Doxorubicin（DOX） loaded poly（lactic-co-glycolic acid）（PLGA） microparticles with internal pores（MP-D） were developed for long-acting release in pulmonary inhalation treatment. The PLGA microparticles exhibited ...Doxorubicin（DOX） loaded poly（lactic-co-glycolic acid）（PLGA） microparticles with internal pores（MP-D） were developed for long-acting release in pulmonary inhalation treatment. The PLGA microparticles exhibited favorable aerodynamic properties for pulmonary delivery. In vitro drug release profile suggested that MP-D have the advantage of long-term maintenance of drug concentrations. MTT assay demonstrated the in vitro anti-tumor efficiency of the DOX loaded PLGA microparticles. Furthermore, melanoma lung metastasis model was established to determine the in vivo antitumor efficiency. The mice treated with MP-D showed significantly fewer lesions than the untreated ones. The survival analysis indicated that MP-D prolonged the survival time of tumor-bearing mice. These results suggested that DOX loaded PLGA microparticles with internal pores have the potential to be used as long-acting release carriers in clinical lung cancer treatment.展开更多
Variation of wind speed and the physico-chemical parameters,such as dissolved phosphate,ferrous and manganese in lake water were observed on site.Together with the chemistry analysis and simulated experiment in lab,th...Variation of wind speed and the physico-chemical parameters,such as dissolved phosphate,ferrous and manganese in lake water were observed on site.Together with the chemistry analysis and simulated experiment in lab,the change of phosphate concentration in lake water was analyzed.The variation of ferrous/phosphate ratio explained that along with the enhancement of wind-wave effect and the oxidation ability of lake water,the effects of co-precipitation and removal of dissolved phosphate and iron in the lake water were reinforced.The ferrous/phosphate ratio in pore water was less than 2.0,demonstrating that the dissolved phosphate can be released into the overlying water.But,in the lake water,the stability of phosphate was controlled by the water dynamics.The phosphate release experiment showed that molecular release was only part of the whole and the direct discharge of phosphate in the pore water was also a part.The mineralization and biological process of suspended particulates in the water may be another important reason for the whole phosphate loadings.展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.51222307,51303173,51390480,21474104 and 51403205)the Ministry of Science and Technology of China(International cooperation and communication program 2011DFR51090)Jilin Province Science and Technology Development Program(Nos.20120306,20130521011JH)
文摘Doxorubicin（DOX） loaded poly（lactic-co-glycolic acid）（PLGA） microparticles with internal pores（MP-D） were developed for long-acting release in pulmonary inhalation treatment. The PLGA microparticles exhibited favorable aerodynamic properties for pulmonary delivery. In vitro drug release profile suggested that MP-D have the advantage of long-term maintenance of drug concentrations. MTT assay demonstrated the in vitro anti-tumor efficiency of the DOX loaded PLGA microparticles. Furthermore, melanoma lung metastasis model was established to determine the in vivo antitumor efficiency. The mice treated with MP-D showed significantly fewer lesions than the untreated ones. The survival analysis indicated that MP-D prolonged the survival time of tumor-bearing mice. These results suggested that DOX loaded PLGA microparticles with internal pores have the potential to be used as long-acting release carriers in clinical lung cancer treatment.
基金supported by the Knowledge Innovation Project of the Chinese Academy of Sciences(Grant No.KZCX1-SW-12)the National Natural Science Foundation of China(Grant No.40501064)the State Key Project of Science and Technology in"Tenth Five-Year Plan"of China,"863"(Grant No.2002AA601013).
文摘Variation of wind speed and the physico-chemical parameters,such as dissolved phosphate,ferrous and manganese in lake water were observed on site.Together with the chemistry analysis and simulated experiment in lab,the change of phosphate concentration in lake water was analyzed.The variation of ferrous/phosphate ratio explained that along with the enhancement of wind-wave effect and the oxidation ability of lake water,the effects of co-precipitation and removal of dissolved phosphate and iron in the lake water were reinforced.The ferrous/phosphate ratio in pore water was less than 2.0,demonstrating that the dissolved phosphate can be released into the overlying water.But,in the lake water,the stability of phosphate was controlled by the water dynamics.The phosphate release experiment showed that molecular release was only part of the whole and the direct discharge of phosphate in the pore water was also a part.The mineralization and biological process of suspended particulates in the water may be another important reason for the whole phosphate loadings.
