The melt treatment of A1-7%Si alloy by the flux injection method in a resistance furnance was investigated. The experimental results show that the flux injection method can realize the melt treatment of A1-7%Si alloy....The melt treatment of A1-7%Si alloy by the flux injection method in a resistance furnance was investigated. The experimental results show that the flux injection method can realize the melt treatment of A1-7%Si alloy. In purification, the flux injection method is superior to the standard lance degassing method. For the injection flux, the purifier Na2SiF6 is more powerful than Na3A1F6, the modifiers SrCl2, and R.E. Cl3 are quick acting, like NaF, but less powerful, the grain refiner containing K2TiF6 and KBF4 with Ti/B=1 is more potent than that with Ti/B=5. The analytical results indicate that the mass-transfer rate for the flux injection method is much higher than that for the conventional method .展开更多
The pellet injection experiments for fuelling and diagnostics have been carried out on the HL-1M tokamak. The eight-pellet injector was installed on HL-1M. A reliable monitordetector and camera system was set up to ta...The pellet injection experiments for fuelling and diagnostics have been carried out on the HL-1M tokamak. The eight-pellet injector was installed on HL-1M. A reliable monitordetector and camera system was set up to take initial pellet photographs and measure the initial pellet speed and size. High fuelling efficiency of 60 % - 100 % and a density profile with a peaking factor of 1.8 - 2.0 were obtained. The maximum density close to 10^14/cm^3 in HL-1M was achieved with newly optimized combined fuelling techniques. Two typical models of pellet ablation have been utilized for simulative calculation of the ablation rates in HL-1M. In comparison with the distribution of the measured Hα emission intensity from the digital data of the CCD camera, the experimental result seems more optimistic for core fuelling than theoretical predictions by the two models. The safety factor profile q(r) has been extracted from the information provided by the CCD camera during the pellet injection. The reliability of the measured results depends mainly on the calibration of the imaging space position. Based on the calibration, the measured q-profile becomes more reasonable than those published previously for the same shot number and same photograph.展开更多
1 Scope This standard specifies the classification, techni cal requirements, test methods, quality appraisal pro- cedures, packing, marking, transportation, storage, quality certificate, and construction manual of non...1 Scope This standard specifies the classification, techni cal requirements, test methods, quality appraisal pro- cedures, packing, marking, transportation, storage, quality certificate, and construction manual of nonwater injection mix for blast furnace.展开更多
An analysis is performed to study the heat transfer characteristics of steady two-dimensional boundary layer flow past a moving permeable flat plate in a nanofluid. The effects of uniform suction and injection on the ...An analysis is performed to study the heat transfer characteristics of steady two-dimensional boundary layer flow past a moving permeable flat plate in a nanofluid. The effects of uniform suction and injection on the flow field and heat transfer characteristics are numerically studied by using an implicit finite difference method. It is found that dual solutions exist when the plate and the free stream move in the opposite directions. The results indicate that suction delays the boundary layer separation, while injection accelerates it.展开更多
Tongluojiunao (TLJN) is an herbal medicine consisting of two main components, geniposide and ginsenoside Rg1. TLJN has been shown to protect primary cultured hippocampal neurons. How-ever, its mechanism of action re...Tongluojiunao (TLJN) is an herbal medicine consisting of two main components, geniposide and ginsenoside Rg1. TLJN has been shown to protect primary cultured hippocampal neurons. How-ever, its mechanism of action remains unclear. In the present study, primary cultured hippocampal neurons treated with Aβ1-42 (10 μmol/L) signiifcantly increased the release of lactate dehydroge-nase, which was markedly reduced by TLJN (2 μL/mL), speciifcally by the component geniposide (26 μmol/L), but not ginsenoside Rg1 (2.5 μmol/L). hTe estrogen receptor inhibitor, ICI182780 (1 μmol/L), did not block TLJN-or geniposide-mediated decrease of lactate dehydrogenase under Aβ1-42-exposed conditions. However, the phosphatidyl inositol 3-kinase or mitogen-activated protein kinase pathway inhibitor, LY294002 (50 μmol/L) or U0126 (10 μmol/L), respectively blo cked the decrease of lactate dehydrogenase mediated by TLJN or geniposide. hTerefore, these results suggest that the non-classical estrogen pathway (i.e., phosphatidyl inositol 3-kinase or mitogen-activated protein kinase) is involved in the neuroprotective effect of TLJN, speciifcally its component, geniposide, against Aβ1-42-mediated cell death in primary cultured hippocampal neurons.展开更多
