Evaluation on the Morphology and Membrane Integrity of Immotile Human Sperm

Objective To determine the membrane integrity in the head and tail regions of individual spermatozoon, and observe sperm morphology for samples with totally immotile sperm. Methods Ten infertile men with immotile sperm were enrolled into this study （group A）. The membrane integrity in the head and tail regions of individual spermatozoon of immotile sperm was examined by using the combined hypo-osmotic swelling-eosin Y exclusion test （HOS-EY test）. Sperm morphology was observed by light, scanning and transmission electron microscopy. Ten semen samples from normospermic donors were used as the control （group B）. Results The percentage of sperm with intact both head and tail membranes in group A was significantly lower than that in group B （P〈0.01）, whereas the value of sperm with defective head membrane but intact tail membrane in group A was significantly higher than that in group B （P〈0.01）. Abnormal sperm morphology in group A had a high incidence, and immotile sperm with viability and normal morphology could be observed in some cases. Most sperm had multiple ultrastructural defects. Conclussion Some immotile sperm had intact tail membrane but defective head membrane. Immotile sperm with viability and normal morphology could exist in some cases though abnormal sperm were in a great proportion. Carefully evaluating immotile sperm membrane integrity and morphology should benefit the treatment of patients with immotile sperm.

ZTE Wins Best Integrated IMS Solution Award

25 May 2012, Shenzhen- ZTE Corporation has been awarded the Best Integrated IMS Solution Award at IMS World Forum 2012 in Madrid. ZTE was awarded for its IMS-based legacy voice service （PSTN/ISDN） emulation solution, which has been deployed in 80 commercial networks worldwide.

Integrity Assessment of Pipe System in a Full-Scale Membrane Water Treatment Plant

The pipe system roles as a main bridge between membrane modules and pumps in membrane water treatment plants. Membrane operation modes generally consist of filtration and backwash processes in a normal mode, a pressure decay test as an integrity test and a chemical circulation through pipe system in a cleaning mode. Thus factors effecting on membrane performance should be sufficiently considered before design and operation. This study evaluated flow analysis for vibration diagnostic and evaluation of the fatigue lifetime in the microfiltration system applied for a drinking water treatment plant. Vibration of main membrane pipelines was measured to identify the source of vibration. Also natural frequency and fluid dynamics was calculated by computational fluid dynamics. It showed that maximum magnitude frequencies were at 12 Hz and 22 Hz, respectively at water and air pipeline during filtration and backwash. Backwash process caused mainly vibration on the backwash water pipe. The calculated frequency from analysis of frequency response and CFD was in a good agreement with the measured frequency. Fatigue analysis showed that pipelines were getting little damage caused by vibration. Fatigue lifetime was predicted more than 15 years under the operation condition of daily filtration, and more than 27 years under the operation condition of a daily backwash mode, resulting in minor damage on the pipe lifetime.

Differential expression and regulation of integral membrane protein 2b in rat male reproductive tissues

Aim： To examine the expression and regulation of integral membrane protein 2b （Itm2b） in rat male reproductive tissues during sexual maturation and under different treatments by in situ hybridization. Methods： Testis, epididymis, and vas deferens were collected on days 1-70 to examine Itm2b expression during sexual maturation. To further examine the regulation of Itm2b, adult rats underwent surgical castration and cryptorchidism. Ethylene dimethane sulfonate and busulfan treatments were carried out to test the regulation of Itm2b after destruction of Leydig cells and germ cells. Results： In testis, Itm2b expression was moderately detected in the adluminal area of seminiferous cords on days 1-10, and detected at a low level in the spermatogonia on days 20 and 30. The Itm2b level was markedly increased in Leydig cells from day 20 to day 70. In epididymis and vas deferens, Itm2b was detected from neonate to adults, and the signal gradually increased in accordance with sexual maturation. Itm2b expression was significantly downregulated in epididymis and vas deferens of castrated rats, and strongly stimulated when castrated rats were treated with testosterone. Cryptorchidism led to a significant decline of Itm2b expression in testis and caput epididymis. Itm2b expression in epididymis and vas deferens was significantly decreased after the Leydig ceils were destroyed by ethylene dimethane sulfonate. Busulfan treatment produced no obvious change in Itm2b expression in epididymis or vas deferens. Conelusion： Our data suggested that Itm2b expression is upregulated by testosterone and might play a role in rat male reproduction.

