[Objective] The aim was to breed the indica-compatible japonica lines(ICJLs).[Method] The indica-compatibility of 5 candidate ICJLs were tested based on the pollen fertility and spikelet fertility of F1 hybrids with...[Objective] The aim was to breed the indica-compatible japonica lines(ICJLs).[Method] The indica-compatibility of 5 candidate ICJLs were tested based on the pollen fertility and spikelet fertility of F1 hybrids with 6 indica testers,6 japonica testers and 6 middle testers.[Result] Candidate ICJL G2123,G2417,G2410 and G3005 were grouped into the exceptionally(Specific)indica-compatible lines based on their high indica-compatibility and low japonica-compatibility.Candidate ICJL G2615 was grouped into the non-compatible lines on their low indica-compatibility and low japonica-compatibility.[Conclusion] The testers,fertility identification standards,test methods of pollen fertility and spikelet fertility were discussed when the compatibility of CICJLs was tested.The reason of the indica-compatibility in G2417 was analyzed.展开更多
[Objective] This study was conducted to screen japonica rice male sterile lines with good flowering characteristics under high temperature and summer drought. [Method] The flowering habits of 23 japonica male sterile ...[Objective] This study was conducted to screen japonica rice male sterile lines with good flowering characteristics under high temperature and summer drought. [Method] The flowering habits of 23 japonica male sterile lines, such as flowering peak, single-panicle flowering duration, single-plant flowering duration, en- closed glume rate, glume-opening angle, the distance between glumes, stigma exer- tion rate and sigma vitality, were compared with II-32A as a control under above weather condition. [Result] The flowering characteristics had significant difference a- mong the tested rice lines. Compared with the control, three japonica rice male ster- ile lines 35478A, 35489A and 35502A had better flowering traits, such as earlier flowering peak, longer single-panicle flowering duration, longer single-plant flowering duration, lower rate of enclosed glumes, larger glume angle and larger distance be- tween glumes. 35478A performed its flowering peak from 10:00-11:00 am, 1.5 h earlier than the control; 35489A exhibited higher stigma exertion rate, up to 76.23%; and 35502A had higher stigma vitality, up to 84.68%, which kept longer time than that of the CK. [Conclusion] The results will provide high-quality resources for the breeding of new japonica rice varieties with high-yield and high-temperature toler- ance.展开更多
Yangfujing 7A, derived from the cross between Xu 9201A and Yangfujing 7 and its successive backcrosses, is a BT-type japonica CMS line developed by the Agricultural Institute of Riparian Region of Jiangsu Province. It...Yangfujing 7A, derived from the cross between Xu 9201A and Yangfujing 7 and its successive backcrosses, is a BT-type japonica CMS line developed by the Agricultural Institute of Riparian Region of Jiangsu Province. It shows good integrat- ed characteristics, stable male sterility, good flowering habits, high out crossing rate, strong disease resistance, fine grain quality (reaching the 1st class of national standards for fine quality rice) and high combining ability. In 2012, it was technically identified in Jiangsu Province. Its F1 hybrid combination Tongyoujingl (Yangfujing 7 A/R98), showing high yield and good grain quality, was registered and released to commercial production by Jiangsu Crop Variety Appraisal Committee in 2013.展开更多
From the view of methodology for genetic improvement ofJaponicacytoplasmic male sterile(CMS) lines,we introduced the breeding process of the dwarf-abortive type(DA type)JaponicaCMS line Chunjiang 95A with wide compati...From the view of methodology for genetic improvement ofJaponicacytoplasmic male sterile(CMS) lines,we introduced the breeding process of the dwarf-abortive type(DA type)JaponicaCMS line Chunjiang 95A with wide compatibility,and further investigated the biological characteristics of the DA type Chunjiang 95A and its isonuclear alloplasmic CMS line Dian-I type Chunjiang 95A.The results show that DA type Chunjiang 95A is a japonicalinous CMS line and mainly typical abortive,which is better in fertility stability than stained abortive typeJaponica CMS lines.The DA type Chunjiang 95A is featured with high stigma exsertion,good flowering habit,high outcrossing rate and wide compatibility.Its recoverability differs from stained abortive typeJaponicaCMS lines.Moreover,we discussed genetic improvement of the typical abortive type JaponicaCMS lines and the application prospects of heterosis.展开更多
From 1986 to 1993, a set of near-isogenic japonicarice Iines with three major genes Xα-3, Xα - 4,and Xα-12 for resistance to bacterial blight(Xan-thomonas oryzae pv.oryzae)were developed anddesignated as CBB3, CBB4...From 1986 to 1993, a set of near-isogenic japonicarice Iines with three major genes Xα-3, Xα - 4,and Xα-12 for resistance to bacterial blight(Xan-thomonas oryzae pv.oryzae)were developed anddesignated as CBB3, CBB4, and CBB12 respective-展开更多
