Objective To determine the immune status of T lymphocyte subsets of patients with chronic lymphedema of extremities and the effects of microwave treatment. Methods Patients with lymphedema of extremities (n = 20) and ...Objective To determine the immune status of T lymphocyte subsets of patients with chronic lymphedema of extremities and the effects of microwave treatment. Methods Patients with lymphedema of extremities (n = 20) and normal volunteers (n = 10 ) were studied by fiuorescein isothiocyanate (FITC) or phycoerythrin (PE)-conjugated monoclonal antibo dies (MoAbs) and dual color flow cytometry to examine the changes of lymphocyte phenotype in them before and after treatment. Results The percentage of CD+4 T lympcutes and CD+4 /CD+8 T cell ratios in lymphedema group were less than those in control group before treatment. They increased significantly and restored to nearly normal level after microwave treatment. The percentage of CD +8 and HLA-DR + T lymphocytes in lymphedema group also demonstrated significant decline after treat- ment. Conclusion Disorder of T lymphocyte subsets existed in patients with chronic lymphedema of extremities, and microwave treatment can improve the states and enhance the cellular immunity of the patients.展开更多
文摘Objective To determine the immune status of T lymphocyte subsets of patients with chronic lymphedema of extremities and the effects of microwave treatment. Methods Patients with lymphedema of extremities (n = 20) and normal volunteers (n = 10 ) were studied by fiuorescein isothiocyanate (FITC) or phycoerythrin (PE)-conjugated monoclonal antibo dies (MoAbs) and dual color flow cytometry to examine the changes of lymphocyte phenotype in them before and after treatment. Results The percentage of CD+4 T lympcutes and CD+4 /CD+8 T cell ratios in lymphedema group were less than those in control group before treatment. They increased significantly and restored to nearly normal level after microwave treatment. The percentage of CD +8 and HLA-DR + T lymphocytes in lymphedema group also demonstrated significant decline after treat- ment. Conclusion Disorder of T lymphocyte subsets existed in patients with chronic lymphedema of extremities, and microwave treatment can improve the states and enhance the cellular immunity of the patients.