Objective: To explore the effect of ligustrazine injection (LI) on serum levels of interleukin-4 (IL-4) and interferon- -γ (IFN- -γ ) in patients with bronchial asthma and determine the mechanism of action of...Objective: To explore the effect of ligustrazine injection (LI) on serum levels of interleukin-4 (IL-4) and interferon- -γ (IFN- -γ ) in patients with bronchial asthma and determine the mechanism of action of LI in preventing and treating asthma. Methods: Sixty-eight patients with mild or moderate bronchial asthma were assigned to two groups equally according to their sequence number, odd or even. The conventional treatment was administered to both groups, and LI was given to the treatment group by ultrasonic spray inhalation twice a day but not to the control group. The therapeutic course for all was 2 weeks. Further, 30 healthy subjects who had no history of smoking were enrolled as the normal control group. The clinical condition scores, frequency of attacks and dosage of Terbutaline inhaled were scored and recorded on the first day of hospitalization (before treatment) and after treatment. At the same time, the indexes of lung function, including forced expiratory volume in one second (FEV1), forced expiratory volume in one second to forced vital capacity ratio (FEV1%) and the peak expiratory flow (PEF) were determined before treatment. The levels of IL-4 and IFN--γ in peripheral blood were detected by ELISA before and after treatment, and compared with that of the healthy control group. Results: After treatment, the clinical condition scores were found to be lower, indexes of lung function were elevated, but serum level of IL-4 and ratio of IL-4/IFN-γwere reduced in both groups, showing significant differences as compared to those before treatment (P〈0.05). However, the changes in all the indexes were more significant in the treatment group than in the control group, also showing statistical significance (P〈0.05). No significant difference was revealed when IFN--γ levels were compared before and after treatment in both groups, though a lowering trend could be seen, significant difference could not be found in the comparison of IFN--γ levels between groups after treatment (P〉0.05). Conclusion: LI shows good clinical effect in treating bronchial asthma, and its mechanism might be related to the suppression of the synthesis of IL-4, thus leading to the inhibition of TH2 cell subset preponderant response and immune equilibrium regulation.展开更多
Fused filament fabrication(FFF)is one of the additive manufacturing processes which has gained more interest because of its simplicity and low-cost.This technology is similar to the conventional metal injection mouldi...Fused filament fabrication(FFF)is one of the additive manufacturing processes which has gained more interest because of its simplicity and low-cost.This technology is similar to the conventional metal injection moulding(MIM)process,consisting of the feedstock preparation of metal powder and polymer binders,followed by layer-by-layer 3D printing(FFF)or injection(MIM)to create green parts and,finally,debinding and sintering.Moreover,both technologies provide near-dense parts.This work presents an in-depth study of the processing method’s influence.The porosity,microstructure,hardness,corrosion,and tribocorrosion behaviour are compared for 17-4 PH SS samples processed from powder by additive manufacturing using FFF and MIM,as well as conventional powder metallurgy(PM)samples.MIM samples exhibited the highest macro and microhardness,while corrosion behaviour was similar for both MIM and FFF samples,but superior in comparison to conventional PM samples.However,the FFF-as fabricated samples displayed a significant improvement in tribocorrosion resistance that could be explained by the higher proportion of delta ferrite and retained austenite in their microstructure.展开更多