The Chaling wild abortive type (CL-type) cytoplasmic male sterile line, E-Jing A, was characterized by stable sterility with a sterile plant percentage of 100%, a pollen sterile degree of 98% with dominated typical ...The Chaling wild abortive type (CL-type) cytoplasmic male sterile line, E-Jing A, was characterized by stable sterility with a sterile plant percentage of 100%, a pollen sterile degree of 98% with dominated typical abortive pollens, and showed the characteristics of good outcrossing with a stigma exsertion rate of 48.2% and an outcrossing rate varied from 30% to 50%, good combining ability, and fine grain quality. E-Jing A was sensitive to temperature. Its duration from sowing to heading was 78-90 d when it was sown from mid May to early June at Wuhan. The breeding procedure of E-Jing A and its characteristics, relationship of restoring and maintaining were introduced.展开更多
Yannong S, a reverse thermo-sensitive genic male sterile (TGMS) line, is sterile at high temperature and fertile at low temperature. In the present study, the fertility of Yannong S and the japonica reverse TGMS lin...Yannong S, a reverse thermo-sensitive genic male sterile (TGMS) line, is sterile at high temperature and fertile at low temperature. In the present study, the fertility of Yannong S and the japonica reverse TGMS lines derived from it was in- vestigated in Harbin, Heilongjiang from 2013 to 2015. The results showed that Yan- nong S was completely male sterile in July and August (the hottest months of the year in Harbin), and the seed setting rate of bagged panicles was 0. We also in- ferred that the critical temperature for inducing the sterility of Yannong S was higher than 28 ℃. Most of the japonica reverse TGMS lines derived from Yannong S were completely aborted in July and August, with a seed setting rate of 0. It can be con- cluded that the reverse TGMS lines derived from Yannong S are genetically stable lines that have a long period of sterility in cold regions, so they may be the ideal genetic materials for the development and in-situ seed production of japonica hybrid rice.展开更多
Unconditional and conditional QTL mapping were conducted for growth duration (GD), plant height (PH) and effective panicle number per plant (PN) using a recombinant inbred line (RIL) population derived from a cross be...Unconditional and conditional QTL mapping were conducted for growth duration (GD), plant height (PH) and effective panicle number per plant (PN) using a recombinant inbred line (RIL) population derived from a cross between two japonica rice varieties Xiushui 79 and C Bao. The RIL population consisted of 254 lines was planted in two environments, Nanjing and Sihong, Jiangsu Province, China. Results showed that additive effects were major in all of QTLs for GD, PH and PN detected by the two methods, and the epistatic effects explained a small proportion of phenotypic variation. No interactions were detected between additive QTL and environment, and between epistatic QTL pairs and environment. After growth duration was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.71. When effective panicle number per plant was adjusted to an identical level, RM448-240bp was detected as an applicable elite allele for GD, with an additive effect of 4.64. After plant height was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.62, and RM448-240bp was detected as an applicable elite allele for GD, with an additive effect of 3.89. These applicable elite alleles could be used to improve target traits without influencing the other two traits.展开更多
Genetic analysis of rolled leaf is important to rice ideotype breeding. To detect loci controlling rolled leaf of japonica restorer lines, SSR marker genotypes and phenotypes of flag leaf rolling index (LRI) were in...Genetic analysis of rolled leaf is important to rice ideotype breeding. To detect loci controlling rolled leaf of japonica restorer lines, SSR marker genotypes and phenotypes of flag leaf rolling index (LRI) were investigated in Xiushui 79 (P1, a japonica rice variety), C Bao (P2, a japonica restorer line) and 254 recombinant inbred lines derived from the cross between P1 and P2 , and in two environments. A genetic map of this cross was constructed, QTLs for LRI were detected and their interactions with environments were analyzed. Among 818 pairs of SSR primers, 90 primers showed polymorphism between P1 and P2, and 12 markers showed highly significant correlation with LRI in both environments based on single marker regression analysis. The genetic map containing 74 information loci has a total distance of 744.6 cM, with an average of 10.1 cM between two adjacent loci. Three QTLs (qRL-1, qRL-7 and qRL-8-1) were detected with two softwares: WinQTLCart 2.5 and QTLNetwork2.0. qRL-8-1 was a new locus, accounting for 15.5% and 12.8% of phenotypic variations in the two environments, respectively. The phenotypic variation explained by additive effect was 6.6%. No interaction was found between qRL-8-1 genotype and environments.展开更多
