A reverse-transcription loop-mediated isothermal amplification （RT-LAMP） method was established for the detection of wheat streak mosaic virus （WSMV）. Ac-cording to the conservative regions of the genes that encod...A reverse-transcription loop-mediated isothermal amplification （RT-LAMP） method was established for the detection of wheat streak mosaic virus （WSMV）. Ac-cording to the conservative regions of the genes that encode the coat protein of WSMV, 2 pairs of primers were designed. Final y, the 1st pair of primers was select-ed through the specificity test. The sensitivity test showed the sensitivity of RT-LAMP method was 10 times higher than that of RT-PCR. In addition, the amplifica-tion of target gene could be judged visual y from the presence of fluorescence （cal-cein） in the final reaction system. The RT-LAMP method, established in this study, was rapid, easy, specific and sensitive. Moreover, it did not require sophisticated equip-ment. The RT-LAMP was suitable for the rapid detection of WSMV.展开更多
[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification （LAMP）, improve the detection rate of canine parvovirus （CPV） and reduce the testing cost. [ Method] Acco...[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification （LAMP）, improve the detection rate of canine parvovirus （CPV） and reduce the testing cost. [ Method] According to the conserved regions of the VP2 gene of CPV, six primers were designed to amplify the special DNA sequences by LAMP. In addition, the reaction conditions of LAMP were optimized, and the sensitivity, specificity, repeatability and stability were verified. [ Result] The optimal reaction time of the LAMP method for CPVwas 60 min. The products obtained by LAMP had high specificity without cross-reaction with other generic viruses. The sensitivity of the LAMP was 100 times higher than that of PCR. [ Conclusion] The LAMP method for detecting CPV has high practical value. It has many advantages such as high specificity, high sensitivity, simple operation, low cost and rapid analysis, and it does not require special equipment. Therefore, this method is more suitable for the detection of CPV.展开更多
The flow behavior of Rene 95 PM alloy was studied from 1050 to 1150 deg Cwith strain rate of 1 X 10^(-3), 1 X 10^(-2), 1 X 10^(-1) and 1 s^(-1). At a given temperature andstrain rate, flow curves exhibit a peak follow...The flow behavior of Rene 95 PM alloy was studied from 1050 to 1150 deg Cwith strain rate of 1 X 10^(-3), 1 X 10^(-2), 1 X 10^(-1) and 1 s^(-1). At a given temperature andstrain rate, flow curves exhibit a peak followed by flow softening up to a steady state. Moreover,at constant strain, flow stress increases with increasing strain rate and decreasing temperature. Anequation relating hyperbolic sine of flow stress to hot working parameters, such as strain, strainrate and temperature, was established by using multiple nonlinear regression method. A very goodagreement was found between predicted and experimental flow stress in all the strain rangeinvestigated. Application of the constitutive equation in predicting forming loads and flow behaviorand temperature distribution in both upper and lower dies in an isothermal forging process ofturbine disk of large dimension (about 630 mm) by means of a finite element code was systematicallyanalyzed.展开更多
The thermal decomposition processes of ephedrini hydrochloridum and its kinetics are studied by TG-DTG techniques. A combined method, which includes Achar method, Coats-Redfera method, and Ozawa method, is put forward...The thermal decomposition processes of ephedrini hydrochloridum and its kinetics are studied by TG-DTG techniques. A combined method, which includes Achar method, Coats-Redfera method, and Ozawa method, is put forward for determining kinetic model under non-isothermal conditions. By applying the combined method, it is determined that the thermal decomposition of ephedrini hydrochloridum is subjected to cylindrical symmetric diffusion. And the reaction function isƒ(α)=2(1-α)?, apparent activation energy (115.26±3.55) kJ·mol−1, pre-exponential factor 4.62×108 s−1. Results show that the combined method is feasible and simple.展开更多
