Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis

Superoxide dismutase（SOD） is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD（SOD1） and Mn-SOD（SOD2）, in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature（18–34°C） and copper ion concentration（0.1–1.5 mg/L） on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant（P〈0.01）, the quadratic effect of temperature was significant（P〈0.05）; the linear effect of copper ion concentration was not significant（P〉0.05）, while the quadratic effect of copper ion concentration was highly significant（P〈0.01）; the interactive effect of temperature and copper ion concentration was not significant（P〉0.05）; the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.

Analysis of the linkage positions and isomers of monosaccharide units in oligosaccharides by negative secondary ion mass spectrometry in combination with the stereoselective reaction with substituted boronic acid

The negative secondary ion mass spectrometry,in combination with the stereoselective derivatizations with substituted boronic acid RB(OH)_2,was used in the analysis of fourteen oligosac- charides.The mass spectra of the derivatives provide information on their linkage Positions and iso- merism of the individual monoscaccharide units.The results indicated that among the derivatives of the oligosaccharides analyzed,those with 1—4 and 1—6 linkages all presented the ion peaks at m/z 287,sometimes one more peak at m/z 449.Furthermore,a relationship was found between the linkage positions and the intensity orders of the derivative ions.Finally,the derivatives of the disaccharides with a galactose presented an intense ion peak at m/z 347,and those of oligosaccharides with 1—6 linkage to a galactose at terminal presented the ion at m/z 317.In the case of oligosaccharides with a fructose residue,characteristic ion of m/z 155 was produced.The conditions of stereoselective derivatizations and mass spectrometry were studied,in order to obtain a better reproducibility of the mass spectra.

Energy dependence of resonance production in relativistic heavy ion collisions

The production of the hadronic resonances K^＊0（892）,φ（1020）,∑^＊（1385）,and Ξ^＊（1530） in central AA collisions at √SNN=17.3,200,and 2760 GeV is systematically studied.The direct production of these resonances at system hadronization is described by the quark combination model and the effects of hadron multiple-scattering stage are dealt with by a ultra-relativistic quantum molecular dynamics model（UrQMD）.We study the contribution of these two production sources to final observation and compare the final spectra with the available experimental data.The pT spectra of K^＊0（892） calculated directly by quark combination model are explicitly higher than the data at low pT≤1.5 GeV,and taking into account the modification of rescattering effects,the resulting final spectra well agree with the data at all three collision energies.The rescattering effect on φ（1020） production is weak and including it can slightly improve our description at low p_T on the basis of overall agreement with the data.We also predict the pT spectra of ∑^＊（1385） and Ξ^＊（1530）,to be tested by the future experimental data.

Multiplicity fluctuation and correlation of mesons and baryons in ultra-relativistic heavy-ion collisions at the LHC

We study the multiplicity fluctuation and correlation of identified mesons and baryons formed at hadronization by the quark combination mechanism in the context of ultra-relativistic heavy-ion collisions. Based on the statistical method of free quark combination, we derive the two-hadron multiplicity correlations, including meson-meson and meson-baryon correlations, and take the effects of quark number fluctuation at hadronization into account by a Taylor expansion method. After including the decay contributions, we calculate the dynamical fluctuation observable ν_（dyn） for Kπ, pπ and Kp pairs and discuss what underlying physics can be obtained by comparing with data from Pb-Pb collisions at sNN^（1/2）=2.76 Te V and simulations from the HIJING and AMPT event generators.