First integrals of the axisymmetric shape equation of lipid membranes

The shape equation of lipid membranes is a fourth-order partial differential equation.Under the axisymmetric condi-tion,this equation was transformed into a second-order ordinary differential equation(ODE)by Zheng and Liu(Phys.Rev.E 482856(1993)).Here we try to further reduce this second-order ODE to a first-order ODE.First,we invert the usual process of variational calculus,that is,we construct a Lagrangian for which the ODE is the corresponding Euler-Lagrange equation.Then,we seek symmetries of this Lagrangian according to the Noether theorem.Under a certain restriction on Lie groups of the shape equation,we find that the first integral only exists when the shape equation is identical to the Will-more equation,in which case the symmetry leading to the first integral is scale invariance.We also obtain the mechanical interpretation of the first integral by using the membrane stress tensor.

Effects of Ce⁴⁺on Membrane Integrity of Rice in Seedling Hydroponic Cultures

Rare earth elements have been extensively used as micro-fertilizers for crops in China. To?understand the potential benefits or damages of Ce4+ on rice, the effects of Ce4+ on viability and membrane integrity of rice were investigated under hydroponic cultures. It has been found that the cell viability, electrical conductivity, pH changes, and concentration of malonyl dialdehyde did not change obviously when the content of Ce4+ was 0.5 mg/L, but varied markedly when the contents of Ce4+ were raised to 10 mg/L or more, indicating that the Ce4+ at higher concentrations severely affected the cell membrane permeability.

Anti-VDAC3 recombinant antibody decreased human sperm motility and membrane integrity: A potential spermicide for contraception

Objective:To express recombinant protein that comprises an important fragment of human sperm specific voltage dependent anion channel 3 (VDAC3) protein as a potential molecule for generation of antibody, which can affect sperm function, aiming at spermicide development. Methods: The produce of VDAC3 recombinant protein encoded by cDNA sequence of human VDAC3 exon 5-8, based on experimental design of VDAC3 knock-out mice study. And after the purification of various human sperm VDAC3 recombinant proteins, epitope has been predicted in our recombinant protein determined by ElliPro program. Polyclonal antibody was produced for 14 wk. Then anti-VDAC3-exon 5-8 recombinant antiserum was inoculated to human sperm. After the process, antibody VDAC3 protein in human sperm was incubation with anti-VDAC3 recombinant antibody. Finally evaluation the effect of VDAC3 antiserum to human sperm motility and plasma membrane integrity was proceeded.Results: Human VDAC3 recombinant protein was successfully over-expressed in Escherichia coli and purified by affinity chromatography method. Purified human sperm VDAC3 recombinant protein could stimulate immune response in rabbit producing an antibody against VDAC3. Anti-VDAC3 recombinant antibody recognized VDAC3 antigen in human sperm could decrease human sperm motility and membrane integrity significantly.Conclusions:Anti-VDAC3 recombinant polyclonal antibody that we produced in rabbit by ourselves could decrease sperm motility and sperm membrane integrity. The authors suggest this polyclonal antibody could be used as a candidate agent for male contraception in the future. Furthermore, the authors intend to explore the effect of this antibody into sperm function aiming at male contraceptive vaccine development.

