Assessment and Visualization of Ki67 Heterogeneity in Breast Cancers through Digital Image Analysis

The Ki67 index (KI) is a standard clinical marker for tumor proliferation;however, its application is hindered by intratumoral heterogeneity. In this study, we used digital image analysis to comprehensively analyze Ki67 heterogeneity and distribution patterns in breast carcinoma. Using Smart Pathology software, we digitized and analyzed 42 excised breast carcinoma Ki67 slides. Boxplots, histograms, and heat maps were generated to illustrate the KI distribution. We found that 30% of cases (13/42) exhibited discrepancies between global and hotspot KI when using a 14% KI threshold for classification. Patients with higher global or hotspot KI values displayed greater heterogenicity. Ki67 distribution patterns were categorized as randomly distributed (52%, 22/42), peripheral (43%, 18/42), and centered (5%, 2/42). Our sampling simulator indicated analyzing more than 10 high-power fields was typically required to accurately estimate global KI, with sampling size being correlated with heterogeneity. In conclusion, using digital image analysis in whole-slide images allows for comprehensive Ki67 profile assessment, shedding light on heterogeneity and distribution patterns. This spatial information can facilitate KI surveys of breast cancer and other malignancies.

Sentiment Analysis Using E-Commerce Review Keyword-Generated Image with a Hybrid Machine Learning-Based Model

In the context of the accelerated pace of daily life and the development of e-commerce,online shopping is a mainstreamway for consumers to access products and services.To understand their emotional expressions in facing different shopping experience scenarios,this paper presents a sentiment analysis method that combines the ecommerce reviewkeyword-generated imagewith a hybrid machine learning-basedmodel,inwhich theWord2Vec-TextRank is used to extract keywords that act as the inputs for generating the related images by generative Artificial Intelligence(AI).Subsequently,a hybrid Convolutional Neural Network and Support Vector Machine(CNNSVM)model is applied for sentiment classification of those keyword-generated images.For method validation,the data randomly comprised of 5000 reviews from Amazon have been analyzed.With superior keyword extraction capability,the proposedmethod achieves impressive results on sentiment classification with a remarkable accuracy of up to 97.13%.Such performance demonstrates its advantages by using the text-to-image approach,providing a unique perspective for sentiment analysis in the e-commerce review data compared to the existing works.Thus,the proposed method enhances the reliability and insights of customer feedback surveys,which would also establish a novel direction in similar cases,such as social media monitoring and market trend research.

Automatic area estimation of algal blooms in water bodies from UAV images using texture analysis

Algal blooms,the spread of algae on the surface of water bodies,have adverse effects not only on aquatic ecosystems but also on human life.The adverse effects of harmful algal blooms(HABs)necessitate a convenient solution for detection and monitoring.Unmanned aerial vehicles(UAVs)have recently emerged as a tool for algal bloom detection,efficiently providing on-demand images at high spatiotemporal resolutions.This study developed an image processing method for algal bloom area estimation from the aerial images(obtained from the internet)captured using UAVs.As a remote sensing method of HAB detection,analysis,and monitoring,a combination of histogram and texture analyses was used to efficiently estimate the area of HABs.Statistical features like entropy(using the Kullback-Leibler method)were emphasized with the aid of a gray-level co-occurrence matrix.The results showed that the orthogonal images demonstrated fewer errors,and the morphological filter best detected algal blooms in real time,with a precision of 80%.This study provided efficient image processing approaches using on-board UAVs for HAB monitoring.

