Calcium-chelating peptides from rabbit bone collagen:characterization,identification and mechanism elucidation

This study aimed to characterize and identify calcium-chelating peptides from rabbit bone collagen and explore the underlying chelating mechanism.Collagen peptides and calcium were extracted from rabbit bone by instant ejection steam explosion(ICSE)combined with enzymatic hydrolysis,followed by chelation reaction to prepare rabbit bone peptide-calcium chelate(RBCP-Ca).The chelating sites were further analyzed by liquid chromatography-tandem mass(LC-MS/MS)spectrometry while the chelating mechanism and binding modes were investigated.The structural characterization revealed that RBCP successfully chelated with calcium ions.Furthermore,LC-MS/MS analysis indicated that the binding sites included both acidic amino acids(Asp and Glu)and basic amino acids(Lys and Arg),Interestingly,three binding modes,namely Inter-Linking,Loop-Linking and Mono-Linking were for the first time found,while Inter-Linking mode accounted for the highest proportion(75.1%),suggesting that chelation of calcium ions frequently occurred between two peptides.Overall,this study provides a theoretical basis for the elucidation of chelation mechanism of calcium-chelating peptides.

Chelators to assist the high dispersion of Ni_(2)P particles over mesoporous silica nanospheres for hydrogenating reaction

NizP supported catalysts exhibit high catalytic activities in hydrogenation reaction,of which the particle sizes of Ni_(2)P active phases are the key influential factor.This research focus on the effect of chelators on the size of Ni_(2)P particles over wrinkle silica nanoparticles(WSNs)by introducing chelating agents EDTA and NTA during impregnation process.The characterization results show that chelators modified cata-lysts possess smaller size of Ni_(2)P particles than the unmodified Ni_(2)P catalysts.Among all the synthesized catalysts,the EDTA modified Ni_(2)PE(1.5)/WSNs catalyst possesses smallest average particle size of Ni_(2)P,only 2.6 nm.Moreover,the Ni_(2)P catalysts with the assistance of EDTA exhibits better catalytic activity than that of NTA under high reaction temperature,which can be ascribed to the strong bonding between EDTA and Ni.And the EDTA modified Ni_(2)PE(1.5)/WSNs catalyst shows highest hydrogenation ability,almost reaching 100%decalin selectivity.

Polarizable Additive with Intermediate Chelation Strength for Stable Aqueous Zinc‑Ion Batteries

Aqueous zinc-ion batteries are promising due to inherent safety,low cost,low toxicity,and high volumetric capacity.However,issues of dendrites and side reactions between zinc metal anode and the electrolyte need to be solved for extended storage and cycle life.Here,we proposed that an electrolyte additive with an intermediate chelation strength of zinc ion—strong enough to exclude water molecules from the zinc metal-electrolyte interface and not too strong to cause a significant energy barrier for zinc ion dissociation—can benefit the electrochemical stability by suppressing hydrogen evolution reaction,overpotential growth,and den-drite formation.Penta-sodium diethylene-triaminepentaacetic acid salt was selected for such a purpose.It has a suitable chelating ability in aqueous solutions to adjust solvation sheath and can be readily polarized under electrical loading conditions to further improve the passivation.Zn||Zn symmetric cells can be stably operated over 3500 h at 1 mA cm^(-2).Zn||NH4V4O10 full cells with the additive show great cycling stability with 84.6%capacity retention after 500 cycles at 1 A g^(-1).Since the additive not only reduces H2 evolution and corrosion but also modifies Zn2+diffusion and deposition,highlyreversible Zn electrodes can be achieved as verified by the experimental results.Our work offers a practical approach to the logical design of reliable electrolytes for high-performance aqueous batteries.

Role of copper chelating agents: between old applications and new perspectives in neuroscience

The role of copper element has been an increasingly relevant topic in recent years in the fields of human and animal health, for both the study of new drugs and innovative food and feed supplements. This metal plays an important role in the central nervous system, where it is associated with glutamatergic signaling, and it is widely involved in inflammatory processes. Thus, diseases involving copper(Ⅱ) dyshomeostasis often have neurological symptoms, as exemplified by Alzheimer's and other diseases(such as Parkinson's and Wilson's diseases). Moreover, imbalanced copper ion concentrations have also been associated with diabetes and certain types of cancer, including glioma. In this paper, we propose a comprehensive overview of recent results that show the importance of these metal ions in several pathologies, mainly Alzheimer's disease, through the lens of the development and use of copper chelators as research compounds and potential therapeutics if included in multi-target hybrid drugs. Seeing how copper homeostasis is important for the well-being of animals as well as humans, we shortly describe the state of the art regarding the effects of copper and its chelators in agriculture, livestock rearing, and aquaculture, as ingredients for the formulation of feed supplements as well as to prevent the effects of pollution on animal productions.

