Intron Retention Fine-Tunes the Resistance of the Rice Mutant pls4 to Rice Sheath Blight(Rhizotonia solani AG I.1a)

OsPLS4 encodes aβ-ketoacyl carrier protein reductase(KAR).The role of OsPLS4 in rice sheath blight(Rhizoctonia solani)remains unclear.Our preliminary studies showed that premature leaf senescence mutants(pls4)were highly susceptive to sheath blight in the early stage of rice development.To explore the role of this gene in the development of rice sheath blight,the transcriptome profiles of the rice pls4 mutant and wild type were compared by RNA-seq.The results revealed 2,569 differentially expressed genes(DEGs).The down-regulated genes were significantly enriched in the defense response-related biological processes.These down-regulated genes included the chitinase genes and WRKY genes,which were significantly changed in pls4 mutants.Furthermore,467 genes induced significant alternative splicing(AS)events.Among them,intron retention(IR)affected gene expression levels and functions of the vitamin B6(VB6)metabolism pathway related to sheath blight.This result suggests that IR plays an important role in the sheath blight resistance of mutant pls4.Together,these results indicate that pls4 could be involved in the biological process of sheath blight via DEGs and the fine-tuning of IR.The present study provides a molecular basis for further investigation of the resistance of rice to sheath blight.

Assessment of Retention Ponds and Its Impacts on Health of Residents in Mogadishu, Somalia: Mixed Methods

The purpose of this study was to evaluate the effects of retention ponds on the environment and population health by analyzing water samples from various ponds in Mogadishu, to determine the prevalence of waterborne illnesses that occur during the rainy season in Mogadishu, and to find out what experts thought about the effects of retention ponds on the environment as well as population health in Mogadishu. Methods: Mixed designs were used in the study. The first design is an exploratory study where samples are taken from different retention ponds in Mogadishu. The second design involves gathering secondary data from the online FSNAU Dashboard regarding the incidence of rainfall and waterborne illnesses including malaria and cholera. Additionally, a cross-sectional survey of expert opinions using questionnaires was the third design. The 10 water samples were taken from retention ponds in Mogadishu as part of the sample size. Data on the fourth month was also gathered using the FNSAU dashboard, and seventy sample sizes were used for the expert self-administered questionnaire for the third design. Excel was used for data analysis in the initial design. While BMI SPSS versions 22 were used to analyze the data from the Self-administered Questionnaire, additional methods were utilized to compute descriptive statistics, such as mean and standard deviation, and to analyze demographic data in a frequency table. Findings: The results show that three samples had unsatisfactory scores (Grade D): Yaqshid (Warshadda Bastada) had a WQI of 80.85, Boondheer (Bondher Pond) had a WQI of 80.64, and Wartanabad (Xamar Jadiid Pond) had a WQI of 80.89. The remaining samples were all rated as fair (grade), which indicates that they ranged from 50 to 75. The months with the largest rainfall already occurred in December, November, and October, when the prevalence of diseases during the rainy season was highest for cholera cases. Although October and December saw a significant number of malaria cases, November did not. Retention ponds’ overall effects on residential environments were evaluated, and the results showed that the standard deviation was 0.802 and the cumulative average mean scores were 4.41 overall. This indicates that the respondents were in agreement that retention ponds in Mogadishu, Somalia, had an effect on residential areas. Recommendation: The study suggested that in order to identify retention pond contamination and create treatment units for its management, the Ministry of Health forms a district-level public health committee. All districts must have a sewer system installed by the local government, and retention ponds must be made easier in order to move waste outside of the city.

The landscape and clinical relevance of intronic polyadenylation in human cancers

Intronic polyadenylation(IPA)is an RNA 3'end processing event which has been reported to play important roles in cancer development.However,the comprehensive landscape of IPA events across various cancer types is lacking.Here,we apply IPAFinder to identify and quantify IPA events in 10,383 samples covering all 33 cancer types from The Cancer Genome Atlas(TCGA)project.We identify a total of 21,835 IPA events,almost half of which are ubiquitously expressed.We identify 2761 unique dynamically changed IPA events across cancer types.Furthermore,we observe 8855 non-redundant clinically relevant IPA events,which could potentially be used as prognostic indicators.Our analysis also reveals that dynamic IPA usage within cancer signaling pathways may affect drug response.Finally,we develop a user-friendly data portal,IPACancer Atlas(http://www.tingni-lab.com/Pancan_IPA),to search and explore IPAs in cancer.

