Comparison on Four Extraction Methods of Genomic DNA from Clematis fasciculiflora Franch

[Objective] This study aimed at comparing the four extraction methods of genomic DNA from Clematis fasciculiflora Franch and determining the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch.[Method] Leavies of Clematis fasciculiflora Franch were used as materials for comparing the purity and concentration of extracted DNA and extracting time among the four extraction methods of genomic DNA including improved CTAB method Ⅰ,improved CTAB method Ⅱ,improved CTAB method Ⅲ and improved SDS method.[Result] The four extraction methods could all be successfully used for extracting the genomic DNA from Clematis fasciculiflora Franch.The purity of genomic DNA was the highest using improved CTAB method Ⅰ,with the longest extracting time;while the concentration of genomic DNA was the maximum using the improved SDS method,with the shortest extracting time and relatively low purity;the extracting time of improved CTAB method Ⅲ was the shortest.[Conclusion] This study had established the optimal extraction method for extracting the genomic DNA from Clematis fasciculiflora Franch and supported for the further research using molecular biological methods.

A spatiotemporal evolution model of a short-circuit arc to a secondary arc based on the improved charge simulation method

The initial shape of the secondary arc considerably influences its subsequent shape.To establish the model for the arcing time of the secondary arc and modify the single-phase reclosing sequence,theoretical and experimental analysis of the evolution process of the short-circuit arc to the secondary arc is critical.In this study,an improved charge simulation method was used to develop the internal-space electric-field model of the short-circuit arc.The intensity of the electric field was used as an independent variable to describe the initial shape of the secondary arc.A secondary arc evolution model was developed based on this model.Moreover,the accuracy of the model was evaluated by comparison with physical experimental results.When the secondary arc current increased,the arcing time and dispersion increased.There is an overall trend of increasing arc length with increasing arcing time.Nevertheless,there is a reduction in arc length during arc ignition due to short circuits between the arc columns.Furthermore,the arcing time decreased in the range of 0°-90°as the angle between the wind direction and the x-axis increased.This work investigated the method by which short-circuit arcs evolve into secondary arcs.The results can be used to develop the secondary arc evolution model and to provide both a technical and theoretical basis for secondary arc suppression.

Dynamic Characteristics of Functionally Graded Timoshenko Beams by Improved Differential Quadrature Method

This study proposes an effective method to enhance the accuracy of the Differential Quadrature Method(DQM)for calculating the dynamic characteristics of functionally graded beams by improving the form of discrete node distribution.Firstly,based on the first-order shear deformation theory,the governing equation of free vibration of a functionally graded beam is transformed into the eigenvalue problem of ordinary differential equations with respect to beam axial displacement,transverse displacement,and cross-sectional rotation angle by considering the effects of shear deformation and rotational inertia of the beam cross-section.Then,ignoring the shear deformation of the beam section and only considering the effect of the rotational inertia of the section,the governing equation of the beam is transformed into the eigenvalue problem of ordinary differential equations with respect to beam transverse displacement.Based on the differential quadrature method theory,the eigenvalue problem of ordinary differential equations is transformed into the eigenvalue problem of standard generalized algebraic equations.Finally,the first several natural frequencies of the beam can be calculated.The feasibility and accuracy of the improved DQM are verified using the finite element method(FEM)and combined with the results of relevant literature.

Calculation and Analysis of TVMS Considering Profile Shifts and Surface Wear Evolution Process of Spur Gear

Profile shift is a highly effective technique for optimizing the performance of spur gear transmission systems.However,tooth surface wear is inevitable during gear meshing due to inadequate lubrication and long-term operation.Both profile shift and tooth surface wear(TSW)can impact the meshing characteristics by altering the involute tooth profile.In this study,a tooth stiffness model of spur gears that incorporates profile shift,TSW,tooth deformation,tooth contact deformation,fillet-foundation deformation,and gear body structure coupling is established.This model efficiently and accurately determines the time-varying mesh stiffness(TVMS).Additionally,an improved wear depth prediction method for spur gears is developed,which takes into consideration the mutually prime teeth numbers and more accurately reflects actual gear meshing conditions.Results show that consideration of the mutual prime of teeth numbers will have a certain impact on the TSW process.Furthermore,the finite element method(FEM)is employed to accurately verify the values of TVMS and load sharing ratio(LSR)of profile-shifted gears and worn gears.This study quantitatively analyzes the effect of profile shift on the surface wear process,which suggests that gear profile shift can partially alleviate the negative effects of TSW.The contribution of this study provides valuable insights into the design and maintenance of spur gear systems.

