Optimization ratio between Coptis chinensis and cinnamo of Jiaotai pill plus benzodiazepines for insomnia in China:a network meta-analysis

OBJECTIVE To explore the efficacy of Jiaotai pill(JTW)and JTW modified prescriptions(JTW^(+))plus benzodiazepines(BDZ)for insomnia in China by systematic review and network meta-analysis,especially optimization ratio(_(m:n))between Coptis chinensis and Cinnamo.METHODS Randomized controlled trials(RCTs)regarding JTW and JTW^(+)+BDZ for insomina were comprehensively searched in CNKI,VIP,Wanfang,and PubMed,Cochrane Library,OVID,Embase databases from their inception to December 2017.The risk of bias was assessed by the Cochrane collaboration′s tool and Jadad scale.Systematic review and network meta-analysis were performed to evaluate the relative risk(RR)plus 95%confidence interval(95%CI)or prediction interval(95%PrI),and draw surface under the cumulative ranking curves(SUCRA)by Stata 14.0 and RevMan 5.0 software.RESULTS A total of 13 RCTs(1 RCT was omitted by sensitivity analysis)with 1040 patients were included.Overall heterogeneity was acceptable,except publication bias.Based on classical meta-analysis,JTW and JTW_(m:n)^(+)+BDZ,such as JTW_(2:1)^(+)+BDZ,JTW_(5:1)^(+)+BDZ,JTW_(6:1)^(+)+BDZ,JTW_(10:1)^(+)+BDZ,had more efficacy than BDZ.Based on network meta-analysis,JTW and JTW_(m:n)^(+)+BDZ,such as JTW_(2:1)^(+)+BDZ,JTW_(6:1)^(+)+BDZ,JTW_(10:1)^(+)+BDZ,had more efficacy than BDZ;meanwhile,SUCRA showed the best rank of JTW/JTW+(BDZ<JTW_(m:n)^(+)+BDZ<JTW)for insomina,as same as that of JTW_(m:n)+(BDZ<JTW_(5:1)^(+)+BDZ<JTW_(10:1)^(+)+BDZ<JTW_(2:1)^(+)+BDZ<JTW_(6:1)^(+)+BDZ).CONCLUSION Based on the existing clinical evidence,JTW has efficacy for insomnia,but the optimization ratio between Coptis chinensis and cinnamo of JTW_(m:n)^(+)+BDZ for insomnia needs more supports.

Pharmacokinetics of Jiaotai pill self-microemulsion in insomnia rats

Objective:The pharmacokinetics and relative bioavailability of Jiaotai pill self-microemulsion were evaluated by investigating the blood concentration of Berberine,Coptisine,Palmatine and Jatrorrhizine in insomnia rats.Methods:Insomnia rat model was established by intraperitoneal injection of p-chlorophenylalanine(PCPA).The model rats were given Jiaotai pill self-microemulsion and Jiaotai pill suspension.The contents of Berberine,Coptisine,Palmatine and Jatrorrhizine in plasma at different times after administration were determined by UPLC-MS/MS,and calculate pharmacokinetic parameters.Results:Under the set chromatographic conditions,the linear relationship of the four components was good,and the precision,accuracy and stability meet the requirements of biological samples.After intragastric administration of Jiaotai pill self-microemulsion,The C_(max) of Berberine,Coptisine,Palmatine and Jatrorrhizine were(412.68±28.45),(68.65±3.92),(34.06±3.13),(40.60±1.22)ng/mL,and AUC_(0-∞)were(672.70±72.55),(146.04±25.01),(71.49±18.67),(72.25±9.54)ng·mL^(-1)·h^(-1),respectively.Compared with Jiaotai pill suspension,the Cmax,AUC_(0-t) and AUC_(0-∞)of the four components in insomnia rats were significantly increased(P<0.01).Conclusion:Jiaotai pill self-microemulsionl can promote the absorption of effective components in insomnia rats and improve its bioavailability.

Molecular targets and mechanisms of Jiawei Jiaotai Pill on diabetic cardiomyopathy based on network pharmacology

