Among the human users of the Internet of Things,the hearing-impaired is a special group of people for whom normal information expression forms,such as voice and video are unaccessible,and most of them have some diffic...Among the human users of the Internet of Things,the hearing-impaired is a special group of people for whom normal information expression forms,such as voice and video are unaccessible,and most of them have some difficulty in understanding information in text form.The hearing-impaired are accustomed to receiving information expressed in sign language.For this situation,a new information expression form for the Internet of Things oriented toward the hearing-impaired is proposed in this paper,and the new expression is based on sign language video synthesis.Under the sign synthesis frame,three modules are necessary:constructing database,searching for appropriate sign language video units and transition units,and generating interpolated frames.With this method,text information could be transformed into sign language expression for the hearing-impaired.展开更多
We offer a new understanding of the scientific world and the contents of the basic question of philosophy,which is based on an analysis of the phenomenon of the specific information in the various components of the st...We offer a new understanding of the scientific world and the contents of the basic question of philosophy,which is based on an analysis of the phenomenon of the specific information in the various components of the structure of reality.It is shown that,in addition to material objects,processes,and events,in the real world are also intangible objects,processes and conditions that are in its information content.So the basic question of philosophy is proposed to treat the relationship as a tangible and intangible component of reality.More succinctly it can be formulated as the ratio of matter and information.This allows you to point to some new challenges studying the phenomenon of information in various components of the structure of reality.展开更多
OBJECTIVE To investigate icariside(ICS)Ⅱ protects against PC12 cel damage induced by oxygen-glucose deprivation and reoxygenation and explore its mechanism.METHODS The oxidative stress injury model was induced by oxy...OBJECTIVE To investigate icariside(ICS)Ⅱ protects against PC12 cel damage induced by oxygen-glucose deprivation and reoxygenation and explore its mechanism.METHODS The oxidative stress injury model was induced by oxygen-glucose deprivation/reoxygenation(OGD/R) 2 h/24 h in PC12 cells.N-acetyl-lcysteine(NAC),a classical anti-oxidant,was used as positive control.Pharmacodynamic experimental study groups as follows:control,control+ICS Ⅱ50 μmol·L^(-1),OGD/R,OGD/R+ICSⅡ 12.5 μmol·L^(-1),OGD/R + ICS Ⅱ 25 μmol·L^(-1),OGD/R + ICS Ⅱ50 μmol·L^(-1),and OGD/R+NAC 100 μmol·L^(-1) groups.Cell viability and lactate dehydrogenase(LDH) leakage rate were measured by MTT assay and LDH ELISA kit,respectively.Moreover,reactive oxygen species(ROS) ELISA kit was used for detection of intracellular ROS generation,Mito-SOX fluorescence staining was used for detecting production of ROS in mitochondria and mitochondrial membrane potential(MMP)was detected by rhodamine 123 dye.In addition,PC12 cells apoptosis was detected by one-step TUNEL assay.Furthermore,the expressions of nuclear factor erythroid 2-related factors(Nrf2),Keap1,HO^(-1),NQO^(-1),silent information regulator 3(SIRT3),IDH2,Bax,Bcl-2 and caspase 3 were detected by Western blotting analysis.RESULTS The results of MTT and LDH assay showed that OGD/R reduced the cell viability and improved LDH release compared with the control or ICSⅡ 50 μmol·L^(-1) alone(P<0.01).Meanwhile,OGD/R not only increased intracellular and mitochondrial ROS generation,but also elevated the fluorescence intensity of TUNEL staining,at the same time,the MMP was declined when challenged by OGD/R.Furthermore,the Western blotting results showed that OGD/R induced the increase in the expression of cytoplasm-Nrf2,Keap1,Bax and cleaved-caspase 3 level,while the decrease in the expression of nucleus-Nrf2,HO^(-1),NQO^(-1),SIRT3,IDH2 and Bcl-2(P<0.05).However,ICS Ⅱ significantly increased the viability of PC12 cells and reduced LDH leakage(P<0.01).Notably,ICS Ⅱ also suppressed ROS generation both in the intracellular and mitochondria,as well as restored MMP.It was also worthy to note that ICS Ⅱ decreased the expressions of cytoplasmNrf2,Keap1,Bax and the level of cleaved-caspase3,whereas,it increased the expressions of nucleus-Nrf2,HO^(-1),NQO^(-1),SIRT3,IDH2 and Bcl-2(P<0.05).CONCLUSION ICSⅡ reduced OGD/Rinduced oxidative damage in PC12 cells under the laboratory conditions,and its underlying mechanism may be related to the regulation of Nrf2/SIRT3 signaling pathway.展开更多
User-analysis techniques are mainly used to recommend friends and information. This paper discusses the data characteristics of microblog users and describes a multidimensional user rec- ommendation algorithm that tak...User-analysis techniques are mainly used to recommend friends and information. This paper discusses the data characteristics of microblog users and describes a multidimensional user rec- ommendation algorithm that takes into account microblog length, relativity between microblog and users, and familiarity between users. The experimental results show that this multidi- mensional algorithm is more accurate than a traditional recom- mendation algorithm.展开更多
基金supported by the National Natural Science Foundation of China(Nos.60825203,60973056,60973057,U0935004)National Technology Support Project(2007BAH13B01)+2 种基金Beijing Municipal Natural Science Foundation(4102009)Scientific Research Common Program of Beijing Municipal Commission of Education(KM200710005023)PHR(IHLB)
文摘Among the human users of the Internet of Things,the hearing-impaired is a special group of people for whom normal information expression forms,such as voice and video are unaccessible,and most of them have some difficulty in understanding information in text form.The hearing-impaired are accustomed to receiving information expressed in sign language.For this situation,a new information expression form for the Internet of Things oriented toward the hearing-impaired is proposed in this paper,and the new expression is based on sign language video synthesis.Under the sign synthesis frame,three modules are necessary:constructing database,searching for appropriate sign language video units and transition units,and generating interpolated frames.With this method,text information could be transformed into sign language expression for the hearing-impaired.