Dynamics and Exact Solutions of (1 + 1)-Dimensional Generalized Boussinesq Equation with Time-Space Dispersion Term

We study exact solutions to (1 + 1)-dimensional generalized Boussinesq equation with time-space dispersion term by making use of improved sub-equation method, and analyse the dynamical behavior and exact solutions of the sub-equation after constructing the nonlinear transformation and constraint conditions. Accordingly, we obtain twenty families of exact solutions such as analytical and singular solitons and singular periodic waves. In addition, we discuss the impact of system parameters on wave propagation.

Hybrid Nanofluid Flow over a Stretching Curved Surface with Induced Magnetic Field and Homogeneous-Heterogeneous Reactions

This study explores the 2D stretching flow of a hybrid nanofluid over a curved surface influenced by a magnetic field and reactions. A steady laminar flow model is created with curvilinear coordinates, considering thermal radiation, suction, and magnetic boundary conditions. The nanofluid is made of water with copper and MWCNTs as nanoparticles. The equations are transformed into nonlinear ODEs and solved numerically. The model’s accuracy is confirmed by comparing it with published data. Results show that fluid velocity increases, temperature decreases, and concentration increases with the curvature radius parameter. The hybrid nanofluid is more sensitive to magnetic field changes in velocity, while the nanofluid is more sensitive to magnetic boundary coefficient changes. These insights can optimize heat and mass transfer in industrial processes like chemical reactors and wastewater treatment.

Distance-Coefficient-Based Imaging Accuracy Improving Method Based on the Lamb Wave

An imaging accuracy improving method is established, within which a distance coefficient including location information between sparse array configuration and the location of defect is proposed to select higher signal- to-noise ratio data from all experimental data and then to use these selected data for elliptical imaging. Tile relationships among imaging accuracy, distance coefficient and residual direct wave are investigated, and then the residual direct wave is introduced to make the engineering application more convenient. The effectiveness of the proposed method is evaluated experimentally by sparse transducer array of a rectangle, and the results reveal that selecting experimental data of smaller distance coefficient can effectively improve imaging accuracy. Moreover, the direct wave difference increases with the decrease of the distance coefficient, which implies that the imaging accuracy can be effectively improved by using the experimental data of the larger direct wave difference.

Two New Identification Methods for Encephalitozoon cuniculi on Tissue Section

[ Objective] The paper aimed to search new identification methods of Encephalitozoon cuniculi on tissue sections. [ Method] Using improved Gram staining method and methyl green pyronin staining method, the pathological sections of sick rabbits were stained and identified. [ Result] The pathological changes in brain tissue could be clearly observed on sections, but parasites were not examined in pathological brain tissues stained by common staining method. When the pathological section was stained by improved Gram staining method, the pathological changes in brain tissue were not ouly stained very clearly, but blue parasites were also found in brain tissues. The parasites in epithelioid cells were stained into purple ones by methyl green pyronin staining method. [ Conclusion] The im- proved Gram staining method and methyl green pyronin staining method performed good staining effects of E. cuniculi in pathological sections, which were conducive to rapid diagnosis of encephalitozoonosis in rabbit.

Research on the Effective Ways of Improving the Teaching System of Physical Education Theory in Universities

Through the methods of literature and on-the-spot visit, this paper aims at studying the teaching system of physical education theory and the innovation of teaching methods in colleges and universities, and promoting the perfection of the teaching system of physical education （PE） theory in universities.

Water Quality Analysis of Fuping Section of the Shichuan River Based on Different Evaluation Methods

In order to study the water quality of the Shichuan River basin in Fuping,Shaanxi Province,based on improved Nemerow index method,comprehensive pollution index method and principal component analysis method,eight water quality indexes such as pH,dissolved oxygen(DO),total dissolved solids(TDS),COD,total hardness,total phosphorus,total nitrogen and Zn in three monitoring sections of Fuping section of the Shichuan River in Shaanxi Province were detected and analyzed.The results show that the water quality of the surface water in the Shichuan River basin is gradeⅢorⅣwater,that is,the water is slightly polluted and moderately polluted.It is necessary to monitor the water quality after regulation and clarify the main factors causing the water pollution.

