期刊文献+
共找到12,205篇文章
< 1 2 250 >
每页显示 20 50 100
Influence of nitrogen status on fermentation performances of non-Saccharomyces yeasts:a review 被引量:1
1
作者 Jinchen Li Mengmeng Yuan +3 位作者 Nan Meng Hehe Li Jinyuan Sun Baoguo Sun 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期556-567,共12页
Nitrogen,one of the most crucial nutrients present in grapes and musts,plays a key role in yeast activities during alcoholic fermentation.Such influences are imposed on yeast growth and fermentation performances inclu... Nitrogen,one of the most crucial nutrients present in grapes and musts,plays a key role in yeast activities during alcoholic fermentation.Such influences are imposed on yeast growth and fermentation performances including the formation of secondary metabolites.Saccharomyces cerevisiae,the main yeast responsible for fermentation,has been studied extensively regarding nitrogen impacts.On the other hand,a similar study for non-Saccharomyces yeasts,whose contributions to winemaking have gradually been acknowledged,remains to be fully explored,with a few studies being reported.This review starts by discussing nitrogen impacts on non-Saccharomyces yeast growth and fermentation kinetics in different case scenarios,then proceeds to summarize the nitrogen preferences of individual yeast strains with regulation mechanisms elucidated by recent studies.Detailed discussions on the influences on the production of volatile compounds and proposed pathways therein are made,followed by future work suggested as the final section.In summarizing the nitrogen impacts on non-Saccharomyces yeasts throughout alcoholic fermentation,this review will be helpful in obtaining a more comprehensive view on these non-conventional wine yeasts in terms of nutrient requirements and corresponding volatile production.Research gaps will therefore be elucidated for future research. 展开更多
关键词 Non-Saccharomyces yeasts NITROGEN Fermentation kinetics Nitrogen preference Wine aroma
下载PDF
Improving Semi-Dried Brown Rice Noodle Quality via Mixed Fermentation of Lactobacillus and Yeast
2
作者 LUO Lijuan CHENG Zixuan +6 位作者 QIAO Fan XIONG Gangping LIU Jun HUANG Qingming LI Jiangtao LIN Qinlu LIU Chun 《Rice science》 SCIE CSCD 2024年第5期489-493,I0001-I0005,共10页
To address the coarse texture and poor cooking quality of brown rice flour,we employed fermentation using lactobacillus and yeast in varying proportions.The fermented flour from early indica rice Pear 13 was then proc... To address the coarse texture and poor cooking quality of brown rice flour,we employed fermentation using lactobacillus and yeast in varying proportions.The fermented flour from early indica rice Pear 13 was then processed into semi-dried brown rice noodles. 展开更多
关键词 DRIED COOKING yeast
下载PDF
Effect of β-Glucan (Angel Yeast) Compared to a Placebo on Cold and Flu Incidence and Symptoms in an Adult Population—A Double Blind, Randomised Controlled Trial
3
作者 David Briskey Haibo Zhang +1 位作者 Zhixian Chen Amanda Rao 《Food and Nutrition Sciences》 CAS 2024年第6期484-497,共14页
