AIM: To detect immunohistochemically the presence of oval cells in chronic viral hepatitis with antibody against c-kit. METHODS: We detected oval cells in paraffin embedded liver sections of 3 normal controls and 26 l...AIM: To detect immunohistochemically the presence of oval cells in chronic viral hepatitis with antibody against c-kit. METHODS: We detected oval cells in paraffin embedded liver sections of 3 normal controls and 26 liver samples from patients with chronic viral hepatitis, using immunohistochemistry with antibodies against c-kit, piclass glutathione S-transferase (pi-GST) and cytokeratins 19 (CK19). RESULTS: Oval cells were not observed in normal livers. In chronic viral hepatitis, hepatic oval cells were located predominantly in the periportal region and fibrosis septa,characterized by an ovoid nucleus, small size,and scant cytoplasm. Antibody against stem cell factor receptor, c-kit, had higher sensitivity and specificity than pi-GST and CK19. About 50%-70% of c-kit positive oval cells were stained positively for either pi-GST or CK19. CONCLUSION: Oval cells are frequently detected in human livers with chronic viral hepatitis, suggesting that oval cell proliferation is associated with the liver regeneration in this condition.展开更多
AIM: To assess the possibility of non-invasive screening of atrophic chronic gastritis for preventing further development of gastric cancer.METHODS: One hundred and seventy-eight consecutive Helicobacter pylori ( H py...AIM: To assess the possibility of non-invasive screening of atrophic chronic gastritis for preventing further development of gastric cancer.METHODS: One hundred and seventy-eight consecutive Helicobacter pylori ( H pylori)-positive dyspeptic patients after detection of serum levels of pepsinogen-1 (PG-1) and gastrin-17 (G-17) by enzyme immunoassay were proposed for endoscopy and histology. The serologic and morphologic results were compared with estimating the sensitivity, specificity and prognostic values of the tests.RESULTS: There was statistically significant reverse dependence between the grade of stomach mucosal antral or corpus atrophy and the proper decreasing of serum G17 or PG1 levels. The serologic method was quite sensitive in the diagnosis of non-atrophic and severe antral and corpus gastritis. Also, it was characterized by the high positive and negative prognostic values.CONCLUSION: Detection of serum G-17 and PG1 levels can be offered as the screening tool for atrophic gastritis. The positive serologic results require further chromoendoscopy with mucosal biopsy, for revealing probable progressing of atrophic process with development of intestinal metaplasia, dysplasia or gastric cancer.展开更多
Objective: To investigate the distribution of the popula- tion with iow level HBsAg in serum and the characteri- stics of their HBV serologic markers. Methods: We detected HBsAg, HBeAg, anti-HBc, an- ti-HBe, and anti-...Objective: To investigate the distribution of the popula- tion with iow level HBsAg in serum and the characteri- stics of their HBV serologic markers. Methods: We detected HBsAg, HBeAg, anti-HBc, an- ti-HBe, and anti-HBs in the serum samples from 8089 non-hepatitis inpatients and defined the patients with HBsAg concentration equal to or less than 5 μg/L by using the AxSYM microparticle enzyme immunoassay (MEIA) system. Results: 189 patients with HBsAg equal to or Iow than 5 μg/L (2.34% of all 8089 patients and 23.16% of 816 HBsAg positive patients) included 84 (44.40%), 33 (17.5%) and 72 (38.10%) with HBsAg equal to or less than 1 μg/L, 1-2 μg/L and 2-5 μg/L respec- tively. Most of these patients were detected with 'posi- tive for HBsAg, anti-HBc and anti-HBe but negative for HBeAg and anti-HBs', and anti-HBc antibodies were frequently present in their sera. Conclusions: The population with low Level HBsAg in serum should not be neglected and it is important to improve the test sensitivity of HBsAg and to detect the other HBV serologic markers.展开更多