Fire and retention island remnants have similar deadwood carbon stock a decade after disturbances in boreal forests of Alberta

In an attempt to reconcile wood extraction and forest biodiversity in managed boreal forests,ecosystem-based forest management(EBM)has become the de facto management approach.Retention forestry represents one prominent way that EBM is implemented in many parts of the world.Retention patches commonly left after harvesting serve as analogues of fire island remnants,which are patches of unburned forests in the burned forest matrix.Although the persistence of retention patches has been questioned,few studies have attempted to quantitatively compare forest attributes in both burned and harvested forests.As part of a larger program examining multiple aspects of ecosystem function in fire and harvest island remnants,we investigated the impact of disturbance type(fire/harvest)and forest edges on C stock in snags and coarse woody debris(CWD)found in island remnants in mixedwood boreal forests of Alberta,Canada.Total C stock(in snags and CWD)was similar between the two disturbance types and edge plots had similar total deadwood C stocks to interiors.The edges of island remnants had about two-fold more snag C stock than their interiors in both disturbance types,but C stock in CWD was unaffected by edge effects and disturbance type.Our results suggest that deadwood C dynamics in island remnants in fire and harvest disturbed boreal forests were similar,thus lending support for the continued implementation of retention forestry in Alberta.

Mutations and intron polymorphisms in voltage-gated sodium channel genes of different geographic populations of Culex pipiens pallens/Culex pipiens quinquefasciatus in China

Background Culex pipiens pallens and Culex pipiens quinquefasciatus are the dominant species of Culex mosquitoes in China and important disease vectors.Long-term use of insecticides can cause mutations in the voltage-gated sodium channel(vgsc)gene of mosquitoes,but little is known about the current status and evolutionary origins of vgsc gene in different geographic populations.Therefore,this study aimed to determine the current status of vgsc genes in Cx.p.pallens and Cx.p.quinquefasciatus in China and to investigate the evolutionary inheritance of neighboring downstream introns of the vgsc gene to determine the impact of insecticides on long-term evolution.Methods Sampling was conducted from July to September 2021 in representative habitats of 22 provincial-level administrative divisions in China.Genomic DNA was extracted from 1308 mosquitoes,the IIS6 fragment of the vgsc gene on the nerve cell membrane was amplified using polymerase chain reaction,and the sequence was used to evaluate allele frequency and knockdown resistance(kdr)frequency.MEGA 11 was used to construct neighborjoining(NJ)tree.PopART was used to build a TCS network.Results There were 6 alleles and 6 genotypes at the L1014 locus,which included the wild-type alleles TTA/L and CTA/L and the mutant alleles TTT/F,TTC/F,TCT/S and TCA/S.The geographic populations with a kdr frequency less than 20.00%were mainly concentrated in the regions north of 38°N,and the geographic populations with a kdr frequency greater than 80.00%were concentrated in the regions south of 30°N.kdr frequency increased with decreasing latitude.And within the same latitude,the frequency of kdr in large cities is relatively high.Mutations were correlated with the number of introns.The mutant allele TCA/S has only one intron,the mutant allele TTT/F has three introns,and the wild-type allele TTA/L has 17 introns.Conclusions Cx.p.pallens and Cx.p.quinquefasciatus have developed resistance to insecticides in most regions of China.The neighboring downstream introns of the vgsc gene gradually decreased to one intron with the mutation of the vgsc gene.Mutations may originate from multiple mutation events rather than from a single origin,and populations lacking mutations may be genetically isolated.

Evaluating the impact of safety pictogram training on comprehension scores and knowledge retention among engineering students