Background: 1-3, 1-6 β-glucan derived from Baker’s yeast (Saccharomyces cerevisiae) has been widely studied for its immune stimulatory capabilities and safety. Previous studies found β-glucan to have efficacy at re... Background: 1-3, 1-6 β-glucan derived from Baker’s yeast (Saccharomyces cerevisiae) has been widely studied for its immune stimulatory capabilities and safety. Previous studies found β-glucan to have efficacy at reducing incidence of URTIs as well as being a low risk for negative side effects. The current study aimed to examine the effects of yeast β-glucan (Angel Yeast) on cold and flu incidences and symptoms in healthy adults. Methods: Two hundred and thirty-one males and females aged 18 to 65 years old supplemented with either β-glucan or a placebo for 3-months. Participants completed a general health questionnaire every 4 weeks and in addition, if participants experienced any cold or flu symptoms, these were recorded daily (along with severity) until resolved or up to 2 weeks. Results: Supplementation with β-glucan reduced the self-reported severity of sore throats and improved sleep quality compared to the placebo group. Conclusions: Yeast β-glucan supplementation appears to be able to help reduce certain symptoms experienced during a cold or flu episode and is safe and well tolerated. 展开更多
关键词 BETA-GLUCAN COLD FLU Baker’s yeast
下载PDF
Mathematical Modeling of Cell Polarity Establishment of Budding Yeast
4
作者 Yue Liu Jun Xie +1 位作者 Hay-Oak Park Wing-Cheong Lo 《Communications on Applied Mathematics and Computation》 EI 2024年第1期218-235,共18页
The budding yeast Saccharomyces cerevisiae is a powerful model system for studying the cell polarity establishment.The cell polarization process is regulated by signaling molecules,which are initially distributed in t... The budding yeast Saccharomyces cerevisiae is a powerful model system for studying the cell polarity establishment.The cell polarization process is regulated by signaling molecules,which are initially distributed in the cytoplasm and then recruited to a proper location on the cell membrane in response to spatial cues or spontaneously.Polarization of these signaling molecules involves complex regulation,so the mathematical models become a useful tool to investigate the mechanism behind the process.In this review,we discuss how mathematical modeling has shed light on different regulations in the cell polarization.We also propose future applications for the mathematical modeling of cell polarization and morphogenesis. 展开更多
关键词 Budding yeast CDC42 MORPHOGENESIS SEPTIN Mathematical models
下载PDF
Impact of Storage Temperature on Microbial Diversity and Probiotic Effect of Liquid Brewers’ Yeast
5
作者 Peter Alphonce Obuong Alaru Alfred Anakalo Shitandi +1 位作者 Symon Maina Mahungu John Muasya Kilumba Muia 《Open Journal of Animal Sciences》 2024年第3期168-182,共15页
Using probiotics as animal feed additives instead of antibiotics is gaining momentum to avert adverse negative effects on human health. Liquid brewers yeast (LBY) is an industrial by-product containing probiotic micro... Using probiotics as animal feed additives instead of antibiotics is gaining momentum to avert adverse negative effects on human health. Liquid brewers yeast (LBY) is an industrial by-product containing probiotic microorganisms and is also used as a protein supplement for dairy animals. Nevertheless, value chain actors lack of appropriate handling practices compromises the by-products quality and safety. This study aimed to determine the effect of variation in temperature on microbial diversity and probiotic effects during the storage time of LBY sampled from distributors and farmers from Githunguri sub-county of Kenya. The samples were stored at 20C, 25C and 30C, then tested on 0, 5, 10, 15 and 20 days. The studys parameters involved determining the pH levels, lactic acid bacteria (LAB), total coliform count (TCC), mould, and