OBJECTIVE: To investigate the protective effect and possible mechanism of Xuebijing Injection on myocardial injury in patients with sepsis, and to evaluate its prognostic implications.METHODS: Patients with septic myo...OBJECTIVE: To investigate the protective effect and possible mechanism of Xuebijing Injection on myocardial injury in patients with sepsis, and to evaluate its prognostic implications.METHODS: Patients with septic myocardial injury were recruited, and were randomly divided into two groups: treatment group and control group. All patients in two groups received conventional cluster treatment, the patients in treatment group additional received Xuebijing injection dissolved in0.9% sodium chloride injection, and the patients in control group received the same amount of 0.9%sodium chloride injection. At the beginning of treatment and 3, 7 and 10-day after treatment, lab-oratory indicators of cardiac troponin Ⅰ(cTnI),N-terminal pro B-type natriuretic peptide(NT-pro BNP) and procalcitonin(PCT) were respectively tested in venous blood. The patient's length of stay in Intensive Care Unit(ICU) and the mortality in 28 days were recorded.RESULTS: At 3, 7 and 10-day after treatment, the improvements of c Tn I, NT-pro BNP and PCT in treatment group were better than those in control group, and the differences were statistically significant(P < 0.05). The mortality of treatment group in28 days was not significantly different from that of control group(P > 0.05). The ICU length of stay of treatment group was shorter than that of control group(P > 0.05).CONCLUSION: Xuebijing injection could improve the levels of c Tn I, NT-pro BNP and PCT in patients with septic myocardial injury.and it had a protective effect on myocardial injury.展开更多
In this paper, the desulphurisation of high sulphur low-rank coal is proposed as a raw material for pulverised coal injection technology. Therefore, the influence of oxidant linear velocity and the size of the coal gr...In this paper, the desulphurisation of high sulphur low-rank coal is proposed as a raw material for pulverised coal injection technology. Therefore, the influence of oxidant linear velocity and the size of the coal grain was investigated in a fluidised bed. The hydrodynamic parameters of the fluidised bed including: porosity, Sherwood criterion (diffusion Nusselt number), and mass transfer coefficient (external surface) were calculated. Furthermore, the study examined the effects of intensity and efficiency on the desulphurised coal properties; organic matter, ash, and volatile matter contents. The key changes during the conversion of pyritic sulphur and coal organic matter were subsequently examined. The results showed that the sulphur content (St^d 3.16 wt%) of the low-rank coal, was transformed to (St^d 〈 1.5 wt%) after desulfurization. Other enhanced properties were: V^daf ≤ 38.0 wt%; A^d 〈 10.0 wt%, now suitable for pulverised coal injection technology.展开更多
The diffusion coefficient of natural gas in foamy oil is one of the key parameters to evaluate the feasibility of gas injection for enhanced oil recovery in foamy oil reservoirs. In this paper, a PVT cell was used to ...The diffusion coefficient of natural gas in foamy oil is one of the key parameters to evaluate the feasibility of gas injection for enhanced oil recovery in foamy oil reservoirs. In this paper, a PVT cell was used to measure diffusion coefficients of natural gas in Venezuela foamy oil at high pressures, and a new method for deter- mining the diffusion coefficient in the foamy oil was de- veloped on the basis of experimental data. The effects of pressure and the types of the liquid phase on the diffusion coefficient of the natural gas were discussed. The results indicate that the diffusion coefficients of natural gas in foamy oil, saturated oil, and dead oil increase linearly with increasing pressure. The diffusion coefficient of natural gas in the foamy oil at 20 MPa was 2.93 times larger than that at 8.65 MPa. The diffusion coefficient of the natural gas in dead oil was 3.02 and 4.02 times than that of the natural gas in saturated oil and foamy oil when the pressure was 20 MPa. However, the gas content of foamy oil was 16.9 times higher than that of dead oil when the dissolution time and pressure were 20 MPa and 35.22 h, respectively.展开更多
