[Objective] The present study aimed to establish a rapid method for the isolation of small amount DNA from citrus.[Method] By using the improved CTBA method,the genomic DNA was extracted respectively from 20,10,5 and ...[Objective] The present study aimed to establish a rapid method for the isolation of small amount DNA from citrus.[Method] By using the improved CTBA method,the genomic DNA was extracted respectively from 20,10,5 and 2.5 mg hybrid embryos of citrus,and then the DNA quality was detected and followed by SSR verification.[Result] The method was very simple and rapid,which needed less materials.In addition,the isolated DNA showed good purity with the OD260/OD280 of 1.8-2.1,and could meet the requirement for PCR-based technology,such as SSR,etc..[Conclusion] The method could be used for rapid extraction of small amount of genomic DNA from citrus.展开更多
基金Supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2007BAD47B07-02)Special Foundation for Science and Technology of Chongqing(CSTC,2007AB1040)~~
文摘[Objective] The present study aimed to establish a rapid method for the isolation of small amount DNA from citrus.[Method] By using the improved CTBA method,the genomic DNA was extracted respectively from 20,10,5 and 2.5 mg hybrid embryos of citrus,and then the DNA quality was detected and followed by SSR verification.[Result] The method was very simple and rapid,which needed less materials.In addition,the isolated DNA showed good purity with the OD260/OD280 of 1.8-2.1,and could meet the requirement for PCR-based technology,such as SSR,etc..[Conclusion] The method could be used for rapid extraction of small amount of genomic DNA from citrus.
