Discusses the significance of induction motor constant resistance (IM R) load on the lower part of the PV curve of a power system and determines the conditions for stable operation of IM R load using fuzzy techniques ...Discusses the significance of induction motor constant resistance (IM R) load on the lower part of the PV curve of a power system and determines the conditions for stable operation of IM R load using fuzzy techniques and load flow.展开更多
In this paper, responses of a new dual-induction resistivity logging-while-drilling (LWD) tool in 3D inhomogeneous formation models are simulated by the vectorfinite element method (VFEM), the influences of the bo...In this paper, responses of a new dual-induction resistivity logging-while-drilling (LWD) tool in 3D inhomogeneous formation models are simulated by the vectorfinite element method (VFEM), the influences of the borehole, invaded zone, surroundingstrata, and tool eccentricity are analyzed, and calibration loop parameters and calibrationcoefficients of the LWD tool are discussed. The results show that the tool has a greater depthof investigation than that of the existing electromagnetic propagation LWD tools and is moresensitive to azimuthal conductivity. Both deep and medium induction responses have linearrelationships with the formation conductivity, considering optimal calibration loop parametersand calibration coefficients. Due to the different depths of investigation and resolution, deepinduction and medium induction are affected differently by the formation model parameters,thereby having different correction factors. The simulation results can provide theoreticalreferences for the research and interpretation of the dual-induction resistivity LWD tools.展开更多
BNi-2/WC composite wear-resisting coating was prepared on carbon steel by the method of induction brazing.The microstructure and phase composition of the composite coating were analyzed,and the bonding strength and we...BNi-2/WC composite wear-resisting coating was prepared on carbon steel by the method of induction brazing.The microstructure and phase composition of the composite coating were analyzed,and the bonding strength and wear-resisting performance of the coating were tested.During the process of induction brazing,the tungsten carbide partially dissolves and reacts with the filler metal alloy to form NiW compound phase,which realizes the metallurgical combination of tungsten carbide and filler metal alloy.The matrix of the filler metal alloy consists of Ni solid solution and Ni_(3)B/Ni_(3)Si eutectic phase,and the metallurgical diffusion reaction occurs between the filler metal alloy and the steel matrix.The mechanical analysis results show that the self-strength of the composite coating reaches 140 MPa and the bonding strength of the filler metal alloy to the steel matrix reaches 360 MPa.The dry sand rubber wheel wear testing machine showed that the coating weight loss was only 0.2824 g,which was only 1/5 of the weight loss of 65 Mn matrix under the same conditions.展开更多
ZrN fihns were deposited on Si(111) and M2 steel by inductively coupled plasma (ICP)-enhanced RF magnetron sputtering. The effect of ICP power on the microstructure, mechanical properties and corrosion resistance ...ZrN fihns were deposited on Si(111) and M2 steel by inductively coupled plasma (ICP)-enhanced RF magnetron sputtering. The effect of ICP power on the microstructure, mechanical properties and corrosion resistance of ZrN films was investigated. When the ICP power is below 300 W, the ZrN films show a columnar structure. With the increase of ICP power, the texture coefficient (To) of the (111) plane, the nanohardness and elastic modulus of the films increase and reach the maximum at a power of 300 W. As the ICP Power exceeds 300 W, the films exhibit a ZrN and ZrNx mixed crystal structure without columnar grain while the nanohardness and elastic modulus of the films decrease. All the ZrN coated samples show a higher corrosion resistance than that of the bare M2 steel substrate in 3.5% NaCl electrolyte. The nanohardness and elastic modulus mostly depend on the crystalline structure and Tc of ZrN(111).展开更多
Antibiotic wastewater contains a variety of pollutant stressors that can induce and promote antibiotic resistance(AR)when released into the environment.Although these substances are mostly in concentrations lower than...Antibiotic wastewater contains a variety of pollutant stressors that can induce and promote antibiotic resistance(AR)when released into the environment.Although these substances are mostly in concentrations lower than those known to induce AR individually,it is possible that antibiotic wastewater discharge might still promote the AR transmission risk via additive or synergistic effects.However,the comprehensive effect of antibiotic wastewater on AR development has rarely been evaluated,and its treatment efficiency remains unknown.Here,samples were collected from different stages of a cephalosporin production wastewater treatment plant,and the potential AR induction effect of their chemical mixtures was explored through the exposure of the antibiotic-sensitive Escherichia coli K12 strain.Incubation with raw cephalosporin production wastewater significantly promoted mutation rates(3.6×10^(3)-9.3×10^(3)-fold)and