Isoperoxidases of fourteen species of poplars in Heilongjiang Province were analyzed by polyacrylamidegel disc-electrophoresis. The major zymogram of petioles of poplars was consisted of twelve kinds of isoenzymes, in...Isoperoxidases of fourteen species of poplars in Heilongjiang Province were analyzed by polyacrylamidegel disc-electrophoresis. The major zymogram of petioles of poplars was consisted of twelve kinds of isoenzymes, in which 35% zymogram was just the same and represented the specialized zymograms of poplars. The resuits of the experiments showed that the closer the affinity relationships of the species were, the greater the similar values between the zymograms were. There were very great similar values among natural hybrid species.But in sect. Tacamahaca Spach, the similar values between Populus koreana Rehder and P nakaii Skv were obviously smaller .Isoenzyme analysis provided new information for plant classification on the molecular level.展开更多
Objective:To explore the identity and phylogenetic relationships among the three medicinally important species of Sphaerostephanos from South India using isozymic profile.Methods:The young fronds were homogenized with...Objective:To explore the identity and phylogenetic relationships among the three medicinally important species of Sphaerostephanos from South India using isozymic profile.Methods:The young fronds were homogenized with 3.5 mL of ice-cold homogenizing buffer in a pre-chilled pestle and mortar.The supernatant was subjected to electrophoresis as described by Anbalagan poly acrylamide gel electrophoresis.Staining solutions for isoperoxidase was prepared as per Smila method for the detection of isoenzymes.Results:A total of six different bands in five different positions with different molecular weight/Rf values and four active zones have been observed in the isoperoxidase enzyme system of Sphaerostephanos.Only one band with MW/Rf 0.399 is common to two different species i.e.Sphaerostephanos arbuscula(S.arbuscula) and Sphaerostephanos unitus(S.unitus).Among the remaining four bands,two bands(R_f.0.23,0.47)are present in Sphaerostephanos subtruncatus(S.subtruncatus) and one distinct band has been observed individually in S.arbuscula(R_f.0.507) and S.unitus(R_f.0.56).Conclusions:The present preliminary molecular study through isozymic analysis shows the identity of all the three species and the present results confirm distinctness of these three species based on macromicromorphology,phytochemistry and cytology.展开更多
文摘Isoperoxidases of fourteen species of poplars in Heilongjiang Province were analyzed by polyacrylamidegel disc-electrophoresis. The major zymogram of petioles of poplars was consisted of twelve kinds of isoenzymes, in which 35% zymogram was just the same and represented the specialized zymograms of poplars. The resuits of the experiments showed that the closer the affinity relationships of the species were, the greater the similar values between the zymograms were. There were very great similar values among natural hybrid species.But in sect. Tacamahaca Spach, the similar values between Populus koreana Rehder and P nakaii Skv were obviously smaller .Isoenzyme analysis provided new information for plant classification on the molecular level.
文摘Objective:To explore the identity and phylogenetic relationships among the three medicinally important species of Sphaerostephanos from South India using isozymic profile.Methods:The young fronds were homogenized with 3.5 mL of ice-cold homogenizing buffer in a pre-chilled pestle and mortar.The supernatant was subjected to electrophoresis as described by Anbalagan poly acrylamide gel electrophoresis.Staining solutions for isoperoxidase was prepared as per Smila method for the detection of isoenzymes.Results:A total of six different bands in five different positions with different molecular weight/Rf values and four active zones have been observed in the isoperoxidase enzyme system of Sphaerostephanos.Only one band with MW/Rf 0.399 is common to two different species i.e.Sphaerostephanos arbuscula(S.arbuscula) and Sphaerostephanos unitus(S.unitus).Among the remaining four bands,two bands(R_f.0.23,0.47)are present in Sphaerostephanos subtruncatus(S.subtruncatus) and one distinct band has been observed individually in S.arbuscula(R_f.0.507) and S.unitus(R_f.0.56).Conclusions:The present preliminary molecular study through isozymic analysis shows the identity of all the three species and the present results confirm distinctness of these three species based on macromicromorphology,phytochemistry and cytology.
