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Changes in Inositol Phosphates in Low Phytic Acid Field Pea (<i>Pisum sativum</i>L.) Lines during Germination and in Response to Fertilization
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作者 Dil Thavarajah Pushparajah Thavarajah +4 位作者 Darshika Amarakoon Abby Fenlason Casey R. Johnson Phil Knutson Thomas D. Warkentin 《American Journal of Plant Sciences》 2013年第2期251-256,共6页
Inositol phosphates are the main form of phosphorous (P) storage in legume seeds. Mutants low in inositol hexaphosphate (IP6), also known as phytic acid (PA), have been developed to increase iron (Fe) bioavailability ... Inositol phosphates are the main form of phosphorous (P) storage in legume seeds. Mutants low in inositol hexaphosphate (IP6), also known as phytic acid (PA), have been developed to increase iron (Fe) bioavailability and reduce P waste to the environment. The objectives of this study were to determine 1) inositol-P form changes during germination, and 2) the effect of P fertilizer application on seed PA, total P, and Fe concentration of three field pea (Pisum sativum L.) cultivars and two low-PA lines grown under greenhouse conditions. Low-PA field pea lines clearly had lower PA (1.3 - 1.4 mg·g-1) than cultivars (3.1 - 3.7 mg·g-1). Phytic acid concentration in both cultivars and low-PA lines decreased during germination, but tended to increase seven days after germination. Levels of inositol-3-phosphate-phosphate (IP3-P;0.6 mg·g-1) and inorganic P (1.8 - 2.0 mg·g-1) were higher in low-PA lines than in the field pea cultivars. Reduction of PA in low-PA line seeds may reduce seed Fe and total P concentrations, as levels in the low-PA lines (37 - 42 mg·kg-1 Fe;4003 - 4473 mg·kg-1 total P) were typically less than in field pea cultivars (37 - 55 mg·kg-1 Fe;3208 - 4985 mg·kg-1 total P) at different P fertilizer rates. Overall, IP3 is the major form of P present in low-PA field pea lines during germination;however IP6 is the major form of P present in field pea cultivars. Therefore, low-PA field pea lines could be a potential solution to increase Fe bioavailability, feed P utilization, and reduce P waste to the environment. 展开更多
关键词 Phytic ACID inositol-3-Phosphate Low Phytic ACID mutantS Field PEAS Iron Phosphorus
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银耳的诱变与营养缺陷型菌株的筛选 被引量:3
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作者 谢宝贵 朱虎 +2 位作者 江玉姬 饶永斌 郑金贵 《江西农业大学学报》 CAS CSCD 2004年第2期246-249,共4页
实验结果表明,银耳酵母状分生孢子对紫外线的效应为多靶单击类型,而对60Coγ射线的效应为单靶单击。紫外线诱变获得2个营养缺陷型菌株,其中1株T811经鉴定是肌醇缺陷型。突变体T811的生长迟缓期比野生型多3d,生长速度较慢。同时还提出了... 实验结果表明,银耳酵母状分生孢子对紫外线的效应为多靶单击类型,而对60Coγ射线的效应为单靶单击。紫外线诱变获得2个营养缺陷型菌株,其中1株T811经鉴定是肌醇缺陷型。突变体T811的生长迟缓期比野生型多3d,生长速度较慢。同时还提出了该突变体的应用前景。 展开更多
关键词 银耳 诱变 营养缺陷型菌株 筛选 紫外线 Γ射线 生物反应器 分生孢子 突变体 基因工程
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