Electrode/Electrolyte Interfacial Chemistry Modulated by Chelating Effect for High-Performance Zinc Anode

Although Zn metal has been regarded as the most promising anode for aqueous batteries,its practical application is still restricted by side reactions and dendrite growth.Herein,an in-situ solid electrolyte interphase(SEI)film formed on the interface of electrode/electrolyte during the plating/stripping of zinc anodes by introducing trace amounts of multidentate ligand sodium diethyldithiocarbamate(DDTC)additive into 1 M ZnSO_(4).The synergistic effect of in-situ solid electrolyte interphase forming and chelate effect endows Zn^(2+)with uniform and rapid interface-diffusion kinetics against dendrite growth and surface side reactions.As a result,the Zn anode in 1 M ZnSO_(4)+DDTC electrolytes displays an ultra-high coulombic efficiency of 99.5%and cycling stability(more than 2000 h),especially at high current density(more than 600 cycles at 40 mA cm^(-2)).Moreover,the Zn//MnO_(2)full cells in the ZnSO_(4)+DDTC electrolyte exhibit outstanding cyclic stability(with 98.6%capacity retention after 2000 cycles at 10 C).This electrode/electrolyte interfacial chemistry modulated strategy provides new insight into enhancing zinc anode stability for high-performance aqueous zinc batteries.

Synthesis and Characteristics of A Novel Heavy Metal Ions Chelator

Polyacrylamide-urea-sulfanilamide（PUS） was prepared as a novel heavy metal ions chelator and successfully used to simultaneously remove heavy metals from wastewater effluents.The effects of reaction parameters（sodium hydroxide,material ratio,temprature and contact time） were monitored to specify the best synthesis conditions.PUS was chemically characterized by means of infrared spectroscopy（FTIR） and ultraviolet-visible（UV-Vis）.The simultaneous chelation performance of PUS towards selected heavy metals ions,Ni2＋,Cu2＋,Pb2＋,Zn2＋,Cd2＋ was discussed,showing that Ni2＋,Cu2＋,Pb2＋,Zn2＋ could be better chelated.It is indicated that the synthesized PUS is a potential remediation material when used for the treatment of wastewater containing metal ions.

Effects of an oral iron chelator, deferasirox, on advanced hepatocellular carcinoma

AIM To evaluate the inhibitory effects of deferasirox(DFX) against hepatocellular carcinoma(HCC) through basic and clinical studies.METHODS In the basic study, the effect of DFX was investigated in three hepatoma cell lines(Hep G2, Hep3 B, and Huh7), as well as in an N-nitrosodiethylamine-induced murine HCC model. In the clinical study, six advanced HCC patients refractory to chemotherapy were enrolled. The initial dose of DFX was 10 mg/kg per day and was increased by 10 mg/kg per day every week, until the maximum dose of 30 mg/kg per day. The duration of a single course of DFX therapy was 28 consecutive days. In the event of dose-limiting toxicity(according to the Common Terminology Criteria for Adverse Events v.4.0), DFX dose was reduced.RESULTS Administration of DFX inhibited the proliferation of hepatoma cell lines and induced the activation of caspase-3 in a dose-dependent manner in vitro. In the murine model, DFX treatment significantly suppressed the development of liver tumors(P < 0.01), and significantly upregulated the mR NA expression levels of hepcidin(P < 0.05), transferrin receptor 1(P < 0.05), and hypoxia inducible factor-1α(P < 0.05) in both tumor and non-tumor tissues, compared with control mice. In the clinical study, anorexia and elevated serum creatinine were observed in four and all six patients, respectively. However, reduction in DFX dose led to decrease in serum creatinine levels in all patients. After the first course of DFX, one patient discontinued the therapy. We assessed the tumor response in the remaining five patients; one patient exhibited stable disease, while four patients exhibited progressive disease. The one-year survival rate of the six patients was 17%.CONCLUSION We demonstrated that DFX inhibited HCC in the basic study, but not in the clinical study due to dose-limiting toxicities.

