Infected bone defects(IBDs)remains a challenging problem for orthopedists.Clinically,routine management for IBDs has two stages:debridement and systematic antibiotics administration to control infection,and secondary ...Infected bone defects(IBDs)remains a challenging problem for orthopedists.Clinically,routine management for IBDs has two stages:debridement and systematic antibiotics administration to control infection,and secondary grafting to repair bone defects.Whereas the efficacy is not satisfactory,because the overuse of antibiotics may lead to systemic toxicity,and the emergence of drug-resistant bacteria,as well as the secondary surgery would cause additional trauma and economic burden to the patients.Therefore,it is imperative to develop a novel scaffold for one-stage repair of IBDs.In this study,vancomycin(Van)was encapsulated into poly(lactic co-glycolic acid)(PLGA)microspheres through the double emulsion method,which were then loaded into the additively-manufactured porous tantalum(AM-Ta)through gelatin methacryloyl(GelMA)hydrogel to produce the composite Ta/GelMA hydrogel(Gel)/PLGA/vancomycin(Van)scaffolds for repairing IBDs.Physiochemical characterization of the newly-developed scaffold indicated that the releasing duration of Van was over 2 weeks.Biological experiments indicated good biocompatibility of the composite scaffold,as well as bacteriostasis and osteointegration properties,which showed great potential for clinical application.The construction of this novel scaffold would provide new sight into the development of orthopaedic implants,shedding a novel light on the treatment of IBDs.展开更多
基金supported by the Key research and development program of Hunan Province(Grant No.2020SK2008 and 2021GK2012)the Major science and technology projects of Changsha City(Grant No.kh2003016)+2 种基金Clinical research fund of National Clinical Research Center for Geriatric Disorders(Xiangya Hospital,Grant No.2020LNJJ15)The Natural Science Foundation Exploration Project of Zhejiang Province,China(Grant No.Y23H060040)the Natural Science Foundation of Hunan Province,China(Grant No.2022JJ30934).
文摘Infected bone defects(IBDs)remains a challenging problem for orthopedists.Clinically,routine management for IBDs has two stages:debridement and systematic antibiotics administration to control infection,and secondary grafting to repair bone defects.Whereas the efficacy is not satisfactory,because the overuse of antibiotics may lead to systemic toxicity,and the emergence of drug-resistant bacteria,as well as the secondary surgery would cause additional trauma and economic burden to the patients.Therefore,it is imperative to develop a novel scaffold for one-stage repair of IBDs.In this study,vancomycin(Van)was encapsulated into poly(lactic co-glycolic acid)(PLGA)microspheres through the double emulsion method,which were then loaded into the additively-manufactured porous tantalum(AM-Ta)through gelatin methacryloyl(GelMA)hydrogel to produce the composite Ta/GelMA hydrogel(Gel)/PLGA/vancomycin(Van)scaffolds for repairing IBDs.Physiochemical characterization of the newly-developed scaffold indicated that the releasing duration of Van was over 2 weeks.Biological experiments indicated good biocompatibility of the composite scaffold,as well as bacteriostasis and osteointegration properties,which showed great potential for clinical application.The construction of this novel scaffold would provide new sight into the development of orthopaedic implants,shedding a novel light on the treatment of IBDs.