BACKGROUND Jiawei Jiaotai Pill is commonly used in clinical practice to reduce apoptosis,increase insulin secretion,and improve blood glucose tolerance.However,its mechanism of action in the treatment of diabetic cardiomyopathy(DCM)remains unclear,hindering research efforts aimed at developing drugs specifically for the treatment of DCM.AIM To explore the pharmacodynamic basis and molecular mechanism of Jiawei Jiaotai Pill in DCM treatment.METHODS We explored various databases and software,including the Traditional Chinese Medicine Systems Pharmacology Database,Uniport,PubChem,GenCards,String,and Cytoscape,to identify the active components and targets of Jiawei Jiaotai Pill,and the disease targets in DCM.Protein-protein interaction network,gene ontology,and Kyoto Encyclopedia of Genes and Genomes analyses were used to determine the mechanism of action of Jiawei Jiaotai Pill in treating DCM.Molecular docking of key active components and core targets was verified using AutoDock software.RESULTS Total 42 active ingredients and 142 potential targets of Jiawei Jiaotai Pill were identified.There were 100 common targets between the DCM and Jiawei Jiaotai Pills.Through this screening process,TNF,IL6,TP53,EGFR,INS,and other important targets were identified.These targets are mainly involved in the positive regulation of the mitogen-activated protein kinase(MAPK)MAPK cascade,response to xenobiotic stimuli,response to hypoxia,positive regulation of gene expression,positive regulation of cell proliferation,negative regulation of the apoptotic process,and other biological processes.It was mainly enriched in the AGE-RAGE signaling pathway in diabetic complications,DCM,PI3K-Akt,interleukin-17,and MAPK signaling pathways.Molecular docking results showed that Jiawei Jiaotai Pill's active ingredients had good docking activity with DCM's core target.CONCLUSION The active components of Jiawei Jiaotai Pill may play a role in the treatment of DCM by reducing oxidative stress,cardiomyocyte apoptosis and fibrosis,and maintaining metabolic homeostasis.

Clinical Study on the Effect of Jiaotai Acupuncture Combined with Escitalopram on Mild Depression and Its Influence on Inflammatory Factors

[Objectives] To analyze the effect of Jiaotai acupuncture combined with Escitalopram on mild depression and its influence on inflammatory factors.[Methods] Eighty patients with mild depression admitted to Shenzhen Baoan District Central Hospital from March 2021 to March 2023 were randomly divided into observation group (40 cases) and control group (40 cases). The control group was treated with Escitalopram, and the observation group was treated with Jiaotai acupuncture on the basis of the control group. The overall efficacy, depression score before and after treatment, and the improvement of inflammation level were compared between the two groups.[Results] The total effective rate of the observation group was significantly higher than that of the control group ( P <0.05), and the improvement of depression score and inflammation level of the observation group was significantly better than that of the control group ( P <0.05).[Conclusions] Jiaotai acupuncture combined with Escitalopram can significantly reduce inflammation and improve symptoms in patients with mild depression.

Effect of Jiaotai Pill(交泰丸)on Intestinal Damage in Partially Sleep Deprived Rats

Objective: To explore the effect and mechanism of Jiaotai Pill(交泰丸, JTW) on intestinal mucosal damage in rats with chronic partial sleep deprivation(PSD). Methods: Obesity resistant(OR) rats were selected, and underwent 4 h PSD by being exposed to environmental noise for 4 weeks. During the whole PSD period, JTW and estazolam were orally given to the rats respectively in the treating groups. Plasma concentration of lipopolysaccharide(LPS) which is the marker of gut-origin endotoxemia was examined. Intestinal morphology changes were observed by optical microscopy. The protein expression of occludin(Ocln) in the intestine was measured by immunofluorescence technique and Western blot. The expressions of circadian proteins cryptochromes(Cry1 and Cry2) in the intestine were also determined. Results: The treatment of JTW significantly decreased LPS level in OR rats with PSD(P<0.05). JTW also attenuated insomnia-induced intestinal injury like shorter, sparse and incomplete villus, wide gap between the villus, mucosal swelling and congesting(P<0.05). These changes were associated with the effect of JTW on up-regulating the expressions of Cry1 protein, Cry2 protein and Ocln protein in the intestine. Conclusions: JTW has the beneficial effect on improving intestinal mucosal damage caused by PSD. The mechanism appears to be related to the modulation of the expressions of circadian proteins and Ocln protein in the intestine, thereby attenuating inflammation and improving insulin resistance in insomnia rats.

Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats

Objective To reveal the effect and mechanism of Jiaotai Pill(交泰丸,JTP)on insomniac rats.Methods The insomniac model was established by intraperitoneal injection of p-chlorophenylalanine(PCPA).In behavioral experiments,rats were divided into control,insomniac model,JTP[3.3 g/(kg•d)],and diazepam[4 mg/(kg•d)]groups.The treatment effect of JTP was evaluated by weight measurement(increasement of body weight),open field test(number of crossings)and forced swimming test(immobility time).A high performance liquid chromatography-electrochemical detection(HPLC-ECD)method was built to determine the concentration of monoamine transmitters in hypothalamus and peripheral organs from normal,model,JTP,citalopram[30 mg/(kg•d)],maprotiline[40 mg/(kg•d)]and bupropion[40 mg/(kg•d)]groups.Expressions of serotonin transporter(SERT),dopamine transporter(DAT),and norepinephrine transporter(NET)were analyzed by quantitative polymerase chain reaction(qPCR)and Western blot in normal,model and JTP groups.A high performance liquid chromatography-electrospray ionization mass spectrometry(HPLC-ESI-MS/MS)method was established to determine the pharmacokinetics,urine cumulative excretion of metformin in vivo,and tissue slice uptake in vitro,which were applied to assess the activity of organic cation transporters(OCTs)in hypothalamus and peripheral organs.Results Compared with the insomniac model group,the body weight and spontaneous locomotor were increased,and the immobility time was decreased after treatment with JTP(P<0.01).Both serotonin and dopamine contents in hypothalamus and peripheral organs were increased(P<0.01).The norepinephrine content was increased in peripheral organs and decreased in hypothalamus(P<0.05 or P<0.01).At the same time,SERT,DAT,OCT1,OCT2,and OCT3 were down-regulated in hypothalamus and peripheral organs(P<0.05).NET was down-regulated in peripheral organs and up-regulated in hypothalamus(P<0.05 or P<0.01).Moreover,the activity of OCTs in hypothalamus and peripheral organs was inhibited(P<0.05).Conclusion JTP alleviates insomnia through regulation of monoaminergic system and OCTs in hypothalamus and peripheral organs.

Comparative brain pharmacokinetic study of Jiaotai Pills in normal and insomnic rats using brain microdialysis combinated with LC–MS/MS

Objective：To compare the brain pharmacokinetics of five protoberberine-type alkaloids（i.e.berberine,palmatine,coptisine,epiberberine,and jatrorrhizine）,which were the main bioactive constituents of Jiaotai Pills（JTP）,in normal and insomnic rats orally administrated with JTP.Methods：The detection was conducted by a fully validated liquid chromatography-tandem mass spectrometry combinated with brain microdialysis method.Brain microdialysis probes were inserted into the hippocampus of rats.JTP extracts were administrated intragastrically and then brain microdialysates were collected at 30 min time intervals for 10 h.The separation of the five protoberberine-type alkaloids was carried out on a BDS Hypersilusing a mobile phase consisting of acetonitrile and water（containing 5 mmol ammonium acetate adjusted to p H 5.0）within 4 min.The quantification was performed by multiple reaction monitoring with the transitions of m/z 336.0-320.1 for berberine,m/z 352.0-336.1for palmatine,m/z 338.0-322.1 for jatrorrhizine,m/z 336.0-320.1 for epiberberine,m/z 320.0-292.1 for coptisine and m/z 356.4-192.1 for IS.Results：The lower limit of quantification for five protoberberine-type alkaloids was 0.05 ng/m L.Linearity,accuracy,precision,stability and matrix effect of five analytes were all satisfactory.Five protoberberinetype alkaloids were quickly distributed in the brain.Moreover,significant differences in the principal pharmacokinetic parameters such as AUC andthe analytes were observed between two groups.Conclusion：The LC-MS/MS method combinated with microdialysis is useful in the brain pharmacokinetic study of five protoberberine-type alkaloids.The results indicated that the rates of analytes absorption in insomnic rats were significantly higher than those in normal rats.Besides,the protoberberine-type alkaloids could bring a direct effect on the neuron in the hippocampus.

《交泰韵》古全浊声母清化方式研究

明代韵书《交泰韵》中古浊音声母已经清化。通过统计分析《交泰韵》阳类塞音塞擦音小韵的读音,同时结合韵书凡例的内容和反切,我们认为:中古浊塞音浊塞擦音仄声字在17世纪前后汴洛一带方言中读不送气音。吕坤为满足韵书设计的需要而制定的各种调和语音的方法,一方面说明了吕坤尊重语言事实的朴素思想,另一方面也说明旧的音韵理论已不能充分解释语言的发展变化。《交泰韵》古全浊声母清化的方式是“平声送气仄声不送气”。

1992-2022年交泰丸研究文献可视化分析

目的 使用文献计量学方法探究1992-2022年交泰丸研究现状、研究热点和研究前沿。方法 在中国知识资源总库(CNKI)中检索1992年1月1日-2022年11月10日交泰丸研究领域相关文献,统计年度发文量,使用CiteSpace6.1R3软件对纳入文献发文作者、发文机构、关键词进行知识图谱可视化分析。结果 共纳入文献345篇,年度发文量总体呈上升趋势;发文量较多的作者团队为董慧团队、全世建团队、袁琳团队及于春泉团队;发文机构以广州中医药大学及华中科技大学同济医学院附属同济医院为代表;高频关键词有“失眠”“糖尿病”“心肾不交”等;关键词聚类共分为9个聚类;“代谢组学”“糖尿病”“机制”成为最近突现的关键词。结论 近30年交泰丸研究领域处于平稳发展的阶段,糖尿病、失眠、抑郁症的治疗受到广泛关注,名医经验传承挖掘研究或可成为下一阶段的研究方向。

