To study the differences in homing potential between bone marrow cells and umbilical blood cells, CD34 positive cells were obtained from bone marrow (BM) and umbilical blood (UB) by the direct cell separation with dom...To study the differences in homing potential between bone marrow cells and umbilical blood cells, CD34 positive cells were obtained from bone marrow (BM) and umbilical blood (UB) by the direct cell separation with domestic immunomagnetic beads. The expression of the two adhesion molecules CD11a/CD18 and CD44 were examined. After separation, CD34 positive cells accounted for 51 %-82 % of the harvested cells and dye-resistance rate was 82 %-88 %. The expression of CD11a/CD18 and CD44 on the surfaces of UB cells was 49. 6 % 1 10. 2 % and 37. 7 % ± 10. 3 % respectively. On BM cells they were 50. 2 % ± 6. 2 % and 34 % ± 13. 3 % respectively. There were no significant differences in the expression of these two molecules. It was concluded .that the cell separation method with domestic immunomagnetic beads was effective and the stem cells from UB could serve as an alternative source for transplantation.展开更多
We propose a wavelet method to analyze the stochastic-elastic problem of specific adhesion between two elastic solids via ligand-receptor bond clusters, which is governed by a nonlinear integro-differential equation w...We propose a wavelet method to analyze the stochastic-elastic problem of specific adhesion between two elastic solids via ligand-receptor bond clusters, which is governed by a nonlinear integro-differential equation with a sin- gular Cauchy kernel to describe the mean-field coupling between deformation of elastic materials and stochastic behavior of the molecular bonds. To solve this problem, Galerkin method based on a wavelet approximation scheme is adopted, and special treatment which transforms the singular Cauchy kernel into a smooth one has been proposed to avoid the cumbersome calculation of singular integrals. Numerical results demonstrate that the method is fully capable of solving the specific adhesion problems with complex nonlinear and singular equations. Based on the proposed method, investigations are performed to reveal the relation between steady-state pulling force and mean surface separation under different stress concentration indexes, which is crucial for assembling the overall constitutive relations for multicellular tumor spheroids and polymer-matrix microcomposites.展开更多
文摘To study the differences in homing potential between bone marrow cells and umbilical blood cells, CD34 positive cells were obtained from bone marrow (BM) and umbilical blood (UB) by the direct cell separation with domestic immunomagnetic beads. The expression of the two adhesion molecules CD11a/CD18 and CD44 were examined. After separation, CD34 positive cells accounted for 51 %-82 % of the harvested cells and dye-resistance rate was 82 %-88 %. The expression of CD11a/CD18 and CD44 on the surfaces of UB cells was 49. 6 % 1 10. 2 % and 37. 7 % ± 10. 3 % respectively. On BM cells they were 50. 2 % ± 6. 2 % and 34 % ± 13. 3 % respectively. There were no significant differences in the expression of these two molecules. It was concluded .that the cell separation method with domestic immunomagnetic beads was effective and the stem cells from UB could serve as an alternative source for transplantation.
基金supported by the National Natural Science Foundation of China(11032006 and 11121202)National Key Project of Magneto-Constrained Fusion Energy Development Program(2013GB110002)the Fundamental Research Funds for the Central Universities(lzujbky-2013-1)
文摘We propose a wavelet method to analyze the stochastic-elastic problem of specific adhesion between two elastic solids via ligand-receptor bond clusters, which is governed by a nonlinear integro-differential equation with a sin- gular Cauchy kernel to describe the mean-field coupling between deformation of elastic materials and stochastic behavior of the molecular bonds. To solve this problem, Galerkin method based on a wavelet approximation scheme is adopted, and special treatment which transforms the singular Cauchy kernel into a smooth one has been proposed to avoid the cumbersome calculation of singular integrals. Numerical results demonstrate that the method is fully capable of solving the specific adhesion problems with complex nonlinear and singular equations. Based on the proposed method, investigations are performed to reveal the relation between steady-state pulling force and mean surface separation under different stress concentration indexes, which is crucial for assembling the overall constitutive relations for multicellular tumor spheroids and polymer-matrix microcomposites.