号码携带业务对IMS网络互通影响分析

重点讨论了IMS网络在网间互通时的原则,由此引出关口局融合的概念和阶段性建议。特别就号码携带业务对IMS网络互通的影响,结合关口局融合改造的各个阶段提出了具体解决方案。

智能管理控制终端在船舶IMS和PMS系统中的应用研究

智能管理控制终端是船舶IMS综合监测系统和PMS功率管理系统的重要组成部分。文章首先介绍了智能管理控制终端的软件及硬件组成;其次,结合IMS和PMS系统介绍了智能管理控制终端的应用和主要功能,最后研究探讨了其核心功能预测和故障诊断的原理及实现方法。

IMS组网策略研究

IMS(IP多媒体子系统)作为业界公认的核心控制层融合技术,在运营商推进网络融合进程中备受关注。通过对IMS规模部署中的组网结构、与其它网络的互通、业务平台建设等方面的研究,建议运营商在IMS的组网结构上,可以依据IMS用户规模和管理机制等因素,以大区或省为单位建设统一的IMS网络;在IMS业务平台建设上,运营商可以依据现有网络情况,灵活选择利用原有业务平台或新建IMSAS来实现业务融合,同时为固网、移动用户提供业务,同时IMS还可满足运营商多媒体业务推广的需求。

基于IMS的AMI融合通信网研究

随着智能配用电网的发展,以IP多媒体系统(IP Multimedia Subsystem,IMS)为核心的融合通信网络是未来AMI智能用电通信网络发展的必然选择。文章首先介绍了高级测量体系(Advanced Metering Infrastructure,AMI)系统的概念、体系构架、网络结构和发展方向;然后介绍了基于IMS的融合通信技术;最后阐述了基于IMS的AMI融合通信网络架构,并对它的安全性进行了分析。

Portable fluorescence instrument for detecting membrane integrity in membrane bioreactor (MBR)

This study proposed the design, fabrication, and assembly of membrane integrity detection instruments in membrane bioreactors (MBR) based on fluorescence spectroscopy. Based on the PARAFAC model, we found that the peak at 280/335 nm strengthened after membrane breakage. The peak at 340/430 nm reflected the sludge concentration in the MBR and reduced the influence of internal filtration effects on detection. Therefore, we determined that the dual-LED light source excitation detection system can detect tryptophan-like substances at 280 nm (T-peak) and humic acid at 340 nm (C-peak). T-peak was identified as the core index indicating membrane integrity. Moreover, the C-peak is the reference indicator factor for a sensitive response to changes in the sludge concentration. The portable fluorescence instrument exhibited high sensitivity and good feedback accuracy compared to particle counting and turbidity detection, where the log reduction value was greater than 3.5. This overcomes the disadvantage of false alarms in particle counters and is not affected by the position of the pump system. This portable instrument provides a flexible and highly sensitive method for the assessment of industrial membrane integrity.

IMS在南方联通落地实施及未来演进探讨

IMS作为基于全IP网络的全新电信网络架构,既是电信网络和互联网融合的产物,也为传统固网改造和固移融合带来新的契机。通过分析南方联通某省固网现状,归类总结目前存在主要问题,在部署IMS整体网络架构的基础上,特别针对IP网络承载方式、IMS与现网互通实施方式、用户数据部署策略等关键点深入剖析,对比多种可实施方案并提出建议,研究和探讨了IMS在南方联通的落地实施方案,同时对IMS在未来LTE网络中实现VoLTE进行了应用探讨。

The importance of laminin at the blood-brain barrier

The blood-brain barrier is a unique property of central nervous system blood vessels that protects sensitive central nervous system cells from potentially harmful blood components.The mechanistic basis of this barrier is found at multiple levels,including the adherens and tight junction proteins that tightly bind adjacent endothelial cells and the influence of neighboring pericytes,microglia,and astrocyte endfeet.In addition,extracellular matrix components of the vascular basement membrane play a critical role in establishing and maintaining blood-brain barrier integrity,not only by providing an adhesive substrate for blood-brain barrier cells to adhere to,but also by providing guidance cues that strongly influence vascular cell behavior.The extracellular matrix protein laminin is one of the most abundant components of the basement membrane,and several lines of evidence suggest that it plays a key role in directing blood-brain barrier behavior.In this review,we describe the basic structure of laminin and its receptors,the expression patterns of these molecules in central nervous system blood vessels and how they are altered in disease states,and most importantly,how genetic deletion of different laminin isoforms or their receptors reveals the contribution of these molecules to blood-brain barrier function and integrity.Finally,we discuss some of the important unanswered questions in the field and provide a“to-do”list of some of the critical outstanding experiments.

