INTS11通过介导CDK2和CYCLIND1的转录促进牛成肌细胞增殖

旨在验证牛INTS11基因在成肌细胞中的功能作用,通过生物信息学分析探讨INTS11的CDS区序列及其编码蛋白的特征,并使用不同分子试验技术验证其在牛成肌细胞增殖过程中发挥的作用。本研究对牛INTS11基因序列与其他物种进行同源性对比并构建生物进化树,对其编码蛋白进行理化性质和功能结构分析以及亚细胞定位。并以体外分离培养的3月龄健康胎牛成肌细胞为试验材料,克隆成肌细胞中INTS11的全部CDS区序列,构建INTS11的过表达载体并设计基因的干扰序列,通过CCK8、Edu、RT-qPCR等技术探究其对成肌细胞增殖的影响。生物信息分析结果表明,CDS区序列全长为1800 bp,具有MBL-foldmetallo-hydro和β-CASP基序两个功能结构域,属于非跨膜蛋白,且细胞定位主要分布于细胞质。在成肌细胞中转染pcDNA3.1-INTS11质粒,结果显示在72~96 h时试验组的成肌细胞比对照组的增殖速度显著增加(P<0.05);Edu试验发现阳性细胞数目显著增加(P<0.05);RT-qPCR结果表明增殖标志因子CDK2、CYCLIND1的mRNA表达水平与对照组相比显著上升(P<0.05)。转入干扰序列后,细胞增殖速度显著下降(P<0.05),同时增殖标志因子CDK2、cyclin D1的mRNA表达水平与对照组相比显著下降(P<0.05)。本研究预测了INTS11在家养动物中的保守性,并且INTS11蛋白具有两个功能结构域,发现其通过介导CDK2、CYCLIND1的mRNA转录促进牛成肌细胞增殖,其结果完善了调控骨骼肌的基因网络,为后序探讨调控骨骼肌生长机制提供理论支持。

INTS10对HCC细胞周期、凋亡、生长和迁移能力的影响

为了探究整合因子复合物亚基10(integrator complex subunits 10,INTS10)对人肝细胞癌(hepatocellular carcinoma,HCC)细胞周期、凋亡、生长和迁移能力的影响及其潜在的分子作用机制,利用慢病毒感染法获得稳定过表达或敲低INTS10的HCC细胞系,采用qRT-PCR和Western blotting检测INTS10 mRNA和蛋白表达水平,接着采用CCK-8法、克隆形成和BrdU实验检测细胞生长情况,采用Transwell小室实验检测细胞迁移能力,采用流式分析术检测细胞的周期和凋亡.结果显示:过表达INTS10可显著抑制HCC细胞的凋亡、生长和迁移能力,促进G1期细胞数量的增加,而敲低INTS10则呈现相反的表型.通过通路富集分析发现,周期相关通路被显著富集,过表达INTS10后,CDC25A和CDK4的mRNA和蛋白质水平显著减少,而CDKN1A的水平显著增加,敲低INTS10则呈现相反趋势.综上,本研究初步揭示了INTS10在HCC细胞中可能通过影响G1/S期相关蛋白质的表达而发挥抑癌基因的功能,为下一步更为深入的功能和机制研究提供了基础.

A 1.25Gb/s InP-Based Vertical Monolithic Integration of an MQW Laser Diode and an HBT Driver witha Lateral Buffer Mes a Structure

A novel fabrication process related to a smoothly wet chemical etching profile o f InP-based epitaxial layers in the crystal direction of [01for an InP-based monol ithic vertically integrated transmitter with an MQW laser diode and a heterojunction bipolar tran sistors driver circuit is described.A clear eye output diagram via an O/E converter is demonstrat ed und er a 1.25Gb/s non-return-zero pseudorandom code with a pattern length of 2 the integrated transmitter has a power dissipation of about 120mW with an optical output of 2dBm.