yeast in LBY. The rate (k) of the reaction kinetics model was used to extrapolate the expected probiotic shelf life. The LAB and yeast populations were reduced in a first-order reaction at all storage temperatures. The rate of reduction in the numbers of LAB reduced with an increase in temperature (k = 0.019 and 0.023) at 20C and 30C, respectively. Yeasts highest rate of growth reduction was 25C (k = 0.009) and least at 30C (k = 0.043). The minimum effective concentration for probiotics of 106 CFU/mL needed to observe the beneficial physiological impact on farm animals was achieved between 34.9 and 35.5 days at the tested storage temperatures. The study provides insight into the unexploited low-cost probiotic potential of LBY in dairy production. Conversely, handling practices and environmental microbial contamination along the value chain can compromise product quality and safety. There is a need to advocate its use in dairy for improved productivity and sensitize farmers to appropriate hygienic measures along the LBY value chain. 展开更多
关键词 Liquid Brewers’ yeast Microbial Diversity PROBIOTICS Shelf Life
下载PDF
Protective Effect of Dietary Yeast Cultures on Lipopolysaccharide-induced Oxidative Stress,Immune and Inflammatory Responses in Pseudobagrus ussuriensis
6
作者 Tao Shengqiang Yang Zhangziyu +6 位作者 Wang Yi Zhao Xu Wang Shuai Wang Jiahao Yang Yuhong Yang Chenghui Cui Cunhe 《Journal of Northeast Agricultural University(English Edition)》 CAS 2024年第4期74-85,共12页
This study aimed to evaluate the effects of dietary yeast culture(YC)on lipopolysaccharide(LPS)-induced oxidative stress,immune and inflammatory response in P.ussuriensis.The fish were randomly assigned into three gro... This study aimed to evaluate the effects of dietary yeast culture(YC)on lipopolysaccharide(LPS)-induced oxidative stress,immune and inflammatory response in P.ussuriensis.The fish were randomly assigned into three groups as the control group,LPS group and YC+LPS group.The fish in the control were fed diet with no YC supplementation and no LPS challenge,and the fish in the LPS group or YC+LPS group were fed diet supplemented with no YC or 20 g·kg^(-1)YC,and with LPS challenge,respectively.The results showed that compared with the control group,intestinal total antioxidant capacity(T-AOC)level and superoxide dismutase(SOD)activity were significantly decreased,while intestinal malondialdehyde(MDA),plasma aspartate transaminase(AST)and alanine transaminase(ALT)levels were significantly increased in the LPS group(P<0.05).Besides,lower plasma alkaline phosphatase(ALP),alternative complement pathway(ACH50)activity and the albumin(ALB)level,as well as higher lysozyme(LZM)activity,were also found in the LPS group.However,dietary 20 g·kg^(-1)YC supplementation could relieve the above LPS-induced changes in Pseudobagrus ussuriensis.Furtherly,LPS challenge could significantly up-regulate gene expression of interleukin-8(IL-8),heat shock protein(HSP70)and NF-κBp65 except for toll-like receptors 2(TLR2),while dietary 20 g·kg^(-1)YC supplementation suppressed the increased expression of NF-κBp65 and IL-8 induced by LPS in P.ussuriensis.In summary,LPS challenge could induce immune impairment,oxidative stress and hepatic damage,and the protective effect of dietary 20 g·kg^(-1)YC supplementation on LPS-induced immune impairment and oxidative stress was observed in the present study,which was associated with the enhanced levels of antioxidant enzymes and immune parameters.Also,dietary 20 g·kg^(-1)YC supplementation could suppress LPS-induced inflammatory response by down-regulating NF-κBp65,HSP70 and IL-8 gene expression. 展开更多
