Fruit cracking occurs easily during the late period of fruit development when plants encounter an unsuitable environment,dramatically affecting fruit production and marketing.This study conducted the bulked segregant ...Fruit cracking occurs easily during the late period of fruit development when plants encounter an unsuitable environment,dramatically affecting fruit production and marketing.This study conducted the bulked segregant RNA-Seq(BSR)to identify the key regulatory gene of fruit cracking in tomatoes.BSR-Seq analysis illustrated that two regions associated with irregularly cracking were located on chromosomes 9 and 11,containing 127 candidate genes.Further,through differentially expression analysis and qRT-PCR in cracking-susceptible and cracking-resistant genotypes,the candidate gene SlGH9-15(Solyc09g010210)with significantly differential expression levels was screened.Bioinformatics analysis of the GH9 gene family revealed that 20 SlGH9 genes were divided into three groups.The phylogenetic analysis showed that SlGH9-15 was closely related to cell wall construction-associated genes AtGH9B1,AtGH9B6,OsGH9B1,and OsGH9B3.The cis-acting elements analysis revealed that Sl GH9-15 was activated by various hormones(ethylene and ABA)and abiotic stresses.The expression pattern indicated that 13 SlGH9 genes,especially SlGH9-15,were highly expressed in the cracking-susceptible genotype.Its expression level gradually increased during fruit development and achieved maximum value at the red ripe stage.Additionally,the cracking-susceptible tomato showed higher cellulase activity and lower cellulose content than the cracking-resistant tomato,particularly at the red ripe stage.This study identified SlGH9-15 as a key gene associated with fruit cracking in tomatoes for the first time and gives new insights for understanding the molecular mechanism and complex regulatory network of fruit cracking.展开更多
基金supported by the National Key Research and Development Program of China(2019YFD100190200)the Jiangsu Agricultural Science and Technology Innovation Fund,China(CX(20)3101)a grant from the Fundamental Research Funds for the Central Universities,China(KYZZ2022004)。
文摘Fruit cracking occurs easily during the late period of fruit development when plants encounter an unsuitable environment,dramatically affecting fruit production and marketing.This study conducted the bulked segregant RNA-Seq(BSR)to identify the key regulatory gene of fruit cracking in tomatoes.BSR-Seq analysis illustrated that two regions associated with irregularly cracking were located on chromosomes 9 and 11,containing 127 candidate genes.Further,through differentially expression analysis and qRT-PCR in cracking-susceptible and cracking-resistant genotypes,the candidate gene SlGH9-15(Solyc09g010210)with significantly differential expression levels was screened.Bioinformatics analysis of the GH9 gene family revealed that 20 SlGH9 genes were divided into three groups.The phylogenetic analysis showed that SlGH9-15 was closely related to cell wall construction-associated genes AtGH9B1,AtGH9B6,OsGH9B1,and OsGH9B3.The cis-acting elements analysis revealed that Sl GH9-15 was activated by various hormones(ethylene and ABA)and abiotic stresses.The expression pattern indicated that 13 SlGH9 genes,especially SlGH9-15,were highly expressed in the cracking-susceptible genotype.Its expression level gradually increased during fruit development and achieved maximum value at the red ripe stage.Additionally,the cracking-susceptible tomato showed higher cellulase activity and lower cellulose content than the cracking-resistant tomato,particularly at the red ripe stage.This study identified SlGH9-15 as a key gene associated with fruit cracking in tomatoes for the first time and gives new insights for understanding the molecular mechanism and complex regulatory network of fruit cracking.
文摘为探讨路基冻胀变形对轨道不平顺及结构受力的影响规律,基于ANSYS有限单元分析方法,以哈大高速铁路冻胀区路基段为研究基础,建立了考虑限位凸台、凝胶树脂及层间粘结接触特征的CRTS Ⅰ型板式无砟轨道-路基冻胀冻融耦合精细化有限元模型.在此基础上,探讨局部冻胀区路基冻胀变形发生位置、不同冻胀波长及幅值对无砟轨道结构的影响,分析了短波冻胀下轨道不平顺、层间离缝特性与静力学性能.结果表明:短波冻胀时无砟轨道结构不平顺范围、变形、离缝、受力等各项指标均随冻胀波长的减小、冻胀峰值的增加而增大;冻胀发生于底座板板中时对轨道结构受力影响最大,冻胀发生于底座板伸缩缝时对轨道结构变形离缝影响最大;相对于轨道板结构,底座板承受拉应力最大,冻胀发生于底座板板中时结构受力影响更大;在轨道结构抗拉强度方面,底座板为限制结构,建议冻胀检修限值的波长为10 m、峰值为5 mm.
