BACKGROUND: Combined hepatitis B immune globulin (HBIg) and lamivudine in prophylaxis of the recurrence of hepatitis B after liver transplantation has significantly improved the survival of HBsAg positive patients. Th...BACKGROUND: Combined hepatitis B immune globulin (HBIg) and lamivudine in prophylaxis of the recurrence of hepatitis B after liver transplantation has significantly improved the survival of HBsAg positive patients. This study was undertaken to evaluate the outcomes of liver transplantation for patients with hepatitis B virus (HBV). METHODS: A retrospective chart analysis and a review of the organ transplant database identified 51 patients (43 men and 8 women) transplanted for benign HBV-related cirrhotic diseases between June 2002 and December 2004 who had survived more than 3 months. HBIg was administered intravenously during the first week and intramuscularly thereafter. RESULTS: At a median follow-up of 14.1 months, the overall recurrence rate in the 51 patients was 3.9% (2/51). The overall patient survival was 88.3%, and 82.4% after 1 and 2 years, respectively. A daily oral dose of 100 mg lamivudine for 2 weeks before transplantation for 10 patients enabled 57.1% (4/7) and 62.5% (5/8) of HBV-DNA and HBeAg positive patients respectively to convert to be negative. Intramuscular HBIg was well tolerated in all patients. CONCLUSION: Lamivudine combined with intramuscular HBIg can effectively prevent allograft from the recurrence of HBV after liver transplantation.展开更多
Various opinions have been presented on the merits and demerits that breastfeeding gives for the allergic onset of the babies. In this report, we focused on whether food proteins eaten by mother mice and secreted into...Various opinions have been presented on the merits and demerits that breastfeeding gives for the allergic onset of the babies. In this report, we focused on whether food proteins eaten by mother mice and secreted into breast milk as IgA-immune complexes contribute to the allergy prevention through oral tolerance in infants who ingest the milk. BALB/c mice were divided into two groups;E-group fed only egg white proteins and M-group fed only cow’s milk proteins as a dietary protein source. After immunizing M-group infants fed their own mother’s milk with ovalbumin/alum, diarrhea associated with experimental Th2 intestinal inflammation was induced by oral administration of ovalbumin. The diarrhea was dramatically suppressed in E-group infants. Concomitantly, low level of serum anti-ovalbumin- and ovomucoid-IgG1 and IgE, suppression of IL-4 synthesis by spleen cells, and low incidence of anaphylactic death after intravenous injection of ovalbumin were observed preferentially in E-infants. Immune complexes of respective dietary proteins and IgA were found in the breast milk obtained from each group of mother. Oral administration of pseudo immune complex chemically synthesized with ovalbumin and monoclonal mouse IgA in advance effectively suppressed anti-ovalbumin-IgG1 synthesis in adult mice after immunization with ovalbumin. The tolerance induced by the pseudo immune complex of ovalbumin diminished spontaneously while mice did not take egg white proteins. Thus, immune tolerance and then prevention of allergic disorder against dietary proteins were acquired via breastfeeding by mothers feeding the relevant proteins, probably through the immune complexes of dietary proteins and sIgAs secreted into breast milk.展开更多
Objective:To investigate the effects of secretory IgA on the inflammatory response and immune function indicators in children with upper respiratory tract infection.Methods:A total of 80 children with recurrent upper ...Objective:To investigate the effects of secretory IgA on the inflammatory response and immune function indicators in children with upper respiratory tract infection.Methods:A total of 80 children with recurrent upper respiratory tract infection who received treatment in our hospital between September 2017 and September 2018 were retrospectively analyzed and divided into the control group(n=43)receiving conventional anti-infective drug therapy and the sIgA group(n=37)receiving secretory IgA combined with conventional anti-infective drug therapy according to the treatment regimen.The differences in serum contents of inflammatory cytokines and immunoglobulins as well as peripheral blood levels of Th1/Th2 immune response indexes were compared between the two groups before treatment and after 7 days of treatment.Results:Before treatment,there were no significant differences in serum contents of inflammatory cytokines and immunoglobulins or peripheral blood levels of Th1/Th2 immune response indexes between the two groups.After 7 days of treatment,serum inflammatory cytokines tumor necrosis factor-α,interleukin-2 and interleukin-18 contents of the sIgA group were lower than those of the control group;serum immunoglobulin IgE content was lower than that of the control group,while IgG2 and IgG4 contents were higher than those of the control group;peripheral blood Th1 cell distribution proportion and Th1/Th2 ratio were higher than those of the control group,while Th2 cell distribution proportion was lower than that of the control group.Conclusion:Conventional anti-infective drugs combined with secretory IgA can further inhibit the inflammatory response and balance the immune response in children with recurrent upper respiratory tract infections.展开更多
In order to study FcαRⅠ mediated phagocytosis of IgA immune complexes by U937 cells, antigen 8.9NIP/BSA was labeled with FITC and reacted with anti-NIP IgA or anti-NIP IgG antibody to form immune complexes (ICs). Th...In order to study FcαRⅠ mediated phagocytosis of IgA immune complexes by U937 cells, antigen 8.9NIP/BSA was labeled with FITC and reacted with anti-NIP IgA or anti-NIP IgG antibody to form immune complexes (ICs). They were then incubated with phorbol 12-myristate 13-acetate (PMA) stimulated U937 cells.The phagocytosed ICs were quantified by flow cytometry. The results was that the expression of FcαRⅠ on U937 cells was higher than that of FcγRⅠ, FcγRⅡ and FcγRⅢ. After stimulation by PMA, expression of FcαRⅠ on U937 cells was markedly upregulated and the phagocytosis of IgA ICs was enhanced. FcαRⅠ mediated specific IgA phagocytosis was stronger than FcγRⅠ and FcγRⅡ mediated IgG phagocytosis. Complement receptors, CR1 and CR3, enhanced U937 cell phagocytosis of IgA ICs. It concludes that FcαRⅠ mediated strong phagocytosis of IgA ICs.展开更多
文摘BACKGROUND: Combined hepatitis B immune globulin (HBIg) and lamivudine in prophylaxis of the recurrence of hepatitis B after liver transplantation has significantly improved the survival of HBsAg positive patients. This study was undertaken to evaluate the outcomes of liver transplantation for patients with hepatitis B virus (HBV). METHODS: A retrospective chart analysis and a review of the organ transplant database identified 51 patients (43 men and 8 women) transplanted for benign HBV-related cirrhotic diseases between June 2002 and December 2004 who had survived more than 3 months. HBIg was administered intravenously during the first week and intramuscularly thereafter. RESULTS: At a median follow-up of 14.1 months, the overall recurrence rate in the 51 patients was 3.9% (2/51). The overall patient survival was 88.3%, and 82.4% after 1 and 2 years, respectively. A daily oral dose of 100 mg lamivudine for 2 weeks before transplantation for 10 patients enabled 57.1% (4/7) and 62.5% (5/8) of HBV-DNA and HBeAg positive patients respectively to convert to be negative. Intramuscular HBIg was well tolerated in all patients. CONCLUSION: Lamivudine combined with intramuscular HBIg can effectively prevent allograft from the recurrence of HBV after liver transplantation.
