[ Objective] This study aimed to investigate the regulatory effects of xylooligesaccharides on intestinal microbial flora proliferation and defecation of BALB/c mice. [Method] In the intestinal microbial flora prolife...[ Objective] This study aimed to investigate the regulatory effects of xylooligesaccharides on intestinal microbial flora proliferation and defecation of BALB/c mice. [Method] In the intestinal microbial flora proliferation regulation experiment, microflom quantities of Bifidobacterium, Lactobacillus, Enterobacter and Enterococci in excrements of BALB/c mice were determined after continuous administration of high-dose[ 1.0 g/（ kg ~ bw ） ] and low-dose[ 0.5 g/（ kg ~ bw ） ] xylooligosaccharides for 14 d. In the defecation regulation experiment, constipation model of BALB/c mice was established with compound diphenexylate after con- tinuous administration of high-dose and low-dose xylooligosaccharides for 21 d, to determine the first defecation time of black excrement, weight of black excrements within 6 h and rate of intestinal propulsion of constipation mice. [ Result] Compared with the control group, after continuous administration of xyloolignsaccharides for 14 d, microflora quantifies of Lactobac//lus and B/fidobacter/um in excrements of BALB/c mice in low-dose and high-dose groups both increased significantly （ P 〈 0.05 ）. After continuous administration of xylooligosaccharidos, microflora quantities of Enterococci and Lactobacillus in control group were significantly raised （P 〈 O. 05 and P 〈 0.01, respectively） compared with those before administration; mieroflora quantities of Enterococci in high-dose and low-dose groups were signif- icantly reduced （P 〈0.05）, while microflora quantities of/zwtobac//lus and B/fidobacter/um and the B/E value increased significantly （P 〈0. 05, P 〈0.01 and P 〈 0.01, respectively）. After continuous administration of xylooligosaccharides for 21 d, compared with the model group, the first defecation time of black excre- ment in high-dose and low-dose groups was significantly shortened （ P 〈 O. 05 ）, the total weight of black.excrements within 6 h increased significantly （ P 〈 0.05 ） and the rate of intestinal propulsion was significantly enhanced （ P 〈0.01 ）. [ Conclusion ] Low-dose （0.5 g/Kg. bw） and high-dose （ 1 g/Kg. bw） xylooligosac- charides can improve the proliferation of intestinal microbial flora and prevent mice from constipation.展开更多
基金Supported by Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PHR201008293)
文摘[ Objective] This study aimed to investigate the regulatory effects of xylooligesaccharides on intestinal microbial flora proliferation and defecation of BALB/c mice. [Method] In the intestinal microbial flora proliferation regulation experiment, microflom quantities of Bifidobacterium, Lactobacillus, Enterobacter and Enterococci in excrements of BALB/c mice were determined after continuous administration of high-dose[ 1.0 g/（ kg ~ bw ） ] and low-dose[ 0.5 g/（ kg ~ bw ） ] xylooligosaccharides for 14 d. In the defecation regulation experiment, constipation model of BALB/c mice was established with compound diphenexylate after con- tinuous administration of high-dose and low-dose xylooligosaccharides for 21 d, to determine the first defecation time of black excrement, weight of black excrements within 6 h and rate of intestinal propulsion of constipation mice. [ Result] Compared with the control group, after continuous administration of xyloolignsaccharides for 14 d, microflora quantifies of Lactobac//lus and B/fidobacter/um in excrements of BALB/c mice in low-dose and high-dose groups both increased significantly （ P 〈 0.05 ）. After continuous administration of xylooligosaccharidos, microflora quantities of Enterococci and Lactobacillus in control group were significantly raised （P 〈 O. 05 and P 〈 0.01, respectively） compared with those before administration; mieroflora quantities of Enterococci in high-dose and low-dose groups were signif- icantly reduced （P 〈0.05）, while microflora quantities of/zwtobac//lus and B/fidobacter/um and the B/E value increased significantly （P 〈0. 05, P 〈0.01 and P 〈 0.01, respectively）. After continuous administration of xylooligosaccharides for 21 d, compared with the model group, the first defecation time of black excre- ment in high-dose and low-dose groups was significantly shortened （ P 〈 O. 05 ）, the total weight of black.excrements within 6 h increased significantly （ P 〈 0.05 ） and the rate of intestinal propulsion was significantly enhanced （ P 〈0.01 ）. [ Conclusion ] Low-dose （0.5 g/Kg. bw） and high-dose （ 1 g/Kg. bw） xylooligosac- charides can improve the proliferation of intestinal microbial flora and prevent mice from constipation.