关键词 yeast culture LIPOPOLYSACCHARIDE NF-ΚBP65 Pseudobagrus ussuriensis
下载PDF
Microbiological Quality of “Rabilé”, a Yeast Used for Fermentation of Dolo, a Local Beer in Dédougou, Burkina Faso
7
作者 Amana Metuor Dabiré Nicolas Ouédraogo +1 位作者 Cheick Alassane Djibila Damis Yves Patrik Bouniounou 《Open Journal of Applied Sciences》 2024年第4期849-864,共16页
Sorghum beer or dolo is part of the eating habits of part of the population of Dédougou because of its low price compared with industrial beers. Its production is an ancestral tradition that uses traditional equi... Sorghum beer or dolo is part of the eating habits of part of the population of Dédougou because of its low price compared with industrial beers. Its production is an ancestral tradition that uses traditional equipment and gives dolo organoleptic properties that are not found in industrial beers. The production process involves several stages, including fermentation, which itself comprises natural lactic fermentation followed by alcoholic fermentation using traditional yeasts, which are not controlled in any way. The general aim of this study is to assess the microbiological quality of these fermentative yeasts in the town of Dédougou, in order to contribute to the health safety of the population and the promotion of these local beers. Twenty samples of fermenting yeast were analyzed according to ISO standards, to isolate enterobacteria, total and faecal coliforms according to standard procedures for isolating these micro-organisms. The isolated strains were identified using the API20E gallery. Microbiological analyses revealed the presence of 51.17% enterobacteria, 45.38% total coliforms and 3.45% thermotolerant coliforms. We counted 40% Escherichia coli, 20% Enterobacter cloacae, 20% Klebsiella pneumoniae and 20% Klebsiella spp. All the strains detected are capable of surviving in hostile conditions and could harm the quality of the dolo, consumer health and cause real collective food poisoning in the town of Dédougou. This enabled us to assess the microbial quality of these yeasts and to propose more suitable measures for producing and preserving dolo under hygienic conditions to protect consumer health. 展开更多
关键词 Dolo yeast Rabilé Microbiological Quality BACTERIA
下载PDF
Construction of a Yeast Hybrid Library and Identification of Proteins Regulating CaABI3/VP1-1 Expression in Capsicum annuum var.conoides
8
作者 Huiru Deng Huan Tian +4 位作者 Liuyan Yang Siyang Ou Hui Wang Guangdong Geng Suqin Zhang 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第12期3273-3291,共19页
Hot pepper(Capsicum annuum var.conoides)is a significant vegetable that is widely cultivated around the world.Currently,global climate change has caused frequent severe weather events,and waterlogging stress harms the... Hot pepper(Capsicum annuum var.conoides)is a significant vegetable that is widely cultivated around the world.Currently,global climate change has caused frequent severe weather events,and waterlogging stress harms the pepper industry by affecting the planting period,growth conditions,and disease susceptibility.The gene CaABI3/VP1-1 could improve pepper waterlogging tolerance.In order to explore the upstream regulatory mechanism of CaABI3/VP1-1,a high-quality standardized yeast hybrid library was successfully constructed for