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Introduction Performance and Cultivation Techniques of Himrod Grape Planted in Tianjin
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作者 黄建全 聂松青 +2 位作者 温晓敏 路凤珍 田淑芬 《Agricultural Science & Technology》 CAS 2017年第6期999-1001,共3页
After six years of Himrod grape regional trial, the Himrod had very early- ripening precocity, stable flower bud differentiation, good fruit quality and great resis- tance to diseases, which could be a moderate develo... After six years of Himrod grape regional trial, the Himrod had very early- ripening precocity, stable flower bud differentiation, good fruit quality and great resis- tance to diseases, which could be a moderate development grape variety in Tianjin. The introduction performance cultivation techniques of Himrod grape were summa- rized. 展开更多
关键词 GRAPE Himrod Introduction performance ADAPTABILITY Cultivation tech- niques
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Notch1胞内段在鼻咽癌组织及鼻咽癌细胞株中的表达研究 被引量:6
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作者 张芳婷 万汇涓 +4 位作者 李明华 刘晓翌 尹美珺 叶静 于洁 《中国全科医学》 CAS CSCD 北大核心 2012年第24期2782-2784,共3页
目的探讨Notch1胞内段(NIC)在非角化性鼻咽癌组织和细胞株中的表达。方法采用反转录聚合酶链反应(RT-PCR)及免疫组化染色方法对鼻咽癌标本及不同分化程度鼻咽癌细胞株进行检测。结果 RT-PCR检测显示不同鼻咽癌细胞株中NIC基因均有表达,... 目的探讨Notch1胞内段(NIC)在非角化性鼻咽癌组织和细胞株中的表达。方法采用反转录聚合酶链反应(RT-PCR)及免疫组化染色方法对鼻咽癌标本及不同分化程度鼻咽癌细胞株进行检测。结果 RT-PCR检测显示不同鼻咽癌细胞株中NIC基因均有表达,但分化程度高的CNE1细胞株表达最明显;同期进行的免疫组化染色结果与之相似;对鼻咽癌标本的免疫组化染色结果显示:鼻咽癌组织中NIC的表达高于鼻咽慢性黏膜炎症组织(P=0.005),而且NIC的表达强度与细胞分化程度密切相关(P<0.05),与患者性别、年龄无相关性(P>0.05)。另外,在未分化型鼻咽癌中,癌巢内几乎无阳性表达,淋巴细胞阳性表达多;而在分化型鼻咽癌中癌细胞阳性表达明显,可见于胞膜、胞质及胞核。结论 Notch信号系统参与鼻咽癌的发生,其中Notch1的表达与鼻咽癌的分化程度相关。 展开更多
关键词 非角化性鼻咽癌 Notch1胞内段 免疫组化染色 反转录聚合酶链反应
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血管内皮生长因子在肝细胞癌中的表达及其临床价值
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作者 万骋 崔斐 +3 位作者 陈斌 陈锦章 黄宇贤 罗荣城 《河北医学》 CAS 2008年第6期638-640,共3页
目的:探讨肝细胞肝癌(HCC)组织中血管内皮生长因子(VEGF)的表达相关性及其临床意义。方法:应用免疫组织化学方法检测50例HCC组织和20例正常肝组织中VEGF蛋白的表达,并将检测结果与临床病理特征进行综合分析。结果:①50例HCC组织中VEGF... 目的:探讨肝细胞肝癌(HCC)组织中血管内皮生长因子(VEGF)的表达相关性及其临床意义。方法:应用免疫组织化学方法检测50例HCC组织和20例正常肝组织中VEGF蛋白的表达,并将检测结果与临床病理特征进行综合分析。结果:①50例HCC组织中VEGF蛋白的阳性表达率为68.0%(34/50),在正常肝组织中的表达率为10%(2/20),差异有显著性;②VEGF蛋白表达与患者的性别、年龄、肿瘤大小、分化程度,AFP值无相关性(P>0.05),而与临床分期及转移相关(P<0.05)。结论:VEGF蛋白在HCC中表达率较高,为肝癌的分子靶向治疗提供了新的靶点;VEGF在HCC中的表达关系密切,测定HCC中VEGF蛋白的表达,有助于临床判断病人预后。 展开更多
关键词 肝细胞癌 VEGF 靶向治疗 免疫组化
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HER2基因扩增和蛋白表达与胃癌临床病理因素的相关性
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作者 余娟 张明智 《河南医学研究》 CAS 2014年第2期4-6,共3页
目的:探讨HER2基因扩增和蛋白表达与胃癌不同临床因素的相关性。方法:回顾性分析河南省肿瘤医院2012年5月至2013年10月收治的1 002例胃癌患者术后肿瘤组织病理标本,应用免疫组化技术(IHC法)和双色银染原位杂交技术(SISH法)检测HER2基因... 目的:探讨HER2基因扩增和蛋白表达与胃癌不同临床因素的相关性。方法:回顾性分析河南省肿瘤医院2012年5月至2013年10月收治的1 002例胃癌患者术后肿瘤组织病理标本,应用免疫组化技术(IHC法)和双色银染原位杂交技术(SISH法)检测HER2基因扩增和蛋白表达情况,比较不同临床特点的胃癌患者亚组间HER2表达阳性率的差异。结果:1 002例术后肿瘤组织病理标本中HER2阳性208例(20.8%)。HER2蛋白过表达与Lauren分型、病理分化程度和浸润深度有明显的相关性,差异均有统计学意义(P<0.05)。结论:在肠型胃癌、分化较好的胃癌、胃癌全层浸润时HER2阳性率明显提高。 展开更多
关键词 胃癌 人表皮生长因子受体2 免疫组化技术 双色银染原位杂交技术
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Preliminary study of a dispersed fringe type sensing system 被引量:3
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作者 Yong Zhang Gen-Rong Liu +3 位作者 Yue-Fei Wang Ye-Ping Li Ya-Jun Zhang Liang Zhan-g 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2009年第8期945-952,共8页
Telescopes with large aspherical primary mirrors collect more light and are therefore sought after by astronomers. Instead of using a single large one-piece mirror, smaller segments can be assembled into a useable tel... Telescopes with large aspherical primary mirrors collect more light and are therefore sought after by astronomers. Instead of using a single large one-piece mirror, smaller segments can be assembled into a useable telescopic primary. Because the segments must fit together to create the effect of a single mirror, segmented optics present unique challenges to the fabrication and testing that are absent in monolithic optics. A dispersed fringe sensor (DFS) using a broadband point source is an efficient method for cophasing and is also highly automated and robust. Unlike the widely adopted Shack- Hartmann Wavefront sensor and curvature wavefront sensor with edge sensors for calibration of relative pistons, DFS can estimate the piston between segments by only using the spectrum formed by the transmissive grating's dispersion, and therefore can replace the edge sensors, which are difficult to calibrate. We introduce the theory of the DFS and Dispersed Hartmann Sensor (DHS) for further utilization of the coarse phasing method of DFS. According to the theory, we bring out the preliminary system design of the cophasing experimental system based on DFS and DHS which is now established in our institute. Finally, a summary is reached. 展开更多