文摘We offer a new understanding of the scientific world and the contents of the basic question of philosophy,which is based on an analysis of the phenomenon of the specific information in the various components of the structure of reality.It is shown that,in addition to material objects,processes,and events,in the real world are also intangible objects,processes and conditions that are in its information content.So the basic question of philosophy is proposed to treat the relationship as a tangible and intangible component of reality.More succinctly it can be formulated as the ratio of matter and information.This allows you to point to some new challenges studying the phenomenon of information in various components of the structure of reality.
基金National Natural Science Foundation of China(81560666)Program for Excellent Young Talents of Zunyi Medical Uiverstity(15zy-002)+1 种基金Science and Technology Innovation Talent Team of Guizhou Province(20154023)the ″Hundred″Level of High-level Innovative Talents in Guizhou Province(QKHRCPT 20165684)；and Program forChangjiang Scholars and Innovative ResearchTeam in University of China(IRT一17R113).
文摘OBJECTIVE To investigate icariside(ICS)Ⅱ protects against PC12 cel damage induced by oxygen-glucose deprivation and reoxygenation and explore its mechanism.METHODS The oxidative stress injury model was induced by oxygen-glucose deprivation/reoxygenation(OGD/R) 2 h/24 h in PC12 cells.N-acetyl-lcysteine(NAC),a classical anti-oxidant,was used as positive control.Pharmacodynamic experimental study groups as follows:control,control+ICS Ⅱ50 μmol·L^(-1),OGD/R,OGD/R+ICSⅡ 12.5 μmol·L^(-1),OGD/R + ICS Ⅱ 25 μmol·L^(-1),OGD/R + ICS Ⅱ50 μmol·L^(-1),and OGD/R+NAC 100 μmol·L^(-1) groups.Cell viability and lactate dehydrogenase(LDH) leakage rate were measured by MTT assay and LDH ELISA kit,respectively.Moreover,reactive oxygen species(ROS) ELISA kit was used for detection of intracellular ROS generation,Mito-SOX fluorescence staining was used for detecting production of ROS in mitochondria and mitochondrial membrane potential(MMP)was detected by rhodamine 123 dye.In addition,PC12 cells apoptosis was detected by one-step TUNEL assay.Furthermore,the expressions of nuclear factor erythroid 2-related factors(Nrf2),Keap1,HO^(-1),NQO^(-1),silent information regulator 3(SIRT3),IDH2,Bax,Bcl-2 and caspase 3 were detected by Western blotting analysis.RESULTS The results of MTT and LDH assay showed that OGD/R reduced the cell viability and improved LDH release compared with the control or ICSⅡ 50 μmol·L^(-1) alone(P<0.01).Meanwhile,OGD/R not only increased intracellular and mitochondrial ROS generation,but also elevated the fluorescence intensity of TUNEL staining,at the same time,the MMP was declined when challenged by OGD/R.Furthermore,the Western blotting results showed that OGD/R induced the increase in the expression of cytoplasm-Nrf2,Keap1,Bax and cleaved-caspase 3 level,while the decrease in the expression of nucleus-Nrf2,HO^(-1),NQO^(-1),SIRT3,IDH2 and Bcl-2(P<0.05).However,ICS Ⅱ significantly increased the viability of PC12 cells and reduced LDH leakage(P<0.01).Notably,ICS Ⅱ also suppressed ROS generation both in the intracellular and mitochondria,as well as restored MMP.It was also worthy to note that ICS Ⅱ decreased the expressions of cytoplasmNrf2,Keap1,Bax and the level of cleaved-caspase3,whereas,it increased the expressions of nucleus-Nrf2,HO^(-1),NQO^(-1),SIRT3,IDH2 and Bcl-2(P<0.05).CONCLUSION ICSⅡ reduced OGD/Rinduced oxidative damage in PC12 cells under the laboratory conditions,and its underlying mechanism may be related to the regulation of Nrf2/SIRT3 signaling pathway.
文摘User-analysis techniques are mainly used to recommend friends and information. This paper discusses the data characteristics of microblog users and describes a multidimensional user rec- ommendation algorithm that takes into account microblog length, relativity between microblog and users, and familiarity between users. The experimental results show that this multidi- mensional algorithm is more accurate than a traditional recom- mendation algorithm.