yeast one-,two-,and threehybrid screening using pepper‘ZHC2’as the experimental material,with a library recombinant efficiency of up to 100%.The length of inserted fragments varied from 650 to 5000 bp,the library titer was 5.18×10^(6)colony-forming units(CFU)·mL-1,and the library capacity was 1.04×10^(7)CFU of cDNA inserts.The recombinant bait plasmid was used to successfully identify 78 different proteins through the yeast one-hybrid system,including one transcription factor within the ethylene-responsive factor family and the other within the growth-regulating factor family.The interaction happened between LOC124895848 and CaABI3/VP1-1 promoter by point-to-point yeast one-hybrid experiment.The expression level of the 12 selected protein-coding genes was then evaluated by quantitative real-time polymerase chain reaction.Results indicated the protein coding genes showed different responses to waterlogging stress and that the activity of the CaABI3/VP1-1 promoter could be inhibited or activated by up-regulating or down-regulating gene expression,respectively.The identification of these proteins interacting with the promoter provides a new perspective for understanding the gene regulatory network of hot pepper operating under waterlogging stress and provides theoretical support for further analysis of the complex regulatory relationship between transcription factors and promoters. 展开更多
关键词 Capsicum annuum var.conoides CaABI3/VP1-1 PROMOTER yeast one-hybrid interacting protein waterlogging stress
下载PDF
Immobilization techniques for beverage production using yeast cell systems: challenges, types, and future perspectives - a mini review
9
作者 Syed Sib Tul Hassan Shah Iqra Naeem +2 位作者 Aimen Naeem Nabeel Khalid Bhutta Fatima Noor 《Food and Health》 2024年第4期27-38,共12页
Yeast immobilization is a process of physical entrapment of yeast cells using different techniques while maintaining their biological activity.Continuous fermentation systems have significant advantages over conventio... Yeast immobilization is a process of physical entrapment of yeast cells using different techniques while maintaining their biological activity.Continuous fermentation systems have significant advantages over conventional methods.Research highlights that immobilized yeast cell systems have several benefits as compared to free yeast cells.The immobilized yeast cell systems improve fermentation rates,especially when paired with continuous fermentation and appropriate immobilization techniques.Understanding various immobilization techniques,continuous fermentation processes,yeast metabolic activity related to beverage flavor production,and bioreactor designs is vital for optimizing the use of immobilized yeast cells systems on industrial scale.This review provides an overview of recent basic research on immobilized yeast cell systems,with a focus on continuous beverage fermentation.In this study,different reactor configurations and immobilization techniques are explored.The study focus on the impacts of immobilization on the yeast cells,and discuss the recent advancements in these techniques.The review concludes with a discussion on the practical applications of immobilized yeast cells and continuous fermentation in beverage production. 展开更多
关键词 yeast Cell System Beverages Continuous Fermentation Microbial System
下载PDF
De novo biosynthesis of phytohormone jasmonates in engineered yeast
10
作者 Chengfeng Xue Meng Zhang Ruifeng Yao 《Advanced Agrochem》 2024年第1期4-5,共2页