Safety pictograms are essential tools for identifying workplace hazards by providing critical information about health hazard risks,fire safety,emergency evacuation,and accident prevention.Effective safety pictogram training programs are necessary to enhance workers'knowledge of these pictograms.This study evaluates the effectiveness of a safety pictogram training program on the comprehension and retention of knowledge among engineering students.A total of 262 participants were asked to predict the meaning of 22 safety pictograms regulated by International Organization for Standardization(IsO)7010 before and after a one-hour online training session.A follow-up test was administered six months later to assess long-term knowledge retention.Results showed that the average comprehension rate increased from 60.1%before training to 68.3%after training,with a retention rate of 66.0%six months after training.The study found that training positively affected comprehension of emergency and mandatory action pictograms,while lower scores were observed for warning pictograms.Statistical tests revealed a significant effect of training on comprehension levels 16 out of 22 pictograms,with an average increase in comprehension of 11.2%.Of these 16 pictograms,the comprehension level of 10 pictograms increased after training and remained at the same level six months later.However,the scores decreased slightly six months after the intervention,indicating the need for continued reinforcement or retraining.These findings have important implications for safety education and training programs,particularly in industries where safety hazards are widespread.The positive impact of training on comprehension scores highlights the ongoing need to improve safety pictogram comprehension to consistently meet standard acceptance criteria.Future training programs may need to focus on categories such as warning pictograms and fire equipment and fire action pictograms,which exhibited lower comprehension scores,to ensure better employee understanding.

Factors Promoting Retention of Nurses in Three Private Hospitals of Lahore: A Cross Sectional Descriptive Survey

Introduction: Pakistan has a large number of professional nurses but a higher rate of turnover contributes towards the shortage of nurses which is intensified by nurses’ immigration, lack of recruitment of new nurses and retirement of senior nurses. Aim: The study aimed at examining the factors that increase retention of nurses. Research Method: The study is descriptive cross sectional with a sample of 150 nurses including both the genders, from 3 private hospitals located in Pakistan. A convenient sampling technique was used. The data collection was done through close ended questionnaires based on 20 questions. Data collection was done quantitatively and then data was entered in SPSS. Correlation and regression tests were performed. Findings: The analysis revealed that 91.3% nurses are loyal to their hospitals but, 8.7%nurses can switch the hospital for different reasons. It was also found that the idea of leaving the job based on the intention of moving to abroad and public sector was higher than the retirement and other causes. Resultantly, enhancements in nurses’ work environments have the ability to decrease nurses’ job burnout and turnover and increase patients’ satisfaction. Recommendations: Hospital leaders should implement effective strategies to encourage nurses to continue their job by motivation, strengthening management skills within the hospital and improving a positive work environment by promoting teamwork, continuous learning, trust, respect and flexible arrangements which can lead towards achieving better results.However, further studies quantitatively and qualitatively can explore more factors related to dissatisfaction of nurses. Health systems policy makers should lead by developing related strategies for the retention of nurses.

Bio-Based Waterborne Poly(Vanillin-Butyl Acrylate)/MXene Coatings for Leather with Desired Warmth Retention and Antibacterial Properties

This study presents a solvent-free,facile synthesis of a bio-based green antibacterial agent and aromatic monomer methacrylated vanillin(MV)using vanillin.The resulting MV not only imparted antibacterial properties to coatings layered on leather,but could also be employed as a green alternative to petroleum-based carcinogen styrene(St).Herein,MV was copolymerized with butyl acrylate(BA)to obtain waterborne bio-based P(MV-BA)miniemulsion via miniemulsion polymerization.Subsequently,MXene nanosheets with excellent photothermal conversion performance and antibacterial properties,were introduced into the P(MV-BA)miniemulsion by ultrasonic dispersion.During the gradual solidification of P(MV-BA)/MXene nanocomposite miniemulsion on the leather surface,MXene gradually migrated to the surface of leather coatings due to the cavitation effect of ultrasonication and amphiphilicity of MXene,which prompted its full exposure to light and bacteria,exerting the maximum photothermal conversion efficiency and significant antibacterial efficacy.In particular,when the dosage of MXene nanosheets was 1.4 wt%,the surface temperature of P(MV-BA)/MXene nanocomposite miniemulsioncoated leather(PML)increased by about 15℃ in an outdoor environment during winter,and the antibacterial rate against Escherichia coli and Staphylococcus aureus was nearly 100%under the simulated sunlight treatment for 30 min.Moreover,the introduction of MXene nanosheets increased the air permeability,water vapor permeability,and thermal stability of these coatings.This study provides a new insight into the preparation of novel,green,and waterborne bio-based nanocomposite coatings for leather,with desired warmth retention and antibacterial properties.It can not only realize zerocarbon heating based on sunlight in winter,reducing the use of fossil fuels and greenhouse gas emissions,but also improve ability to fight off invasion by harmful bacteria,viruses,and other microorganisms.