minimum inhibition concentrations(6.0-6.7-fold)of E.coli against ampicillin and chloramphenicol.This may be attributed to the inhibition effect and oxidative stress of cephalosporin wastewater on E.coli.The AR induction effect of cephalosporin wastewater decreased after the coagulation sedimentation treatment and was completely removed after the full treatment process.A Pearson correlation analysis revealed that the reduction in the AR induction effect had a strong positive correlation with the removal of organics and biological toxicity.This indicates that the antibiotic wastewater treatment had a collaborative processing effect of conventional pollutants,toxicity,and the AR induction effect.This study illustrates the potential AR transmission risk of antibiotic wastewater and highlights the need for its adequate treatment.展开更多
The resistance and inductance of a wire array during an implosion are very important parameters of interest to:researchers. A variety of inductances and resistances directly affect the kinetic energy and resistance h...The resistance and inductance of a wire array during an implosion are very important parameters of interest to:researchers. A variety of inductances and resistances directly affect the kinetic energy and resistance heat energy coupled from a pulsed-power generator. In this paper, the inductance and resistance of a planar wire array during the Z-pinch process are analyzed. The inductance is calculated from the data obtained by a time-resolved soft X-ray framed camera, while the resistance is calculated through the voltage and the current of the wire array load combined with the variety of the inductance. The results show that the resistance of the load increases with the development of the implosion, and reaches its maximum at 0.29 ± 0.16Ω near the pinched time.展开更多
[Objective] The aim of this study was to isolate chitinase gene from Trichoderma atroviride strain SS003. [Method] With the aeciospore wall of armandii pine blister rust as inducer, chitinase gene was induced to expre...[Objective] The aim of this study was to isolate chitinase gene from Trichoderma atroviride strain SS003. [Method] With the aeciospore wall of armandii pine blister rust as inducer, chitinase gene was induced to express in Trichoderma atroviride cells. The cDNA fragment of chitinase gene was cloned by RT-PCR approach. [Result] The activity of chitinase induced reached 40.17 μg/10 min; and the specific fragment amplified was 834 bp in length and proved to be the fragment of chitinase gene by sequencing and sequence analysis. [Conclusion] The result showed the feasibility of isolating the full length of chitinase gene and its transformation, and further producing chitinase.展开更多
The transgenic rice, Zhongda 2, which was genetically modified from an indica rice line Zhuxian B by rice chitinase gene (RC24), had high resistance to rice sheath blight (Rhizoctonia solan!) in laboratory and a two-y...The transgenic rice, Zhongda 2, which was genetically modified from an indica rice line Zhuxian B by rice chitinase gene (RC24), had high resistance to rice sheath blight (Rhizoctonia solan!) in laboratory and a two-year field experiment. The pathogen could invade sheath of Zhongda 2 and induce symptoms of the disease. No difference was noted in time of penetration or incubation period between Zhongda 2 and non-transgenic rice control, Zhuxian B, but the hyphae lysate could be observed earlier than control. Its resistance expressed as to inhibit the growth of mycelium in host tissue. Fis from Zhongda 2(4) crossed with other five non-transgenic rice lines showed higher resistance than donor non-transgenic parents, but the resistance was different along with the different maternal parents.展开更多
Resistance to rice blast of transgenic rice lines harboring rice blast resistance gene Pi-d2 transformed from three different expression vectors of pCB6.3kb, pCB5.3kb and pZH01-2.72kb were analyzed. Nine advanced-gene...Resistance to rice blast of transgenic rice lines harboring rice blast resistance gene Pi-d2 transformed from three different expression vectors of pCB6.3kb, pCB5.3kb and pZH01-2.72kb were analyzed. Nine advanced-generation transgenic rice lines with Pi-d2 gene displayed various resistance to 39 rice blast strains, and the highest disease-resistant frequency reached 91.7%. Four early-generation homozygous transgenic lines with Pi-d2 gene exhibited resistance to more than 81.5% of 58 rice blast strains, showing the characteristic of wide-spectrum resistance. The transgenic embryonic calli selected by the crude toxin of rice blast fungus showed that the callus induction rate of immature embryo from transgenic rice plants decreased as the concentration of crude toxin in the culture medium increased. When the concentration of crude toxin reached 40%, the callus induction rate of immature embryo from transgenic lines was 49.3%, and that of the receptor control was 5%. The disease incidence of neck blast of the transgenic rice lines in fields under induction was 0% to 50%, indicating that the rice blast resistance of transgenic rice lines is much higher than that of the receptor control.展开更多