Tongue image feature correlation analysis in benign lung nodules and lung cancer

Objective To analyze the differences in the correlation of tongue image indicators among patients with benign lung nodules and lung cancer.Methods From July 1;2020 to March 31;2022;clinical information of lung cancer patients and benign lung nodules patients was collected at the Oncology Department of Longhua Hos-pital Affiliated to Shanghai University of Traditional Chinese Medicine and the Physical Ex-amination Center of Shuguang Hospital Affiliated to Shanghai University of Traditional Chi-nese Medicine;respectively.We obtained tongue images from patients with benign lung nod-ules and lung cancer using the TFDA-1 digital tongue diagnosis instrument;and analyzed these images with the TDAS V2.0 software.The extracted indicators included color space pa-rameters in the Lab system for both the tongue body(TB)and tongue coating(TC)(TB/TC-L;TB/TC-a;and TB/TC-b);textural parameters[TB/TC-contrast(CON);TB/TC-angular second moment(ASM);TB/TC-entropy(ENT);and TB/TC-MEAN];as well as TC parameters(perAll and perPart).The bivariate correlation of TB and TC features was analyzed using Pearson’s or Spearman’s correlation analysis;and the overall correlation was analyzed using canonical correlation analysis(CCA).Results Samples from 307 patients with benign lung nodules and 276 lung cancer patients were included after excluding outliers and extreme values.Simple correlation analysis indi-cated that the correlation of TB-L with TC-L;TB-b with TC-b;and TB-b with perAll in lung cancer group was higher than that in benign nodules group.Moreover;the correlation of TB-a with TC-a;TB-a with perAll;and the texture parameters of the TB(TB-CON;TB-ASM;TB-ENT;and TB-MEAN)with the texture parameters of the TC(TC-CON;TC-ASM;TC-ENT;and TC-MEAN)in benign nodules group was higher than lung cancer group.CCA further demon-strated a strong correlation between the TB and TC parameters in lung cancer group;with the first and second pairs of typical variables in benign nodules and lung cancer groups indicat-ing correlation coefficients of 0.918 and 0.817(P<0.05);and 0.940 and 0.822(P<0.05);re-spectively.Conclusion Benign lung nodules and lung cancer patients exhibited differences in correla-tion in the L;a;and b values of the TB and TC;as well as the perAll value of the TC;and the texture parameters(TB/TC-CON;TB/TC-ASM;TB/TC-ENT;and TB/TC-MEAN)between the TB and TC.Additionally;there were differences in the overall correlation of the TB and TC be-tween the two groups.Objective tongue diagnosis indicators can effectively assist in the diag-nosis of benign lung nodules and lung cancer;thereby providing a scientific basis for the ear-ly detection;diagnosis;and treatment of lung cancer.

Automated Extraction and Analysis of CBC Test from Scanned Images

Health care is an important part of human life and is a right for everyone. One of the most basic human rights is to receive health care whenever they need it. However, this is simply not an option for everyone due to the social conditions in which some communities live and not everyone has access to it. This paper aims to serve as a reference point and guide for users who are interested in monitoring their health, particularly their blood analysis to be aware of their health condition in an easy way. This study introduces an algorithmic approach for extracting and analyzing Complete Blood Count (CBC) parameters from scanned images. The algorithm employs Optical Character Recognition (OCR) technology to process images containing tabular data, specifically targeting CBC parameter tables. Upon image processing, the algorithm extracts data and identifies CBC parameters and their corresponding values. It evaluates the status (High, Low, or Normal) of each parameter and subsequently presents evaluations, and any potential diagnoses. The primary objective is to automate the extraction and evaluation of CBC parameters, aiding healthcare professionals in swiftly assessing blood analysis results. The algorithmic framework aims to streamline the interpretation of CBC tests, potentially improving efficiency and accuracy in clinical diagnostics.

Optimal Classification of Minerals by Microscopic Image Analysis Based on Seven-State “Deep Learning” Combined with Optimizers

The development of artificial intelligence (AI), particularly deep learning, has made it possible to accelerate and improve the processing of data collected in different fields (commerce, medicine, surveillance or security, agriculture, etc.). Most related works use open source consistent image databases. This is the case for ImageNet reference data such as coco data, IP102, CIFAR-10, STL-10 and many others with variability representatives. The consistency of its images contributes to the spectacular results observed in its fields with deep learning. The application of deep learning which is making its debut in geology does not, to our knowledge, include a database of microscopic images of thin sections of open source rock minerals. In this paper, we evaluate three optimizers under the AlexNet architecture to check whether our acquired mineral images have object features or patterns that are clear and distinct to be extracted by a neural network. These are thin sections of magmatic rocks (biotite and 2-mica granite, granodiorite, simple granite, dolerite, charnokite and gabbros, etc.) which served as support. We use two hyper-parameters: the number of epochs to perform complete rounds on the entire data set and the “learning rate” to indicate how quickly the weights in the network will be modified during optimization. Using Transfer Learning, the three (3) optimizers all based on the gradient descent methods of Stochastic Momentum Gradient Descent (sgdm), Root Mean Square Propagation (RMSprop) algorithm and Adaptive Estimation of moment (Adam) achieved better performance. The recorded results indicate that the Momentum optimizer achieved the best scores respectively of 96.2% with a learning step set to 10−3 for a fixed choice of 350 epochs during this variation and 96, 7% over 300 epochs for the same value of the learning step. This performance is expected to provide excellent insight into image quality for future studies. Then they participate in the development of an intelligent system for the identification and classification of minerals, seven (7) in total (quartz, biotite, amphibole, plagioclase, feldspar, muscovite, pyroxene) and rocks.