The plant defense hormone jasmonates not only play important roles in plant growth,development,and resistance,but also hold promise for bringing new strategies in plant protection and cancer therapy.Recently,de novo b... The plant defense hormone jasmonates not only play important roles in plant growth,development,and resistance,but also hold promise for bringing new strategies in plant protection and cancer therapy.Recently,de novo biosynthesis of natural and unnatural jasmonates in refactored yeast with integration of 15 heterologous genes and 3 native genes deleted was reported.Here,we highlight the feasible and sustainable platform to efficiently produce jasmonates,which would benefit both agriculture and human health. 展开更多
关键词 Plant hormone JASMONATE de novo synthesis yeast
下载PDF
Compound Danshen Yeast 1.0
11
作者 Rongsheng Li Jie Wang +1 位作者 Yuanyuan Han Zhubo Dai 《Science of Traditional Chinese Medicine》 2024年第4期303-311,共9页
Background:Heterologous synthesis presents a promising new approach for accessing the active ingredients of traditional Chinese medicine(TCM),contributing to the conservation of natural medicinal resources.Compound Da... Background:Heterologous synthesis presents a promising new approach for accessing the active ingredients of traditional Chinese medicine(TCM),contributing to the conservation of natural medicinal resources.Compound Danshen preparation,a widely used TCM formulation,is designed to treat coronary heart disease and angina pectoris.It is primarily composed of Salviae Miltiorrhizae Radix et Rhizoma(Danshen),Notoginseng Radix et Rhizoma(Sanqi),and borneol(Bingpian).Danshen primarily yields tanshinones and phenolic acids,while Sanqi produces notoginsenosides.Borneol serves as an auxiliary agent to promote mental clarity,dissipate heat,and relieve pain.Objectives:The objective is to employ heterologous synthesis in a single yeast strain to produce the active ingredients of Compound Danshen preparation,and these include notoginsenosides,tanshinones,and borneol.Methods:Firstly,the“plug-and-play”terpene synthase screening framework in Saccharomyces cerevisiae was used for the identification of a novel,highly efficient bornyl diphosphate synthase.Furthermore,by leveraging the Compound Danshen preparation as a basis to concurrently reconstruct the biosynthetic pathways for the notoginsenoside precursor protopanaxadiol(a triterpene),the tanshinone precursor miltiradiene(a diterpene),and borneol(a monoterpene)within a single yeast strain.Results:This engineered strain,termed Compound Danshen Yeast 1.0,successfully produced protopanaxadiol at 62.34 mg/L,miltiradiene at 15.38 mg/L,and borneol at 2.54 mg/L in shake-flask fermentation.Conclusions:This research signifies the inaugural cross-species andmulticomponent synthetic biology endeavor that enables the synthesis of active ingredients in engineered cells,setting the stage for the industrial manufacture of TCM compounds. 展开更多
关键词 Heterologous biosynthesis Compound Danshen preparation Synthetic biology Saccharomyces cerevisiae Compound Danshen yeast 1.0
原文传递
产油酵母的研究现状与展望
12
作者 乔代蓉 冉雨鹭 李维 《四川师范大学学报(自然科学版)》 CAS 2025年第1期1-14,F0002,共15页
生物能源是从生物来源的材料制成的可再生能源,主要分为生物质能、生物燃料、生物气体三大类,具有绿色、低碳、清洁、可再生等优点.微生物油作为第三代生物柴油,有可能成为传统化石燃料的绿色替代品,是缓解能源挑战和环境问题的研究热点... 生物能源是从生物来源的材料制成的可再生能源,主要分为生物质能、生物燃料、生物气体三大类,具有绿色、低碳、清洁、可再生等优点.微生物油作为第三代生物柴油,有可能成为传统化石燃料的绿色替代品,是缓解能源挑战和环境问题的研究热点.本文综述产油酵母油脂合成与调控机制以及代谢工程等研究进展.产油酵母作为微生物油生产的主体,其异柠檬酸脱氢酶的活性依赖一磷酸腺苷(AMP),使得它们在限氮条件下大量积累油脂;酵母积累油脂受到各种条件的影响(比如碳氮源、温度、溶氧、pH值等),相关碳氮源利用与胁迫调控转录因子GAT1、MIG1、ASG1、MYB、GRAS、CBF11、TORC1 bHLH8和PHD等也调控其脂代谢.但产油酵母相对于酿酒酵母来说,其油脂合成机制与遗传背景依旧不清晰,限制了其工业化应用.因此,未来还需要加强微生物油生产技术的研究,从提高菌株鲁棒性、增加经济性代谢副产物产量、提高廉价底物利用能力等方面降低微生物油的生产成本,使其成为传统化石燃料的有效替代品. 展开更多