Growing awareness of the environmental damage caused by the use of chemical substances for plant disease control in agriculture has raised the need to study biological alternatives, such as activating the defense resp...Growing awareness of the environmental damage caused by the use of chemical substances for plant disease control in agriculture has raised the need to study biological alternatives, such as activating the defense response of plant crops by inducers not toxic to the environment. Trichoderma spp. are effective biocontrol agents for a number of soilborne pathogens, and are also known for their ability to enhance plant growth and to induce systemic resistance (ISR) in plants. In our laboratory we are studying the mechanism of induction of systemic responses against angular leaf spot disease of cucumber-Pseudomonas syringae pv. lachrymans (Psl), following application of T. asperellum to the root system. The protection afforded by the biocontrol agent is associated with concomitant accumulation of secondary metabolites of a phenolic nature and mRNA of two defense genes: the phenylpropanoid pathway gene phenylalanine ammonia lyase (PAL) and the octadecanoid pathway gene hydroxyperoxide lyase (HPL). Combining gene expression analysis, measurements of signal molecules levels and use of specific hormone inhibitors we could demonstrate that the JA/ethylene signaling pathway is involved in the systemic response induced by Trichoderma. A molecular analysis of Trichoderma genes and proteins differentially induced by root interaction reveals that molecules related to phytopathogenicity, but also known as elicitors of plant defense responses, are transiently activated in planta, enabling at the same time intracellular colonization and induction of systemic defense responses pathways. It appears that Trichoderma as a beneficial soil fungus has several efficient modes of action.展开更多
The effects of three different typical resistivity models(Spitzer, Z&L and M&G) on the performance of pulsed inductive acceleration plasma are studied. Numerical results show that their influences decrease with th...The effects of three different typical resistivity models(Spitzer, Z&L and M&G) on the performance of pulsed inductive acceleration plasma are studied. Numerical results show that their influences decrease with the increase of the plasma temperature. The significant discriminations among them appear at the plasma temperature lower than 2.5 eV, and the maximum gap of the pulsed inductive plasma accelerated efficiency is approximately 2.5%.Moreover, the pulsed inductive plasma accelerated efficiency is absolutely related to the dynamic impedance parameters, such as voltage, inductance, capacitance and flow rate. However, the distribution of the efficiency as a function of plasma temperature with three resistivity models has nothing to do with the dynamic impedance parameter.展开更多
Resistance of forty-one homozygous rice lines transformed with chitinase gene (RC24) and β-1,3 -glucanse gene β-1,3-Glu) to sheath blight was analyzed by inoculation. Among different lines, the resistance had signif...Resistance of forty-one homozygous rice lines transformed with chitinase gene (RC24) and β-1,3 -glucanse gene β-1,3-Glu) to sheath blight was analyzed by inoculation. Among different lines, the resistance had significant differences according to the result by cluster analysis. The lines could be categorized into resistant, moderately resistant, moderately susceptible and susceptible types, while 92.1% of which belonging to moderately resistant or moderately susceptible type. For different resistant or susceptible lines, the resistance to rice sheath blight was remarkable correlated with the chitinase activity of transgenic lines except resistant type lines, in which enzyme activity coded by target gene was lower than moderately resistant type. The chitinase activity of transgenic lines tested at different time after inoculation or different organs of the same plant was uniform, which suggested that the expression of chitinase gene was constitutive in nature. Check varieties chitinase activity would change at different time after inoculation and reach a peak at sometime, but it had no difference at various pans of the same plant.展开更多
The review paper summarizes the main results of the electromagnetic induction studies carried out in the Pannonian Basin and Carpathians during the last 30 years . The following conducting formations are discussed in ...The review paper summarizes the main results of the electromagnetic induction studies carried out in the Pannonian Basin and Carpathians during the last 30 years . The following conducting formations are discussed in detail : (1)Crustal conductors in the crystalline basement of the sedimentary basin mainly due to graphitic schist blocks clearly connected to the fracture tectonics of the area ; (2 ) Conducting layer in the lower crust probably due to free fluid deliberated by the dehydration process at temperature of 300-400℃during metamorphism ; (3 ) Conducting asthenosphere due to partial melting at the bottom of the lithosphere ; and (4) Conductivity increases due to olivine-spinel phase transition .All of these phenomena are strongly related to the special thermal state and tectonics of the Pannonian Basin and Carpathians.展开更多