Objective:To study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning(RDN)Injection.Methods:In this study,the active fraction of RDN Injection was screened by the LPS-induced mouse...Objective:To study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning(RDN)Injection.Methods:In this study,the active fraction of RDN Injection was screened by the LPS-induced mouse endotoxin shock model.The chemical constituents were isolated by chromatography on HP-20 macroporous adsorptive resins,silica gel,ODS columns and reverse phase MPLC and HPLC repeatedly,and their structures were elucidated based on spectroscopic analysis(HR-ESI-MS,NMR,ECD)and chemical methods.Meanwhile,we evaluated the anti-inflammatory activities of the isolates by measuring their inhibitory effects on TNF-αproduction in LPS stimulated RAW 264.7 macrophages.Results:The 95%ethanol eluate of RDN Injection by the macroporous adsorption resin column was proved to be the antipyretic and anti-inflammatory active fraction of this injection.A novel iridoid,named jasminoide A(1),and a new guaiane sesquiterpenoid,named(1 R,7 R,8 S,10 R)-7,8,11-trihydroxy-4-guaien-3-one(2),were isolated from Reduning injection,and compound 2 can inhibit TNF-αproduction with IC50 values of 72.24μmol/L.Conclusion:In this study,two new terpenoids were isolated from Reduning Injection,and compound 2 showed inhibitory activity against LPS-activated TNF-αproduction in RAW 264.7 cells in vitro.展开更多
To solve the problems caused by military software security issues,this paper firstly introduces a new software fault injection technique,namely main static fault injection method:program mutation.And then source code ...To solve the problems caused by military software security issues,this paper firstly introduces a new software fault injection technique,namely main static fault injection method:program mutation.And then source code for testing this algorithm is put forward.On this basis buffer overflow testing based on program mutation is conducted.Finally several military software source codes for buffer overflow testing are tested using deficiency tracking system(DTS)tool,Experimental results show the effectiveness of the proposed algorithm.展开更多
In this thesis a reviwe is presented for the advances of catalytic spec trophotometric method, three new catalytic reaction systems were presented, and its rela live catalytic spectrophotometric methods have been esta...In this thesis a reviwe is presented for the advances of catalytic spec trophotometric method, three new catalytic reaction systems were presented, and its rela live catalytic spectrophotometric methods have been established by flow injection tech nique. Particulary the relative chemometric methods and theories were used in the field of kinetic - catalytic analysis, in order to solve the problems of multicomponents analysis.展开更多
文摘The melt treatment of A1-7%Si alloy by the flux injection method in a resistance furnance was investigated. The experimental results show that the flux injection method can realize the melt treatment of A1-7%Si alloy. In purification, the flux injection method is superior to the standard lance degassing method. For the injection flux, the purifier Na2SiF6 is more powerful than Na3A1F6, the modifiers SrCl2, and R.E. Cl3 are quick acting, like NaF, but less powerful, the grain refiner containing K2TiF6 and KBF4 with Ti/B=1 is more potent than that with Ti/B=5. The analytical results indicate that the mass-transfer rate for the flux injection method is much higher than that for the conventional method .
基金National Natural Science Foundation of China (No. 19889502)
文摘The pellet injection experiments for fuelling and diagnostics have been carried out on the HL-1M tokamak. The eight-pellet injector was installed on HL-1M. A reliable monitordetector and camera system was set up to take initial pellet photographs and measure the initial pellet speed and size. High fuelling efficiency of 60 % - 100 % and a density profile with a peaking factor of 1.8 - 2.0 were obtained. The maximum density close to 10^14/cm^3 in HL-1M was achieved with newly optimized combined fuelling techniques. Two typical models of pellet ablation have been utilized for simulative calculation of the ablation rates in HL-1M. In comparison with the distribution of the measured Hα emission intensity from the digital data of the CCD camera, the experimental result seems more optimistic for core fuelling than theoretical predictions by the two models. The safety factor profile q(r) has been extracted from the information provided by the CCD camera during the pellet injection. The reliability of the measured results depends mainly on the calibration of the imaging space position. Based on the calibration, the measured q-profile becomes more reasonable than those published previously for the same shot number and same photograph.