IMS行政交换网一体化运维支撑系统的设计

国家电网有限公司目前已完成IMS行政交换网全网建设,行政电路交换网终端用户开始逐步向IMS行政交换网迁移,现有的行政交换网运维模式已不能满足下一代行政交换网运维管理要求。文章通过对IMS行政交换网的应用现状进行调研,分析IMS交换网在业务发放、接入设备管理方面的运维需求,设计了一套适合IMS行政交换网组网架构的一体化运维系统。系统提高了IMS交换网业务发放效率、接入设备管理水平,减轻了集中运维压力,解决了目前IMS交换网运维工作中存在的关键问题。

Harvesting Microalgae Biomass Using Sulfonated Polyethersulfone(SPES)/PES Porous Membranes in Forward Osmosis Processes

This study was performed to investigate the availability of forward osmosis(FO)for microalgae harvesting using sulfonated polyethersulfone(SPES)/PES porous membranes.In FO process,porous membranes(<25.0 L m^−2 h^−1)exhibited more superior water flux than TFC FO membranes(<2.6 L m^−2 h^−1).Furthermore,the incorporation of SPES has been demonstrated to enhance membrane performance.The effects of SPES content on pore structure and separation performance were investigated.Compared with pure PES porous membranes,porous membranes with 40%SPES yielded an improved hydrophilicity and greater porosity.It exhibited two times higher water fluxes than the pure PES porous membrane.For microalgae harvesting,AL-FS mode(active layer facing the feed solution)was more favourable than AL-DS mode(active layer facing the draw solution)because less deposited microalgae on the active layer mitigate the membrane biofouling.FO operation combined with SPES/PES porous membranes is conducive to preserving microalgae cell integrity under the mild condition.In addition,FO membrane can be cleaned by a simple water rinse.Potential implications were highlighted as a sustainable method for microalgae harvesting because of no pressure input and less chemical cleaning demand.

Oblique Wave Scattering Problems Involving Vertical Porous Membranes

Oblique surface waves incident on a fixed vertical porous membrane of various geometric configurations is analyzed here.The mixed boundary value problem is modified into easily resolvable problems by using a connection.These problems are reduced to that of solving a couple of integral equations.These integral equations are solved by a one-term or a two-term Galerkin method.The method involves a basis functions consists of simple polynomials multiplied with a suitable weight functions induced by the barrier.Coefficient of reflection and total wave energy are numerically evaluated and analyzed against various wave parameters.Enhanced reflection is found for all the four barrier configurations.

面向IMS的铁通固网融合实施方案探讨

从考虑铁通固网的发展走向出发,通过介绍铁通固网的现状说明了融合的必要性。在此背景下,从技术层面提出铁通固网的融合方案及实施步骤,并介绍了实施后的效果。

BIM集成技术在2022年杭州亚运会曲棍球场馆数字化建造中的应用

杭州亚运会曲棍球场馆顶部罩棚是一个超大型骨架式膜覆盖结构。膜曲面优美生动、轻盈飘逸,罩棚结构为一个四角落地拱网壳结构。通过应用BIM集成技术,实现了该复杂曲面造型的钢网壳结构工程数字化建造,本文介绍了其数字化建造中的参数化设计、数字化制造、智能化测绘辉以及机械化安装等方面内容。

浅谈SFCS与IMS在SMT制程中的应用

电子制造业生产成本的剧烈竞争，对于以委托代工（OEM）为主的现代SMT工厂，传统的手工及口头的资讯传递与管理方式，令生产现场与管理层之间无法实时畅通，这不仅难以满足当今自动化生产和先进工艺管理要求，而且导致企业竞争力下降。因此，大家迫切地需要寻找一种全新的现场控制与智能化管理系统（参考图1），以便解决生产现场与管理层信息的滞后或脱节问题。在众多的企业管理软件系统中.