To investigate the relationship between intracellular free Ca~ 2+ concentration (Ca~ 2+_i) and calcium-activated chloride (Cl_ca ) channels of pulmonary artery smooth muscle cells (PASMCs) in rats under acute and chro...To investigate the relationship between intracellular free Ca~ 2+ concentration (Ca~ 2+_i) and calcium-activated chloride (Cl_ca ) channels of pulmonary artery smooth muscle cells (PASMCs) in rats under acute and chronic hypoxic conditions, acute hypoxia-induced contraction was observed in rat pulmonary artery by using routine blood vascular perfusion in vitro. The fluorescence Ca~ 2+ indicator Fura-2/AM was used to observe Ca~ 2+ _i of rat PASMCs under normal and chronic hypoxic condition. The effect of Cl_ ca channels on PASMCs proliferation was assessed by MTT assay. The Cl_ ca channel blockers niflumic acid (NFA) and indaryloxyacetic acid (IAA-94) exerted inhibitory effects on acute hypoxia-evoked contractions in the pulmonary artery. Under chronic hypoxic condition, Ca~ 2+ _i was increased. Under normoxic condition, Ca~ 2+ _i was (123.63±18.98) nmol/L, and in hypoxic condition, Ca~ 2+ _i was (281.75±16.48) nmol/L (P<0.01). Under normoxic condition, Ca~ 2+ _i showed no significant change and no effect on Cl_ ca channels was observed (P>0.05). Chronic hypoxia increased Ca~ 2+ _i which opened Cl_ ca channels. The NFA and IAA-94 blocked the channels and decreased Ca~ 2+ _i from (281.75±16.48) nmol/L to (117.66±15.36) nmol/L (P<0.01). MTT assay showed that under chronic hypoxic condition NFA and IAA-94 decreased the value of absorbency (A value) from 0.459±0.058 to 0.224±0.025 (P<0.01). Hypoxia increased Ca~ 2+ _i which opened Cl_ ca channels and had a positive-feedback in Ca~ 2+ _i. This may play an important role in hypoxic pulmonary hypertension. Under chronic hypoxic condition, Cl_ca channel may play a part in the regulation of proliferation of PASMCs.展开更多
To define effects of Shouerzhi capsule on intracellular free Ca 2+ and c fos genetic expression of senile dementia model rat’s hippocampus neuron cells. Methods:By means of immunochemistry to observe c fos genetic ex...To define effects of Shouerzhi capsule on intracellular free Ca 2+ and c fos genetic expression of senile dementia model rat’s hippocampus neuron cells. Methods:By means of immunochemistry to observe c fos genetic expression of senile dementia model and through Fura 2 fluorescent technology to measure free Ca 2+ of hippocampus neuron cells.Results: Shouerzhi capsule can reduce not only c fos genetic expression but also the content of intracellular free Ca 2+ of the senile dementia model rat’s hippocampus neuron cells.Conclusion: Shouerzhi capsule can regulate apoptosis of hippocampus neuron cells through reducing c fos genetic expression and intracellular free Ca 2+ content and can prevent and cure senile dementia.展开更多
文摘To investigate the relationship between intracellular free Ca~ 2+ concentration (Ca~ 2+_i) and calcium-activated chloride (Cl_ca ) channels of pulmonary artery smooth muscle cells (PASMCs) in rats under acute and chronic hypoxic conditions, acute hypoxia-induced contraction was observed in rat pulmonary artery by using routine blood vascular perfusion in vitro. The fluorescence Ca~ 2+ indicator Fura-2/AM was used to observe Ca~ 2+ _i of rat PASMCs under normal and chronic hypoxic condition. The effect of Cl_ ca channels on PASMCs proliferation was assessed by MTT assay. The Cl_ ca channel blockers niflumic acid (NFA) and indaryloxyacetic acid (IAA-94) exerted inhibitory effects on acute hypoxia-evoked contractions in the pulmonary artery. Under chronic hypoxic condition, Ca~ 2+ _i was increased. Under normoxic condition, Ca~ 2+ _i was (123.63±18.98) nmol/L, and in hypoxic condition, Ca~ 2+ _i was (281.75±16.48) nmol/L (P<0.01). Under normoxic condition, Ca~ 2+ _i showed no significant change and no effect on Cl_ ca channels was observed (P>0.05). Chronic hypoxia increased Ca~ 2+ _i which opened Cl_ ca channels. The NFA and IAA-94 blocked the channels and decreased Ca~ 2+ _i from (281.75±16.48) nmol/L to (117.66±15.36) nmol/L (P<0.01). MTT assay showed that under chronic hypoxic condition NFA and IAA-94 decreased the value of absorbency (A value) from 0.459±0.058 to 0.224±0.025 (P<0.01). Hypoxia increased Ca~ 2+ _i which opened Cl_ ca channels and had a positive-feedback in Ca~ 2+ _i. This may play an important role in hypoxic pulmonary hypertension. Under chronic hypoxic condition, Cl_ca channel may play a part in the regulation of proliferation of PASMCs.
文摘To define effects of Shouerzhi capsule on intracellular free Ca 2+ and c fos genetic expression of senile dementia model rat’s hippocampus neuron cells. Methods:By means of immunochemistry to observe c fos genetic expression of senile dementia model and through Fura 2 fluorescent technology to measure free Ca 2+ of hippocampus neuron cells.Results: Shouerzhi capsule can reduce not only c fos genetic expression but also the content of intracellular free Ca 2+ of the senile dementia model rat’s hippocampus neuron cells.Conclusion: Shouerzhi capsule can regulate apoptosis of hippocampus neuron cells through reducing c fos genetic expression and intracellular free Ca 2+ content and can prevent and cure senile dementia.