From text to image:challenges in integrating vision into ChatGPT for medical image interpretation

Large language models(LLMs),such as ChatGPT developed by OpenAI,represent a significant advancement in artificial intelligence(AI),designed to understand,generate,and interpret human language by analyzing extensive text data.Their potential integration into clinical settings offers a promising avenue that could transform clinical diagnosis and decision-making processes in the future(Thirunavukarasu et al.,2023).This article aims to provide an in-depth analysis of LLMs’current and potential impact on clinical practices.Their ability to generate differential diagnosis lists underscores their potential as invaluable tools in medical practice and education(Hirosawa et al.,2023;Koga et al.,2023).

Radiomics and molecular analysis:Bridging the gap for predicting hepatocellular carcinoma prognosis

This editorial examines a recent study that used radiomics based on computed tomography(CT)to predict the expression of the fibroblast-related gene enhancer of zeste homolog 2(EZH2)and its correlation with the survival of patients with hepatocellular carcinoma(HCC).By integrating radiomics with molecular analysis,the study presented a strategy for accurately predicting the expression of EZH2 from CT scans.The findings demonstrated a strong link between the radiomics model,EZH2 expression,and patient prognosis.This noninvasive approach provides valuable insights into the therapeutic management of HCC.

Automated Registration for Infrared Image Based on Wavelet Analysis

To develop a quick, accurate and antinoise automated image registration technique for infrared images, the wavelet analysis technique was used to extract the feature points in two images followed by the compensation for input image with angle difference between them. A hi erarchical feature matching algorithm was adopted to get the final transform parameters between the two images. The simulation results for two infrared images show that the method can effectively, quickly and accurately register images and be antinoise to some extent.

3D characterization and analysis of pore structure of packed ore particle beds based on computed tomography images

Methods and procedures of three-dimensional （3D） characterization of the pore structure features in the packed ore particle bed are focused. X-ray computed tomography was applied to deriving the cross-sectional images of specimens with single particle size of 1-2, 2-3, 3-4, 4-5, 5-6, 6-7, 7-8, 8-9, 9-10 ram. Based on the in-house developed 3D image analysis programs using Matlab, the volume porosity, pore size distribution and degree of connectivity were calculated and analyzed in detail. The results indicate that the volume porosity, the mean diameter of pores and the effective pore size （d50） increase with the increasing of particle size. Lognormal distribution or Gauss distribution is mostly suitable to model the pore size distribution. The degree of connectivity investigated on the basis of cluster-labeling algorithm also increases with increasing the particle size approximately.

Fish maneuver analysis system based on sequence images

The aim is to establish an automatic system to analyze the maneuver performance of fish. A high speed camera （1 000 frame/s） is employed to record fast-start maneuver. Three steps are taken to analyze the kinematics： first, the midline in the first image is partitioned into equal interval lengths and the coordinates of all inter segmental points are saved. Secondly, these points coordinates are searched in the next frame with the digital image correlation （DIC） method, then these points are fitted with a spline curve function. Repeat this step until all the midlines are figured out frame by frame. Finally, according to the variety of midlines, the kinematics of the fast-start is calculated. Using this system to test carp C-start, the duration is divided into two stages： stage 1 is defined as the formation of the C shape and stage 2 as the return flip of the tail followed with forward motion. By tracing the middle line, the kinematic parameters of turning rate, centre of mass （CM） turning rate, CM turning radius, etc. are obtained.

Fluorescence-assisted Image Analysis of Harmful Microalgae

In order to realize the detection and analysis of microalgae in sediment samples with complex scenes, the project takes advantage of the character of microalgae that they can auto-fluoresce when exposed to the illumination of certain exciation waves. The project takes grey-scale and fluorescent pictures of microalgae in the same field of view and uses the image processing technique to deal with the images, such as threshold segmentation, contour and texture analysis and pattern recognition. The results show that the fluorescent image can effectively elimate the yawp in the complex background and make the consequent image processing more effective and easy. Then the project comes to the conclusion that fluorescence-assisted image processing can realize the detection and analysis of microalgae in sediment samples containing complex scenes.

EFFECTIVE FEATURE ANALYSIS FOR COLOR IMAGE SEGMENTATION

An approach for color image segmentation is proposed based on the contributions of color features to segmentation rather than the choice of a particular color space. The determination of effective color features depends on the analysis of various color features from each tested color image via the designed feature encoding. It is different from the pervious methods where self organized feature map (SOFM) is used for constructing the feature encoding so that the feature encoding can self organize the effective features for different color images. Fuzzy clustering is applied for the final segmentation when the well suited color features and the initial parameter are available. The proposed method has been applied in segmenting different types of color images and the experimental results show that it outperforms the classical clustering method. The study shows that the feature encoding approach offers great promise in automating and optimizing the segmentation of color images.

