本研究通过对拟澳洲赤眼蜂 Trichogramma confusum、甘蓝夜蛾赤眼蜂 T. brassicae、广赤眼蜂 T.evanescens、食胚赤眼蜂 T. embryophagum,及松毛虫赤眼蜂 T. dendrolimi的 6个地理种群的 r DNA- ITS2进行克隆测序 ,又运用软件 DNAStar的...本研究通过对拟澳洲赤眼蜂 Trichogramma confusum、甘蓝夜蛾赤眼蜂 T. brassicae、广赤眼蜂 T.evanescens、食胚赤眼蜂 T. embryophagum,及松毛虫赤眼蜂 T. dendrolimi的 6个地理种群的 r DNA- ITS2进行克隆测序 ,又运用软件 DNAStar的 Meg Align程序对不同赤眼蜂属间、赤眼蜂属种间、同种不同地理种群之间以及同一赤眼蜂个体不同拷贝之间的 ITS2序列的遗传分歧及相似性进行了分析。结果表明 :赤眼蜂属与外群 ITS2序列的遗传相似性很低、赤眼蜂属内不同种之间 ITS2序列保守性适中、种内或不同地理种群之间以及同一个体不同 ITS2拷贝间非常保守。说明展开更多
Research Aims: Obesity and type 2 diabetes are known to be associated with increased risk of various types of cancer. However, the molecular biological mechanism of how the risk of cancer is increased in obesity or ty...Research Aims: Obesity and type 2 diabetes are known to be associated with increased risk of various types of cancer. However, the molecular biological mechanism of how the risk of cancer is increased in obesity or type 2 diabetes is not known. The aim this research is to investigate if the decreased expression of p27Kip1, a cell cycle repressor protein, plays a central role in this mechanism. Research Methods, Previous Studies and Theoretical Backgrounds: It is well known that the expression of p27Kip1 is increased by numerous nutritional or chemopreventive anti-cancer agents. But it has never been known that the expression of p27Kip1 could be decreased, rather than increased, and the risk of cancer could be increased, rather than decreased. This problem was solved recently and this new analytical method was used in this study. Results: 1) The expression of p27Kip1 was indeed significantly decreased in human obese type 2 diabetic individuals relative to the lean normal controls. 2) The expression of p27Kip1 was also significantly decreased in genetically obese rodents relative to the lean normal controls. Additionally, in obese rodents, the concentrations of glucose or insulin were significantly increased relative to the lean normal controls. 3) Using human cells cultured in vitro it was found that the increased concentrations of glucose or insulin decrease the expression of p27Kip1. Conclusions: These results suggest that higher concentrations of glucose or insulin increase the risk of various types of cancer in obesity or type 2 diabetes by decreasing the expression of p27Kip1.展开更多
文摘本研究通过对拟澳洲赤眼蜂 Trichogramma confusum、甘蓝夜蛾赤眼蜂 T. brassicae、广赤眼蜂 T.evanescens、食胚赤眼蜂 T. embryophagum,及松毛虫赤眼蜂 T. dendrolimi的 6个地理种群的 r DNA- ITS2进行克隆测序 ,又运用软件 DNAStar的 Meg Align程序对不同赤眼蜂属间、赤眼蜂属种间、同种不同地理种群之间以及同一赤眼蜂个体不同拷贝之间的 ITS2序列的遗传分歧及相似性进行了分析。结果表明 :赤眼蜂属与外群 ITS2序列的遗传相似性很低、赤眼蜂属内不同种之间 ITS2序列保守性适中、种内或不同地理种群之间以及同一个体不同 ITS2拷贝间非常保守。说明
文摘Research Aims: Obesity and type 2 diabetes are known to be associated with increased risk of various types of cancer. However, the molecular biological mechanism of how the risk of cancer is increased in obesity or type 2 diabetes is not known. The aim this research is to investigate if the decreased expression of p27Kip1, a cell cycle repressor protein, plays a central role in this mechanism. Research Methods, Previous Studies and Theoretical Backgrounds: It is well known that the expression of p27Kip1 is increased by numerous nutritional or chemopreventive anti-cancer agents. But it has never been known that the expression of p27Kip1 could be decreased, rather than increased, and the risk of cancer could be increased, rather than decreased. This problem was solved recently and this new analytical method was used in this study. Results: 1) The expression of p27Kip1 was indeed significantly decreased in human obese type 2 diabetic individuals relative to the lean normal controls. 2) The expression of p27Kip1 was also significantly decreased in genetically obese rodents relative to the lean normal controls. Additionally, in obese rodents, the concentrations of glucose or insulin were significantly increased relative to the lean normal controls. 3) Using human cells cultured in vitro it was found that the increased concentrations of glucose or insulin decrease the expression of p27Kip1. Conclusions: These results suggest that higher concentrations of glucose or insulin increase the risk of various types of cancer in obesity or type 2 diabetes by decreasing the expression of p27Kip1.