This paper reviewed the developments of the last ten years in the field of international high-resolution earth observation, and introduced the developmental status and plans for China's high-resolution earth obser...This paper reviewed the developments of the last ten years in the field of international high-resolution earth observation, and introduced the developmental status and plans for China's high-resolution earth observation program. In addition, this paper expounded the transformation mechanism and procedure from earth observation data to geospatial information and geographical knowledge, and examined the key scientific and technological issues, including earth observation networks, high-precision image positioning, image understanding, automatic spatial information extraction, and focus services. These analyses provide a new impetus for pushing the application of China's high-resolution earth observation system from a "quantity" to "quality" change, from China to the world, from providing products to providing online service.展开更多
Aging-related diseases are gradually becoming a major problem with the rapid development of aged population in human society.Although many fluorescent probes have been employed to diagnosis senescence via imaging sene...Aging-related diseases are gradually becoming a major problem with the rapid development of aged population in human society.Although many fluorescent probes have been employed to diagnosis senescence via imaging senescence-associatedβ-galactosidase(SA-β-Gal),which is proved to be closely associated with senescent cells,the similar catalytic effectiveness of enzymatic reaction of ovarian cancer-associatedβ-Gal(OA-β-Gal)will interfere with imaging accuracy.Herein,a near-infrared(NIR)hemicyanine based fluorescent probe HCyXA-βGal was designed for light-up imaging of live cells containingβ-Gal.With the organelle-targeting morpholinyl and positive charge moieties,HCyxA-βGal was successfully applicated to image the difference of enzymatic location in senescent cells and ovarian cancer cells.Furthermore,inspired by the fast response performance,fast and precise imaging of the two cell lines was realized via covering another dimension of fluorescence signal:time-dependent intensity.展开更多
基金supported by National Basic Research Program of China(Grant No. 2012CB719906)
文摘This paper reviewed the developments of the last ten years in the field of international high-resolution earth observation, and introduced the developmental status and plans for China's high-resolution earth observation program. In addition, this paper expounded the transformation mechanism and procedure from earth observation data to geospatial information and geographical knowledge, and examined the key scientific and technological issues, including earth observation networks, high-precision image positioning, image understanding, automatic spatial information extraction, and focus services. These analyses provide a new impetus for pushing the application of China's high-resolution earth observation system from a "quantity" to "quality" change, from China to the world, from providing products to providing online service.
基金supported by National Natural Science Foundation of China(Nos.22122803 and 21788102)the National Natural Science Foundation of Jiangsu Province(No.BK20220644).
文摘Aging-related diseases are gradually becoming a major problem with the rapid development of aged population in human society.Although many fluorescent probes have been employed to diagnosis senescence via imaging senescence-associatedβ-galactosidase(SA-β-Gal),which is proved to be closely associated with senescent cells,the similar catalytic effectiveness of enzymatic reaction of ovarian cancer-associatedβ-Gal(OA-β-Gal)will interfere with imaging accuracy.Herein,a near-infrared(NIR)hemicyanine based fluorescent probe HCyXA-βGal was designed for light-up imaging of live cells containingβ-Gal.With the organelle-targeting morpholinyl and positive charge moieties,HCyxA-βGal was successfully applicated to image the difference of enzymatic location in senescent cells and ovarian cancer cells.Furthermore,inspired by the fast response performance,fast and precise imaging of the two cell lines was realized via covering another dimension of fluorescence signal:time-dependent intensity.