Analysis of the Tourism Image of Urban Waterfront Nanjing City

The quantitative analysis method was used to measure the tourism image of Nanjing waterfront to make clear its composition.An evaluation method was also adopted in terms of cognitive image,affective image and the whole image,which may offer some references for the evaluation of other tourism images in urban waterfront of the same kind.

Analysis on the Landscape Image of Pingle Old Town in Chengdu City from the Perspective of City Image

With Kevin Lynch's mode of analysis in The Image of the City,through field research and inductive analysis on the Pingle Old Town,the image of Chengdu Pingyuan Town represented by Pingle Town is summarized.Analysis and conclusion of the characteristics and identifiabilities of the traditional market town are made in five aspects,mainly including the path of the town,edge,district,central node and landmark,which provides a theoretical reference and example for preserving and shaping the traditional market town of region characteristic in Chengdu Plain.

Analysis of the Image Space of Rural Landscape of the Pearl River Delta

Through questionnaires and perceptual maps,we made a variation analysis of landscape spatial image to the people in the Pearl River Delta from the viewpoint of the subjective perception.It is found that the people of different ages and different education levels from different areas hold diversified perceptions towards rural landscape space,and they have different ideal rural landscapes.However,there is a common point that the development of rural landscape should retain its excellent local landscape features and the local spirit,which will provide references for building the ideal rural landscape model.

Training image analysis for three-dimensional reconstruction of porous media

In order to obtain a better sandstone three-dimensional （3D） reconstruction result which is more similar to the original sample, an algorithm based on stationarity for a two-dimensional （2D） training image is proposed. The second-order statistics based on texture features are analyzed to evaluate the scale stationarity of the training image. The multiple-point statistics of the training image are applied to obtain the multiple-point statistics stationarity estimation by the multi-point density function. The results show that the reconstructed 3D structures are closer to reality when the training image has better scale stationarity and multiple-point statistics stationarity by the indications of local percolation probability and two-point probability. Moreover, training images with higher multiple-point statistics stationarity and lower scale stationarity are likely to obtain closer results to the real 3D structure, and vice versa. Thus, stationarity analysis of the training image has far-reaching significance in choosing a better 2D thin section image for the 3D reconstruction of porous media. Especially, high-order statistics perform better than low-order statistics.

Analysis of the Tragic Female Images in Eugene O'Neill's Plays

Ruth and Mary are two heroines in Eugene O'Neill's plays Beyond the Horizon, and Long Day's Journey into Night. They have some similarities: when they are young, they are beautiful, native and full of hope towards the future life, but both make wrong choices; in the following years, both suffer a lot from these wrong choices, and feel regretful. This paper tries to explore these two tragic female images.

Numerical Simulation and Analysis of Bipolar Junction Photogate Transistor for CMOS Image Sensor

A new photodetector--bipolar junction photogate transistor is presented for CMOS image sensor and its analytical model is also established.With the technical parameter of the 0.6μm CMOS process,the bipolar junction photogate transistor is analyzed and simulated.The simulated results illustrate that the bipolar junction photogate transistor has the similar characteristics of the traditional photogate transistor.The photocurrent density of the bipolar junction photogate transistor increases exponentially with the incidence light power due to introducing the injection p+n junction.Its characteristic of blue response is rather improved compared to the traditional photogate transistor that benefits to increase the color photograph made up of the red,the green,and the blue.

Principle and Analysis of Novel Gate-Induced Noise in Pixel MOSFET of CMOS Imagers

A detailed principle and a rigorous analysis of a new noise,the gate-induced noise,in pixel MOSFET of CMOS imagers are provided.The gate-induced noise of the MOSFET is more notable in the strong reversion region than that in the subthreshold region when the applied gate voltage is low.However,the applied gate voltage being up to 3V,the gate-induced noise is more notable with the ω/ω T increasing when the MOSFET operates in the subthreshold region than that in the strong reversion region.Between the photocurrent I D and the root mean square value of the gated-induced noise,current i 2 d presents the relation of i 2 d∝I D in the saturation region of the strong reversion and approximately i 2 d∝I D in the subthreshold region.A deta iled and rigorous study of the gate-induced noise in the reset MOSFET for the p hotodiode APS and improved photodiode APS are provided.The improvement of logari thmic response APS is analyzed and the simulation results show that the gate-in duced noise can be reduced.