Apoptosis of tumor cells have become a new standard for chemotherapy. It is useful to demonstrate induction of apoptosis in tumor cells by anti-cancer drugs in vivo. We reported the results of apoptosis induction in m...Apoptosis of tumor cells have become a new standard for chemotherapy. It is useful to demonstrate induction of apoptosis in tumor cells by anti-cancer drugs in vivo. We reported the results of apoptosis induction in murine tumor cell line S-180 and it's resistant cell line S-180R by adriamycin in different dose and different time. We found that apoptosis in S-180 cells could be induced by low dose of adriamycin, the apoptosis was started at 24 h. after the administration, and reached to 62.5% of the cells to apptosis until 72 h. Comparison with the parental cell line, only 13% of S-180R cells were apoptosed. At high dose, 20% of S-180R cells were apoptosed, whereas, almost all S-180 cells were killed in the same time. The lymphocytes were appeared in abdominal cavity of the mice after treatment of adriamycin for 24 h. It was very interested to find out that there was no lymphocyte left in the abdominal cavity of the mice with S-180R cells treated at high dose of adriamycin.展开更多
Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease c...Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.展开更多
Oxygen-free copper and pre-metalized graphite were brazed using CuNiSnP braze alloy by high frequency induction heating method. Interracial microstructures and reaction phases were analyzed by scanning electron micros...Oxygen-free copper and pre-metalized graphite were brazed using CuNiSnP braze alloy by high frequency induction heating method. Interracial microstructures and reaction phases were analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The strength and resistance of the joints were tested. It is found that when the brazing parameters are optimized, the structures of the joints are graphite/(Cu,Ni)/Ni(s.s)+NixPy/Cu3P+Cu(s.s) (including Sn)+eutectic structures (Cu3P+Ni3P+Cu(s.s)/Cu (s.s)/Cu). When the temperature increases to 750℃ or the holding time prolongs to 300 s, the eutectie structures disappear and the amount of Cu3P increases. The maximum shear strength of the joints is 5.2 MPa, which fracture at the interface of graphite and metallization. The resistance of the joints is no more than 5 mΩ.展开更多
By treatment with oxalate muskmelon cultivar “Wangwenxiang”, which is sensitive to squash mosaic virus (SqMV), develops systemic resistance to SqMV significantly. The challenge inoculation experiments indicate that ...By treatment with oxalate muskmelon cultivar “Wangwenxiang”, which is sensitive to squash mosaic virus (SqMV), develops systemic resistance to SqMV significantly. The challenge inoculation experiments indicate that the symptom of oxalate treated plants gets much slighter, the virus content is only 4% reduced to. The peroxidase activity increases by five times, three new isoperoxidases are induced, and lignin content increases by 82.9%. These results indicate that oxalate induces systemic resistance of muskmelon to SqMV while it induces increase of peroxidase activity, new isoperoxidases and content of lignin in the plants treated with oxalate.展开更多
Application of exogenous double-stranded RNA( dsRNA) for virus resistance in plants represents a very attractive alternative to virus resistant transgenic crops or pesticides targeting virus vectors.However,the instab...Application of exogenous double-stranded RNA( dsRNA) for virus resistance in plants represents a very attractive alternative to virus resistant transgenic crops or pesticides targeting virus vectors.However,the instability of dsRNA is a major challenge as spraying naked dsRNA onto plants provides protection against homologous viruses for only five days.Innovative approaches,such as the use of nanoparticles as carriers of dsRNA for improved stability and sustained release,are emerging as key disruptive technologies.Knowledge about the mechanism of entry,transport and processing of exogenously applied dsRNA in plants is still limited.In addition,cost of dsRNA and regulatory framework are still key influencers towards practical adoption of this technology.展开更多
Considering the high melting point and high stability of SrO,it has potential applications in the field of the preparation of titanium alloys.To improve the hydration resistance,SrO samples with different ZrO_(2)addit...Considering the high melting point and high stability of SrO,it has potential applications in the field of the preparation of titanium alloys.To improve the hydration resistance,SrO samples with different ZrO_(2)additions were fabricated after firing at 1750℃for 6 h.The phase,the microstructure,and the hydration resistance of the samples were researched.The results show that the sample with 33 mol%ZrO_(2)addition possesses good hydration resistance with the mass gain after hydration for 13 days of less than 0.42 mass%.The SrO crucible with 33 mol%ZrO_(2)addition does not react with TiNi alloy melt during melting,which provides a support for searching new refractories with good hydration resistance for induction melting titanium alloys.展开更多
文摘Discusses the significance of induction motor constant resistance (IM R) load on the lower part of the PV curve of a power system and determines the conditions for stable operation of IM R load using fuzzy techniques and load flow.