Development of the recombinant inbred line populations (RILs) is important basis to detect QTLs for cold tolerance at booting stage in rice. A set of 230 RILs derived from the cross of Towada and Kunmingxiaobaigu we...Development of the recombinant inbred line populations (RILs) is important basis to detect QTLs for cold tolerance at booting stage in rice. A set of 230 RILs derived from the cross of Towada and Kunmingxiaobaigu were used for evaluation of low-temperature response on major agronomic traits of plant height (PH), panicle length (PL), panicle exsertion (PE), spikelet fertility (SF), specific spikelet fertility (SSF), and spikelets per panicle (SPP) under natural low-temperature growing environments in Yunnan Province, China. The results showed PH, PE, and SPP were mainly attributed by genotypes. PL was mainly influenced interactively by the genotypes × environments. SF and SSF were mainly controlled by the environments. Under the five different growth environments, F values of the six agronomic traits mentioned above ranged from 4.019 to 97.284. Significant difference was revealed between the lines. Under every environment, it indicated significantly positive correlation between SF and SSF, with correlation coefficients ranged from 0.826 to 0.885. It indicated significantly positive correlation between PH, PL, and PE. Under five different growing environments, variation coefficients of the six characters ordered in SSF (66.3%) 〉 PE (57.4%) 〉 SP (37.2%) 〉 SPP (16.2%) 〉 PH (9.6%) 〉 PL (6.4%). SSF, PE and SF were most sensitive to low temperature stress at booting stage, while SPP, PH and PL being least. The RILs of Towada/ Kunmingxiaobaigu can be used as a genetic population to investigate cold tolerance at booting stage. SSF, PE and SF are most sensitive to cold tolerance at booting stage in rice. So far the the variation of PH, PL, and SPP related to cold tolerance are not clear under natural low-temperature environment. More tested environments and years are required to identify and evaluate cold tolerance at booting stage in rice.展开更多
indica and japonica are the two most important subspecies of Asian cultivated rice. Identifying mechanisms responsible for population differentiation in these subspecies is important for indica-japonica hybridization ...indica and japonica are the two most important subspecies of Asian cultivated rice. Identifying mechanisms responsible for population differentiation in these subspecies is important for indica-japonica hybridization breeding. In this study, subspecies and economic trait differentiation patterns were analyzed using morphological and molecular (InDel and Intron Length Polymorphism) data in F2 and F3 populations derived from indica-japonica hybridization. Populations were grown in Liaoning and Guangdong provinces, China, with F3 populations generated from F2 populations using bulk harvesting (BM) and single-seed descent methods (SSD). Segregation distortion was detected in F3-BM populations, but not in F3- SSD or in F2 populations. Superior performance was observed with respect to economic traits in Liaoning compared with that in Guangdong and 1 000-grain weight (KW), seed setting rate (SSR) and grain yield per plant (GYP) were significantly correlated with indica and japonica subspecies types. Analysis of molecular and morphological data demonstrated that the environment is the main factor giving rise to population differentiation in indica-japonica hybridization. In addition, we also found that KW, SSR and GYP are related to subspecies characteristics and kinship, which is possibly a significant factor resulting in economic trait differentiation and determining environmental adaptability. Our study has provided new insights into the process of population differentiation in these subspecies to inform indica-japonica hybridization breeding.展开更多
基金Supported by National High Technology Development Plan Financial Program(101-Z16-02-01,2001AA211181)~~
文摘[Objective] The aim was to breed the indica-compatible japonica lines(ICJLs).[Method] The indica-compatibility of 5 candidate ICJLs were tested based on the pollen fertility and spikelet fertility of F1 hybrids with 6 indica testers,6 japonica testers and 6 middle testers.[Result] Candidate ICJL G2123,G2417,G2410 and G3005 were grouped into the exceptionally(Specific)indica-compatible lines based on their high indica-compatibility and low japonica-compatibility.Candidate ICJL G2615 was grouped into the non-compatible lines on their low indica-compatibility and low japonica-compatibility.[Conclusion] The testers,fertility identification standards,test methods of pollen fertility and spikelet fertility were discussed when the compatibility of CICJLs was tested.The reason of the indica-compatibility in G2417 was analyzed.