文摘1 Scope This standard specifies the classification, techni cal requirements, test methods, quality appraisal pro- cedures, packing, marking, transportation, storage, quality certificate, and construction manual of nonwater injection mix for blast furnace.
基金supported by a research grant from the Universiti Kebangsaan Malaysia (Project Code: UKM-GGPM-NBT-080-2010)
文摘An analysis is performed to study the heat transfer characteristics of steady two-dimensional boundary layer flow past a moving permeable flat plate in a nanofluid. The effects of uniform suction and injection on the flow field and heat transfer characteristics are numerically studied by using an implicit finite difference method. It is found that dual solutions exist when the plate and the free stream move in the opposite directions. The results indicate that suction delays the boundary layer separation, while injection accelerates it.
基金supported by the National Natural Science Foundation of China No.81072901the New Teacher Fund for Doctor Station,Ministry of Education,No.20120013110013+1 种基金grants from the Nautical Traditional Chinese Medicine Discipline,No.522/0100604054grants from the Nautical Traditional Chinese Medicine Collaborative Innovation Center,No.522/0100604299
文摘Tongluojiunao (TLJN) is an herbal medicine consisting of two main components, geniposide and ginsenoside Rg1. TLJN has been shown to protect primary cultured hippocampal neurons. How-ever, its mechanism of action remains unclear. In the present study, primary cultured hippocampal neurons treated with Aβ1-42 (10 μmol/L) signiifcantly increased the release of lactate dehydroge-nase, which was markedly reduced by TLJN (2 μL/mL), speciifcally by the component geniposide (26 μmol/L), but not ginsenoside Rg1 (2.5 μmol/L). hTe estrogen receptor inhibitor, ICI182780 (1 μmol/L), did not block TLJN-or geniposide-mediated decrease of lactate dehydrogenase under Aβ1-42-exposed conditions. However, the phosphatidyl inositol 3-kinase or mitogen-activated protein kinase pathway inhibitor, LY294002 (50 μmol/L) or U0126 (10 μmol/L), respectively blo cked the decrease of lactate dehydrogenase mediated by TLJN or geniposide. hTerefore, these results suggest that the non-classical estrogen pathway (i.e., phosphatidyl inositol 3-kinase or mitogen-activated protein kinase) is involved in the neuroprotective effect of TLJN, speciifcally its component, geniposide, against Aβ1-42-mediated cell death in primary cultured hippocampal neurons.
文摘Objective: To explore the effect of ligustrazine injection (LI) on serum levels of interleukin-4 (IL-4) and interferon- -γ (IFN- -γ ) in patients with bronchial asthma and determine the mechanism of action of LI in preventing and treating asthma. Methods: Sixty-eight patients with mild or moderate bronchial asthma were assigned to two groups equally according to their sequence number, odd or even. The conventional treatment was administered to both groups, and LI was given to the treatment group by ultrasonic spray inhalation twice a day but not to the control group. The therapeutic course for all was 2 weeks. Further, 30 healthy subjects who had no history of smoking were enrolled as the normal control group. The clinical condition scores, frequency of attacks and dosage of Terbutaline inhaled were scored and recorded on the first day of hospitalization (before treatment) and after treatment. At the same time, the indexes of lung function, including forced expiratory volume in one second (FEV1), forced expiratory volume in one second to forced vital capacity ratio (FEV1%) and the peak expiratory flow (PEF) were determined before treatment. The levels of IL-4 and IFN--γ in peripheral blood were detected by ELISA before and after treatment, and compared with that of the healthy control group. Results: After treatment, the clinical condition scores were found to be lower, indexes of lung function were elevated, but serum level of IL-4 and ratio of IL-4/IFN-γwere reduced in both groups, showing significant differences as compared to those before treatment (P〈0.05). However, the changes in all the indexes were more significant in the treatment group than in the control group, also showing statistical significance (P〈0.05). No significant difference was revealed when IFN--γ levels were compared before and after treatment in both groups, though a lowering trend could be seen, significant difference could not be found in the comparison of IFN--γ levels between groups after treatment (P〉0.05). Conclusion: LI shows good clinical effect in treating bronchial asthma, and its mechanism might be related to the suppression of the synthesis of IL-4, thus leading to the inhibition of TH2 cell subset preponderant response and immune equilibrium regulation.