基金supported by the National Oil and Gas Major Projects(No.2011ZX05020-002)
文摘In this paper, responses of a new dual-induction resistivity logging-while-drilling (LWD) tool in 3D inhomogeneous formation models are simulated by the vectorfinite element method (VFEM), the influences of the borehole, invaded zone, surroundingstrata, and tool eccentricity are analyzed, and calibration loop parameters and calibrationcoefficients of the LWD tool are discussed. The results show that the tool has a greater depthof investigation than that of the existing electromagnetic propagation LWD tools and is moresensitive to azimuthal conductivity. Both deep and medium induction responses have linearrelationships with the formation conductivity, considering optimal calibration loop parametersand calibration coefficients. Due to the different depths of investigation and resolution, deepinduction and medium induction are affected differently by the formation model parameters,thereby having different correction factors. The simulation results can provide theoreticalreferences for the research and interpretation of the dual-induction resistivity LWD tools.
基金supported by the National Natural Science Foundation of China(Grant No.U2004186).
文摘BNi-2/WC composite wear-resisting coating was prepared on carbon steel by the method of induction brazing.The microstructure and phase composition of the composite coating were analyzed,and the bonding strength and wear-resisting performance of the coating were tested.During the process of induction brazing,the tungsten carbide partially dissolves and reacts with the filler metal alloy to form NiW compound phase,which realizes the metallurgical combination of tungsten carbide and filler metal alloy.The matrix of the filler metal alloy consists of Ni solid solution and Ni_(3)B/Ni_(3)Si eutectic phase,and the metallurgical diffusion reaction occurs between the filler metal alloy and the steel matrix.The mechanical analysis results show that the self-strength of the composite coating reaches 140 MPa and the bonding strength of the filler metal alloy to the steel matrix reaches 360 MPa.The dry sand rubber wheel wear testing machine showed that the coating weight loss was only 0.2824 g,which was only 1/5 of the weight loss of 65 Mn matrix under the same conditions.
文摘ZrN fihns were deposited on Si(111) and M2 steel by inductively coupled plasma (ICP)-enhanced RF magnetron sputtering. The effect of ICP power on the microstructure, mechanical properties and corrosion resistance of ZrN films was investigated. When the ICP power is below 300 W, the ZrN films show a columnar structure. With the increase of ICP power, the texture coefficient (To) of the (111) plane, the nanohardness and elastic modulus of the films increase and reach the maximum at a power of 300 W. As the ICP Power exceeds 300 W, the films exhibit a ZrN and ZrNx mixed crystal structure without columnar grain while the nanohardness and elastic modulus of the films decrease. All the ZrN coated samples show a higher corrosion resistance than that of the bare M2 steel substrate in 3.5% NaCl electrolyte. The nanohardness and elastic modulus mostly depend on the crystalline structure and Tc of ZrN(111).
