[Objectives]To explore the conditions for in vitro culture of Yangmei pollen.[Methods]Experiments were conducted on the germination characteristics of three types of Yangmei pollen using in vitro culture and germinati...[Objectives]To explore the conditions for in vitro culture of Yangmei pollen.[Methods]Experiments were conducted on the germination characteristics of three types of Yangmei pollen using in vitro culture and germination method.[Results]The suitable medium ratio for the germination of Yangmei pollen was 10%sucrose+0.01%borax+1%agar;the cultivation temperature of 30℃was more suitable for the germination of Yangmei pollen than 25℃;through analysis of variance,among the three types of Yangmei pollen,pollen of male 1 had the strongest viability,and it was the better pollination type.[Conclusions]The research could provide certain basis for introduction and cultivation of Yangmei and improving hybrid breeding effect.展开更多
Factors affecting the in vitro germination and growth of Taxus cuspidata embryos were examined. DCR medium was the best among 6 basal media tested; embryos from stage II seeds were the optimal developmental stage for ...Factors affecting the in vitro germination and growth of Taxus cuspidata embryos were examined. DCR medium was the best among 6 basal media tested; embryos from stage II seeds were the optimal developmental stage for in vitro germination; as the seeds approached maturity, germinability of embryos decrease. Embryos cultured in darkness tended to form callus and decrease the frequency of germination; inoculation method obviously affected frequency of embryo germination. Embryos cultured in optimal conditions developed into seedlings in 2 months.展开更多
文摘[Objectives]To explore the conditions for in vitro culture of Yangmei pollen.[Methods]Experiments were conducted on the germination characteristics of three types of Yangmei pollen using in vitro culture and germination method.[Results]The suitable medium ratio for the germination of Yangmei pollen was 10%sucrose+0.01%borax+1%agar;the cultivation temperature of 30℃was more suitable for the germination of Yangmei pollen than 25℃;through analysis of variance,among the three types of Yangmei pollen,pollen of male 1 had the strongest viability,and it was the better pollination type.[Conclusions]The research could provide certain basis for introduction and cultivation of Yangmei and improving hybrid breeding effect.
文摘Factors affecting the in vitro germination and growth of Taxus cuspidata embryos were examined. DCR medium was the best among 6 basal media tested; embryos from stage II seeds were the optimal developmental stage for in vitro germination; as the seeds approached maturity, germinability of embryos decrease. Embryos cultured in darkness tended to form callus and decrease the frequency of germination; inoculation method obviously affected frequency of embryo germination. Embryos cultured in optimal conditions developed into seedlings in 2 months.
