Diabetes mellitus(DM)has emerged as a serious public health concern,due to the high morbidity and mortality resulted from its complications,such as diabetic nephropathy,diabetic cardiovascular complication,and diabeti...Diabetes mellitus(DM)has emerged as a serious public health concern,due to the high morbidity and mortality resulted from its complications,such as diabetic nephropathy,diabetic cardiovascular complication,and diabetic neuropathy,etc.In this study,we investigated the beneficial effects of sepia ink melanin(SIM)on hyperglycaemia and the restoration of diabetic symptoms in streptozotocin(STZ)-induced diabetic model mice.At first,the normal experimental mice were performed with intraperitoneal injection of STZ(40 mg(kg BW)^(−1))(BW,body weight)to attain diabetes and then were treated with different concentrations of SIM(120,240 and 480 mg(kg BW)^(−1))for four weeks.After treatment,significant decrease in gluconeogenesis were determined,accompanied by a notable increase in both glycolysis and oxidative enzyme activities in SIM-treated groups,such as liver marker enzymes in the serum and key antioxidant enzymes in liver.qPCR results revealed the transcriptional alterations in SIM-treated groups.SIM exposure increased the expression levels of several genes related to insulin transduction and PI3K/Akt pathway,including PI3K,Akt,Irs-2,and InsR.Meanwhile,expression levels of Dicarbonyl/l-xylulose reductase(Dcxr)and UDP-glucose dehydrogenase(Ugdh),which are in-volved in pentose-glucuronate interconversion pathway,were also elevated in SIM-treated groups.Furthermore,histological observation results indicated that nuclear deformation and organelle dissolution were improved,thus could enhance the liver function.These results demonstrated that SIM can be effective in ameliorating diabetic symptoms and improving disease management for diabetic patients.展开更多
探究曼氏无针乌贼墨汁黑色素(Melanin from Sepiella Maindroni Ink,MSMI)对免疫低下小鼠的调节作用。SPF级ICR小鼠60只,随机平均分为6组,其中5组(模型组,阳性对照组,墨汁黑色素低、中,高剂量组)用氢化可的松造免疫低下模型,另外一组为...探究曼氏无针乌贼墨汁黑色素(Melanin from Sepiella Maindroni Ink,MSMI)对免疫低下小鼠的调节作用。SPF级ICR小鼠60只,随机平均分为6组,其中5组(模型组,阳性对照组,墨汁黑色素低、中,高剂量组)用氢化可的松造免疫低下模型,另外一组为空白对照组。连续灌胃不同剂量的黑色素10 d,从免疫学水平评价曼氏无针乌贼墨汁黑色素的免疫调节效应。结果表明,曼氏无针乌贼墨汁黑色素(MSMI)能显著提高免疫低下小鼠的肝、脾指数(P<0.01,P<0.01),而且不同灌胃剂量的黑色素对巨噬细胞的吞噬率和吞噬能力的提高也有显著差异(P<0.05,P<0.01),曼氏无针乌贼墨汁黑色素(MSMI)也能够通过调节抗氧化体系的稳态增强机体免疫力。可见曼氏无针乌贼墨汁黑色素(MSMI)可以增强免疫器官的调节能力,尤以高剂量组最为显著。展开更多
基金supported by the Natural Science Foundation of Zhejiang Province(No.LY18C190006)sponsored by K.C.Wong Magna Fund in Ningbo University.
文摘Diabetes mellitus(DM)has emerged as a serious public health concern,due to the high morbidity and mortality resulted from its complications,such as diabetic nephropathy,diabetic cardiovascular complication,and diabetic neuropathy,etc.In this study,we investigated the beneficial effects of sepia ink melanin(SIM)on hyperglycaemia and the restoration of diabetic symptoms in streptozotocin(STZ)-induced diabetic model mice.At first,the normal experimental mice were performed with intraperitoneal injection of STZ(40 mg(kg BW)^(−1))(BW,body weight)to attain diabetes and then were treated with different concentrations of SIM(120,240 and 480 mg(kg BW)^(−1))for four weeks.After treatment,significant decrease in gluconeogenesis were determined,accompanied by a notable increase in both glycolysis and oxidative enzyme activities in SIM-treated groups,such as liver marker enzymes in the serum and key antioxidant enzymes in liver.qPCR results revealed the transcriptional alterations in SIM-treated groups.SIM exposure increased the expression levels of several genes related to insulin transduction and PI3K/Akt pathway,including PI3K,Akt,Irs-2,and InsR.Meanwhile,expression levels of Dicarbonyl/l-xylulose reductase(Dcxr)and UDP-glucose dehydrogenase(Ugdh),which are in-volved in pentose-glucuronate interconversion pathway,were also elevated in SIM-treated groups.Furthermore,histological observation results indicated that nuclear deformation and organelle dissolution were improved,thus could enhance the liver function.These results demonstrated that SIM can be effective in ameliorating diabetic symptoms and improving disease management for diabetic patients.
