Objective： The aim of our study was to investigate the effects of （-）-epigallocatechin-3-gallate （EGCG, the major phytochemistry component in green tea） on the expression of connexin43 （Cx43） gene and detect th...Objective： The aim of our study was to investigate the effects of （-）-epigallocatechin-3-gallate （EGCG, the major phytochemistry component in green tea） on the expression of connexin43 （Cx43） gene and detect the intercellular communication of the human bladder cancer cell lines BIU-87, and explore its possible mechanisms of prevention and cure for the bladder tumor. Methods： The methyl thiazolyl tetrazolium and Annexin-V/PI double-labeled flow cytometry methods were used to observe the growth inhibitory rate （IR） and apoptosis rate （AR） of BlU-87 cells treated by EGCG at different concentrations （0, 5, 10 and 20 mg/L）, respectively. The reverse transcription-polymerase chain reaction （RT-PCR） and Western Blotting analysis were employed to detect the relative expression levels of the Cx43 mRNA and its protein. The scrape-loading fluorescence dye transfer method was used to assess the gap junction intercellular communication （GJIC） under fluorescence microscope. Results： EGCG at concentrations （10 and 20 mg/L） both could significantly inhibit the proliferation and induce the apoptosis of BIU-87 cells. The IR and AR were （15.67 ± 1.15）%, （18.33 ± 1.53）% and （42.00 ± 4.34）%, （27.33 ± 3.21）%, respectively. And compared with the control groups of 0 mg/L and 5 mg/L （P 〈 0.05）, EGCG could significantly up-regulate the expression of Cx43 mRNA and its protein and enhance the function of BIU-87 cells. The effects had the significant correlation with the dose-dependent of EGCG. Conclusion： EGCG （10, 20 mg/L） could effectively up-regulate Cx43 expression and enhance the GJIC of BlU-87 cells. The results may indicate the effects of EGCG inducing bladder tumor cells apoptosis and inhibiting its growth which provides the experimental evidence for further demonstrating the mechanism of chemical prevention and cure for the bladder tumor by EGCG.展开更多
The HAZ microstructure and performance of Quenching and Tempering mode temper bead welding and general welded joints which were made on SA508-3 steel of 60 mm thickness were compared in this article. The result shows ...The HAZ microstructure and performance of Quenching and Tempering mode temper bead welding and general welded joints which were made on SA508-3 steel of 60 mm thickness were compared in this article. The result shows that tempering sorbite which has excellent overall performance was obtained in both modes. The microstructure of Quenching and Tempering mode welded joints got more fine grain. Even though the hardness of tempering bead welded joints is higher than the general one,it still meets the standards which is lower than 350 HV. The impact absorbing energy of each district of tempering bead welded joints HAZ reached 170 J,which is equal to general one.展开更多
The transcultural communication that characterizes our times needs to be carried out in a few commonly shared languages and this requirement has sharpened the awareness about uneven language status. While defenders of...The transcultural communication that characterizes our times needs to be carried out in a few commonly shared languages and this requirement has sharpened the awareness about uneven language status. While defenders of minor languages frequently make use of such concepts as ecosystem, human rights, capital, power, imperialism, etc. To express alarm at the spread of the English language in the present world, researchers tend to forget that these concepts applied to languages are only metaphors which, taken at their face value, would conceal the non- exclusive nature of language as a communicative instrument. A question is raised as to whether we should go beyond an essentially nationalist perception of language inherited from the nineteenth century.展开更多
Since the Modbus RTU wired communication protocol of Siemens variable frequency motors is unstable and lacks a protection mechanism, there is a risk of user information leakage. Aiming at the problems of insufficient ...Since the Modbus RTU wired communication protocol of Siemens variable frequency motors is unstable and lacks a protection mechanism, there is a risk of user information leakage. Aiming at the problems of insufficient flexibility of traditional defense methods and poor defense effects, The present work proposed a new dual detection method based on MODBUS RTU, which combines the dual monitoring mechanism of “Address Resolution Protocol (ARP) request detection” and “ARP response detection”. In order to improve detection efficiency, two real-time updated linear tables are introduced, which can effectively deal with the three ARP spoofing methods of updating the ARP buffer. Based on the analysis of the hidden dangers of the Modbus RTU wired communication protocol, a wired connection between the S7-1200 PLC and the variable frequency motor was established, and a real experimental platform was constructed to demonstrate the attack. The intensity of ARP attacks has gradually increased over time. Through comparative experiments with traditional defense methods, it is proved that the algorithm enhances the protocol mechanism in principle, and is more flexible and reliable than traditional methods.展开更多