Evaluation of a novel oral iron chelator 1-(N-acetyl-6-aminohexyl)-3-hydroxypyridin-4-one (CM1) for treatment of iron overload in mice

Desferrioxamine (DFO), deferiprone (DFP) and deferasirox (DFX) are promising effective iron chelators for the treatment of iron overload in b-thalassemia patients;nonetheless, their side effects have also been reported. 3-Hydroxypyridinone derivatives are being developed as a safer new chelator and in combined chelation therapy. We evaluated the iron-chelating activity of 1-(N-acetyl-6-aminohexyl)-3-hydroxypyridin-4-one (CM1) in iron-loaded C57BL6 mice. The feeding of a ferrocene-supplemented diet (Fe diet) to mice resulted in iron overload, detectable plasma nontransferrin-bound iron (NTBI) and labile plasma iron (LPI), and increases of red cell membrane iron, plasma malondialdehyde (MDA) and excessive tissue iron deposits. Like DFP, the CM1 lowered the levels of the membrane non-heme iron, the NTBI and LPI (p < 0.05) and the MDA after 3 months of treatment. Administration of the Fe diet and the Fe diet along with the chelators did not change the morphology of the liver and heart. Numerous iron accumulations were observed in the liver and spleen tissues of the Fe dietfed mice, whereas the CM1 reduced such iron deposition. Thus, 1-(N-acetyl-6-aminohexyl)-3-hydro- xypyridin-4-one (CM1) can be considered a candidate bidentate oral iron chelator and is effective in the removal of toxic irons in blood compartment and tissues. The effectiveness and toxicity of the CM1 need to be investigated extensively in thalassemia mice and patients with iron overload.

Remediation of Heavy Metal (Cd, Cr, Cu, Co, and Ni) Ions from Kaolinite Clay Using Molecular Micelles Chelators and D-Optimum Experimental Design

This study investigated the potential utility of poly (sodium N-undecanoyl-L-leucyl-valinate) (poly-L-SULV), poly (sodium N-undecanoyl-L-leucyl-alanate) (poly-L-SULA), and poly (sodium N-undecanoyl-glycinate) (poly-SUG) molecular micelles (MMs) as chelators for heavy metal (Cd, Cr, Cu, Co, and Ni) ion remediation of kaolinite clay using D-optimum experimental design. D-optimum experimental design was employed to simultaneously investigate the influence of design variables such as the buffer pH, chelator concentration, and centrifuge speed to evaluate the optimum conditions and to reduce the time and cost of metal ion remediation. The partition coefficients of the metal ion concentrations between the kaolinite clay and chelator equilibrium were also evaluated. In addition, the influence of metal ion concentrations on the remediation capability of the chelators was evaluated by conducting remediation studies at four different (10 ppm, 40 ppm, 60 ppm, and 80 ppm) metal ion concentrations. In general, the results of the remediation efficiency and partition coefficients obtained in this study are highly metal ion dependent and also dependent upon the chelator used for the remediation. Specifically, the remediation efficiency of the molecular micelles was found to be comparable to or better than the corresponding remediation efficiency obtained when SDS or EDTA was used for the remediation. However, at optimum conditions, poly-SULV and poly-L-SULA molecular micelle chelators demonstrated superior remediation efficiencies for Cr, with remediation efficiency of 99.9 ± 8.7% and 99.1 ± 0.7%, respectively.

Natural Iron Chelators: An Orthomolecular Approach to Treat Iron Overload and Its Related Diseases

Despite its various vital roles in the different body’s metabolisms,iron may have a hazardous impact on health when it exceeds its normal values.Iron overload is triggered by many genetic and behavioral factors.Furthermore,excessive iron levels have also been observed in many pathologies such as Alzheimer’s,Parkinson’s,cardiovascular and some cancerous diseases.This paper describes a set of natural iron chelators as an effective and a safe orthomolecular approach in chelating iron.Orthomolecular medicine is based on providing patients with nutritional supplementation at high doses to treat and prevent diseases.This paper describes the properties of a set of flavonoids and phenolic acids such as curcumin and ferulic acid that can be administered as supplements to patients suffering from iron overload since they are classified as strong chelators.Those natural iron chelators’supplements are mainly extracted from fruits,vegetables,and plants.As chelators,they are able to bind effectively to iron,inhibit the production of reactive oxygen species,and reduce the levels of oxidative stress.They can also play an effective therapeutic role in the treatment of neurodegenerative,cardiovascular,diabetic,and cancerous diseases thanks to their iron chelation,antioxidant,and anti-inflammatory properties.

