Aim： To evaluate whether inhibin-B can predict the outcome of a microsurgical epidymal sperm aspiration （MESA） procedure in patients with suspected primary obstructive azoospermia （OA） and if inhibin-B can replac...Aim： To evaluate whether inhibin-B can predict the outcome of a microsurgical epidymal sperm aspiration （MESA） procedure in patients with suspected primary obstructive azoospermia （OA） and if inhibin-B can replace testicular biopsy in the diagnostic work-up of these patients. Methods： Inhibin-B levels and testicular biopsy scores were related to the outcome of MESA in 43 patients with suspected primary OA. MESA was considered to be successful when epididymal sperm could be identified during the procedure. Results： Spermatozoa were present in the epididymal aspirate in 28 out of the 43 patients （65%）. lnhibin-B values were not significantly different in patients with successful or unsuccessful MESA. The modified Johnsen score, however, was significantly lower in patients with unsuccessful MESA （P = 0.003）. A rete testis obstruction or epididymal malfunctioning was found in 15% of patients with suspected primary OA, reflected by unsuccessful MESA despite normal inhibin-B levels and normal testicular histology. Conclusion： Inhibin-B cannot replace testicular biopsy as a diagnostic tool in the work-up of patients with suspected primary OA. Testicular biopsy is useful in identifying patients with spermatogenic arrest, who might have normal inhibin-B values.展开更多
目的探讨脂肪间充质干细胞对卵巢早衰小鼠卵巢内分泌功能及颗粒细胞凋亡的影响。方法无特定病原体(specific pathogen free,SPF)级昆明种小鼠36只,将其采用随机数字表法分为3组,即假手术组、卵巢早衰组以及脂肪间充质干细胞组,每组各12...目的探讨脂肪间充质干细胞对卵巢早衰小鼠卵巢内分泌功能及颗粒细胞凋亡的影响。方法无特定病原体(specific pathogen free,SPF)级昆明种小鼠36只,将其采用随机数字表法分为3组,即假手术组、卵巢早衰组以及脂肪间充质干细胞组,每组各12只。卵巢早衰组以及脂肪间充质干细胞组小鼠腹腔注射环磷酰胺和白消安建立卵巢早衰模型,假手术组小鼠腹腔注射0.9%氯化钠溶液。脂肪间充质干细胞组小鼠于造模成功后第1天起每7天经尾静脉注射1ml浓度为1.0×10^(9)/L脂肪间充质干细胞悬液,共注射4次。比较造模前1天、造模成功后14天和造模成功后28天3组小鼠血清雌二醇、孕酮、促卵泡生成素、黄体生成素以及抑制素B水平变化,并比较治疗后3组小鼠对卵巢病理损伤的影响和卵巢中颗粒细胞的凋亡。结果造模成功后14天,卵巢早衰组以及脂肪间充质干细胞组小鼠的血清雌二醇、孕酮以及抑制素B水平均低于假手术组(P均<0.05),促卵泡生成素、黄体生成素水平均高于假手术组(P均<0.05),且造模成功后28天卵巢早衰组小鼠的血清雌二醇、孕酮以及抑制素B水平均低于脂肪间充质干细胞组(33.57±1.98ng/L vs 41.66±1.70ng/L,t=-5.945,P<0.05;7.57±1.12ng/L vs 10.84±1.12ng/L,t=-4.056,P<0.05;110.58±4.18ng/L vs 129.86±2.97ng/L,t=-7.394,P<0.05),促卵泡生成素、黄体生成素水平均高于脂肪间充质干细胞组(5.15±0.20IU/L vs 3.61±0.12IU/L,t=10.401,P<0.05;510.94±7.11IU/L vs 410.14±5.45IU/L,t=22.407,P<0.05)。经脂肪间充质干细胞移植治疗后,卵巢早衰小鼠卵巢病理损伤明显减轻,且凋亡的颗粒细胞数量明显减少。结论脂肪间充质干细胞通过改善卵巢早衰小鼠卵巢内分泌功能,减少颗粒细胞凋亡,对卵巢早衰具有治疗作用。展开更多
文摘Aim： To evaluate whether inhibin-B can predict the outcome of a microsurgical epidymal sperm aspiration （MESA） procedure in patients with suspected primary obstructive azoospermia （OA） and if inhibin-B can replace testicular biopsy in the diagnostic work-up of these patients. Methods： Inhibin-B levels and testicular biopsy scores were related to the outcome of MESA in 43 patients with suspected primary OA. MESA was considered to be successful when epididymal sperm could be identified during the procedure. Results： Spermatozoa were present in the epididymal aspirate in 28 out of the 43 patients （65%）. lnhibin-B values were not significantly different in patients with successful or unsuccessful MESA. The modified Johnsen score, however, was significantly lower in patients with unsuccessful MESA （P = 0.003）. A rete testis obstruction or epididymal malfunctioning was found in 15% of patients with suspected primary OA, reflected by unsuccessful MESA despite normal inhibin-B levels and normal testicular histology. Conclusion： Inhibin-B cannot replace testicular biopsy as a diagnostic tool in the work-up of patients with suspected primary OA. Testicular biopsy is useful in identifying patients with spermatogenic arrest, who might have normal inhibin-B values.
文摘目的探讨脂肪间充质干细胞对卵巢早衰小鼠卵巢内分泌功能及颗粒细胞凋亡的影响。方法无特定病原体(specific pathogen free,SPF)级昆明种小鼠36只,将其采用随机数字表法分为3组,即假手术组、卵巢早衰组以及脂肪间充质干细胞组,每组各12只。卵巢早衰组以及脂肪间充质干细胞组小鼠腹腔注射环磷酰胺和白消安建立卵巢早衰模型,假手术组小鼠腹腔注射0.9%氯化钠溶液。脂肪间充质干细胞组小鼠于造模成功后第1天起每7天经尾静脉注射1ml浓度为1.0×10^(9)/L脂肪间充质干细胞悬液,共注射4次。比较造模前1天、造模成功后14天和造模成功后28天3组小鼠血清雌二醇、孕酮、促卵泡生成素、黄体生成素以及抑制素B水平变化,并比较治疗后3组小鼠对卵巢病理损伤的影响和卵巢中颗粒细胞的凋亡。结果造模成功后14天,卵巢早衰组以及脂肪间充质干细胞组小鼠的血清雌二醇、孕酮以及抑制素B水平均低于假手术组(P均<0.05),促卵泡生成素、黄体生成素水平均高于假手术组(P均<0.05),且造模成功后28天卵巢早衰组小鼠的血清雌二醇、孕酮以及抑制素B水平均低于脂肪间充质干细胞组(33.57±1.98ng/L vs 41.66±1.70ng/L,t=-5.945,P<0.05;7.57±1.12ng/L vs 10.84±1.12ng/L,t=-4.056,P<0.05;110.58±4.18ng/L vs 129.86±2.97ng/L,t=-7.394,P<0.05),促卵泡生成素、黄体生成素水平均高于脂肪间充质干细胞组(5.15±0.20IU/L vs 3.61±0.12IU/L,t=10.401,P<0.05;510.94±7.11IU/L vs 410.14±5.45IU/L,t=22.407,P<0.05)。经脂肪间充质干细胞移植治疗后,卵巢早衰小鼠卵巢病理损伤明显减轻,且凋亡的颗粒细胞数量明显减少。结论脂肪间充质干细胞通过改善卵巢早衰小鼠卵巢内分泌功能,减少颗粒细胞凋亡,对卵巢早衰具有治疗作用。