In this article an investigation is presented on the properties of dust acoustic（DA）compressive solitary wave propagation in an adiabatic dusty plasma,including the effect of nonthermal positive and negative ions an...In this article an investigation is presented on the properties of dust acoustic（DA）compressive solitary wave propagation in an adiabatic dusty plasma,including the effect of nonthermal positive and negative ions and non-isothermal electrons.The reductive perturbation method has been employed to derive the lower degree modified Kadomtsev-Petviashivili（mK-P）,3D Schamel-Korteweg-de-Vries equation or modified Kadomtsev-Petviashivili（mK-P） equations for dust acoustic solitary waves in a homogeneous,unmagnetized and collisionless plasma whose constituents are non-isothermal electrons,singly charged positive and negative non-thermal ions and massive charged dust particles.The stationary analytical solutions of the lower degree mK-P and mK-P equations are numerically analyzed,where the effect of various dusty plasma constituents on DA solitary wave propagation is taken into account.It is observed that both the ions in dusty plasma play a key role in the formation of DA compressive solitary waves,and also the ion concentration and non-isothermal electrons control the transformation of the compressive potentials of the waves.展开更多
The investigation of adsorption and desorption properties of shale are important for estimating reserves and exploitation. The shale samples used in this paper were from the marine shale on Longmaxi shale in Sichuan a...The investigation of adsorption and desorption properties of shale are important for estimating reserves and exploitation. The shale samples used in this paper were from the marine shale on Longmaxi shale in Sichuan and Hubei province, China. A series of analyses, such as organic carbon content test, vitrinite reflectance test, rock pyrolysis, X-ray diffraction, and N2/CO2 adsorption were performed. Gravimetric method with magnetic suspension balance was used to conduct isothermal adsorption and desorption experiments. The Langmuir, Freundlich, Langmuir-Freundlich, D-R, semi-pore, and Tothequations were used to fit the isothermal adsorption and desorption curves. And adsorption potential theory was used to explain the adsorption and desorption process. According to the results, the shale samples have a high level of organic carbon content with the same organic matter type II1 and high degree of maturation. The volume of adsorption increases rapidly and slows down to stable with the pressure increasing. Desorption is the inverse process of adsorption and 10 MPa - 0.5 MPa is the main period of shale gas desorption. The fitting results show that three-parameter isotherm equations are better than the two-parameter ones. The adsorption temperature has a great influence on adsorption volume, little effect on potential energy. Adsorption potential varies under different TOC to affect adsorption properties. Moreover, a large adsorption potential means that the gas molecule is easy to adsorb but difficult to desorb.展开更多
文摘A reverse-transcription loop-mediated isothermal amplification （RT-LAMP） method was established for the detection of wheat streak mosaic virus （WSMV）. Ac-cording to the conservative regions of the genes that encode the coat protein of WSMV, 2 pairs of primers were designed. Final y, the 1st pair of primers was select-ed through the specificity test. The sensitivity test showed the sensitivity of RT-LAMP method was 10 times higher than that of RT-PCR. In addition, the amplifica-tion of target gene could be judged visual y from the presence of fluorescence （cal-cein） in the final reaction system. The RT-LAMP method, established in this study, was rapid, easy, specific and sensitive. Moreover, it did not require sophisticated equip-ment. The RT-LAMP was suitable for the rapid detection of WSMV.
基金supported by Independent Innovation Specific Projects of Shandong Province ( 2008ZHZX1A1103)
文摘[Objective] To develop a rapid and visualized detection method using loop-mediated isothermal amplification （LAMP）, improve the detection rate of canine parvovirus （CPV） and reduce the testing cost. [ Method] According to the conserved regions of the VP2 gene of CPV, six primers were designed to amplify the special DNA sequences by LAMP. In addition, the reaction conditions of LAMP were optimized, and the sensitivity, specificity, repeatability and stability were verified. [ Result] The optimal reaction time of the LAMP method for CPVwas 60 min. The products obtained by LAMP had high specificity without cross-reaction with other generic viruses. The sensitivity of the LAMP was 100 times higher than that of PCR. [ Conclusion] The LAMP method for detecting CPV has high practical value. It has many advantages such as high specificity, high sensitivity, simple operation, low cost and rapid analysis, and it does not require special equipment. Therefore, this method is more suitable for the detection of CPV.