基金supported by the National Key Research and Development Program of China(No.2017ZX07103-007)。
文摘Antibiotic wastewater contains a variety of pollutant stressors that can induce and promote antibiotic resistance(AR)when released into the environment.Although these substances are mostly in concentrations lower than those known to induce AR individually,it is possible that antibiotic wastewater discharge might still promote the AR transmission risk via additive or synergistic effects.However,the comprehensive effect of antibiotic wastewater on AR development has rarely been evaluated,and its treatment efficiency remains unknown.Here,samples were collected from different stages of a cephalosporin production wastewater treatment plant,and the potential AR induction effect of their chemical mixtures was explored through the exposure of the antibiotic-sensitive Escherichia coli K12 strain.Incubation with raw cephalosporin production wastewater significantly promoted mutation rates(3.6×10^(3)-9.3×10^(3)-fold)and minimum inhibition concentrations(6.0-6.7-fold)of E.coli against ampicillin and chloramphenicol.This may be attributed to the inhibition effect and oxidative stress of cephalosporin wastewater on E.coli.The AR induction effect of cephalosporin wastewater decreased after the coagulation sedimentation treatment and was completely removed after the full treatment process.A Pearson correlation analysis revealed that the reduction in the AR induction effect had a strong positive correlation with the removal of organics and biological toxicity.This indicates that the antibiotic wastewater treatment had a collaborative processing effect of conventional pollutants,toxicity,and the AR induction effect.This study illustrates the potential AR transmission risk of antibiotic wastewater and highlights the need for its adequate treatment.
基金supported by National Natural Science Fundation of China(No.10905047)
文摘The resistance and inductance of a wire array during an implosion are very important parameters of interest to:researchers. A variety of inductances and resistances directly affect the kinetic energy and resistance heat energy coupled from a pulsed-power generator. In this paper, the inductance and resistance of a planar wire array during the Z-pinch process are analyzed. The inductance is calculated from the data obtained by a time-resolved soft X-ray framed camera, while the resistance is calculated through the voltage and the current of the wire array load combined with the variety of the inductance. The results show that the resistance of the load increases with the development of the implosion, and reaches its maximum at 0.29 ± 0.16Ω near the pinched time.
基金Supported by Science Foundation from Southwest Forestry College(200524M)Natural Science Foundation of Yunan Province(2002C0047M)Key Scientific and Technological Project of Yunan Province(2003NG12)~~
文摘[Objective] The aim of this study was to isolate chitinase gene from Trichoderma atroviride strain SS003. [Method] With the aeciospore wall of armandii pine blister rust as inducer, chitinase gene was induced to express in Trichoderma atroviride cells. The cDNA fragment of chitinase gene was cloned by RT-PCR approach. [Result] The activity of chitinase induced reached 40.17 μg/10 min; and the specific fragment amplified was 834 bp in length and proved to be the fragment of chitinase gene by sequencing and sequence analysis. [Conclusion] The result showed the feasibility of isolating the full length of chitinase gene and its transformation, and further producing chitinase.
文摘The transgenic rice, Zhongda 2, which was genetically modified from an indica rice line Zhuxian B by rice chitinase gene (RC24), had high resistance to rice sheath blight (Rhizoctonia solan!) in laboratory and a two-year field experiment. The pathogen could invade sheath of Zhongda 2 and induce symptoms of the disease. No difference was noted in time of penetration or incubation period between Zhongda 2 and non-transgenic rice control, Zhuxian B, but the hyphae lysate could be observed earlier than control. Its resistance expressed as to inhibit the growth of mycelium in host tissue. Fis from Zhongda 2(4) crossed with other five non-transgenic rice lines showed higher resistance than donor non-transgenic parents, but the resistance was different along with the different maternal parents.
基金supported by the Program for Supporting New-century Excellent Talents of Ministry of Education, China (Grant No. NCET-04-0907)the Program for Supporting Development of Innovative Research Teams of Ministry of Education, China (Grant No. IRT0453)the Applied Basic Research Program of Sichuan Province, China (Grant No. 2008JY0023-1)
文摘Resistance to rice blast of transgenic rice lines harboring rice blast resistance gene Pi-d2 transformed from three different expression vectors of pCB6.3kb, pCB5.3kb and pZH01-2.72kb were analyzed. Nine advanced-generation transgenic rice lines with Pi-d2 gene displayed various resistance to 39 rice blast strains, and the highest disease-resistant frequency reached 91.7%. Four early-generation homozygous transgenic lines with Pi-d2 gene exhibited resistance to more than 81.5% of 58 rice blast strains, showing the characteristic of wide-spectrum resistance. The transgenic embryonic calli selected by the crude toxin of rice blast fungus showed that the callus induction rate of immature embryo from transgenic rice plants decreased as the concentration of crude toxin in the culture medium increased. When the concentration of crude toxin reached 40%, the callus induction rate of immature embryo from transgenic lines was 49.3%, and that of the receptor control was 5%. The disease incidence of neck blast of the transgenic rice lines in fields under induction was 0% to 50%, indicating that the rice blast resistance of transgenic rice lines is much higher than that of the receptor control.