Establishment of predictive models and determinants of preoperative gastric retention in endoscopic retrograde cholangiopancreatography

BACKGROUND Study on influencing factors of gastric retention before endoscopic retrograde cholangiopancreatography(ERCP)background:With the wide application of ERCP,the risk of preoperative gastric retention affects the smooth progress of the operation.The study found that female,biliary and pancreatic malignant tumor,digestive tract obstruction and other factors are closely related to gastric retention,so the establishment of predictive model is very important to reduce the risk of operation.METHODS A retrospective analysis was conducted on 190 patients admitted to our hospital for ERCP preparation between January 2020 and February 2024.Patient baseline clinical data were collected using an electronic medical record system.Patients were randomly matched in a 1:4 ratio with data from 190 patients during the same period to establish a validation group(n=38)and a modeling group(n=152).Patients in the modeling group were divided into the gastric retention group(n=52)and non-gastric retention group(n=100)based on whether gastric retention occurred preoperatively.General data of patients in the validation group and identify factors influencing preoperative gastric retention in ERCP patients.A predictive model for preoperative gastric retention in ERCP patients was constructed,and calibration curves were used for validation.The receiver operating characteristic(ROC)curve was analyzed to evaluate the predictive value of the model.RESULTS We found no statistically significant difference in general data between the validation group and modeling group(P>0.05).The comparison of age,body mass index,hypertension,and diabetes between the two groups showed no statistically significant difference(P>0.05).However,we noted statistically significant differences in gender,primary disease,jaundice,opioid use,and gastrointestinal obstruction between the two groups(P<0.05).Mul-tivariate logistic regression analysis showed that gender,primary disease,jaundice,opioid use,and gastrointestinal obstruction were independent factors influencing preoperative gastric retention in ERCP patients(P<0.05).The results of logistic regression analysis revealed that gender,primary disease,jaundice,opioid use,and gastroin-testinal obstruction were included in the predictive model for preoperative gastric retention in ERCP patients.The calibration curves in the training set and validation set showed a slope close to 1,indicating good consistency between the predicted risk and actual risk.The ROC analysis results showed that the area under the curve(AUC)of the predictive model for preoperative gastric retention in ERCP patients in the training set was 0.901 with a standard error of 0.023(95%CI:0.8264-0.9567),and the optimal cutoff value was 0.71,with a sensitivity of 87.5 and specificity of 84.2.In the validation set,the AUC of the predictive model was 0.842 with a standard error of 0.013(95%CI:0.8061-0.9216),and the optimal cutoff value was 0.56,with a sensitivity of 56.2 and specificity of 100.0.CONCLUSION Gender,primary disease,jaundice,opioid use,and gastrointestinal obstruction are factors influencing preoperative gastric retention in ERCP patients.A predictive model established based on these factors has high predictive value.

A systematic review of the efficacy of traditional Chinese medicine retention enema in the treatment of ulcerative colitis

The objective of this study is to summarize the efficacy and safety of traditional Chinese medicine(TCM)enema in the treatment of ulcerative colitis(UC).The randomized controlled trials on TCM enema intervention in the treatment of UC were searched in seven databases:PubMed,Embase,Web of Science,CBM,CNKI,Wanfang Database,and VIP Database from January 1,2013 to June 6,2022,and the data were analyzed using RevMan 5.3 software.A total of 18 studies involving 1514 UC patients were included.Meta analysis results showed that compared with conventional Western medicine,Chinese medicine enema had a significant effect on UC,and the clinical effective rate of the experimental group using Chinese medicine enema was 4.45 times that of the control group using conventional Western medicine(odds ratio=4.45,95%confidence interval[3.27,6.06]).Therefore,Chinese medicine enema is effective in the treatment of UC,and can significantly reduce related symptoms.