文摘The flow behavior of Rene 95 PM alloy was studied from 1050 to 1150 deg Cwith strain rate of 1 X 10^(-3), 1 X 10^(-2), 1 X 10^(-1) and 1 s^(-1). At a given temperature andstrain rate, flow curves exhibit a peak followed by flow softening up to a steady state. Moreover,at constant strain, flow stress increases with increasing strain rate and decreasing temperature. Anequation relating hyperbolic sine of flow stress to hot working parameters, such as strain, strainrate and temperature, was established by using multiple nonlinear regression method. A very goodagreement was found between predicted and experimental flow stress in all the strain rangeinvestigated. Application of the constitutive equation in predicting forming loads and flow behaviorand temperature distribution in both upper and lower dies in an isothermal forging process ofturbine disk of large dimension (about 630 mm) by means of a finite element code was systematicallyanalyzed.
基金the Foundation of the Science and Technology Committee of Hubei Province(2001ABA009)
文摘The thermal decomposition processes of ephedrini hydrochloridum and its kinetics are studied by TG-DTG techniques. A combined method, which includes Achar method, Coats-Redfera method, and Ozawa method, is put forward for determining kinetic model under non-isothermal conditions. By applying the combined method, it is determined that the thermal decomposition of ephedrini hydrochloridum is subjected to cylindrical symmetric diffusion. And the reaction function isƒ(α)=2(1-α)?, apparent activation energy (115.26±3.55) kJ·mol−1, pre-exponential factor 4.62×108 s−1. Results show that the combined method is feasible and simple.
文摘In this article an investigation is presented on the properties of dust acoustic（DA）compressive solitary wave propagation in an adiabatic dusty plasma,including the effect of nonthermal positive and negative ions and non-isothermal electrons.The reductive perturbation method has been employed to derive the lower degree modified Kadomtsev-Petviashivili（mK-P）,3D Schamel-Korteweg-de-Vries equation or modified Kadomtsev-Petviashivili（mK-P） equations for dust acoustic solitary waves in a homogeneous,unmagnetized and collisionless plasma whose constituents are non-isothermal electrons,singly charged positive and negative non-thermal ions and massive charged dust particles.The stationary analytical solutions of the lower degree mK-P and mK-P equations are numerically analyzed,where the effect of various dusty plasma constituents on DA solitary wave propagation is taken into account.It is observed that both the ions in dusty plasma play a key role in the formation of DA compressive solitary waves,and also the ion concentration and non-isothermal electrons control the transformation of the compressive potentials of the waves.
文摘The investigation of adsorption and desorption properties of shale are important for estimating reserves and exploitation. The shale samples used in this paper were from the marine shale on Longmaxi shale in Sichuan and Hubei province, China. A series of analyses, such as organic carbon content test, vitrinite reflectance test, rock pyrolysis, X-ray diffraction, and N2/CO2 adsorption were performed. Gravimetric method with magnetic suspension balance was used to conduct isothermal adsorption and desorption experiments. The Langmuir, Freundlich, Langmuir-Freundlich, D-R, semi-pore, and Tothequations were used to fit the isothermal adsorption and desorption curves. And adsorption potential theory was used to explain the adsorption and desorption process. According to the results, the shale samples have a high level of organic carbon content with the same organic matter type II1 and high degree of maturation. The volume of adsorption increases rapidly and slows down to stable with the pressure increasing. Desorption is the inverse process of adsorption and 10 MPa - 0.5 MPa is the main period of shale gas desorption. The fitting results show that three-parameter isotherm equations are better than the two-parameter ones. The adsorption temperature has a great influence on adsorption volume, little effect on potential energy. Adsorption potential varies under different TOC to affect adsorption properties. Moreover, a large adsorption potential means that the gas molecule is easy to adsorb but difficult to desorb.