Comparative Study on Results of Euler,Improved Euler and Run­ge-Kutta Methods for Solving the Engineering Unknown Problems

The paper presents the comparative study on numerical methods of Euler method,Improved Euler method and fourth-order Runge-Kutta method for solving the engineering problems and applications.The three proposed methods are quite efficient and practically well suited for solving the unknown engineering problems.This paper aims to enhance the teaching and learning quality of teachers and students for various levels.At each point of the interval,the value of y is calculated and compared with its exact value at that point.The next interesting point is the observation of error from those methods.Error in the value of y is the difference between calculated and exact value.A mathematical equation which relates various functions with its derivatives is known as a differential equation.It is a popular field of mathematics because of its application to real-world problems.To calculate the exact values,the approximate values and the errors,the numerical tool such as MATLAB is appropriate for observing the results.This paper mainly concentrates on identifying the method which provides more accurate results.Then the analytical results and calculates their corresponding error were compared in details.The minimum error directly reflected to realize the best method from different numerical methods.According to the analyses from those three approaches,we observed that only the error is nominal for the fourth-order Runge-Kutta method.

Advances in Mn‑Based Electrode Materials for Aqueous Sodium‑Ion Batteries

Aqueous sodium-ion batteries have attracted extensive attention for large-scale energy storage applications,due to abundant sodium resources,low cost,intrinsic safety of aqueous electrolytes and eco-friendliness.The electrochemical performance of aqueous sodium-ion batteries is affected by the properties of electrode materials and electrolytes.Among various electrode materials,Mn-based electrode materials have attracted tremendous attention because of the abundance of Mn,low cost,nontoxicity,eco-friendliness and interesting electrochemical performance.Aqueous electrolytes having narrow electrochemical window also affect the electrochemical performance of Mn-based electrode materials.In this review,we introduce systematically Mn-based electrode materials for aqueous sodium-ion batteries from cathode and anode materials and offer a comprehensive overview about their recent development.These Mn-based materials include oxides,Prussian blue analogues and polyanion compounds.We summarize and discuss the composition,crystal structure,morphology and electrochemical properties of Mn-based electrode materials.The improvement methods based on electrolyte optimization,element doping or substitution,optimization of morphology and carbon modification are highlighted.The perspectives of Mn-based electrode materials for future studies are also provided.We believe this review is important and helpful to explore and apply Mn-based electrode materials in aqueous sodium-ion batteries.

The impacts of ambiguity in preparation of 80% sulfuric acid solution and shaking time control of calibration solution on the determination of transparent exopolymer particles

The quantification of transparent exopolymer particles(TEP) by colorimetric method is of large error and low repeatability,one major reason of which is related to the absence of clear definition and evaluation for part steps of the original method.It is obscure that the 80% sulfuric acid solution,acted as the extraction solution in the determination of TEP,is prepared based on a volume ratio or mass ratio.Furthermore,the change of solubility of recently available Gum Xanthan(GX) from the market means that the original protocol is no longer applicable,and the grinding of GX stock solution with a tissue grinder is replaced by shaking with a rotating shaker in the study to prevent the excessive dissolution of GX.We found that different preparation techniques could result in the varied concentrations of 80% H_(2)SO_(4).The duration of shaking during the preparation of standard solution significantly affected the slope of the calibration curve,which caused different correction results of TEP.The impacts of different extraction solution concentrations and shaking time of GX solution on the quantification of TEP were investigated based on the field sampling and laboratory analysis.The extraction capacities of H_(2)SO_(4) with different concentrations for Alcian Blue were distinct,but had limited effect on the final measuring result of TEP.The change of the standard curve slope came along with the variation of shaking time,which markedly altered the detection limit and calibration result,and the extended shaking time was in favor of the determination of low-concentration TEP.It was suggested that the extraction solution concentration,shaking time and filtration volume of standard solution are required to be well controlled and selected to obtain more accurate results for TEP with different concentrations.

