The interaction of Ru(phen) 2(dppz) 2+ (phen=1,10 phenanthroline,dppz=dipyrido[3,2 a:2′,3′ c]phenazine) and the calfthymus DNA were studied with fluorescence and ultraviolet visible absorption spectroscopy....The interaction of Ru(phen) 2(dppz) 2+ (phen=1,10 phenanthroline,dppz=dipyrido[3,2 a:2′,3′ c]phenazine) and the calfthymus DNA were studied with fluorescence and ultraviolet visible absorption spectroscopy. The ultraviolet visible absorption spectrum of Ru(phen) 2(dppz) 2+ , calfthymus DNA, and their interaction demonstrated that Ru(phen) 2(dppz) 2+ intercalated into the double helix of DNA via the ligand dppz. Fe(CN) 6 4- and NaCl can not quench the fluorescence of Ru(phen) 2(dppz) 2+ DNA, which was in agreement with the intercalation mode.展开更多
The binding mode of Eu(phen)_3^(3+) with DNA is studied by fluorescence andvoltammetric methods. From the Scatchard graph and the fluorescence quenching phenomenon, it isconcluded that the mode of interaction between ...The binding mode of Eu(phen)_3^(3+) with DNA is studied by fluorescence andvoltammetric methods. From the Scatchard graph and the fluorescence quenching phenomenon, it isconcluded that the mode of interaction between Eu(phen)_3^(3+) and DNA is intercalation bind byinserting the phen pi-pi conjugate surface between the base pairs of the DNA duplex. Voltammetry wasused to confirm the results obtained from the fluorescence method, the result from both methodsagrees with each other. The binding constant (K) and the binding site size (n_s) were calculatedfrom voltammetric data such as the shifts in potential and limiting currents in the process ofadding DNA, according to the positive shifts of peak potential. It was considered that the complexof the +2 ion interacted more favorably with the nucleotide bases than that of the +3 ion byhydrophobic interaction.展开更多
文摘The interaction of Ru(phen) 2(dppz) 2+ (phen=1,10 phenanthroline,dppz=dipyrido[3,2 a:2′,3′ c]phenazine) and the calfthymus DNA were studied with fluorescence and ultraviolet visible absorption spectroscopy. The ultraviolet visible absorption spectrum of Ru(phen) 2(dppz) 2+ , calfthymus DNA, and their interaction demonstrated that Ru(phen) 2(dppz) 2+ intercalated into the double helix of DNA via the ligand dppz. Fe(CN) 6 4- and NaCl can not quench the fluorescence of Ru(phen) 2(dppz) 2+ DNA, which was in agreement with the intercalation mode.
基金This project is financially supported by the Natural Foundation of Gansu Province (GZ 97-0025) NWNUKJCXGC-01(No. 001)
文摘The binding mode of Eu(phen)_3^(3+) with DNA is studied by fluorescence andvoltammetric methods. From the Scatchard graph and the fluorescence quenching phenomenon, it isconcluded that the mode of interaction between Eu(phen)_3^(3+) and DNA is intercalation bind byinserting the phen pi-pi conjugate surface between the base pairs of the DNA duplex. Voltammetry wasused to confirm the results obtained from the fluorescence method, the result from both methodsagrees with each other. The binding constant (K) and the binding site size (n_s) were calculatedfrom voltammetric data such as the shifts in potential and limiting currents in the process ofadding DNA, according to the positive shifts of peak potential. It was considered that the complexof the +2 ion interacted more favorably with the nucleotide bases than that of the +3 ion byhydrophobic interaction.
