Electrochemical Behavior of Adriamycin at Ni/GC Ion Implantation Modified Electrode

With a Ni/GC ion implantation modified electrode as working electrode, in 0.1 mol/L HOAc-NaOAc (pH=4.62) solution, a sensitive reductive wave of ADM was obtained by linear sweep voltammetry. The peak potential was -0.55 V (vs.SCE). The peak current is proportional to the concentration of ADM with a detection limit of 6.9 X 10(-8) mol/L. The behavior of the reduction wave was studied. The experiments of AES and XPS showed that Ni was surely implanted into the surface of the GCE and the implanted Ni at the GCE improved the electrocatalytic activity.

Studies on the Electrochemical Behavior of Bleomycin at a Co/GC Ion Implantation Modified Electrode and Its Application

In a 0. 10 mol/L HAc-NaAc buffer solution (pH = 4. 59), a sensitive reduction peak of bleomycin was observed by linear sweep voltammetry at a Co/GC ion implantation modified electrode. The peak potential was-0. 73 V(iw. SCE). The peak current was proportional to the concentration of bleomycin over the range of 5.0 × 10-8-1.0× 10-6 and 1.0× 10-6-1. 0 × 10-5 mol/L with a detection limit of 2.0 × 10-8 mol/L. The electrochemical behavior of the reduction peak of bleomycin at the Co/GC modified electrode was studied by linear sweep and cyclic voltammetry and applied to the determination of bleomycin in urine. This method is simple, rapid and reliable. The reduction process is quasi-reversible. The experiments of AES and XPS showed that Co was surely implanted into the surface of GCE and the depth distribution of Co was in good agreement with Gooses normal distribution; the implanted Co at GCE improved the electrocatalytic activity.

Visual evoked potential by implanted scalp electrode in rats

·AIM:To establish a new method to record visual evoked potential of rats and this method was applied to retinal cone degeneration (RCD) and congenital stationary night blindness (CSNB) rats to see whether it is feasible.·METHODS:After anesthesia,the rats scalp surface was exposed and two stainless screws were implanted at 4mm in front of and 8mm behind Bregma.The front one was reference electrode and the latter one was recording electrode.The ground electrode was joined to the tail.And we recorded VEP of scotopic adaptation and photopic adaptation (The stimulus intensity of light :0.011,0.035,0.11,0.35,1.1 and 3.5cd·s/m2.They were all overlapped for 100 times.There were 2-5minutes intervals between different intensities.The frequency of stimulation was 1Hz and time course was 300 milliseconds).A week later,we recorded the VEP again in the same conditions.·RESULTS:The rats who were implanted with electrodes could live longer.When they were recorded,there was no disturbance and the waveforms were very clear and steady.There was no difference between the waveforms recorded by conventional method and those by implanted electrodes while the amplitude of new method was larger than conventional.We could get the same conclusion in RCD and CSNB rats.·CONCLUSION:Since the electrodes can be kept on the scalp for a longer time,we can not only observe dynamic changes of VEP but also observe the long-duration patho-logical changes in the rats.Because the positions of recording and reference electrodes were fixed,the system error can be reduced and the repeatability of experiments increased.·

A Miniaturized System for Neural Signal Acquiring and Processing

To collect neural activity data from awake, behaving freely animals, we develop miniaturized implantable recording system by the modem chip：Programmable System on Chip （PSoC） and through chronic electrodes in the cortex. With PSoC family member CY8C29466,the system completed operational and instrument amplifiers, filters, timers, AD convertors, and serial communication, etc. The signal processing was dealt with virtual instrument technology. All of these factors can significantly affect the price and development cycle of the project. The result showed that the system was able to record and analyze neural extrocellular discharge generated by neurons continuously for a week or more. This is very useful for the interdisciplinary research of neuroscience and information engineering technique. The circuits and architecture of the devices can be adapted for neurobiology and research with other small animals.

A Radio Communication System for Neuronal Signals

To collect neuronal activity data from awake, freely behaving animals, we developed miniature telemetry recording system. The integrated system consists of four major components： l） Microelectrodes and micro-driver assembly, 2） analog front end （AFE）, 3） programmable system on chip （PSoC）, and 4） ra- dio transceiver and the LabVIEW were used as a platform for the graphic user interface. The result showed the system was able to record and analyze neuronal recordings in freely moving animals and lasted continuously for a time period of a week or more. This is very useful for the study of the interdisciplinary research of neu- roscience and information engineering techniques. The circuits and architecture of the devices can be adapted for neurobiology and research with other small animals.

Nerve function restoration following targeted muscle reinnervation after varying delayed periods

Targeted muscle reinnervation has been proposed for reconstruction of neuromuscular function in amputees.However,it is unknown whether performing delayed targeted muscle reinnervation after nerve injury will affect restoration of function.In this rat nerve injury study,the median and musculocutaneous nerves of the forelimb were transected.The proximal median nerve stump was sutured to the distal musculocutaneous nerve stump immediately and 2 and 4 weeks after surgery to reinnervate the biceps brachii.After targeted muscle reinnervation,intramuscular myoelectric signals from the biceps brachii were recorded.Signal amplitude gradually increased with time.Biceps brachii myoelectric signals and muscle fiber morphology and grooming behavior did not significantly differ among rats subjected to delayed target muscle innervation for different periods.Targeted muscle reinnervation delayed for 4 weeks can acquire the same nerve function restoration effect as that of immediate reinnervation.

Studies on Electrochemical Behavior of Bleomycin and Its Interaction with DNA at a Co/GC Ion Implantation Modified Electrode

Electrochemical investigation of Bleomycin (BLM) and its interaction with DNAat a Co/GC ion implantation modified electrode was reported for the first time. The electrochemicalbehavior of BLM at Co/GC modified electrode has been studied by linear sweep and cyclicvoltammetry, The reaction of BLM with DNA formed an electrochemically nonactive complex, whichresulted in a decrease in the BLM equilibrium concentration and its reduction current. The decreasein peak current was proportional to DNA concentration and could be used to determination DNAconcentration. The equilibrium constant of DNA-BLM complex β was determined to be 5.16 x 10^(16).The experiments of AES and XPS showed that Co was surely implanted into the surface of GCE( glassycarbon electrode) and the depth distribution of Co was in good agreement with Gaussian normaldistribution; the implanted Co at GCE improved the electrocatalytic activity.

微创人工耳蜗植入技术

近几年来微创人工耳蜗植入（minimally invasive cochlear implantation，MICI）在国内、外耳科学界不断被提出。微创手术（minimallyinvasivesurgery）这一治疗理念始于1985年，当时英国泌尿外科医师Payne Wickham在内镜治疗泌尿道结石的报道中首次使用“minimally invasiveprocedure”一词来描述治疗过程，随后在医学各个学科领域内被广泛使用，微创手术这一理念也被广泛传播。

声电联合刺激和软电极植入

1声电联合刺激出现背景人工耳蜗植入（cochlearimplantation，CI）手术的研究方向应该与植入者更高的要求一致。世界上有超过一半的语言显著依赖音调，精细听力的缺乏降低了语言的感知能力，植入者无法获得精细听力，如对音调的细微感知能力，便无法同常人一样去欣赏音乐。