基金supported by the Ministry of Science and Innovation of Spain under the grant PID2019-109371GB-I00,and by the Junta de Castilla y Leon(VA044G19 y VA2020P20)the funding received from the pre-doctoral grant(2018/12504)with cofinancing from the Government of Castilla-La Mancha and the European Union through the European Social Fund.
文摘Fused filament fabrication(FFF)is one of the additive manufacturing processes which has gained more interest because of its simplicity and low-cost.This technology is similar to the conventional metal injection moulding(MIM)process,consisting of the feedstock preparation of metal powder and polymer binders,followed by layer-by-layer 3D printing(FFF)or injection(MIM)to create green parts and,finally,debinding and sintering.Moreover,both technologies provide near-dense parts.This work presents an in-depth study of the processing method’s influence.The porosity,microstructure,hardness,corrosion,and tribocorrosion behaviour are compared for 17-4 PH SS samples processed from powder by additive manufacturing using FFF and MIM,as well as conventional powder metallurgy(PM)samples.MIM samples exhibited the highest macro and microhardness,while corrosion behaviour was similar for both MIM and FFF samples,but superior in comparison to conventional PM samples.However,the FFF-as fabricated samples displayed a significant improvement in tribocorrosion resistance that could be explained by the higher proportion of delta ferrite and retained austenite in their microstructure.
基金Supported by Key Medical Research Subject of Hebei Province(the Protective Effect and Mechanism of Xuebijing Injection on Cardiac Function in Patients With Sepsis,No.ZD20140379)
文摘OBJECTIVE: To investigate the protective effect and possible mechanism of Xuebijing Injection on myocardial injury in patients with sepsis, and to evaluate its prognostic implications.METHODS: Patients with septic myocardial injury were recruited, and were randomly divided into two groups: treatment group and control group. All patients in two groups received conventional cluster treatment, the patients in treatment group additional received Xuebijing injection dissolved in0.9% sodium chloride injection, and the patients in control group received the same amount of 0.9%sodium chloride injection. At the beginning of treatment and 3, 7 and 10-day after treatment, lab-oratory indicators of cardiac troponin Ⅰ(cTnI),N-terminal pro B-type natriuretic peptide(NT-pro BNP) and procalcitonin(PCT) were respectively tested in venous blood. The patient's length of stay in Intensive Care Unit(ICU) and the mortality in 28 days were recorded.RESULTS: At 3, 7 and 10-day after treatment, the improvements of c Tn I, NT-pro BNP and PCT in treatment group were better than those in control group, and the differences were statistically significant(P < 0.05). The mortality of treatment group in28 days was not significantly different from that of control group(P > 0.05). The ICU length of stay of treatment group was shorter than that of control group(P > 0.05).CONCLUSION: Xuebijing injection could improve the levels of c Tn I, NT-pro BNP and PCT in patients with septic myocardial injury.and it had a protective effect on myocardial injury.
文摘In this paper, the desulphurisation of high sulphur low-rank coal is proposed as a raw material for pulverised coal injection technology. Therefore, the influence of oxidant linear velocity and the size of the coal grain was investigated in a fluidised bed. The hydrodynamic parameters of the fluidised bed including: porosity, Sherwood criterion (diffusion Nusselt number), and mass transfer coefficient (external surface) were calculated. Furthermore, the study examined the effects of intensity and efficiency on the desulphurised coal properties; organic matter, ash, and volatile matter contents. The key changes during the conversion of pyritic sulphur and coal organic matter were subsequently examined. The results showed that the sulphur content (St^d 3.16 wt%) of the low-rank coal, was transformed to (St^d 〈 1.5 wt%) after desulfurization. Other enhanced properties were: V^daf ≤ 38.0 wt%; A^d 〈 10.0 wt%, now suitable for pulverised coal injection technology.