文摘Growing awareness of the environmental damage caused by the use of chemical substances for plant disease control in agriculture has raised the need to study biological alternatives, such as activating the defense response of plant crops by inducers not toxic to the environment. Trichoderma spp. are effective biocontrol agents for a number of soilborne pathogens, and are also known for their ability to enhance plant growth and to induce systemic resistance (ISR) in plants. In our laboratory we are studying the mechanism of induction of systemic responses against angular leaf spot disease of cucumber-Pseudomonas syringae pv. lachrymans (Psl), following application of T. asperellum to the root system. The protection afforded by the biocontrol agent is associated with concomitant accumulation of secondary metabolites of a phenolic nature and mRNA of two defense genes: the phenylpropanoid pathway gene phenylalanine ammonia lyase (PAL) and the octadecanoid pathway gene hydroxyperoxide lyase (HPL). Combining gene expression analysis, measurements of signal molecules levels and use of specific hormone inhibitors we could demonstrate that the JA/ethylene signaling pathway is involved in the systemic response induced by Trichoderma. A molecular analysis of Trichoderma genes and proteins differentially induced by root interaction reveals that molecules related to phytopathogenicity, but also known as elicitors of plant defense responses, are transiently activated in planta, enabling at the same time intracellular colonization and induction of systemic defense responses pathways. It appears that Trichoderma as a beneficial soil fungus has several efficient modes of action.
基金Supported by the Fund of Science and Technology on Vacuum Technology and Physics Laboratory of Lanzhou Institute of Physics under Grant No YSC0715the National Natural Science Foundation of China under Grant No 62601210the Civil Aerospace Technology Research Project under Grant No D010509
文摘The effects of three different typical resistivity models(Spitzer, Z&L and M&G) on the performance of pulsed inductive acceleration plasma are studied. Numerical results show that their influences decrease with the increase of the plasma temperature. The significant discriminations among them appear at the plasma temperature lower than 2.5 eV, and the maximum gap of the pulsed inductive plasma accelerated efficiency is approximately 2.5%.Moreover, the pulsed inductive plasma accelerated efficiency is absolutely related to the dynamic impedance parameters, such as voltage, inductance, capacitance and flow rate. However, the distribution of the efficiency as a function of plasma temperature with three resistivity models has nothing to do with the dynamic impedance parameter.
文摘Resistance of forty-one homozygous rice lines transformed with chitinase gene (RC24) and β-1,3 -glucanse gene β-1,3-Glu) to sheath blight was analyzed by inoculation. Among different lines, the resistance had significant differences according to the result by cluster analysis. The lines could be categorized into resistant, moderately resistant, moderately susceptible and susceptible types, while 92.1% of which belonging to moderately resistant or moderately susceptible type. For different resistant or susceptible lines, the resistance to rice sheath blight was remarkable correlated with the chitinase activity of transgenic lines except resistant type lines, in which enzyme activity coded by target gene was lower than moderately resistant type. The chitinase activity of transgenic lines tested at different time after inoculation or different organs of the same plant was uniform, which suggested that the expression of chitinase gene was constitutive in nature. Check varieties chitinase activity would change at different time after inoculation and reach a peak at sometime, but it had no difference at various pans of the same plant.
文摘The review paper summarizes the main results of the electromagnetic induction studies carried out in the Pannonian Basin and Carpathians during the last 30 years . The following conducting formations are discussed in detail : (1)Crustal conductors in the crystalline basement of the sedimentary basin mainly due to graphitic schist blocks clearly connected to the fracture tectonics of the area ; (2 ) Conducting layer in the lower crust probably due to free fluid deliberated by the dehydration process at temperature of 300-400℃during metamorphism ; (3 ) Conducting asthenosphere due to partial melting at the bottom of the lithosphere ; and (4) Conductivity increases due to olivine-spinel phase transition .All of these phenomena are strongly related to the special thermal state and tectonics of the Pannonian Basin and Carpathians.
