AIM: To investigate FasL expression in hilar cholangiocarcinoma tissues and cultured cholangiocarcinoma cells, and to assess its ability to induce apoptosis. METHODS: We studied the expression of FasL by human hilar c...AIM: To investigate FasL expression in hilar cholangiocarcinoma tissues and cultured cholangiocarcinoma cells, and to assess its ability to induce apoptosis. METHODS: We studied the expression of FasL by human hilar cholangiocaroinomas tissues by immunohistochemistry, and the QBC939 cholangiocarcinoma cell line by RT-PCR, immunohistochemistry, and Western Blot. TUNEL and flow cytometry were used to detect apoptotic cells. RESULTS: Prevalent expression of FasL was detected in 39 resected hilar cholangiocarcinoma tissues. TUNEL staining disclosed a high level of cell death among lymphocytes infiltrating FasL positive areas of tumor. FasL mRNA and protein expressions in cholangiocarcinoma cells could induce Jurkat cells. CONCLUSION: Hilar cholangiocarcinomas may elude immunological surveillance by inducing, via Fas/FasL system, the apoptosis of activated lymphocytes.展开更多
The vulnerability of healthcare and laboratory to potential infection by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus has thus far been analyzed through the lens of the acute phase of the pan-d...The vulnerability of healthcare and laboratory to potential infection by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus has thus far been analyzed through the lens of the acute phase of the pan-demic,including remote-based work,as well as emergency settings that are different from routine healthcare operations.However,as lockdowns ease and activities return to an identifiable pre-pandemic routine,the safety considerations also require to shift accordingly.As laboratory workers are likely to continue being exposed to unidentified SARS-CoV-2 positive samples through routine blood collection and processing opera-tions,coronavirus disease 2019(COVID-19)might have to be re-considered as an occupational disease within this context.Additionally,as per many such occupational diseases,a surveillance system is implemented for the medium-and long-term.This manuscript presents the views on the possible surveillance scenarios for labora-tory staff,viewed from an immunological and biosafety perspective.展开更多
文摘AIM: To investigate FasL expression in hilar cholangiocarcinoma tissues and cultured cholangiocarcinoma cells, and to assess its ability to induce apoptosis. METHODS: We studied the expression of FasL by human hilar cholangiocaroinomas tissues by immunohistochemistry, and the QBC939 cholangiocarcinoma cell line by RT-PCR, immunohistochemistry, and Western Blot. TUNEL and flow cytometry were used to detect apoptotic cells. RESULTS: Prevalent expression of FasL was detected in 39 resected hilar cholangiocarcinoma tissues. TUNEL staining disclosed a high level of cell death among lymphocytes infiltrating FasL positive areas of tumor. FasL mRNA and protein expressions in cholangiocarcinoma cells could induce Jurkat cells. CONCLUSION: Hilar cholangiocarcinomas may elude immunological surveillance by inducing, via Fas/FasL system, the apoptosis of activated lymphocytes.
文摘The vulnerability of healthcare and laboratory to potential infection by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus has thus far been analyzed through the lens of the acute phase of the pan-demic,including remote-based work,as well as emergency settings that are different from routine healthcare operations.However,as lockdowns ease and activities return to an identifiable pre-pandemic routine,the safety considerations also require to shift accordingly.As laboratory workers are likely to continue being exposed to unidentified SARS-CoV-2 positive samples through routine blood collection and processing opera-tions,coronavirus disease 2019(COVID-19)might have to be re-considered as an occupational disease within this context.Additionally,as per many such occupational diseases,a surveillance system is implemented for the medium-and long-term.This manuscript presents the views on the possible surveillance scenarios for labora-tory staff,viewed from an immunological and biosafety perspective.