基金Supported by Scientific and Technological Talents Program of Chongqing(cstc2013kjrcqnrc80002)Key Science and Technology Research and Development Program of Chongqing(cstc2012gg B80005,cstc2012gg C80002)the Fund from Chongqing Academy of Agricultural Sciences for Agricultural Development(NKY,2013AC008)~~
文摘[Objective] This study was conducted to screen japonica rice male sterile lines with good flowering characteristics under high temperature and summer drought. [Method] The flowering habits of 23 japonica male sterile lines, such as flowering peak, single-panicle flowering duration, single-plant flowering duration, en- closed glume rate, glume-opening angle, the distance between glumes, stigma exer- tion rate and sigma vitality, were compared with II-32A as a control under above weather condition. [Result] The flowering characteristics had significant difference a- mong the tested rice lines. Compared with the control, three japonica rice male ster- ile lines 35478A, 35489A and 35502A had better flowering traits, such as earlier flowering peak, longer single-panicle flowering duration, longer single-plant flowering duration, lower rate of enclosed glumes, larger glume angle and larger distance be- tween glumes. 35478A performed its flowering peak from 10:00-11:00 am, 1.5 h earlier than the control; 35489A exhibited higher stigma exertion rate, up to 76.23%; and 35502A had higher stigma vitality, up to 84.68%, which kept longer time than that of the CK. [Conclusion] The results will provide high-quality resources for the breeding of new japonica rice varieties with high-yield and high-temperature toler- ance.
基金Supported by the Science and Technology Innovation Program of Nantong City,Jiangsu Province(HL2012019)~~
文摘Yangfujing 7A, derived from the cross between Xu 9201A and Yangfujing 7 and its successive backcrosses, is a BT-type japonica CMS line developed by the Agricultural Institute of Riparian Region of Jiangsu Province. It shows good integrat- ed characteristics, stable male sterility, good flowering habits, high out crossing rate, strong disease resistance, fine grain quality (reaching the 1st class of national standards for fine quality rice) and high combining ability. In 2012, it was technically identified in Jiangsu Province. Its F1 hybrid combination Tongyoujingl (Yangfujing 7 A/R98), showing high yield and good grain quality, was registered and released to commercial production by Jiangsu Crop Variety Appraisal Committee in 2013.
基金Supported by Key Research Project of Zhejiang Province(2008C22074)0406 Program of Zhejiang Province+2 种基金Fund for theConversion of Agricultural Science and Technology Achievement inZhejiang ProvinceBasic Operation Fund of China National Rice Re-search Institutethe Program of Breeding and Experimental Dem-onstration of Super Hybrid Rice in China from Ministry of Agriculture.
文摘From the view of methodology for genetic improvement ofJaponicacytoplasmic male sterile(CMS) lines,we introduced the breeding process of the dwarf-abortive type(DA type)JaponicaCMS line Chunjiang 95A with wide compatibility,and further investigated the biological characteristics of the DA type Chunjiang 95A and its isonuclear alloplasmic CMS line Dian-I type Chunjiang 95A.The results show that DA type Chunjiang 95A is a japonicalinous CMS line and mainly typical abortive,which is better in fertility stability than stained abortive typeJaponica CMS lines.The DA type Chunjiang 95A is featured with high stigma exsertion,good flowering habit,high outcrossing rate and wide compatibility.Its recoverability differs from stained abortive typeJaponicaCMS lines.Moreover,we discussed genetic improvement of the typical abortive type JaponicaCMS lines and the application prospects of heterosis.