关键词 生物柴油 产油酵母 油脂合成 调控机制 代谢工程 经济性
下载PDF
酵母水解物对哺乳犊牛生长性能、血清抗氧化指标和瘤胃菌群的影响
13
作者 李元晓 刘丹 +5 位作者 晏克灿 孙成振 孙娟娟 皮庆洁 鲁志民 张才 《动物营养学报》 北大核心 2025年第1期387-399,共13页
本试验旨在探讨补饲酵母水解物对哺乳犊牛生长性能、血清抗氧化指标及瘤胃菌群的影响。试验选取30头1日龄、健康、体重[(39±2)kg]相近的荷斯坦母犊牛,随机分为对照组(不补饲酵母水解物)和试验组(每头犊牛每天补饲5 g酵母水解物),每... 本试验旨在探讨补饲酵母水解物对哺乳犊牛生长性能、血清抗氧化指标及瘤胃菌群的影响。试验选取30头1日龄、健康、体重[(39±2)kg]相近的荷斯坦母犊牛,随机分为对照组(不补饲酵母水解物)和试验组(每头犊牛每天补饲5 g酵母水解物),每组15个重复,每个重复1头牛。试验期为63 d。记录犊牛体重和采食量,采集血清和瘤胃液样品,测定血清抗氧化指标和瘤胃菌群多样性。结果显示:1)补饲酵母水解物显著增加了犊牛14和28日龄的胸围以及28和42日龄的体斜长(P<0.05),但对生长性能和其他体尺指标未产生显著影响(P>0.05)。2)63日龄时,试验组血清中丙二醛(MDA)含量显著低于对照组(P<0.05),总抗氧化能力(T-AOC)显著高于对照组(P<0.05);7日龄时,试验组血清谷胱甘肽过氧化物酶(GSH-Px)活性显著高于对照组(P<0.05)。3)补饲酵母水解物使犊牛瘤胃菌群的Alpha多样性指数(Chao1和Observed-species指数)显著降低(P<0.05);同时,Beta多样性也发生了显著变化(P<0.05)。30日龄时,试验组瘤胃菌群中互养菌门(Synergistota)的相对丰度显著低于对照组(P<0.05),而放线菌门(Actinobacteriota)的相对丰度显著高于对照组(P<0.05);试验组瘤胃菌群中理研菌科RC9肠道群(Rikenellaceae_RC9_gut_group)的相对丰度在60日龄时显著低于对照组(P<0.05),而解琥珀酸菌属(Succiniclasticum)和普雷沃氏菌属7(Prevotella_7)的相对丰度在30日龄时显著高于对照组(P<0.05)。综上可知,在本试验条件下,每天每头补饲5 g酵母水解物对哺乳犊牛整体生长性能无显著影响,但显著提高了犊牛的抗氧化能力,并调节了瘤胃菌群结构,有助于改善犊牛健康状况。 展开更多
关键词 酵母水解物 哺乳犊牛 生长性能 抗氧化指标 瘤胃菌群
下载PDF
蜂蜜中嗜渗酵母菌TaqMan实时荧光定量PCR方法的建立
14
作者 姜玲玲 罗文菊 +3 位作者 雷露 周景瑞 艾蓉 余波 《贵州农业科学》 2025年第1期70-75,共6页
【目的】建立蜂蜜中嗜渗酵母菌的快速检测方法,为保障蜂蜜品质提供理论基础。【方法】根据GenBank中嗜渗酵母菌26S rDNA D_(1)~D_(2)基因序列设计特异性引物,PCR扩增获得嗜渗酵母D_(1)~D_(2)基因片段,构建重组质粒pMD-18T-JM为阳性标准... 【目的】建立蜂蜜中嗜渗酵母菌的快速检测方法,为保障蜂蜜品质提供理论基础。【方法】根据GenBank中嗜渗酵母菌26S rDNA D_(1)~D_(2)基因序列设计特异性引物,PCR扩增获得嗜渗酵母D_(1)~D_(2)基因片段,构建重组质粒pMD-18T-JM为阳性标准品,对荧光定量PCR反应条件进行优化,建立嗜渗酵母菌TaqMan探针实时荧光定量PCR方法,并对160份蜂蜜样品进行验证。【结果】建立的蜂蜜中嗜渗酵母菌TaqMan探针实时荧光定量PCR检测方法特异性好,灵敏度达0.004 pg/μL。TaqMan实时荧光定量PCR检测阳性率达98.5%,比普通PCR检测(阳性率84.1%)高14.4百分点,检测敏感性达100%。【结论】嗜渗酵母菌TaqMan探针实时荧光定量PCR方法特异、灵敏、快速、重复性好,适合于蜂蜜中嗜渗酵母菌的快速检测。 展开更多
关键词 蜂蜜 嗜渗酵母菌 探针 荧光定量 PCR
下载PDF
生物胁迫下黄瓜酵母双杂交cDNA文库构建与鉴定
15
作者 刘东 潘春清 张艳菊 《安徽农业科学》 2025年第2期93-96,108,共5页
以黄瓜抗病高代自交系D9320为试验材料,采用Gateway技术构建了黄瓜霜霉病和棒孢叶斑病菌胁迫下黄瓜酵母双杂交cDNA文库。结果表明:酵母文库克隆数为350个,文库滴度为3.5×10^(7)CFU/mL,重组率达到100%,插入片段长度在750~2000bp,平... 以黄瓜抗病高代自交系D9320为试验材料,采用Gateway技术构建了黄瓜霜霉病和棒孢叶斑病菌胁迫下黄瓜酵母双杂交cDNA文库。结果表明:酵母文库克隆数为350个,文库滴度为3.5×10^(7)CFU/mL,重组率达到100%,插入片段长度在750~2000bp,平均片段长度超过1000bp。文库容量和重组率及插入片段大小均说明霜霉病菌和棒孢叶斑病菌胁迫下黄瓜酵母双杂交cDNA文库构建成功,可用于黄瓜双抗机制互作蛋白的筛选。 展开更多
关键词 黄瓜霜霉病 黄瓜棒孢叶斑病 CDNA文库 酵母双杂交
下载PDF
以分子生物学鉴定结果为“金标准”评估Rapid ID Yeast Plus及API20C AUX对酵母样真菌的鉴定效能 被引量:4
16
作者 范欣 王贺 +8 位作者 苏霄翔 肖盟 马雪菲 马超越 刘旭光 马然 吴玥 陈艳钊 徐英春 《中国真菌学杂志》 CSCD 2013年第5期274-280,共7页
目的以分子生物学方法为"金标准"对两种商品化酵母样真菌鉴定产品Rapid ID Yeast Plus(简称RapID YST)及API20C AUX(简称API20C)的鉴定效能进行评估。方法从2010年中国医院侵袭性真菌感染监测网菌株库中筛选酵母样真菌25种,共... 目的以分子生物学方法为"金标准"对两种商品化酵母样真菌鉴定产品Rapid ID Yeast Plus(简称RapID YST)及API20C AUX(简称API20C)的鉴定效能进行评估。方法从2010年中国医院侵袭性真菌感染监测网菌株库中筛选酵母样真菌25种,共计194株。其中,包含临床最常见的5种酵母样真菌(白念珠菌、热带念珠菌、光滑念珠菌、近平滑念珠菌、新生隐球菌)共130株,占研究总菌株数的67.0%。所有菌株已经过分子生物学方法准确鉴定至种水平。菌株复苏分纯后,严格按照产品操作指南,平行进行RapID YST和API20C鉴定。结果所研究菌株中,有181株(18种)在RapID YST鉴定菌种数据库中,所有在库菌株种及复合体鉴定正确率为87.8%(159/181)。相比,API鉴定菌种库包含菌株174株(18种),在库菌株种及复合体鉴定正确率为92.0%(160/174)。RapID YST与API20C对于5种临床常见的酵母样真菌的种鉴定正确率分别为93.1%和97.1%。对于库外菌株,RapID YST的鉴定错误率分别为23.1%(3/13),相比API20C的鉴定错误率为60.0%(12/20)。综合此次评估结果,二者对酵母样真菌的鉴定效能无显著差异(McNemar检验,P>0.05)。结论两种商品化产品对酵母样真菌的鉴定效能基本一致;相较而言,RapID YST在操作便捷性、检测时间方面具有较大优势。 展开更多
关键词 侵袭性真菌病 酵母样真菌 RAPID ID yeast PLUS API20C AUX 分子生物学鉴定
下载PDF
Yeast基因下游二级结构与多聚腺苷作用信号 被引量:3
17