国际化学SCI期刊导航《International Journal of Mass Spectrometry》

《International Journal of Mass Spectrometry》（缩写为 INT J MASS SPECTROM（ ISSN：1387-3806）是目前国际公认的质谱学高水平学术期刊之一，Elsevier Science 公司出版。该刊创刊于1968年，早期刊名《International Journal of Mass Spectrometry and Ion Physics》，1983年10月更名为《International Journal of Mass Spectrometry and Ion Processes》，1998年6月更名为《International Journal of Mass Spectrometry》，现为半月刊。

Combined transjugular intrahepatic portosystemic shunt and other interventions for hepatocellular carcinoma with portal hypertension

AIM: To evaluate combination transjugular intrahepatic portosystemic shunt(TIPS) and other interventions for hepatocellular carcinoma(HCC) and portal hypertension.METHODS: Two hundred and sixty-one patients with HCC and portal hypertension underwent TIPS combined with other interventional treatments(transarterial chemoembolization/transarterial embolization,radiofrequency ablation,hepatic arterio-portal fistulas embolization,and splenic artery embolization) from January 1997 to January 2010 at Beijing Shijitan Hospital. Two hundred and nine patients(121 male and 88 female,aged 25-69 years,mean 48.3 ± 12.5 years) with complete clinical data were recruited. We evaluated the safety of the procedure(procedurerelated death and serious complications),change of portal vein pressure before and after TIPS,symptom relief [e.g.,ascites,hydrothorax,esophageal gastricfundus variceal bleeding(EGVB)],cumulative rates of survival,and distributary channel restenosis. The characteristics of the patients surviving ≥ 5 and < 5 years were also analyzed.RESULTS: The portosystemic pressure was decreased from 29.0 ± 4.1 mm Hg before TIPS to 18.1 ± 2.9 mm Hg after TIPS(t = 69.32,P < 0.05). Portosystemic pressure was decreased and portal hypertension symptoms were ameliorated. During the 5 year followup,the total recurrence rate of resistant ascites or hydrothorax was 7.2%(15/209); 36.8%(77/209) for EGVB; and 39.2%(82/209) for hepatic encephalopathy. The cumulative rates of distributary channel restenosis at 1,2,3,4,and 5 years were 17.2%(36/209),29.7%(62/209),36.8%(77/209),45.5%(95/209) and 58.4%(122/209),respectively. No procedure-related deaths and serious complications(e.g.,abdominal bleeding,hepatic failure,and distant metastasis) occurred. Moreover,Child-Pugh score,portal vein tumor thrombosis,lesion diameter,hepatic arterio-portal fistulas,HCC diagnosed before or after TIPS,stent type,hepatic encephalopathy,and type of other interventional treatments were related to 5 year survival after comparing patient characteristics.CONCLUSION: TIPS combined with other interventional treatments seems to be safe and efficacious in patients with HCC and portal hypertension.

Enteric glial cells and their role in the intestinal epithelial barrier

The intestinal epithelium constitutes a physical and functional barrier between the external environment and the host organism. It is formed by a continuous monolayer of intestinal epithelial cells maintained together by intercellular junctional complex, limiting access of pathogens, toxins and xenobiotics to host tissues. Once this barrier integrity is disrupted, inflammatory disorders and tissue injury are initiated and perpetuated. Beneath the intestinal epithelial cells lies a population of astrocyte-like cells that are known as enteric glia. The morphological characteristics and expression markers of these enteric glia cells were identical to the astrocytes of the central nervous system. In the past few years, enteric glia have been demonstrated to have a trophic and supporting relationship with intestinal epithelial cells. Enteric glia lesions and/or functional defects can be involved in the barrier dysfunction. Besides, factors secreted by enteric glia are important for the regulation of gut barrier function. Moreover, enteric glia have an important impact on epithelial cell transcriptome and induce a shift in epithelial cell phenotype towards increased cell adhesion and cell differentiation.Enteric glia can also preserve epithelial barrier against intestinal bacteria insult. In this review, we will describe the current body of evidence supporting functional roles of enteric glia on intestinal barrier.

1994开启中国INTERNET元年的先驱者们

美国副总统戈尔早在1991年任参议员时便提出了划时代的"信息高速公路"法案,但一直未有实质性的进展。克林顿上台后将这一计划视为重振美国经济的重要组成部分,在1993年2月与副总统戈尔一起在硅谷访问时强调,建设"信息高速公路"是新政府技术政策的中心,将被置于白宫科技议事日程的首要位置。同年9月,副总统戈尔和商务部长布朗正式宣布了实施美国国家信息基础设施的"行动日程"计划,揭开了美国兴建"信息高速公路"的序幕,预示着全球网络信息化时代的到来。正是由于一批科学家和教授等精英阶层的推动,中国也抓住了机遇,紧跟时代的脚步,于1994年正式联入国际Internet,并在1995年开启了中国Internet的商业化、大众化,从此中国Internet进入了蓬勃发展的时代。