关键词 INSTRUMENTATION active optics -- techniques image processing -- tech- niques spectroscopic -- telescope cophasing sensor
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Effects of Skeletonized versus Pedicled Internal Thoracic Artery Grafts on Free Flow Capacity during Bypass
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作者 黄畦 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2000年第4期308-310,共3页
The free flow of skeletonized ITA grafts was compared with that of pedicled ITA grafts. One hundred patients with coronary artery diseases underwent elective CABG. In the group Ⅰ(n= 50), the left ITA was dissected u... The free flow of skeletonized ITA grafts was compared with that of pedicled ITA grafts. One hundred patients with coronary artery diseases underwent elective CABG. In the group Ⅰ(n= 50), the left ITA was dissected using the skeletonization technique. In the group Ⅱ(n=50), the ITA was harvested as a pedicled graft. Free flow of the ITA was recorded before and 15 min after in- traluminal application of diluted papaverine. Mean arterial pressure was maintained at 9. 31 kPa (70 mmHg). The results showed that before the application of papaverine, free flow of skeletonized and pedicled ITA grafts was identical between the two groups. After treatment with papaverine, the maximum free flow was significantly higher in the skeletonized ITA's in the group Ⅰ(199. 3±69. 6 ml/min) than in the group Ⅱ(145. 7±70. 3 ml/min, P<0. 05). There was on significant differ- ence between the free flow after dilatation of the left and right ITA in the group Ⅰ (left 199. 3±69. 6 ml/mm, right 198. 9±61. 8 ml/mm, respectively). It was concluded that preparation of the ITA with the skeletonization technique resulted in significantly higher free flow capacity than in pedicled grafts and would improve the results of arterial revascularization. The complication rate seems to be lower than with the conventional method. 展开更多
关键词 coronary surgery internal thoracic artery SKELETONIZATION vessel preparation tech- nique
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Surgical correction of postductal aortic coarctation in 40 adolescents and adults: A ten-year single institution experience
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作者 李欣 杨珏 +2 位作者 于长江 卿洪琨 范瑞新 《South China Journal of Cardiology》 CAS 2017年第2期106-113,共8页
Background In clinical practice, there are different surgical approaches for postductal coarctation of the aor- ta (CoA), with their advantages and disadvantages. Limited studies have reported the surgical outcomes ... Background In clinical practice, there are different surgical approaches for postductal coarctation of the aor- ta (CoA), with their advantages and disadvantages. Limited studies have reported the surgical outcomes of post- ductal CoA in adolescents and adults. Methods From January 2005 to December 2014, a total of 40 patients aged over 14 years underwent surgical corrections of postductal CoA in our institution. The surgical outcomes as reflected by cardiac function and differences in mean blood pressure of upper and lower extremities both preoper- ative and postoperative were recorded and evaluated. Results Among the 40 patients underwent successful sur- gical corrections, 1 patient complicated by acute aortic dissection and died, while the remainings survived to hos- pital discharge. During the 12-36 month follow-up period, postoperative mean blood pressure differences of upper and lower extremities were significantly reduced as compared with the preoperative data. Postoperative evalu- ation of cardiac function was conducted in all patients with New York Heart Association (NYHA) Ⅰ-Ⅱ. Conclusion For adolescent and adult patients with postductal aortic coarctation, surgical correction is highly recom- mended. Surgical approach should be based on the specific anatomy of the coarctation lesion, concomitant mal- formations, and expected grown-up height. Those complicated with other intracardiac malformations should be treated with extra-anatomical bypass technique and simultaneous surgical correction of CoA, which is effective with desirable postoperative prognosis. 展开更多
关键词 coarctation of the aorta (CoA) congenital heart disease in adult extra-anatomical bypass tech- nique surgical treatment
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