High Urine Retention: Experience in a Series of Patients with Renal Failure Patients

Purpose: High urinary retention (HUR) can negatively impact renal function. Our study aimed to present the epidemiological, diagnostic, and therapeutic aspects of HUR in a Senegalese academic hospital. Patients and Methods: We conducted a retrospective study of 70 patients with HUR associated with renal failure from January 2017 to December 2020. Parameters examined included: age, sex, coexisting conditions affecting renal function, clinical symptoms, diagnostic tests, causes of HUR, urinary diversion, and patient outcomes. Results: The average age was 66, with a majority of male patients (87%). Twenty-three patients had pre-existing medical conditions. Oligo-anuria was the most common reason for detecting HUR (70%). Half of the patients had an ECOG score ≥ 2. The mean creatinine level was 50.7 mg/l. Nineteen patients exhibited hydroelectrolytic disorders. Bacterial colonization was observed in 25 patients. Ultrasound and computed tomography were the most frequently performed imaging tests (100% and 62.8%, respectively). Sixty-seven patients had ureterohydronephrosis (UHN), with bilateral UHN in 88.6% of cases. Pelvic cancers (47.1%) were the primary cause of HUR, primarily bladder cancers (27.1%). Nephrostomy was the most common urinary drainage method (50%), particularly for obstructions due to pelvic cancer (88.6%). The majority of patients (52.8%) regained normal renal function after drainage. Nineteen deaths occurred among elderly patients with compromised general health. Conclusion: Urinary drainage significantly improved renal function for most patients. Pelvic cancer emerged as the leading cause of HUR. Nephrostomy was the predominant drainage method.

Study on the Application Effect of Flexible Nursing Care in Patients with Postpartum Urinary Retention and its Effect on Lactation

Objective: To study the application effect of flexible nursing in patients with postpartum urinary retention and its effect on lactation. Methods: A total of 200 cases of postpartum urinary retention patients admitted between January 2021 and January 2022 were selected and randomly grouped into two groups, a control group (conventional nursing) and an observation group (flexible nursing), of 100 cases each. The time of onset of lactation, the lactation volume score, urinary indicators, the amount of post-partum hemorrhage, and the quality of life score of the two groups were compared. Results: The observation group’s lactation initiation time (21.41 ± 1.52) h and lactation volume score (2.11 ± 0.52) were better than that of the control group (P < 0.05). The observation group’s first urination time (2.11 ± 0.51) min was lower than the control group, while the urinary retention completely relieved time (33.12 ± 8.61) h, and first urinary volume (262.17 ± 52.41) mL was higher than that of the control group (P < 0.05). The amount of postpartum hemorrhage in the observation group (151.21 ± 22.12) mL was less than that in the control group (P < 0.05). The scores of somatic functioning (86.25 ± 2.20), psychological functioning (91.56 ± 1.45), social functioning (89.25 ± 2.45), and material life (89.75 ± 1.45) of the observation group were higher than those of the control group after nursing (P < 0.05). Conclusion: Flexible nursing care in patients with postpartum urinary retention exhibited significant nursing effects and lactation function was effectively improved.

Increased intron retention is linked to Alzheimer’s disease

Intron retention in aging and Alzheimer's disease (AD): AD is an age-related neurodegenerative disorder with pathological accumulation of amyloid plaque (Masters et al., 2015), which can be classified into familial and sporadic form. In familial AD, mutations in genes encoding either amyloid precursor protein or presenilin (PS1 and PS2) cause overproduction of amyloid-42 molecules and early onset of dementia.

Petroleum Retention,Intraformational Migration and Segmented Accumulation within the Organic-rich Shale in the Cretaceous Qingshankou Formation of the Gulong Sag,Songliao Basin,Northeast China

In this study,organic geochemical and petrological analyses were conducted on 111 shale samples from a well to understand the retention,intraformational migration and segmented accumulation(shale oil enrichment in different intervals is unconnected)features of shale oil within the organic-rich shale in the Qingshankou Formation of the Gulong Sag.Our study shows that retained petroleum characteristics in the investigated succession are mainly influenced by three factors:organic richness,intraformational migration and segmented accumulation.Organic matter richness primarily controls the amount of retained petroleum,especially the‘live’component indicated by the S_(2)value rather than the total organic carbon(TOC)figure alone.The negative expulsion efficiencies determined by mass-balance calculations of hydrocarbons reveal that petroleum from adjacent organic-rich intervals migrates into the interval of about 2386-2408 m,which is characterized by high free hydrocarbon(S_(1)),OSI and saturated hydrocarbons content,along with a greater difference inδ^(13)C values between polar compounds(including resins and asphaltenes)and saturated hydrocarbons.The depth-dependent heterogeneity of carbon isotope ratios(δ^(13)C)of mud methane gas,δ^(13)C of extracts gross composition(SARA),δ^(13)C of kerogen and SARA content of extracts suggest that the studied succession can be subdivided into four intervals.The shale oil sealing enrichment character in each interval is further corroborated by the distinctδ^(13)C values of mud methane gas in different intervals.Due to the migration of petroleum into the 2386-2408 m interval,the S_(1),OSI and saturated hydrocarbons content of the interval show higher relative values.The maturity of organic matter in the 2471-2500 m interval is at the highest with the smaller size molecular components of the retained petroleum.Thus,favorable‘sweet spots’may be found in the 2386-2408 m interval and the 2471-2500 m interval,according to the experiment results in this study.