Optimal design of feeding system in steel casting by constrained optimization algorithms based on InteCAST

The traditional foundry industry has developed rapidly in recently years due to advancements in computer technology. Modifying and designing the feeding system has become more convenient with the help of the casting software, Inte CAST. A common method of designing a feeding system is to first design the initial systems, run simulations with casting software, analyze the feedback, and then redesign. In this work, genetic, fruit fly, and interior point optimizer(IPOPT) algorithms were introduced to guide the optimal riser design for the feeding system. The results calculated by the three optimal algorithms indicate that the riser volume has a weak relationship with the modulus constraint; while it has a close relationship with the volume constraint. Based on the convergence rate, the fruit fly algorithm was obviously faster than the genetic algorithm. The optimized riser was also applied during casting, and was simulated using Inte CAST. The numerical simulation results reveal that with the same riser volume, the riser optimized by the genetic and fruit fly algorithms has a similar improvement on casting shrinkage. The IPOPT algorithm has the advantage of causing the smallest shrinkage porosities, compared to those of the genetic and fruit fly algorithms, which were almost the same.

interleukin 28B genetic polymorphism and hepatitis B virus infection

Interleukin (IL) 28B genetic polymorphism is significantly associated with the sustained virological response rate in patients with chronic hepatitis C treated with pegylated interferon-&#x003b1; (PEG-IFN) plus ribavirin and with spontaneous hepatitis C virus clearance. However, a consensus on the relationship between IL28B genetic polymorphism and the favorable outcome of chronic hepatitis B virus infection defined by hepatitis B e antigen seroconversion, and/or hepatitis B surface antigen seroclearance in patients treated with interferon or PEG-IFN has not been reached. Several reports failed to show a positive association, while some studies demonstrated a positive association in certain subject settings. More prospective studies including large cohorts are needed to determine the possible association between IL28B genetic polymorphism and the outcome of interferon or PEG-IFN treatment for chronic hepatitis B.

Hepatobiliary & Pancreatic Diseases International(HBPD INT)

Hepatobiliary & Pancreatic Diseases International (HBPD INT) is a journal published bimonthly in English language by the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. We welcome original

Efficient thermal analysis method for large scale compound semiconductor integrated circuits based on heterojunction bipolar transistor

In this paper, an efficient thermal analysis method is presented for large scale compound semiconductor integrated circuits based on a heterojunction bipolar transistor with considering the change of thermal conductivity with temperature.The influence caused by the thermal conductivity can be equivalent to the increment of the local temperature surrounding the individual device. The junction temperature for each device can be efficiently calculated by the combination of the semianalytic temperature distribution function and the iteration of local temperature with high accuracy, providing a temperature distribution for a full chip. Applying this method to the InP frequency divider chip and the GaAs analog to digital converter chip, the computational results well agree with the results from the simulator COMSOL and the infrared thermal imager respectively. The proposed method can also be applied to thermal analysis in various kinds of semiconductor integrated circuits.

Using Communicative Language Teaching of English in Spoken English for the Major of International Economics and Trade

Enhancing the ability of oral communication is the general trend of language teaching in China. Through analyzing the characteristics of International Economics and Trade Major, this paper identifies and analyses contextual factors in English Language Teaching in the major, and discusses the practices of communicative language teaching which could be adapted to this context, in which learners are undergraduates of International Economics and Trade, then the importance and difficulties of the teaching approach has been highlighted.

Hepatobiliary & Pancreatic Diseases International (HBPD Int)

Hepatobiliary & Pancreatic Diseases International(HBPD Int)is a journal published quarterly inEnglish language by the First Affiliated Hospital,Zhejiang University School of Medicine,Hang-zhou,China.We welcome original articles fromany part of the world;no charge is made for re-view.The papers are reviewed by members of theEditorial Board and our international panel of ex-pert peer reviewers,then either accepted for publi-cation or rejected by the Editor.Please send allsubmissions to:The EditorHepatobiliary & Pancreatic Diseases International(HBPD Int),First Affiliated Hospital,ZhejiangUniversity School of Medicine,79 Qingchun Road,

Hepatobiliary & Pancreatic Diseases International (HBPD Int)