Thirty-six cases originally diagnosed histologically as malignant lymphoma (ML) or anaplastic carcinoma were reexamined immunohistochemically by leukocyte common antigen (LCA) and epithelial membrane antigen (EMA). 5/...Thirty-six cases originally diagnosed histologically as malignant lymphoma (ML) or anaplastic carcinoma were reexamined immunohistochemically by leukocyte common antigen (LCA) and epithelial membrane antigen (EMA). 5/18 cases originally diagnosed as ' anaplastic carcinoma'' and 'anaplastic carcinoma? (ML to be excluded)' , showed positive staining for LCA and negative for EMA; whereas 3 cases originally diagnosed as malignancy unclassified turned out to be ML, with the relevant assay performed.展开更多
文摘AIM: To detect immunohistochemically the presence of oval cells in chronic viral hepatitis with antibody against c-kit. METHODS: We detected oval cells in paraffin embedded liver sections of 3 normal controls and 26 liver samples from patients with chronic viral hepatitis, using immunohistochemistry with antibodies against c-kit, piclass glutathione S-transferase (pi-GST) and cytokeratins 19 (CK19). RESULTS: Oval cells were not observed in normal livers. In chronic viral hepatitis, hepatic oval cells were located predominantly in the periportal region and fibrosis septa,characterized by an ovoid nucleus, small size,and scant cytoplasm. Antibody against stem cell factor receptor, c-kit, had higher sensitivity and specificity than pi-GST and CK19. About 50%-70% of c-kit positive oval cells were stained positively for either pi-GST or CK19. CONCLUSION: Oval cells are frequently detected in human livers with chronic viral hepatitis, suggesting that oval cell proliferation is associated with the liver regeneration in this condition.
文摘AIM: To assess the possibility of non-invasive screening of atrophic chronic gastritis for preventing further development of gastric cancer.METHODS: One hundred and seventy-eight consecutive Helicobacter pylori ( H pylori)-positive dyspeptic patients after detection of serum levels of pepsinogen-1 (PG-1) and gastrin-17 (G-17) by enzyme immunoassay were proposed for endoscopy and histology. The serologic and morphologic results were compared with estimating the sensitivity, specificity and prognostic values of the tests.RESULTS: There was statistically significant reverse dependence between the grade of stomach mucosal antral or corpus atrophy and the proper decreasing of serum G17 or PG1 levels. The serologic method was quite sensitive in the diagnosis of non-atrophic and severe antral and corpus gastritis. Also, it was characterized by the high positive and negative prognostic values.CONCLUSION: Detection of serum G-17 and PG1 levels can be offered as the screening tool for atrophic gastritis. The positive serologic results require further chromoendoscopy with mucosal biopsy, for revealing probable progressing of atrophic process with development of intestinal metaplasia, dysplasia or gastric cancer.
文摘Objective: To investigate the distribution of the popula- tion with iow level HBsAg in serum and the characteri- stics of their HBV serologic markers. Methods: We detected HBsAg, HBeAg, anti-HBc, an- ti-HBe, and anti-HBs in the serum samples from 8089 non-hepatitis inpatients and defined the patients with HBsAg concentration equal to or less than 5 μg/L by using the AxSYM microparticle enzyme immunoassay (MEIA) system. Results: 189 patients with HBsAg equal to or Iow than 5 μg/L (2.34% of all 8089 patients and 23.16% of 816 HBsAg positive patients) included 84 (44.40%), 33 (17.5%) and 72 (38.10%) with HBsAg equal to or less than 1 μg/L, 1-2 μg/L and 2-5 μg/L respec- tively. Most of these patients were detected with 'posi- tive for HBsAg, anti-HBc and anti-HBe but negative for HBeAg and anti-HBs', and anti-HBc antibodies were frequently present in their sera. Conclusions: The population with low Level HBsAg in serum should not be neglected and it is important to improve the test sensitivity of HBsAg and to detect the other HBV serologic markers.
文摘Thirty-six cases originally diagnosed histologically as malignant lymphoma (ML) or anaplastic carcinoma were reexamined immunohistochemically by leukocyte common antigen (LCA) and epithelial membrane antigen (EMA). 5/18 cases originally diagnosed as ' anaplastic carcinoma'' and 'anaplastic carcinoma? (ML to be excluded)' , showed positive staining for LCA and negative for EMA; whereas 3 cases originally diagnosed as malignancy unclassified turned out to be ML, with the relevant assay performed.