Seasonal variability of the mixed layer depth determined using an improved maximum angle method in the Arctic basins

To investigate the spatiotemporal variations in the mixed layer depth(MLD)in the Arctic basins,a new criterion to determine the MLD,called the improved maximum angle method(IMAM),was developed.A total of 45123 potential density profiles collected using Ice-Tethered Profilers(ITPs)in the Arctic basins during 2005-2021 were used to demonstrate the method’s effectiveness.By comparing the results obtained by the fixed threshold method(FTM),percentage threshold method(PTM),and maximum gradient method(MGM)for profiles in the Canada Basin,Makarov Basin,and Eurasian Basin,we determined that the quality index(1.0 for perfect identification of the MLD)of the IMAM regarding the assessment of the MLD determination method reached 0.94,which is much greater than those of other criteria.Moreover,two types of the density profiles were identified based on the mixed layer development stage.The MLDs of the typical profiles determined using the IMAM were found to have better consistency with the original definition.By utilizing the new mixed layer criterion,the seasonal variations and regional differences in the MLD in the Arctic basins were analyzed.Spatially,the summer and winter MLDs in the Canada Basin were the shallowest(13.55 m in summer,26.76 m in winter)than those in the Makarov(29.51 m in summer,49.08 m in winter)and Eurasian(20.36 m in summer,46.81 m in winter)basins due to the stable stratification in the upper ocean and the subsequent small effects of dynamic and thermodynamic processes(wind-driven stirring and brine rejection)in the Canada Basin.Seasonally,in the three Arctic basins,the average MLD was shallowest(22.77 m)in summer;it deepened through autumn and reached a winter maximum(41.12 m).

A Numerical Study of Several Species Population Models

This work considers a special case of Lotka-Volterra equations, which means that in the system of two ordinary differential equations, we take the four parameters equal to one. The reason is that we want just to illustrate the procedure to reduce that system to only one ordinary differential equation, such that we know its analytical solution. This idea will be applied to study the relations between a system of three ordinary differential equations, and a couple of partial differential equations. Lotka-Volterra equations are solved numerically by a fourth-order predictor-corrector method, which is initialized by an improved Euler method with a rather small time step because it is only a second-order algorithm. Then, it is proposed a model with three species, defined by ordinary differential equations.

Interval Q inversion based on zero-offset VSP data and applications

In order to obtain stable interval Q factor, by analyzing the spectrum of monitoring wavelet and down-going wavelet of zero-offset VSP data and referring the spectrum expression of Ricker wavelet, we propose a new expression of source wavelet spectrum. Basing on the new expression, we present improved amplitude spectral fitting and spectral ratio methods for interval Q inversion based on zero-offset VSP data, and the sequence for processing the zero-offset VSP data. Subsequently, we apply the proposed methods to real zero-offset VSP data, and carry out prestack inverse Q filtering to zero-offset VSP data and surface seismic data for amplitude compensation with the estimated Q value.

Isolation of the Flanking Sequences Adjacent to Transgenic T-DNA in Brassica napus Genome by an Improved Inverse PCR Method

[Objective] The research aimed to isolate flanking sequences adjacent to the transgenic T-DNA in Brassica napus by an improved inverse PCR method.[Method] Using single clone of transgenic FS4 in Brassica napus as the research materials,total DNA was extracted from transgenic Brassica napus by using modified CTAB method.After enzyme digestion and purification,self-joining was made.Two circles of nested PCR and the sequence alignment were carried out.[Result] A fragement with the size of 4.0 kb was amplified ...

Nonlinear principal resonance of magneto-electro-elastic thin plate

Considering magneto-electro-elastic thin plate, the von Karman plate theory of large deflection and the geometric nonlinearity, the mathematical model of nonlinear undamped forced vibration is established. Making use of the improved Lindstedt-Poincare （L-P） method, the undamped forced vibration problem is solved, and the amplitude-frequency response equation of thin plate is obtained. Furthermore, the amplitude frequency response curves of system under different condi- tions are obtained by numerical simulation. The results show that the thickness of the plate, mechanical excitation, parame- ter e, pure piezoelectric material of BaTiO3, pure piezomagnetic material of CoFe2 04, different magneto-electro-elastic ma- terials of BaTiO3/CoFe2 04 and Terfenol-D/PZT will have an impact on the system frequency response. The main effects in- volve principal resonance interval, spring stiffness characteristic and amplitude jumping phenomena.

An Improved Method for DNA Extraction from the Faeces of Red Deer

[Objective] To introduce an improved method for DNA extraction from the faeces of red deer. [Method] Based on the traditional method of CTAB lysis, we proposed an improved DNA extraction method according to the characteristics of red deer faeces. [Result] This improved method extracted high-quality fecal DNA from Tianshan red deer and amplified the mitochondrial cytochrome b gene. With the muscle and fur DNA of red deer as the control, the sequencing results further con- firmed the reliability of the method. [Conclusion] The method requires no proteinase K in the process of extraction, and the extracted DNA can be used for PCR ampli- fication directly without the purification of DNA purification kit, thus, it is cost-saving.