多房棘球蚴囊泡来源EVs及其emu-miR-10-5p促进小鼠肝星状细胞活化

目的明确多房棘球绦虫囊泡来源胞外囊泡(Extracellular vesicles,EVs)及其emu-miR-10-5p在体外调节HSCs活化中的作用。方法小鼠腹腔注射原头节构建继发性多泡型包虫病模型,以小鼠体内病灶为材料培养多房棘球绦虫囊泡并进行PCR鉴定。透射电镜观察培养囊泡组织中胞外囊泡结构。差速-超速离心法分离囊泡培养上清中EVs,透射电镜、纳米粒径以及Western blot对分离EVs进行鉴定。CCK-8检测不同浓度EVs作用后HSCs活力。EVs作用于HSCs后RT-qPCR和Western blot检测α-SMA、CollagenⅠ和CollagenⅢ表达水平。生物信息学分析确定emu-miR-10-5p为特异性miRNA。过表达emu-miR-10-5p后EdU检测HSCs增殖,RT-qPCR个Western blot检测细胞中α-SMA、CollagenⅠ和CollagenⅢ表达水平。干扰囊泡组织中emu-miR-10-5p后提取培养上清液中EVs作用于HSCs后,检测HSCs细胞中α-SMA、CollagenⅠ和CollagenⅢ表达水平。结果成功通过小鼠体内病灶材料培养出多房棘球蚴囊泡,经PCR扩增特异性基因证实为多房棘球绦虫来源。TEM观察培养囊泡表面有较多的囊状结构。通过差速-超速离心分离出多房棘球蚴囊泡来源EVs,并经TEM证实为球形、膜状结构。囊泡来源EVs能被HSCs内化,并促进HSCs增殖和活化。通过综合分析多房棘球绦虫感染小鼠血清、棘球蚴组织、棘球蚴囊泡来源EVs、生发层细胞、原头节miRNA测序结果,发现emu-miR-10-5p在上述成分中均存在表达,并通过RT-qPCR证实emu-miR-10-5p在多房棘球蚴囊泡来源EVs中存在的量较高。进一步研究证实过表达emu-miR-10-5p能促进HSCs增殖,增加HSCs中α-SMA、Collagen I和CollagenⅢmRNA及蛋白表达水平。而干扰囊泡中emu-miR-10-5p后从培养上清液中提取的EVs未增加α-SMA、Collagen I和CollagenⅢmRNA及蛋白表达水平。结论多房棘球蚴囊泡来源EVs及emu-miR-10-5p能够促进HSCs活化。

Matlab-Simulink Simulation Model of Electric Multiple Unit(EMU)Series ŽS 413/417 in Traction and Electric Braking Mode

In the paper,the Matlab-Simulink model of simulation of operation of theŽS 413/417 series EMU(electric multiple unit)in traction and braking mode is exposed where changes are observed:stator currents of three-phase traction motors,traction electric motor speeds and EMU,electromagnetic torque on the rotor shaft of the traction electric motor and DC(direct current)bus voltage.The model allowed review of the listed parameters for:different allowed values of contact network voltage and total voltage distortion at the place of connection of the EMU to the contact network,different mechanical loads of EMU and traction electric motor and different train speeds and rotation of traction electric motors.Appropriate conclusions were made through the analysis of the simulation results obtained.

基于EMU8086的微机原理教学实践探索——以8086数据寻址方式为例

从微机原理课程特点及教学现状出发,分析传统教学方式的弊端。探讨了EMU8086在微机原理课程线上线下混合式教学模式中的应用,以8086的数据寻址方式的教学为例,介绍了EMU8086仿真软件的特点和功能,分析了8086数据寻址方式在该课程中的重要性。通过实际案例介绍如何使用EMU8086进行8086数据寻址方式的教学。实践表明,在混合式教学模式下,通过使用EMU8086辅助教学,学生可以更直观地理解8086处理器的工作原理和数据寻址方式,能够有效提高学生对微机原理的理解和实际操作能力。

Investigation on welding procedures for in-service pipelines

Some research results are given in this paper about burnthrough and hydrogen cracking with a flowing chamber and a loop. Many factors including plate thickness, running rate, heat input and so forth have been studied. By experiments it can be found that occurrence of hydrogen cracking can be effectively reduced by properly increasing heat input and using the tempering bead technique.