How can we increase attraction and retention of nurses? A research with young nurses

The persistent challenges in attracting and retaining a diverse healthcare workforce, with a specific focus on nurses, have become increasingly pronounced in recent years. These hurdles have been exacerbated by a growing difficulty in retaining young nurses, thereby exacerbating labor shortages driven by demographic shifts and the retirement of experienced nursing professionals. While most research efforts have concentrated on the broader issue of nurse retention, our study is centered on a specific demographic—young nurses. Our research endeavors to shed light on the unique challenges faced by young nurses through a qualitative survey involving nursing students who are simultaneously employed. We seek to discern the multifaceted obstacles they encounter in both their academic environment and the healthcare organizations where they work. While certain challenges are linked to course organization, examinations, and the time required for studying, our respondents overwhelmingly emphasize the pivotal role of the work environment in facilitating the harmonization of work, family, and educational commitments. This reconciliation is achieved through measures such as flexible working arrangements and the efficient organization of nursing duties. The primary objective of our research is to provide insights into how these diverse challenges can be effectively addressed and how a range of measures can significantly contribute to the attraction and retention of nursing students, as well as the long-term retention of nurses within the healthcare system. Our recommendations are intended to be of practical use to a wide array of stakeholders, including academic institutions, particularly colleges and universities offering nursing programs, as well as hospitals, clinics, and other healthcare institutions that hire nurses. By collaboratively addressing these challenges and implementing the recommended measures, we aim to fortify the healthcare workforce and ensure the continued provision of quality care to patients. .

The Influence of Fabric Structural Parameters on Dust Retention Using a Simple, Newly Constructed Device

This article has been directed to Environment Protection Technology with the purpose of providing a new instrument designed and developed to measure filtration efficiency through the relationship between clean cloth fabric structural parameters, dust parameters, and test measuring variables. Fabric samples used throughout the present study were woven cotton 100%, polyester 100% and cotton/polyester 50/50%. The warp count: 30/2 for all fabric samples, the weft count is 9/1;12/1;and 20/1. The weave of satin, basket, and twill is 3/1 with four different picks/cm to produce the fabrics with the same cover factor. For dust separation fabrics range in weight from “300 - 450 g/m2” with an air permeability of “100 - 300 l/d m2·min” at “196.2 Pa” (20 mm WG) as specified in DIN 53887. Air permeability through fabrics depends entirely on the sieving percent of the surface of the fabric, which is partly the pores and partly the permeability through the yarns, which are the basic elements of a fabric. The results showed that dust capturing depends entirely on air permeability, which is related to fabric weave structure and fabric material at specified testing and measuring variables.

不同助留体系对装饰原纸二氧化钛复合填料留着效果的影响

本研究分别分析了单元助留体系阳离子聚酰胺环氧氯丙烷树脂(PAE),双元助留体系PAE/阴离子聚丙烯酰胺(APAM)、PAE/膨润土(MMT)、PAE/阳离子聚丙烯酰胺(CPAM),三元助留体系PAE/CPAM/APAM、PAE/CPAM/MMT对装饰原纸TiO_(2)/滑石粉复合填料留着效果的影响。确定了各个体系的最佳添加量,并筛选出助留效果最好的助留体系。结果表明,当PAE添加量2.0%、CPAM添加量0.08%、MMT添加量0.6%时,组成的PAE/CPAM/MMT三元助留体系留着效果最佳,此时,浆料和填料留着率分别为89.5%和65.0%,纸张的不透明度、白度及匀度分别为98.5%、86.6%和91。