Tolerance to Zn deficiency and P-Zn interaction in wheat seedlings cultured in chelator-buffered solutions

Zinc deficiency is a common constraint for wheat production in the regions with limited precipitation,particularly in the regions with high levels of available phosphate （P） in soil.Two experiments were conducted using chelator-buffered nutrient solutions to characterize differences in tolerance to Zn deficiency among three winter wheat （Triticum aestivum L）.genotypes and to investigate the relationship between P and Zn nutrition in wheat species.Four indices,Zn efficiency,relative shoot-to-root ratio,total Zn uptake in shoot,and shoot dry weight were used to compare the tolerance to Zn deficiency among three wheat genotypes.The results indicated that the four indices could be used in breeding selection for Zn uptake-efficient genotypes.The genotype H6712 was the most tolerant to Zn deficient,followed by M19,and then X13.Specifically,H6712 had the highest Zn uptake efficiency among the three genotypes.The addition of P to the growth medium increased Zn uptake and translocation from roots to shoots.Total Zn content of the wheat plant was 43% higher with 0.6 mmol/L P treatment than that of control with 0 mmol /L P treatment.The Zn translocation ratios from roots to shoots were increased by 16% and 26% with 0.6 mmol/L P treatment and 3 mmol/L P treatment,respectively,compared with 0 mmol/L P treatment.In contrast,high Zn concentrations in the growth medium inhibited P translocation from roots to shoots,but the inhibitive effects were not strong.Sixty-six percent of P taken up by wheat plants was translocated to the wheat shoots at 0 μmol/L Zn treatment,while the percent was 60% at 3 μmol/L Zn treatment.The result may be due to the fact that the wheat plants need more P than Zn.

Investigation of the effect of diethylene triamine pentaacetic acid chelating agent as an enhanced oil recovery fluid on wettability alteration of sandstone rocks

This study used the diethylene triamine pentaacetic acid(DTPA)-seawater(SW)system to modify the sandstone rock wettability and enhance oil recovery.The investigation involved conducting wettability measurement,Zeta potential measurement,and spontaneous imbibition experiment.The introduction of 5%DTPA-sW solution resulted in a significant decrease in the rock-oil contact angle from 143°to 23,along with a reduction in the Zeta potential from-2.29 mV to-13.06 mV,thereby altering the rock surface charge and shifting its wettability from an oil-wet state to a strongly water-wet state.The presence or absence of potential determining ions(Ca^(2+),Mg^(2+),SO_(4)^(2-))in the solution did not impact the effectiveness of DTPA in changing the rock wettability.However,by tripling the concentration of these ions in the solution,the performance of 5%DTPA-SW solution in changing wettability was impaired.Additionally,spontaneous imbibition tests demonstrated that the 5%DTPA-SW solution led to an increase in oil recovery up to 39.6%.Thus,the optimum mass fraction of DTPA for changing sandstone wettability was determined to be5%.

重金属捕获剂EDTC协同处理EDTA-Cu/RhB的去除特性

以乙二胺(C_(2)H_(8)N_(2))和二硫化碳(CS_(2))为原料,采用共混法合成重金属捕集剂N,N-双(二硫代羧基)乙二胺(EDTC)。考察物料配比、投加顺序、溶液pH、时间和温度等因素对EDTC作为药剂协同处理废水中络合态铜(EDTA-Cu)和染料罗丹明B(Rhodamine B,RhB)双组分体系的去除特性影响,并与单组分EDTA-Cu和RhB体系的去除效果进行对比。结果表明:常温下,于180mg/L的EDTA-Cu和1800mg/L的RhB水体中投加1.3g/L EDTC时,EDTA-Cu和RhB的去除量分别高达150mg/g和850mg/g,水体pH在2~10范围内运行稳定。与EDTC处理水体中单组分EDTA-Cu和RhB的去除效果相比,EDTC可有效同时去除水体中的络合铜和RhB,且具有协同效应。因此,EDTC可作为水处理药剂应用于协同处理带有络合态金属和染料的复杂废水。

