the Zhejiang Provincial Project of Selective Breeding of Aquatic New Varieties,China (No.2012C12907)the Zhejiang Provincial Top Key Discipline of Modern Microbiology and Application,China (Nos.KF2010005 and KF2012003)
文摘The soft-shelled turtle Pelodiscus sinensis is a high-profile turtle species because of its nutritional and medicinal value in Asian countries.However,little is known about the genes that are involved in formation of their nutritional quality traits,especially the molecular mechanisms responsible for unsaturated fatty acid and collagen biosynthesis.In the present study,the transcriptomes from six tissues from Pelodiscus sinensis were sequenced using an Illumina paired-end sequencing platform.We obtained more than 47 million sequencing reads and 73 954 unigenes with an average size of 754 bp by de-novo assembly.In total,55.19% of the unigenes(40 814) had significant similarity with proteins in the National Center of Biotechnology Information(NCBI) non-redundant protein database and Swiss-Prot database(E-value <10 5).Of these annotated unigenes,9 156 and 11 947 unigenes were assigned to 52 gene ontology categories(GO) and 25 clusters of orthologous groups(COG),respectively.In total,26 496(35.83%) unigenes were assigned to 242 pathways using the Kyoto Encyclopedia of Genes and Genomes pathway database(KEGG).In addition,we found a number of highly expressed genes involved in the regulation of P.sinensis unsaturated fatty acid biosynthesis and collagen formation,including desaturases,growth factors,transcription factors,and extracellular matrix components.Our data represent the most comprehensive sequence resource available for the Chinese soft-shelled turtle and could provide a basis for new research on this turtle as well as the molecular genetics and functional genomics of other terrapins.To our knowledge,we report for the first time,the large-scale RNA sequencing(RNA-Seq) of terrapin animals and would enrich the knowledge of turtles for future research.