作者 张静 石秀凡 刘次全 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2001年第4期523-527,共5页
形成真核生物mRNA 3′末端的多聚腺苷 (poly (A) )作用涉及前体mRNA下游的三个元件 :效率元件(EE)、定位元件 (PE)以及实际的剪切和 poly (A)作用位点 ,实验研究提出了一些EE和PE的碱基序列组成 .对 180个Yeast基因下游 (终止密码子后 2... 形成真核生物mRNA 3′末端的多聚腺苷 (poly (A) )作用涉及前体mRNA下游的三个元件 :效率元件(EE)、定位元件 (PE)以及实际的剪切和 poly (A)作用位点 ,实验研究提出了一些EE和PE的碱基序列组成 .对 180个Yeast基因下游 (终止密码子后 2 0 0个碱基 )二级结构进行的详细分析显示 ,约 86 %的EE、 89%的PE与二级结构中碱基非配对的环 (发夹环、膨胀环、内环或多分支环 )区或连接单链区有关 .这个结果提示 ,反式因子对EE和PE的识别和作用在一定程度上有赖于EE和PE的二级结构特征 . 展开更多
关键词 yeast基因 多聚腺苷作用信号 MRNA二级结构 蛋白质合成
下载PDF
Yeast基因组编码区特征参数的研究 被引量:2
18
作者 张颖 李宏 +1 位作者 吕军 罗辽复 《生物物理学报》 CAS CSCD 北大核心 2001年第3期535-541,共7页
以碱基成分偏移量D值[1]为基本参数定义参数d ,以d为Yeast编码区的特征参数 ,对Yeast的第1、2、3类ORF(openreadingframe)进行了统计 ,得到d的特征参数区间。并且 ,以此区间为标准对Yeast的6类ORF ,以及5′帽、3′尾、内含子、组分随机... 以碱基成分偏移量D值[1]为基本参数定义参数d ,以d为Yeast编码区的特征参数 ,对Yeast的第1、2、3类ORF(openreadingframe)进行了统计 ,得到d的特征参数区间。并且 ,以此区间为标准对Yeast的6类ORF ,以及5′帽、3′尾、内含子、组分随机序列等非编码序列进行了检验。结果表明 ,用d作编码区的特征参数是可行的 ,它可以很好地区分编码序列和非编码序列。另外 ,又讨论了参数d与基因表达水平 (用CAI值来衡量 )的关系。发现 ,参数d与基因表达水平成很好的正相关关系 ;发现密码子的第1位点和第2位点的某些碱基分布与基因表达水平有关。 展开更多
关键词 yeast基因组 编码区 碱基成分偏移 正相关 特征参数
下载PDF
Detection for Transcriptional Activity of Alternaria Tenuissim Protein Elicitor in Yeast Two-hybrid System 被引量:3
19
作者 刘延锋 邱德文 +1 位作者 曾洪梅 杨秀芬 《Agricultural Science & Technology》 CAS 2008年第1期64-66,共3页
The peaT1 gene fragment was amplified from pGEM-6p-l-peaT1 by PCR, and recovered target gene was cloned into pLexA vector. After digestion and sequencing, the bait vector pLexA-peaT1 was transformed into yeast strain ... The peaT1 gene fragment was amplified from pGEM-6p-l-peaT1 by PCR, and recovered target gene was cloned into pLexA vector. After digestion and sequencing, the bait vector pLexA-peaT1 was transformed into yeast strain EGY48 [p8op-lacZ] by PEG/LiAC, and the transcriptional activity of bait vector was detected. The results showed that recombinant bait plasmid pLexA-PEMG1 was constructed, for the two bands of recombinant bait plasmid in agarose gel eleetrophoresis were expected after digesting by restriction endonuclease EcoR I and Xho I. Therefore, the recombinant bait plasmid could be used in yeast two-hybrid system to screen a cDNA library. 展开更多
关键词 PeaT1 yeast two-hybrid Transcriptional activity
下载PDF
The Self-activated Experimental of T_(1083) Substitution Mutation Vector pGBKT7-TS in Yeast 被引量:3
20
作者 袁亮 纪耀坤 张伟彬 《Agricultural Science & Technology》 CAS 2010年第3期65-67,共3页
[Objective] The research aimed to study the self-activation test of inverting T1083 substitution mutation BD fusion vector pGBKT7-TS into yeast,and discuss whether its expression product can be used as bait for furthe... [Objective] The research aimed to study the self-activation test of inverting T1083 substitution mutation BD fusion vector pGBKT7-TS into yeast,and discuss whether its expression product can be used as bait for further two-hybrid screening.[Method] T1083 substitution mutation BD fusion vector pGBKT7-TS was inverted into yeast to make the self-activation and protein expression toxic detection test.[Result] This expression product of the fragment was inactive and had no toxic to yeast cell.It could be used as bait for further two-hybrid screening.[Conclusion] The research laid the experimental foundation for further study of the effect of T1083 substitution mutation on the interaction of NtKrp and its target partner. 展开更多
关键词 pGBKT7-TS vector yeast two-hybrid Bait vector SELF-ACTIVATION
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部