类乙二胺四乙酸螯合纤维素及其对水中重金属离子的吸附性能

为了改善纤维素对重金属的吸附去除性能,文中采用高碘酸钠选择性氧化纤维制得双醛纤维素,通过Schiff碱反应,将乙二胺接枝到纤维素骨架,再将溴乙酸取代到氨基上,获得含有氮和氧配位原子的类乙二胺四乙酸螯合纤维素(EDTA-CL)。表征了其形貌和结构,考察了其对水中Cd^(2+)和Pb^(2+)的吸附性能。EDTA-CL吸附Cd^(2+)和Pb^(2+)的最佳pH范围为4.5~6;EDTA-CL对Cd^(2+)和Pb^(2+)的吸附在20 min内达到了平衡;EDTA-CL对Cd^(2+)和Pb^(2+)的吸附容量分别为236.1 mg/g和382.6 mg/g,氮和氧配位原子的引入大大提高了对Cd^(2+)和Pb^(2+)的吸附容量;EDTA-CL对Cd^(2+)和Pb^(2+)的吸附过程符合Langmuir和准二级动力学模型;EDTA-CL可以重复多次使用。

玉米肽螯合钙纳米粒的制备及结构表征分析

为了探明玉米肽与钙离子螯合纳米粒的结构与特性,以小分子玉米肽(CPT)为钙离子螯合载体,采用有机溶剂沉淀法制备玉米肽纳米螯合钙(CPT-Ca)复合纳米粒,研究了其制备工艺、结构表征及体外抗氧化活性。结果表明,玉米肽螯合钙(CPT-Ca)的最佳制备条件为:玉米肽与氯化钙质量比40∶1,pH 7.0,螯合时间为60 min,温度为50℃,此条件下肽钙螯合率为(81.20±3.55)%。该螯合钙粒子的平均粒径为(396.0±8.0)nm,多分散系数为0.043,Zeta电位为(-22.6±0.7)mV,具有良好的纳米粒子微观结构特性;傅里叶红外光谱(FTIR)、紫外吸收光谱(UV)和X射线衍射图谱(XRD)显示,玉米肽上的氨基与羧基提供结合位点以配位键方式与钙离子结合,形成新的肽-钙螯合物;抗氧化实验表明,螯合钙具有较好的羟基和DPPH自由基清除自由基能力。玉米肽螯合钙纳米粒对于改善钙离子的结构分布和体内外传递吸收,开发新型玉米肽功能食品与钙离子补充剂具有重要作用。

铁死亡对阿尔茨海默病的影响及药物研究进展

阿尔茨海默病(AD)是一种慢性中枢神经系统退行性疾病。铁死亡是近年来发现的新型铁依赖性细胞死亡形式,典型特征为铁积累过多、谷胱甘肽过氧化物酶4失活、活性氧和脂质过氧化物升高。近年来的证据表明,铁死亡在AD的病理过程中起重要作用,但其具体机制尚不清楚。本文综述了铁平衡失调导致老年斑和神经元纤维缠结、加剧神经炎症以及介导神经细胞死亡等潜在相互作用,并总结了铁螯合剂、抗氧化剂以及铁死亡调节因子等针对铁死亡相关的AD治疗策略,以期为AD诊断和治疗提供有价值的信息。

水杨酸螯合树脂对铀的吸附性能

利用低温氮气吸附—脱附法测定水杨酸螯合树脂的比表面积、孔径;考察了水杨酸螯合树脂的热力学稳定性;研究了溶液pH、ρ(Cl^(-))对水杨酸螯合树脂铀吸附性能的影响,以及树脂的吸附等温线、吸附动力学及解吸性能。结果表明:水杨酸螯合树脂的比表面积为32.34 m^(2)/g,孔径为32.08 nm,当温度低于180℃时,水杨酸螯合树脂具有良好的热稳定性;在pH为7~9时,树脂吸附性能较好,铀吸附量达14.1 mg/g(以干树脂计,下同);Cl^(-)的存在会降低树脂对铀的吸附量,当ρ(Cl^(-))为3.0 g/L时,铀吸附量降低约9.9%;当铀溶液的平衡浓度为400 mg/L时,树脂的铀吸附量达到最大,为153 mg/g;吸附20 h,吸附过程达到平衡。酸性解吸剂与碱性解吸剂对树脂均具有良好的解吸性能,解吸率均在94%以上。在ρ(U)=21.2 mg/L、ρ(Cl^(-))=3.36 g/L的真实铀浸出液中吸附24 h,水杨酸螯合树脂的铀吸附量达14.7 mg/g,具有较好的铀吸附性能。

