Diabetic retinopathy(DR)is one of the major causes of visual impairment and irreversible blindness in developed regions.Aside from abnormal angiogenesis,inflammation is the most specific and might be the initiating fa...Diabetic retinopathy(DR)is one of the major causes of visual impairment and irreversible blindness in developed regions.Aside from abnormal angiogenesis,inflammation is the most specific and might be the initiating factor of DR.As a key participant in inflammation,interferon-gamma(IFN-γ)can be detected in different parts of the eye and is responsible for the breakdown of the blood-retina barrier and activation of inflammatory cells and other cytokines,which accelerate neovascularization and neuroglial degeneration.In addition,IFN-γis involved in other vascular complications of diabetes mellitus and angiogenesis-dependent diseases,such as diabetic nephropathy,cerebral microbleeds,and age-related macular degeneration.Traditional treatments,such as anti-vascular endothelial growth factor agents,vitrectomy,and laser photocoagulation therapy,are more effective for angiogenesis and not tolerable for every patient.Many ongoing clinical trials are exploring effective drugs that target inflammation.For instance,IFN-αacts against viruses and angiogenesis and is commonly used to treat malignant tumors.Moreover,IFN-αhas been shown to contribute to alleviating the progression of DR and other ocular diseases.In this review,we emphasize the roles that IFNs play in the pathogenesis of DR and discuss potential clinical applications of IFNs in DR,such as diagnosis,prognosis,and therapeutic treatment.展开更多
Macrophages(Mφs) are not only a kind of immune effector cells but also a kind of antigenpresenting cells(APC). In order to improve their antitumor effect, we transfected interferongamma(IFNγ) gene into Mφs by recom...Macrophages(Mφs) are not only a kind of immune effector cells but also a kind of antigenpresenting cells(APC). In order to improve their antitumor effect, we transfected interferongamma(IFNγ) gene into Mφs by recombinant adenovirus because IFNγis a kind of potent macrophageactivating factor(MAF). High level of IFNγ could be detected in the supernatants of Mφs after IFNγ gene transfection and IFNγ secretion peaked at 20 hour. The cytotoxicity of IFNγgenetransfected Mφs increased significantly. The secretion of TNF, IL1, nitric oxide(NO) also increased to some extent. The results demonstrated that recombinant adenovirusmediated IFNγ gene transfection could improve the effector functions of Mφs efficiently.展开更多
基金Supported by National Natural Science Foundation of China,No.81800855 and No.82070967Natural Science Foundation of Hunan Province,No.2018JJ3765.
文摘Diabetic retinopathy(DR)is one of the major causes of visual impairment and irreversible blindness in developed regions.Aside from abnormal angiogenesis,inflammation is the most specific and might be the initiating factor of DR.As a key participant in inflammation,interferon-gamma(IFN-γ)can be detected in different parts of the eye and is responsible for the breakdown of the blood-retina barrier and activation of inflammatory cells and other cytokines,which accelerate neovascularization and neuroglial degeneration.In addition,IFN-γis involved in other vascular complications of diabetes mellitus and angiogenesis-dependent diseases,such as diabetic nephropathy,cerebral microbleeds,and age-related macular degeneration.Traditional treatments,such as anti-vascular endothelial growth factor agents,vitrectomy,and laser photocoagulation therapy,are more effective for angiogenesis and not tolerable for every patient.Many ongoing clinical trials are exploring effective drugs that target inflammation.For instance,IFN-αacts against viruses and angiogenesis and is commonly used to treat malignant tumors.Moreover,IFN-αhas been shown to contribute to alleviating the progression of DR and other ocular diseases.In this review,we emphasize the roles that IFNs play in the pathogenesis of DR and discuss potential clinical applications of IFNs in DR,such as diagnosis,prognosis,and therapeutic treatment.
文摘Macrophages(Mφs) are not only a kind of immune effector cells but also a kind of antigenpresenting cells(APC). In order to improve their antitumor effect, we transfected interferongamma(IFNγ) gene into Mφs by recombinant adenovirus because IFNγis a kind of potent macrophageactivating factor(MAF). High level of IFNγ could be detected in the supernatants of Mφs after IFNγ gene transfection and IFNγ secretion peaked at 20 hour. The cytotoxicity of IFNγgenetransfected Mφs increased significantly. The secretion of TNF, IL1, nitric oxide(NO) also increased to some extent. The results demonstrated that recombinant adenovirusmediated IFNγ gene transfection could improve the effector functions of Mφs efficiently.
文摘目的:研究人重组γ干扰素(Hu-rIFN-7)对小鼠的急性毒性及对大鼠、狗的长期毒性.方法:小鼠肌注或静注Hu-rIFN-γ 4.4×10~9IUm^(-2)观察一周.长期毒性研究中,肌注给予临床推荐剂量1×10~6IU m^(-2)的10,50及100倍(大鼠)及5和50倍(狗),观察血液学、血液生化、尿分析、心电图和组织器官的病理变化.结果:Hu-rIFN-γ对小鼠im或iv的最大耐受剂量为4.4×109 IU m^(-2).长期毒性试验中未发现药物相关的毒性.结论:人重组γ干扰素对大鼠及狗不产生毒性反应.