Hepatobiliary & Pancreatic Diseases International(HBPD Int) is a journal published quarterly inEnglish language by the First Affiliated Hospital,Zhejiang University School of Medicine, Hang-zhou, China. We welcome original articles fromany part of the world; no charge is made for re-view. The papers are reviewed by members of theEditorial Board and our international panel of ex-pert peer reviewers, then either accepted for publi-cation or rejected by the Editor. Please send allsubmissions to:

Hepatobiliary & Pancreatic Diseases International (HBPD Int)

Hepatobiliary & Pancreatic Diseases International(HBPD Int) is a journal published quarterly inEnglish language by the First Affiliated Hospital,Zhejiang University School of Medicine, Hang-zhou, China. We welcome original articles fromany part of the world; no charge is made for re-view. The papers are reviewed by members of theEditorial Board and our international panel of ex-pert peer reviewers, then either accepted for publi-cation or rejected by the Editor. Please send allsubmissions to:The EditorHopatobiliary & Pancreatic Diseases International(HBPD Int), First Affiliated Hospital, Zhejiang

Hepatobiliary & Pancreatic Diseases International (HBPD Int)

Aims and ScopeHBPD Int publishes peer-reviewed original papers,reviews and editorials concerned with clinical prac-tice and research in the fields of hepatobiliary andpancreatic diseases. Papers cover the medical,surgical, radiological, pathological, biochemical,physiological and historical aspects of the subjectareas under the headings Liver, Biliary, Pancreas,Transplantation, Research, Special Reports, Edi-torials, Reviews, Brief Communications and CaseReports. The journal also deals with the basic sci-ences and experimental work. All submitted papersare reviewed by at least two referees expert in the

Hepatobiliary & Pancreatic Diseases International (HBPD Int)

Aims and ScopeHBPD Int publishes peer-reviewed original papers,reviews and editorials concerned with clinical prac-tice and research in the fields of hepatobiliary andpancreatic diseases. Papers cover the medical,surgical, radiological, pathological, biochemical,physiological and historical aspects of the subjectareas under the headings Liver, Biliary, Pancreas,Transplantation, Research, Special Reports, Edi-torials, Reviews, Brief Communications and CaseReports. The journal also deals with the basic sci-ences and experimental work. All submitted papersare reviewed by at least two referees expert in thefield of the submitted paper. For detailed instruc-tions concerning the submission of manuscripts,

Hepatobiliary & Pancreatic Diseases International (HBPD Int)

Hepatobiliary & Pancreatic Diseases International(HBPD Int) is a journal published quarterly inEnglish language by the First Affiliated Hospital,Zhejiang University School of Medicine, Hang-zhou, China. We welcome original articles fromany part of the world; no charge is made for re-view. The papers are reviewed by members of theEditorial Board and our international panel of ex-pert peer reviewers, then either accepted for publi-cation or rejected by the Editor. Please send allsubmissions to:

Hepatobiliary & Pancreatic Diseases International (HBPD Int)

Aims and ScopeHBPD Int publishes peer-reviewed original papers,reviews and editorials concerned with clinical prac-tice and research in the fields of hepatobiliary andpancreatic diseases. Papers cover the medical,surgical, radiological, pathological, biochemical,physiological and historical aspects of the subjectareas under the headings Liver, Biliary, Pancreas,Transplantation, Research, Special Reports, Edi-torias, Reviews , Brief Communications and CaseReports. The journal also deals with the basic sci-ences and experimental work. All submitted papersare reviewed by at least two referees expert in the

INT对篮球运动员下肢爆发力的影响研究

目的:通过同一项群训练的迁移理论,验证INT对篮球运动员的下肢爆发力的影响。研究方法:选择20名平均年龄18岁的大学男子篮球运动员作为实验对象,分为实验组(EG)和对照组(CG),对EG受试者进行8周,每周3次,每次90 min;CG受试者进行8周传统力量训练,每周3次,每次90 min,在实验干预前后分别对受试者下肢爆发力进行测试。研究结果:训练干预后,EG与CG受试者相比,助跑垂直纵跳、垂直纵跳、单脚侧跳和立定跳远成绩均有显著性提高(P<0.05)。研究结论:INT训练能够对篮球运动员的下肢爆发力产生积极的影响。建议:在球类实践或训练过程中,可以合理利用抗阻训练、超等长训练、动态稳定训练、速度训练和灵敏训练等与专项技术运动相结合,提升在比赛中的专项动作效率,使INT训练的综合效果能直接运用到比赛所需的技战术动作模式中,为运动员训练效果的专项化迁移奠定扎实基础。