氨基酸微量元素螯合物对草鱼肝脏和肠道健康的影响

为研究饲料中氨基酸微量元素螯合物(铁、锰、铜、锌)对草鱼(Ctenopharyngodon idellus)肝脏和肠道健康的影响。共设置6组等氮(30%)、等脂(4.7%)实验饲料,对照组(CON)为不添加铁、锰、铜、锌4种微量元素的基础饲料,在对照组配方基础上添加无机铁(50 mg/kg)、铜(2 mg/kg)、锰(10 mg/kg)、锌(40 mg/kg)制成IT100组,用氨基酸微量元素螯合物以25%(OT25组)、50%(OT50组)、75%(OT75组)和100%(OT100组)的比例替代IT100组中无机微量元素,投喂初始体质量(40.05±0.05) g的草鱼8周。研究显示,与对照组相比,饲料中添加无机微量元素和氨基酸微量元素螯合物显著提高了草鱼肝脏的谷胱甘肽过氧化物酶(GPx)、微量还原型谷胱甘肽(GSH)和总超氧化物歧化酶(SOD)活性,降低了丙二醛含量(P<0.05)。OT100组显著提高了过氧化氢酶(CAT)活性,且OT75组和OT100组GPx活性和GSH含量显著高于IT100组(P<0.05)。与对照组相比,各组草鱼的肝细胞形态和肝血窦丰度趋于正常。与对照组相比,除OT25组外,其余各组的肠道绒毛高度和杯状细胞数量显著提高,且OT75和OT100组显著高于IT100组。无机微量元素和氨基酸微量元素螯合物降低了草鱼肠道细胞因子白介素-6(il-6)和白介素-1β(il-1β)基因表达量,提高了肠道紧密连接蛋白闭锁小带蛋白-2(zo-2)和claudin12基因表达量。OT75组显著提高了zo-1和occludin基因表达量,且OT75组il-1β基因表达量显著低于IT100组。综上所述,无机微量元素和氨基酸微量元素螯合物均可显著改善草鱼肠道和肝脏健康,75%氨基酸微量元素螯合物的作用效果显著优于无机微量元素。

硫酸钡锶的结垢机理与化学除垢技术的研究现状

结垢会给油田生产带来严重危害,严重威胁生产流程的进行,降低生产流量,导致生产损失。注入的水、原生水与岩石之间存在化学作用,导致储层流体的组成复杂,因此难以控制无机结垢地层。碳酸盐(钙)、硫化物(铁、锌)和硫酸盐(钙、钡、锶)垢在油田中更为常见,结垢的形成取决于各种因素,包括但不限于温度、压力、溶液饱和度、流体动力学行为等。本文介绍了油气生产中常见的无机结垢类型、形成机理、热力学溶积度、结垢预测,以及化学除垢剂的研究现状与发展趋势,以期为油田硫酸钡锶垢的预防与除垢提供参考。

牛血红蛋白肽的酶解工艺优化及其亚铁螯合物结构、稳定性研究

本研究以牛血为原料,提取牛血红蛋白进行酶解,筛选最适的酶解蛋白酶,采用单因素结合响应面试验探究最佳酶解工艺,通过扫描紫外光谱,傅里叶红外光谱,扫描电镜和全自动氨基酸分析仪等技术对制备的牛血红蛋白肽铁螯合物(Bovine Hemoglobin Peptide Iron Chelate,BHP-Fe)进行结构表征,并通过热重分析和体外模拟胃肠道消化探究其体外稳定性。结果表明:胃蛋白酶和碱性蛋白酶分步酶解为最适酶解方法。在胃蛋白酶初步酶解的基础上,得到碱性蛋白酶最佳酶解条件:料液比1:3,酶解pH9.8,酶解温度41℃,酶添加量5900 U/g,酶解时间2 h。在此条件下,酶解得到牛血红蛋白肽(Bovine Hemoglobin Peptide,BHP)的Fe^(2+)螯合能力达72.11%,牛血红蛋白水解度达35.07%。紫外光谱和傅里叶红外光谱结果显示,Fe^(2+)与BHP肽链上的羧基氧和氨基氮发生螯合反应生成了不同于多肽的新物质;扫描电镜结果显示,多肽与Fe^(2+)螯合后从表面光滑的碎片状变成表面粗糙的块状,两者存在明显差异;氨基酸含量变化显示天冬氨酸、谷氨酸、赖氨酸为多肽与金属离子的结合提供结合位点;热重分析结果表明,BHP与BHP-Fe在300℃高温状态下依然能保持较好的稳定性;体外模拟消化稳定性分析结果表明,BHP-Fe相较于其它铁补充剂在胃肠道中能保持较高的稳定性。本实验制备的多肽源性补铁剂螯合力强、消化稳定性好、具有广阔应用前景,可为牛血副产物的高效利用和新型多肽源补铁剂的未来发展前景提供建设性参考。