文摘Apoptosis of tumor cells have become a new standard for chemotherapy. It is useful to demonstrate induction of apoptosis in tumor cells by anti-cancer drugs in vivo. We reported the results of apoptosis induction in murine tumor cell line S-180 and it's resistant cell line S-180R by adriamycin in different dose and different time. We found that apoptosis in S-180 cells could be induced by low dose of adriamycin, the apoptosis was started at 24 h. after the administration, and reached to 62.5% of the cells to apptosis until 72 h. Comparison with the parental cell line, only 13% of S-180R cells were apoptosed. At high dose, 20% of S-180R cells were apoptosed, whereas, almost all S-180 cells were killed in the same time. The lymphocytes were appeared in abdominal cavity of the mice after treatment of adriamycin for 24 h. It was very interested to find out that there was no lymphocyte left in the abdominal cavity of the mice with S-180R cells treated at high dose of adriamycin.
基金Supported by the National Items of Research and Industrial Development of Transgenic Plants(J99-B-013)
文摘Soybean is one of the most important sources of edible oil and proteins in the world. However, it suffers from many kinds of fungal diseases which is a major limiting factor in soybean production. The fungal disease can be effectively controlled by breeding plant cultivars with genetic transformation. In this study, the resistance to Phytophthora sojae of five bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern Blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that four lines were more resistant to P sojae, whereas other one had no significant difference in comparison with the control. These transgenic soybean lines with enhanced resistance to P sojae will be useful in soybean resistance breeding.
基金Project(50705022) supported by the National Natural Science Foundation of ChinaProject supported by the Program for New Century Excellent Talents in University
文摘Oxygen-free copper and pre-metalized graphite were brazed using CuNiSnP braze alloy by high frequency induction heating method. Interracial microstructures and reaction phases were analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The strength and resistance of the joints were tested. It is found that when the brazing parameters are optimized, the structures of the joints are graphite/(Cu,Ni)/Ni(s.s)+NixPy/Cu3P+Cu(s.s) (including Sn)+eutectic structures (Cu3P+Ni3P+Cu(s.s)/Cu (s.s)/Cu). When the temperature increases to 750℃ or the holding time prolongs to 300 s, the eutectie structures disappear and the amount of Cu3P increases. The maximum shear strength of the joints is 5.2 MPa, which fracture at the interface of graphite and metallization. The resistance of the joints is no more than 5 mΩ.
文摘By treatment with oxalate muskmelon cultivar “Wangwenxiang”, which is sensitive to squash mosaic virus (SqMV), develops systemic resistance to SqMV significantly. The challenge inoculation experiments indicate that the symptom of oxalate treated plants gets much slighter, the virus content is only 4% reduced to. The peroxidase activity increases by five times, three new isoperoxidases are induced, and lignin content increases by 82.9%. These results indicate that oxalate induces systemic resistance of muskmelon to SqMV while it induces increase of peroxidase activity, new isoperoxidases and content of lignin in the plants treated with oxalate.
基金supported by Luzhou Branch of Sichuan Tobacco Cooperation
文摘Application of exogenous double-stranded RNA( dsRNA) for virus resistance in plants represents a very attractive alternative to virus resistant transgenic crops or pesticides targeting virus vectors.However,the instability of dsRNA is a major challenge as spraying naked dsRNA onto plants provides protection against homologous viruses for only five days.Innovative approaches,such as the use of nanoparticles as carriers of dsRNA for improved stability and sustained release,are emerging as key disruptive technologies.Knowledge about the mechanism of entry,transport and processing of exogenously applied dsRNA in plants is still limited.In addition,cost of dsRNA and regulatory framework are still key influencers towards practical adoption of this technology.
基金supported by the Independent Research and Development Project of the State Key Laboratory of Advanced Special Steel,Shanghai Key Laboratory of Advanced Ferrometallurgy,Shanghai University(SKLASS 2021-03)National Science Fund for Distinguished Young Scholars(No.52025041)National Natural Science Foundation of China(Grant No.52104305)
文摘Considering the high melting point and high stability of SrO,it has potential applications in the field of the preparation of titanium alloys.To improve the hydration resistance,SrO samples with different ZrO_(2)additions were fabricated after firing at 1750℃for 6 h.The phase,the microstructure,and the hydration resistance of the samples were researched.The results show that the sample with 33 mol%ZrO_(2)addition possesses good hydration resistance with the mass gain after hydration for 13 days of less than 0.42 mass%.The SrO crucible with 33 mol%ZrO_(2)addition does not react with TiNi alloy melt during melting,which provides a support for searching new refractories with good hydration resistance for induction melting titanium alloys.