AIM:To investigate expression of factors related to angiogenesis:HIF-1α,iNOS,COX-2 and VEGF in choroidal melanoma and its clinical significance.· METHODS:Fifty samples of choroidal melanoma and 15 samples of mel...AIM:To investigate expression of factors related to angiogenesis:HIF-1α,iNOS,COX-2 and VEGF in choroidal melanoma and its clinical significance.· METHODS:Fifty samples of choroidal melanoma and 15 samples of melanocytic nevi of the eyelid identified by pathology were collected.Immunohistochemistry SP method was used to examine the expression of HIF-1α,iNOS,COX-2 and VEGF in these samples.The comparison among groups was done by SPSS 13.0 software.· RESULTS:The positive expression rates of HIF-1α,iNOS,COX-2 and VEGF in choroidal melanoma group were significantly higher than those in eyelid nevi group(χ2= 6.5542,7.7224,8.5828,15.1749).The positive expression rate of VEGF was associated with the tumor size(χ2= 10.9194),but was not associated with pathological type(χ2=2.0712) and the situation of scleral invasion(χ2= 5.4289).The positive expression rate of HIF-1α was associated with the tumor size(χ2=7.1216) and pathological type(χ2=9.0889),but was not associated with the situation of scleral invasion(χ2=3.3586).The positive expression rate of iNOS was associated with the tumor size(χ2=9.5503),but was not associated with pathological type(χ2=1.9450) and the situation of scleral invasion(χ2=2.3810).The positive expression rate of COX-2 was associated with the tumor size(χ2=7.2970),but was not associated with pathological type(χ2=1.8421) and the situation of scleral invasion(χ2= 0.4018).The expression of HIF-1α,iNOS and COX-2 were significantly associated with the expression of VEGF(r=0.9429,1,0.9857).The expression of COX-2 was significantly associated with the expression of iNOS(r=0.9857).The expression of HIF-1α was significantly associated with the expression of COX-2(r =0.9857).The expression of HIF-1α was significantly associated with the expression of iNOS(r = 0.9429).· CONCLUSION:The expression of HIF-1α,iNOS and COX-2 protein in choroidal melanoma were higher and may relate to angiogenesis and stimulate tumor growth.Determination of HIF-1α,iNOS and COX-2 may be helpful for the diagnosis and therapy of this tumor.展开更多
文摘AIM:To investigate expression of factors related to angiogenesis:HIF-1α,iNOS,COX-2 and VEGF in choroidal melanoma and its clinical significance.· METHODS:Fifty samples of choroidal melanoma and 15 samples of melanocytic nevi of the eyelid identified by pathology were collected.Immunohistochemistry SP method was used to examine the expression of HIF-1α,iNOS,COX-2 and VEGF in these samples.The comparison among groups was done by SPSS 13.0 software.· RESULTS:The positive expression rates of HIF-1α,iNOS,COX-2 and VEGF in choroidal melanoma group were significantly higher than those in eyelid nevi group(χ2= 6.5542,7.7224,8.5828,15.1749).The positive expression rate of VEGF was associated with the tumor size(χ2= 10.9194),but was not associated with pathological type(χ2=2.0712) and the situation of scleral invasion(χ2= 5.4289).The positive expression rate of HIF-1α was associated with the tumor size(χ2=7.1216) and pathological type(χ2=9.0889),but was not associated with the situation of scleral invasion(χ2=3.3586).The positive expression rate of iNOS was associated with the tumor size(χ2=9.5503),but was not associated with pathological type(χ2=1.9450) and the situation of scleral invasion(χ2=2.3810).The positive expression rate of COX-2 was associated with the tumor size(χ2=7.2970),but was not associated with pathological type(χ2=1.8421) and the situation of scleral invasion(χ2= 0.4018).The expression of HIF-1α,iNOS and COX-2 were significantly associated with the expression of VEGF(r=0.9429,1,0.9857).The expression of COX-2 was significantly associated with the expression of iNOS(r=0.9857).The expression of HIF-1α was significantly associated with the expression of COX-2(r =0.9857).The expression of HIF-1α was significantly associated with the expression of iNOS(r = 0.9429).· CONCLUSION:The expression of HIF-1α,iNOS and COX-2 protein in choroidal melanoma were higher and may relate to angiogenesis and stimulate tumor growth.Determination of HIF-1α,iNOS and COX-2 may be helpful for the diagnosis and therapy of this tumor.