文摘From 1986 to 1993, a set of near-isogenic japonicarice Iines with three major genes Xα-3, Xα - 4,and Xα-12 for resistance to bacterial blight(Xan-thomonas oryzae pv.oryzae)were developed anddesignated as CBB3, CBB4, and CBB12 respective-
基金Supported by the Great Special Science and Technology Project of Hubei Province during the Eleventh Five-Year Plan(2006AA201A03)~~
文摘The Chaling wild abortive type (CL-type) cytoplasmic male sterile line, E-Jing A, was characterized by stable sterility with a sterile plant percentage of 100%, a pollen sterile degree of 98% with dominated typical abortive pollens, and showed the characteristics of good outcrossing with a stigma exsertion rate of 48.2% and an outcrossing rate varied from 30% to 50%, good combining ability, and fine grain quality. E-Jing A was sensitive to temperature. Its duration from sowing to heading was 78-90 d when it was sown from mid May to early June at Wuhan. The breeding procedure of E-Jing A and its characteristics, relationship of restoring and maintaining were introduced.
基金Supported by the Open-end Fund of State Key Laboratory of Hybrid Rice(2014KF05)the Scientific Research Program of Heilongjiang Farms and Land Reclamation Administration+1 种基金National Key Technology Research and Development Program(2014BAD01B03-02-05)Special Fund for Agro-scientific Research in the Public Interest(201403002-2-3)~~
文摘Yannong S, a reverse thermo-sensitive genic male sterile (TGMS) line, is sterile at high temperature and fertile at low temperature. In the present study, the fertility of Yannong S and the japonica reverse TGMS lines derived from it was in- vestigated in Harbin, Heilongjiang from 2013 to 2015. The results showed that Yan- nong S was completely male sterile in July and August (the hottest months of the year in Harbin), and the seed setting rate of bagged panicles was 0. We also in- ferred that the critical temperature for inducing the sterility of Yannong S was higher than 28 ℃. Most of the japonica reverse TGMS lines derived from Yannong S were completely aborted in July and August, with a seed setting rate of 0. It can be con- cluded that the reverse TGMS lines derived from Yannong S are genetically stable lines that have a long period of sterility in cold regions, so they may be the ideal genetic materials for the development and in-situ seed production of japonica hybrid rice.
基金supported by the Program of National High Technology Research and Development, Ministry of Science and Technology, China (Grant No. 2010AA101301)the Program of Introducing Talents of Discipline to University in China (Grant No. B08025)+1 种基金the Program of Introducing International Advanced Agricultural Science and Technology in China (Grant No. 2006-G8 [4]-31-1) the Program of Science-Technology Basis and Conditional Platform in China (Grant No. 505005)
文摘Unconditional and conditional QTL mapping were conducted for growth duration (GD), plant height (PH) and effective panicle number per plant (PN) using a recombinant inbred line (RIL) population derived from a cross between two japonica rice varieties Xiushui 79 and C Bao. The RIL population consisted of 254 lines was planted in two environments, Nanjing and Sihong, Jiangsu Province, China. Results showed that additive effects were major in all of QTLs for GD, PH and PN detected by the two methods, and the epistatic effects explained a small proportion of phenotypic variation. No interactions were detected between additive QTL and environment, and between epistatic QTL pairs and environment. After growth duration was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.71. When effective panicle number per plant was adjusted to an identical level, RM448-240bp was detected as an applicable elite allele for GD, with an additive effect of 4.64. After plant height was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.62, and RM448-240bp was detected as an applicable elite allele for GD, with an additive effect of 3.89. These applicable elite alleles could be used to improve target traits without influencing the other two traits.