Study on the microstructure and hardness of in-service welded joint of X70 pipeline steel

Hydrogen induced cracking （HIC） is one of the main problems of in-service welding onto active pipeline. Microstructure and hardness of welded joint have a vital effect on hydrogen induced cracking. The microstructure and hardness of welded joint of XTO pipeline steel were studied using simulation in-service welding device. The results show that the main microstructures of in-service welded seam are grain boundary ferrite , intracrystalline acicular ferrite , as well as small amount of widmanztatten structure. The main microstructures of coarse grain heat-affected zone （CGHAZ） are coarse granular bainite, lath ferrite and martensite. Metastable phases such as martensite and lath ferrite are found in CGHAZ because of the too quick cooling velocity a＇nd the hardness of the CGHAZ is high.

FAILURE ANALYSIS AND INSPECTION OF CRACKING OF IN-SERVICE CATALYTIC REGENERATOR

The special subject 'research on life prediction technology of important in-service pressure' mainly analyzes the failure mechanism of large-sized important and criticalin-service pressure vessels under the action of working medium and investigates safety assessmentand life prediction technology with a view to enhance the operation reliability of in-servicepressure vessels in China. Based on a series of accident investigation and test & measuringresearch, the cause of cracking of catalytic regenerator is analyzed and the in-line non-destructiveexamination method and failure prevention measures for the cracking of catalytic regenerator areproposed.

Influence of cooling conditions on X70 pipeline steel in-service welding HAZ

The chamber device was designed and set up to simulate the in-service welding. The results show ： the t8/5 , t8/3 and inner wall peak temperature Tp decrease with the cooling rate increases. The welding energy is carried off by flowing medium, the cooling rate increases, and many unbalanced microstructures such as granular bainite, martensite and M-A generate ; it worsens the properties of HAZ. Under air-cooling, the cooling rate is slow, the austenite grain grows obviously, the lath ferrite crosses the whole austenite, and it causes the hardness value is also big. The change of HAZ width is not obvious with the increase of cooling rate; and burn-through is not susceptible to the cooling rate. The quench microstructures increase and the hydrogen does not outflow from the HAZ easily when increase the cooling rate, so the susceptibility of hydrogen cracking increases.

Numerical simulation of mechanical properties of straight pipe and elbow during in-service welding

Elbows are the most vulnerable parts in pipeline network systems. The residual stress for in-service welding repair has significant impacts on the mechanical properties of straight pipes and elbows. In this paper,the thermal elastic-plastic finite element method is employed to investigate the mechanical field during the in-service welding. The prediction of residual stress and deformation in the straight pipe and elbow is performed based on the validation of the numerical models. And the effects of the curvature radius and defects on the elbow are investigated. The results show that the residual stress distribution is uneven along various directions after welding. And the mechanical properties of the elbow decrease when the curvature radius is small. Compared to the intact elbow,the residual stress of the elbow with defects concentrates in the defective area. The depth of defect is the main factors affecting the mechanical properties of the elbow. A systematic analysis of the mechanical properties of straight pipes and elbows is proposed to provide guidance to the in-service welding.

Numerical simulation of stress and deformation of in-service welding onto gas pipeline

SYSWELD was used to simulate in-service welding process of gas pipeline of X70 pipeline steel. Welding thermal cycle, stress and deformation of in-service welded joint were studied. The results show that peak temperature of coarse grain heat-affected zone （CGHAZ） of in-service welding onto gas pipeline is the same with routine welding, but ts/5, ts/3 and ts/1 decrease at certain degree. For the zone near welded seam, axial stress and hoop stress in the inner pipe wall are compressive stress when welding source passes through the cross-section that is studied, but residual axial stress and residual hoop stress after welded are all tensile stress. Transient deformation and residual deformation are all convex deformation compared with the original pipe diameter size. Deformation achieves maximum when welding thermal source passes through the cross-section that is studied and then decreases during the cooling process after welding.

Influence of pipe parameters and heat input on thermal cycle of in-service welding onto active gas pipeline

The software of SYSWELD was used to build model and simulate thermal cycle of in-service welding onto active gas pipeline. Influence of pipe diameter, wall thickness and heat input on thermal cycle was studied. The results show that t8/5 , t8/3 and peak temperature of inner surface decrease when wall thickness increases from 5 mm to 12 mm. But t8/1 will increases with the increase of wall thickness and will decrease after the wall thickness is larger than 7 mm. Pipe diameter has little influence on thermal cycle and that influence can be ignored when pipe diameter is greater than 273 mm. t8/5 , t8/3 , t8/1 and peak temperature of inner surface will increase with the increase of heat input.