黄精多糖提取工艺优化及其吸湿、保湿性能研究

目的:优化黄精多糖提取工艺,并研究其吸湿保湿性能、探索其护肤功能。方法:采用热回流法结合正交进行黄精多糖提取工艺优化;以黄精多糖为实验对象,采用重量法进行吸湿性(相对湿度分别为43%和81%)、保湿性试验,并对吸湿曲线进行模型拟合;将黄精多糖添加到市售面霜中进行皮肤水分测试。结果:实验表明黄精多糖最佳提取工艺条件为:料液比1∶25(g/mL),提取时间是150 min,提取次数为1次;黄精多糖在不同相对湿度下的吸湿均符合双指数模型;置于干硅胶环境下48 h,黄精多糖保湿率为14.00%。结论:黄精多糖具有良好的保湿性,在护肤品开发中具有一定的应用价值。

滇中地区主要森林凋落物有效截留量及其影响因素

[目的]研究滇中地区主要森林类型凋落物有效截留量差异及其影响因素,为该地未来植被恢复和森林管理提供科学支撑。[方法]利用样方调查法、烘干法和浸泡法分析了昆明市双河—磨南德水源林自然保护区内的地带性次生常绿阔叶林(SF)、针阔混交林(TF)、旱冬瓜林(ACF)、华山松林(PA)、成熟云南松林(OPY)、幼龄云南松林(YPY)、银荆林(AD)7种主要森林类型凋落物未分解层(OL)和半分解层(OF)的有效截留量及其影响因素。[结果](1) 7种森林类型凋落物厚度为2.06~7.57 cm,其中OL层1.10~4.30 cm, OF层0.83~3.93 cm,凋落物层厚度排序为TF>ACF>PA>OPY>SF>AD>YPY,凋落物蓄积量为4.75~17.45 t/hm^(2),其中OL层占比为32.02%~62.48%,OF层占比为37.52%~67.98%,凋落物层蓄积量排序为TF>PA>SF>OPY>ACF>AD>YPY。(2)凋落物最大持水率为115.3%~170.7%,初始吸水速率为3.65~5.62 g/(g·h),凋落物持水率和浸水时间的关系可用对数函数y=aln(x)+b表示,吸水速率与浸水时间可用幂函数y=kt~n表示。(3)凋落物最大持水量为7.05~32.19 t/hm^(2),排序为PA>TF>SF>OPY>ACF>AD>YPY,有效截留量为5.45~25.34 t/hm^(2),以TF最高(25.34t/hm^(2)),PA(24.99 t/hm^(2))和SF(24.62 t/hm^(2))次之,YPY最低(5.45t/hm^(2)),影响有效截留量的场地特征主要为蓄积量、自然含水率、分解强度以及厚度,影响有效截留量的水动力过程主要是最大失水量、前6.0 h平均吸水速率和前2.0 h平均吸水速率。[结论]滇中地区未来植树造林应以针阔混交林为主,注重对地带性次生常绿阔叶林的保育,采取辅助自然更新的措施促进人工纯林向混交林演化,同时减少踩踏、收集凋落物等人类活动的干扰,从而增加森林生态系统凋落物的水源涵养能力。

益心泰颗粒对慢性心衰(心气虚兼血瘀水停证)大鼠AMPK、PGC-1α的影响

目的观察益心泰颗粒对慢性心力衰竭(CHF)(心气虚兼血瘀水停证)大鼠AMPK、PGC-1α的影响。方法采用丙硫氧嘧啶灌胃及阿霉素腹腔注射复制CHF(心气虚兼血瘀水停证)模型大鼠,将造模成功大鼠分为模型组与益心泰低、中、高剂量组和曲美他嗪组;另设正常对照组。灌胃4周后观察心肌组织病理结构,检测LVEF、血清NT-proBNP及心肌组织FFA、ATP/AMP、LAC、pAMPK、AMPK、PGC-1α水平。结果与模型组比较,益心泰颗粒低、中、高剂量组及曲美他嗪组LVEF、心肌组织ATP/AMP值、心肌组织p-AMPK、AMPK、PGC-1α相对灰度值均明显升高(P<0.05或P<0.01),血清NT-proBNP及心肌组织FFA、LAC明显降低(P<0.05或P<0.01)。结论益心泰颗粒能促进慢性心力衰竭(心气虚兼血瘀水停证)大鼠AMPK、PGC-1α表达,改善心肌能量代谢。