基金supported by the Program of Introducing Talents of Discipline to University in China (Grant No.B08025)
文摘Genetic analysis of rolled leaf is important to rice ideotype breeding. To detect loci controlling rolled leaf of japonica restorer lines, SSR marker genotypes and phenotypes of flag leaf rolling index (LRI) were investigated in Xiushui 79 (P1, a japonica rice variety), C Bao (P2, a japonica restorer line) and 254 recombinant inbred lines derived from the cross between P1 and P2 , and in two environments. A genetic map of this cross was constructed, QTLs for LRI were detected and their interactions with environments were analyzed. Among 818 pairs of SSR primers, 90 primers showed polymorphism between P1 and P2, and 12 markers showed highly significant correlation with LRI in both environments based on single marker regression analysis. The genetic map containing 74 information loci has a total distance of 744.6 cM, with an average of 10.1 cM between two adjacent loci. Three QTLs (qRL-1, qRL-7 and qRL-8-1) were detected with two softwares: WinQTLCart 2.5 and QTLNetwork2.0. qRL-8-1 was a new locus, accounting for 15.5% and 12.8% of phenotypic variations in the two environments, respectively. The phenotypic variation explained by additive effect was 6.6%. No interaction was found between qRL-8-1 genotype and environments.
基金supported by the National Natural Science Foundation of China (30460065)the National 948 Key Program of Ministry of Agriculture of China (2006-G1)the National Key Technology R&D Program during the 11th Five-Year Plan period of China (2006BAD13B01)
文摘Development of the recombinant inbred line populations (RILs) is important basis to detect QTLs for cold tolerance at booting stage in rice. A set of 230 RILs derived from the cross of Towada and Kunmingxiaobaigu were used for evaluation of low-temperature response on major agronomic traits of plant height (PH), panicle length (PL), panicle exsertion (PE), spikelet fertility (SF), specific spikelet fertility (SSF), and spikelets per panicle (SPP) under natural low-temperature growing environments in Yunnan Province, China. The results showed PH, PE, and SPP were mainly attributed by genotypes. PL was mainly influenced interactively by the genotypes × environments. SF and SSF were mainly controlled by the environments. Under the five different growth environments, F values of the six agronomic traits mentioned above ranged from 4.019 to 97.284. Significant difference was revealed between the lines. Under every environment, it indicated significantly positive correlation between SF and SSF, with correlation coefficients ranged from 0.826 to 0.885. It indicated significantly positive correlation between PH, PL, and PE. Under five different growing environments, variation coefficients of the six characters ordered in SSF (66.3%) 〉 PE (57.4%) 〉 SP (37.2%) 〉 SPP (16.2%) 〉 PH (9.6%) 〉 PL (6.4%). SSF, PE and SF were most sensitive to low temperature stress at booting stage, while SPP, PH and PL being least. The RILs of Towada/ Kunmingxiaobaigu can be used as a genetic population to investigate cold tolerance at booting stage. SSF, PE and SF are most sensitive to cold tolerance at booting stage in rice. So far the the variation of PH, PL, and SPP related to cold tolerance are not clear under natural low-temperature environment. More tested environments and years are required to identify and evaluate cold tolerance at booting stage in rice.
基金supported by the National Natural Science Foundation of China (30971845)
文摘indica and japonica are the two most important subspecies of Asian cultivated rice. Identifying mechanisms responsible for population differentiation in these subspecies is important for indica-japonica hybridization breeding. In this study, subspecies and economic trait differentiation patterns were analyzed using morphological and molecular (InDel and Intron Length Polymorphism) data in F2 and F3 populations derived from indica-japonica hybridization. Populations were grown in Liaoning and Guangdong provinces, China, with F3 populations generated from F2 populations using bulk harvesting (BM) and single-seed descent methods (SSD). Segregation distortion was detected in F3-BM populations, but not in F3- SSD or in F2 populations. Superior performance was observed with respect to economic traits in Liaoning compared with that in Guangdong and 1 000-grain weight (KW), seed setting rate (SSR) and grain yield per plant (GYP) were significantly correlated with indica and japonica subspecies types. Analysis of molecular and morphological data demonstrated that the environment is the main factor giving rise to population differentiation in indica-japonica hybridization. In addition, we also found that KW, SSR and GYP are related to subspecies characteristics and kinship, which is possibly a significant factor resulting in economic trait differentiation and determining environmental adaptability. Our study has provided new insights into the process of population differentiation in these subspecies to inform indica-japonica hybridization breeding.