Effect of In-Service Training Program on the Practice of Healthcare Workers toward Malaria Prevention and Treatment Guidelines during Pregnancy in Health Facilities in Jowhar District, Somalia

Malaria is generally considered a major public health problem in Somalia. Providing early diagnosis and effective treatment is the key element of malaria control strategies in malaria-endemic countries, including Somalia. This required to advocate and ensure health worker’s adherence to the national malaria guidelines at all levels of health care service. A well-designed in-service training program may improve the level of health worker’s adherence to national malaria treatment guidelines, although results have been inconsistent. This is an interventional health facility-based pre and post comparative study aimed to assess the effect of an in-service training program on the practice of healthcare workers toward malaria prevention and treatment guidelines, during in pregnancy in health facilities in Jowhar district, Middle Shabelle region of Somalia. The study was implemented in three phases: pre-intervention phase, intervention phase and post-intervention phase. The sample size consisted of (n = 150) health workers who were selected from ten public health facilities using proportional to size sampling;the data collection adopted in this research is composed of a structured interview questionnaire and observational checklist. Data was analyzed through the application of descriptive statistical analysis that includes frequency and percentage and the Chi-square (x2) test was used to test the associations among variables using SPSS software version 25. The study showed that the level of health workers’ awareness of the national malaria guidelines in the treatment and prevention of malaria in pregnancy was found to be good before the intervention 89 (59.3%) and this proportion increased to 150 (100%) post-intervention of the training program. A significance difference has been observed between health workers’ awareness and their adherence to the malarial guidelines at pre-test and post-test with a p-value 0.000. The proportion of health workers who attended previous training on national malaria guidelines in the treatment and prevention of malaria in pregnancy increased from 46 (30.7%) at the pre-test to 150 (100%) after the post-test. A significant difference was observed in the training status among different categories of health worker and their adherence to the guidelines during the pre- and post-intervention of the training program, with a p-value of 0.000. The result showed that health workers were adhering to the guidelines at the pre-test 33 (22%), this increased after the post-test to 87 (58%). The knowledge of the need to adhere led to an increase in the adherence rate after the training program intervention. The study reveals that inadequate awareness was most reason for the non-adherence in the majority of the health workers as indicated by 89 (59.3%) at the pre-test and 56 (37.3%) in the post-test. However, difference was not significant between the availability of anti-malaria drugs in the facilities and the health workers’ adherence to the guidelines p-value 0.355 at the pretest and p-value 0.258 at post-test. The study concluded that the in-service training program significantly improved health workers’ knowledge and practice to the national malaria guidelines in the treatment, and prevention of malaria in pregnancy. The researcher recommends that the national malaria control programme (NMCP) of the Federal Ministry of Health should provide continuous regular in-service training to frontline healthcare workers at (facility and Community-based) to upgrade their skills and knowledge towards the malaria guidelines, disseminate job aids to the health facilities and undertake regular monitoring to ensure effective implementation of the national malaria treatment guidelines in the treatment and prevention of malaria in pregnancy in achieving desired proper case-management practices of malaria in pregnancy at all levels of health care service.

Investigation and Analysis of Continuing Nursing Education Status and Needs of In-Service Nurses in Tibet

Objective: To investigate and analyze the current status of continuing nursing education (CNE) of in-service nurses in Tibet and their demand for continuing education. Methods: 663 active nurses in Tibet were taken as the research objects, and the self-developed questionnaire was used to investigate the current status and needs of CNE, and the correlation between the CNE needs of active nurses and their personal conditions was analyzed. Results: This study shows that 65.61% (435/663) of active nurses have not participated in any CNE, and 91.86% (609/663) of active nurses believe that they need to participate in CNE. The problems faced by active nurses in participating in CNE include funding restrictions, time limitation and ability limitation. Logistic stepwise regression analysis reveals that the age of active nurses and working years of nurses are negatively correlated with the funding restrictions for participating in CNE (P < 0.05). It is relatively more difficult for young nurses with less working experience to obtain funding for participating in CNE. Moreover, there is a negative correlation between age, educational level and the ability of nurses to participate in CNE (P < 0.05). Younger nurses with lower educational level are more difficult to participate in CNE due to personal ability. There is a positive correlation between the length of working experience and the time limitation faced to participate in CNE (P < 0.05). Senior nurses are too busy with their work to participate in CNE due to limited time. Conclusion: The problems faced by active nurses in Tibet in participating in CNE come from funding, time and ability. Young nurses with limited working experience cannot participate in CNE resulting from limited ability and funds;while senior nurses are mainly restricted by time. It is recommended that hospitals and nursing management in Tibet should solve these problems faced by nurses in participating in CNE by implementing a flexible scheduling system, and providing funding or implementing an incentive system, to enhance the confidence of active nurses in participating in CNE.