文摘Various opinions have been presented on the merits and demerits that breastfeeding gives for the allergic onset of the babies. In this report, we focused on whether food proteins eaten by mother mice and secreted into breast milk as IgA-immune complexes contribute to the allergy prevention through oral tolerance in infants who ingest the milk. BALB/c mice were divided into two groups;E-group fed only egg white proteins and M-group fed only cow’s milk proteins as a dietary protein source. After immunizing M-group infants fed their own mother’s milk with ovalbumin/alum, diarrhea associated with experimental Th2 intestinal inflammation was induced by oral administration of ovalbumin. The diarrhea was dramatically suppressed in E-group infants. Concomitantly, low level of serum anti-ovalbumin- and ovomucoid-IgG1 and IgE, suppression of IL-4 synthesis by spleen cells, and low incidence of anaphylactic death after intravenous injection of ovalbumin were observed preferentially in E-infants. Immune complexes of respective dietary proteins and IgA were found in the breast milk obtained from each group of mother. Oral administration of pseudo immune complex chemically synthesized with ovalbumin and monoclonal mouse IgA in advance effectively suppressed anti-ovalbumin-IgG1 synthesis in adult mice after immunization with ovalbumin. The tolerance induced by the pseudo immune complex of ovalbumin diminished spontaneously while mice did not take egg white proteins. Thus, immune tolerance and then prevention of allergic disorder against dietary proteins were acquired via breastfeeding by mothers feeding the relevant proteins, probably through the immune complexes of dietary proteins and sIgAs secreted into breast milk.
基金This study was supported by the Science and Technology Key Project(Grant No:2017ks-04).
文摘Objective:To investigate the effects of secretory IgA on the inflammatory response and immune function indicators in children with upper respiratory tract infection.Methods:A total of 80 children with recurrent upper respiratory tract infection who received treatment in our hospital between September 2017 and September 2018 were retrospectively analyzed and divided into the control group(n=43)receiving conventional anti-infective drug therapy and the sIgA group(n=37)receiving secretory IgA combined with conventional anti-infective drug therapy according to the treatment regimen.The differences in serum contents of inflammatory cytokines and immunoglobulins as well as peripheral blood levels of Th1/Th2 immune response indexes were compared between the two groups before treatment and after 7 days of treatment.Results:Before treatment,there were no significant differences in serum contents of inflammatory cytokines and immunoglobulins or peripheral blood levels of Th1/Th2 immune response indexes between the two groups.After 7 days of treatment,serum inflammatory cytokines tumor necrosis factor-α,interleukin-2 and interleukin-18 contents of the sIgA group were lower than those of the control group;serum immunoglobulin IgE content was lower than that of the control group,while IgG2 and IgG4 contents were higher than those of the control group;peripheral blood Th1 cell distribution proportion and Th1/Th2 ratio were higher than those of the control group,while Th2 cell distribution proportion was lower than that of the control group.Conclusion:Conventional anti-infective drugs combined with secretory IgA can further inhibit the inflammatory response and balance the immune response in children with recurrent upper respiratory tract infections.
文摘In order to study FcαRⅠ mediated phagocytosis of IgA immune complexes by U937 cells, antigen 8.9NIP/BSA was labeled with FITC and reacted with anti-NIP IgA or anti-NIP IgG antibody to form immune complexes (ICs). They were then incubated with phorbol 12-myristate 13-acetate (PMA) stimulated U937 cells.The phagocytosed ICs were quantified by flow cytometry. The results was that the expression of FcαRⅠ on U937 cells was higher than that of FcγRⅠ, FcγRⅡ and FcγRⅢ. After stimulation by PMA, expression of FcαRⅠ on U937 cells was markedly upregulated and the phagocytosis of IgA ICs was enhanced. FcαRⅠ mediated specific IgA phagocytosis was stronger than FcγRⅠ and FcγRⅡ mediated IgG phagocytosis. Complement receptors, CR1 and CR3, enhanced U937 cell phagocytosis of IgA ICs. It concludes that FcαRⅠ mediated strong phagocytosis of IgA ICs.