The remote terminal unit(RTU)is an important part of the power grid dispatching automation in the distribution automation(SCADA).The design of LPC2294 RTU based on ARM7TDMI(-S)chip has been discussed. The hardware and...The remote terminal unit(RTU)is an important part of the power grid dispatching automation in the distribution automation(SCADA).The design of LPC2294 RTU based on ARM7TDMI(-S)chip has been discussed. The hardware and software design of 2294 has been introduced in detail.展开更多
Many national governments have employed strategic communication in promoting their external images. Though somewhat a sub-nation since its handover to China in 1999, the Macao Special Administrative Region Government ...Many national governments have employed strategic communication in promoting their external images. Though somewhat a sub-nation since its handover to China in 1999, the Macao Special Administrative Region Government （MSARG） finds it imperative to apply strategic communication to its place branding in its regional and international communities. Taking on the former Portuguese colony for an in-depth analysis, this study describes the perception of, knowledge about, and practice on strategic communication by the MSARG communication officials. The findings, though limited, seem reconcilable with the analytical assumptions of strategic communication studies in the west.展开更多
Aiming at the actual demand for monitoring environmental information,a wireless sensing system for temperature and relative humidity(RH)monitoring based on radio frequency(RF)technology and mobile network was designed...Aiming at the actual demand for monitoring environmental information,a wireless sensing system for temperature and relative humidity(RH)monitoring based on radio frequency(RF)technology and mobile network was designed.This paper introduces the architecture of the system.The system uses AVR micro controller unit(MCU),KYL-1020U RF module and SHT71 to complete real-time temperature and humidity monitoring,and uses SIM900A module to realize remote alarming and monitoring with short message system(SMS)through global system for mobile communication(GSM).Experimental results show that the designed system has good stability of measurement and real-time performance,and it can be used in some small temperature and humidity monitoring occasions.展开更多
In this paper, we proposed a 2-channel demultiplexer based on photonic crystal ring resonator （PCRR）. For performing wavelength selection, we used two ring resonators, two different wavelengths were obtained by usin...In this paper, we proposed a 2-channel demultiplexer based on photonic crystal ring resonator （PCRR）. For performing wavelength selection, we used two ring resonators, two different wavelengths were obtained by using two resonant rings with different values for the radius of dielectric rods. All the simulations and calculations have been done using Rsoft Photonic CAD software, which employs finite difference time domain （FDTD） method. The output channels were respectively at 1590.8 and 1593.8nm, correspondingly had the quality factors of 7954 and 3984, the crosstalk values of -22 and -11 dB separately. The total footprint of our proposed structure is 681.36 μm2. Results suggest that 2-channels in the proposed structure are characterized with high transmission efficiency and low band width, resulting in a very sharp output spectrum and high quality factor values.展开更多
基金Supported by a grant from the National Natural Sciences Foundation of China(No.30801138)
文摘Objective： The aim of our study was to investigate the effects of （-）-epigallocatechin-3-gallate （EGCG, the major phytochemistry component in green tea） on the expression of connexin43 （Cx43） gene and detect the intercellular communication of the human bladder cancer cell lines BIU-87, and explore its possible mechanisms of prevention and cure for the bladder tumor. Methods： The methyl thiazolyl tetrazolium and Annexin-V/PI double-labeled flow cytometry methods were used to observe the growth inhibitory rate （IR） and apoptosis rate （AR） of BlU-87 cells treated by EGCG at different concentrations （0, 5, 10 and 20 mg/L）, respectively. The reverse transcription-polymerase chain reaction （RT-PCR） and Western Blotting analysis were employed to detect the relative expression levels of the Cx43 mRNA and its protein. The scrape-loading fluorescence dye transfer method was used to assess the gap junction intercellular communication （GJIC） under fluorescence microscope. Results： EGCG at concentrations （10 and 20 mg/L） both could significantly inhibit the proliferation and induce the apoptosis of BIU-87 cells. The IR and AR were （15.67 ± 1.15）%, （18.33 ± 1.53）% and （42.00 ± 4.34）%, （27.33 ± 3.21）%, respectively. And compared with the control groups of 0 mg/L and 5 mg/L （P 〈 0.05）, EGCG could significantly up-regulate the expression of Cx43 mRNA and its protein and enhance the function of BIU-87 cells. The effects had the significant correlation with the dose-dependent of EGCG. Conclusion： EGCG （10, 20 mg/L） could effectively up-regulate Cx43 expression and enhance the GJIC of BlU-87 cells. The results may indicate the effects of EGCG inducing bladder tumor cells apoptosis and inhibiting its growth which provides the experimental evidence for further demonstrating the mechanism of chemical prevention and cure for the bladder tumor by EGCG.