In-service Structural Health Monitoring of a Full-scale Composite Horizontal Tail

In-service structural health monitoring（SHM） technologies are critical for the utilization of composite aircraft structures. We developed a Lamb wave-based in-service SHM technology using built-in piezoelectric actuator/sensor networks to monitor delamination extension in a full-scale composite horizontal tail. The in-service SHM technology combine of damage rapid monitoring（DRM） stage and damage imaging diagnosis（DID） stage allows for real-time monitoring and long term tracking of the structural integrity of composite aircraft structures. DRM stage using spearman rank correlation coeffi cient was introduced to generate a damage index which can be used to monitor the trend of damage extension. The DID stage based on canonical correlation analysis aimed at intuitively highlighting structural damage regions in two-dimensional images. The DRM and DID stages were trialed by an in-service SHM experiment of CFRP T-joint. Finally, the detection capability of the in-service SHM technology was verified in the SHM experiment of a full-scale composite horizontal tail. Experimental results show that the rapid monitoring method effectively monitors the damage occurrence and extension tendency in real time; damage imaging diagnosis results are consistent with those from the failure model of the composite horizontal tail structure.

An abnormal carbody swaying of intercity EMU train caused by low wheel-rail equivalent conicity and damping force unloading of yaw damper

Low-frequency carbody swaying phenomenon often occurs to railway vehicles due to hunting instability,which seriously deteriorates the ride comfort of passengers.This paper investigates low-frequency carbody swaying through experimental analysis and numerical simulation.In the tests,the carbody acceleration,the wheel-rail profiles,and the dynamic characteristics of dampers were measured to understand the characteristics of the abnormal carbody vibration and to find out its primary contributor.Linear and nonlinear numerical simulations on the mechanism and optimization measures were carried out to solve this carbody swaying issue.The results showed that the carbody swaying is the manifest of carbody hunting instability.The low equivalent conicity and the decrease of dynamic damping of the yaw damper are probably the cause of this phenomenon.The optimization measures to increase the equivalent conicity and dynamic damping of the yaw damper were put forward and verified by on-track tests.The results of this study could enrich the knowledge of carbody hunting and provide a reference for solving abnormal carbody vibrations.

Maintenance Optimization for EMU Trains Considering Environmental Impacts and Correlations

The complexity of the actual operating environment of EMU trains and the interaction between the reliability of system components have become a huge challenge for the maintenance scheduling of EMU trains. In response to these problems, the evolution of reliability and failure rate under the influence of environmental factors, failure correlations and economy correlations is analyzed. We assume bogie systems form the EMU train in series. The failure correlation matrix of the bogie systems is modeled. With the lowest total maintenance cost as the optimization objective, a decision-making model for EMU train maintenance is established. A dynamic maintenance strategy is proposed for the model, which can improve maintenance plans efficiently. Artificial bee colony algorithm is applied to further iteratively optimize the threshold parameters in the strategy. The results are calculated and verified by a numerical example. The results show the effectiveness of the maintenance decision model. The dynamic maintenance strategy in this paper is compared with the traditional opportunistic maintenance strategy. The proposed maintenance strategy outperforms the traditional opportunistic maintenance strategy in the numerical example.

轻量化动车组车载牵引变压器的研制

车载牵引变压器是动车组单体最重的电气设备,其轻量化有助于动车组节能降耗与运输效率提升。现有车载牵引变压器均为油浸式,干式变压器是其轻量化的主要方案,但目前干式变压器采用的环氧树脂绝缘易开裂、散热无法满足要求。为此,以具有良好力学性能的硅橡胶为基体材料,首先通过温升分析研究绝缘材料热导率需求,然后制备样品开展实验,以电学性能和力学性能为约束条件研究绝缘材料导热性能改进方法,进而研究车载牵引变压器轻量化设计方法,最后依据研制的绝缘材料与轻量化设计方法,设计并研制了一台容量为250 kVA的小型轻量化车载牵引变压器样机。结果表明:制备的高导热硅橡胶复合绝缘材料性能满足干式车载变压器对绝缘和力学性能的要求;研制的小型变压器样机质量为780 kg,相比同容量油浸式车载牵引变压器质量减少了46.2%,经试验测试得在额定条件下样机的热点温度值为167.68℃,满足车载牵引变压器运行需求。