基金financial support from the Major Subject of National Science and Technology (2011ZX05032-001)the Fundamental Research Funds for the Central Universities(NO.11CX06022A)
文摘The diffusion coefficient of natural gas in foamy oil is one of the key parameters to evaluate the feasibility of gas injection for enhanced oil recovery in foamy oil reservoirs. In this paper, a PVT cell was used to measure diffusion coefficients of natural gas in Venezuela foamy oil at high pressures, and a new method for deter- mining the diffusion coefficient in the foamy oil was de- veloped on the basis of experimental data. The effects of pressure and the types of the liquid phase on the diffusion coefficient of the natural gas were discussed. The results indicate that the diffusion coefficients of natural gas in foamy oil, saturated oil, and dead oil increase linearly with increasing pressure. The diffusion coefficient of natural gas in the foamy oil at 20 MPa was 2.93 times larger than that at 8.65 MPa. The diffusion coefficient of the natural gas in dead oil was 3.02 and 4.02 times than that of the natural gas in saturated oil and foamy oil when the pressure was 20 MPa. However, the gas content of foamy oil was 16.9 times higher than that of dead oil when the dissolution time and pressure were 20 MPa and 35.22 h, respectively.
基金supported by the National Standard Research of Traditional Chinese Medicine for Reduning Injection(ZYBZHC-JS-31)National Standard Research of Traditional Chinese Medicine for Guzhi Fuling Capsule(ZYBZH-C-JS-28)grant from the National Natural Science Foundation of China(No.81602984)。
文摘Objective:To study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning(RDN)Injection.Methods:In this study,the active fraction of RDN Injection was screened by the LPS-induced mouse endotoxin shock model.The chemical constituents were isolated by chromatography on HP-20 macroporous adsorptive resins,silica gel,ODS columns and reverse phase MPLC and HPLC repeatedly,and their structures were elucidated based on spectroscopic analysis(HR-ESI-MS,NMR,ECD)and chemical methods.Meanwhile,we evaluated the anti-inflammatory activities of the isolates by measuring their inhibitory effects on TNF-αproduction in LPS stimulated RAW 264.7 macrophages.Results:The 95%ethanol eluate of RDN Injection by the macroporous adsorption resin column was proved to be the antipyretic and anti-inflammatory active fraction of this injection.A novel iridoid,named jasminoide A(1),and a new guaiane sesquiterpenoid,named(1 R,7 R,8 S,10 R)-7,8,11-trihydroxy-4-guaien-3-one(2),were isolated from Reduning injection,and compound 2 can inhibit TNF-αproduction with IC50 values of 72.24μmol/L.Conclusion:In this study,two new terpenoids were isolated from Reduning Injection,and compound 2 showed inhibitory activity against LPS-activated TNF-αproduction in RAW 264.7 cells in vitro.
文摘To solve the problems caused by military software security issues,this paper firstly introduces a new software fault injection technique,namely main static fault injection method:program mutation.And then source code for testing this algorithm is put forward.On this basis buffer overflow testing based on program mutation is conducted.Finally several military software source codes for buffer overflow testing are tested using deficiency tracking system(DTS)tool,Experimental results show the effectiveness of the proposed algorithm.
文摘In this thesis a reviwe is presented for the advances of catalytic spec trophotometric method, three new catalytic reaction systems were presented, and its rela live catalytic spectrophotometric methods have been established by flow injection tech nique. Particulary the relative chemometric methods and theories were used in the field of kinetic - catalytic analysis, in order to solve the problems of multicomponents analysis.