基金supported by the Key State Science and Technology Projects(Grant No.2011ZX04016-061 and No.2012ZX06004-001-001-005)
文摘The HAZ microstructure and performance of Quenching and Tempering mode temper bead welding and general welded joints which were made on SA508-3 steel of 60 mm thickness were compared in this article. The result shows that tempering sorbite which has excellent overall performance was obtained in both modes. The microstructure of Quenching and Tempering mode welded joints got more fine grain. Even though the hardness of tempering bead welded joints is higher than the general one,it still meets the standards which is lower than 350 HV. The impact absorbing energy of each district of tempering bead welded joints HAZ reached 170 J,which is equal to general one.
文摘The transcultural communication that characterizes our times needs to be carried out in a few commonly shared languages and this requirement has sharpened the awareness about uneven language status. While defenders of minor languages frequently make use of such concepts as ecosystem, human rights, capital, power, imperialism, etc. To express alarm at the spread of the English language in the present world, researchers tend to forget that these concepts applied to languages are only metaphors which, taken at their face value, would conceal the non- exclusive nature of language as a communicative instrument. A question is raised as to whether we should go beyond an essentially nationalist perception of language inherited from the nineteenth century.
文摘Since the Modbus RTU wired communication protocol of Siemens variable frequency motors is unstable and lacks a protection mechanism, there is a risk of user information leakage. Aiming at the problems of insufficient flexibility of traditional defense methods and poor defense effects, The present work proposed a new dual detection method based on MODBUS RTU, which combines the dual monitoring mechanism of “Address Resolution Protocol (ARP) request detection” and “ARP response detection”. In order to improve detection efficiency, two real-time updated linear tables are introduced, which can effectively deal with the three ARP spoofing methods of updating the ARP buffer. Based on the analysis of the hidden dangers of the Modbus RTU wired communication protocol, a wired connection between the S7-1200 PLC and the variable frequency motor was established, and a real experimental platform was constructed to demonstrate the attack. The intensity of ARP attacks has gradually increased over time. Through comparative experiments with traditional defense methods, it is proved that the algorithm enhances the protocol mechanism in principle, and is more flexible and reliable than traditional methods.
文摘The remote terminal unit(RTU)is an important part of the power grid dispatching automation in the distribution automation(SCADA).The design of LPC2294 RTU based on ARM7TDMI(-S)chip has been discussed. The hardware and software design of 2294 has been introduced in detail.
文摘Many national governments have employed strategic communication in promoting their external images. Though somewhat a sub-nation since its handover to China in 1999, the Macao Special Administrative Region Government （MSARG） finds it imperative to apply strategic communication to its place branding in its regional and international communities. Taking on the former Portuguese colony for an in-depth analysis, this study describes the perception of, knowledge about, and practice on strategic communication by the MSARG communication officials. The findings, though limited, seem reconcilable with the analytical assumptions of strategic communication studies in the west.
文摘Aiming at the actual demand for monitoring environmental information,a wireless sensing system for temperature and relative humidity(RH)monitoring based on radio frequency(RF)technology and mobile network was designed.This paper introduces the architecture of the system.The system uses AVR micro controller unit(MCU),KYL-1020U RF module and SHT71 to complete real-time temperature and humidity monitoring,and uses SIM900A module to realize remote alarming and monitoring with short message system(SMS)through global system for mobile communication(GSM).Experimental results show that the designed system has good stability of measurement and real-time performance,and it can be used in some small temperature and humidity monitoring occasions.
文摘In this paper, we proposed a 2-channel demultiplexer based on photonic crystal ring resonator （PCRR）. For performing wavelength selection, we used two ring resonators, two different wavelengths were obtained by using two resonant rings with different values for the radius of dielectric rods. All the simulations and calculations have been done using Rsoft Photonic CAD software, which employs finite difference time domain （FDTD） method. The output channels were respectively at 1590.8 and 1593.8nm, correspondingly had the quality factors of 7954 and 3984, the crosstalk values of -22 and -11 dB separately. The total footprint of our proposed structure is 681.36 μm2. Results suggest that 2-channels in the proposed structure are characterized with high transmission efficiency and low band width, resulting in a very sharp output spectrum and high quality factor values.