底线思维与科技审度:高风险社会治理的要义与进路

当前AI大爆发及其应对乏力表明,西方科技发展模式的缺陷与人类社会的脆弱及学界研究的薄弱,更加凸显人类风险防控机制存在诸多漏洞及其引发的科技危机与人类安全危机,已把人类带入高风险社会。用底线思维与刘大椿提出的科技审度予以破解:底线思维解决非共识下的议而不决;科技审度和审度思维所特有的执两用中、包容互补、多元开放、整合升级、共识决策等,可提供有效的治理对策。科技转型是治理科技风险的根本解决之道,科技审度可发挥重要的启发与引导作用,以构建真善美的新型科技发展模式,加快世界科学中心建设,据此总结出高风险社会治理的要义与进路,以作为科技风险治理的研究指南。

车端侧滚减振装置对高速双层动车组动力学性能的影响分析

双层动车组设置较大的二系横向阻尼来抑制一次蛇行引起的共振,而较大的二系横向阻尼会使车辆的横向平稳性变差。针对某型高速双层动车组横向平稳性差的问题,基于车端侧滚减振装置的工作原理建立了车端侧滚减振装置在车体滚摆和摇头运动状态下的数学模型,并将AMEsim软件中建立的减振器仿真模型与台架试验进行验证。最后通过建立车端侧滚减振装置与被试车辆的联合仿真模型,在不改变车辆现有悬挂参数的基础上对车端侧滚减振装置的关键参数进行优化选取。仿真结果表明,车端侧滚减振装置可以在不影响车辆垂向平稳性、保证曲线运行安全性的前提下有效改善双层动车组的横向平稳性。

基于机器学习的长编重联动车组碰撞能量管理方案优化

为有效缓解交通运输压力,采用动车组重联运行可以成倍提高载客量,然而一旦发生碰撞事故,巨大的碰撞能量将造成严重的乘员损伤和财产损失,长编重联动车组碰撞能量管理已成为重点研究对象。本文提出均匀耗散和集中耗散2种碰撞能量管理模式,以头车和中间车车端的吸能装置平台力和压缩行程为设计参数,基于KNN、MLS、RBF和RF的4种机器学习算法,开展长编重联动车组碰撞能量管理方案优化设计。研究结果表明:预测头车吸能量和中间车自身耗散能量方差的最优机器学习模型分别是MLS和RBF,相对误差均在4%以内;头车和中间车的吸能元件平台力是影响头车界面吸能量的主要参数,中间车吸能装置参数是影响中间车界面碰撞能量分布是否均匀的主要参数;集中耗散模式下头车和重联界面吸收了碰撞能量48.24%,中间车界面吸收了碰撞能量51.76%,该能量分配模式要求头车前端吸能装置具有更高的吸能量;均匀耗散模式下头车和重联界面吸收了22.75%的碰撞能量,中间车界面吸收了77.25%的碰撞能量,该能量分配模式会增大车间距导致列车长度增加;优化获得的2种碰撞能量管理方案都能在时速36 km/h对撞工况下保证长编重联动车组车体结构完整,且车体120 ms最大平均加速度分别为2.64g和2.36g。

高速动车组车体整备垂弯频率优化研究

提出采用2种不同方法对高速动车组进行模态分析研究。以车体各个部件厚度参数为设计变量进行模态及质量的灵敏度分析,研究不同部件厚度对车体一阶垂弯频率的影响规律,并筛选8个部件作为最终设计变量,建立优化数学模型;以车体与车下主要设备连接处弹性元件的刚度参数为设计变量进行响应面分析,研究弹性元件刚度对车体一阶垂弯频率的影响规律,并对弹性元件刚度进行优化。结果表明:2种方法均取得了一定的效果,基于灵敏度分析的部件优化,使车体整备一阶垂弯频率提升了4.88%;基于响应面分析的弹性元件优化,使车体整备一阶垂弯频率提升了28.05%,大于相关标准中规定的10Hz,优化效果明显。