基于心肾相交理论辨治心律失常

通过总结、梳理杜武勋教授治疗心律失常的临床经验,从中医心肾相交理论辨析心律失常的核心病机为“心肾不交”,治疗以“交通心肾,枢转中焦”为基本准则,以脾胃为枢纽,重视调畅中焦气机,并提出以交泰丸为底方,创立了治疗本病的基础方——连桂宁心汤。意在达到降心火、温肾水之效,令水火既济,阴阳平和,心悸得复,病邪自除。为中医临床治疗心律失常疾病提供了新的思路与方法。

基于网络药理学探讨交泰丸治疗失眠的作用机制

目的通过网络药理学和分子对接技术对交泰丸治疗失眠的作用机制进行研究。方法交泰丸活性成分由中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database andAnalysis Platform,TCMSP)、中医药整合药理学研究平台(Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine,TCMIP)整合获得,SwissTargetPrediction数据库预测作用靶点,整合DisGeNET、DrugBank疾病基因数据库中失眠的靶点基因。采用Venny 2.1在线软件作图工具平台,获取活性成分与失眠靶点的交集靶,Cytoscape 3.9.1作图软件构建交泰丸活性成分的交集靶点网络,分析得到交泰丸核心成分。蛋白互作网络、核心靶点由STRING12.0数据库获得,DAVID数据进行基因本体(gene ontology,GO)功能注释和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析,采用AutoDockTools1.5.7 Pymol软件对关键靶点与成分进行分子对接、可视化处理。结果筛选得到交泰丸中治疗失眠的5个核心成分依次为小檗碱、柠檬苦素、黄柏酮、N-反式阿魏酰酪胺、黄藤素,筛选出5-羟色胺(5-hydroxy tryptamine,5-HT)转运体(solute carrier family 6 member,SLC6A4)、多巴胺受体(dopamine receptor D2,DRD2)、烟碱乙酰胆碱受体(cholinergic receptor nicotinic alpha 4 subunit,CHRNA4)、肿瘤坏死因子(tumor necrosis factor,TNF)、前列腺素内过氧化物合酶(prostaglandin-endoperoxide synthase 2,PTGS2)等核心靶点。交泰丸治疗失眠的信号通路主要富集于调节5-HT能突触、多巴胺能突触等。分子对接显示核心成分小檗碱、柠檬苦素、黄柏酮等与核心靶点SLC6A4、DRD2、CHRNA4、TNF、PTGS2等对接程度良好。结论交泰丸中小檗碱、柠檬苦酸、黄柏酮等成分通过影响SLC6A4、DRD2、TNF、CHRNA4、PTGS2等蛋白的表达,多作用机制治疗失眠。

交泰疏肝汤辅助治疗肝气郁结型2型糖尿病合并抑郁症的临床研究

目的探讨交泰疏肝汤辅助治疗肝气郁结型2型糖尿病(type 2 diabetes mellitus,T2DM)合并抑郁症的临床效果。方法选取2022年6月至2023年6月丽水市第二人民医院收治的肝气郁结型T2DM合并抑郁症患者90例,随机分为对照组和观察组,每组45例。两组患者均进行糖尿病基础治疗,对照组加用艾司西酞普兰片进行治疗,观察组在使用艾司西酞普兰片的同时加用交泰疏肝汤治疗。治疗8周后采用汉密顿抑郁量表-24(Hamilton depression scale-24,HAMD-24)评分和健康问卷抑郁量表-9(patient health questionnaire-9,PHQ-9)评分评估两组的临床疗效,测定两组患者的血清5-羟色胺(5-hydroxytrytamine,5-HT)、去甲肾上腺素(noradrenaline,NE)、脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)水平及空腹血糖(fasting blood glucose,FPG)、餐后2h血糖(2-hour postprandial blood glucose,2hPG)、糖化血红蛋白(haemoglobinA1c,HbA1c)水平,并记录不良反应。结果观察组临床总有效率高于对照组,差异有统计学意义(P<0.05)。治疗8周后,观察组患者的HAMD-24及PHQ-9评分均低于对照组(P<0.01);5-HT、NE、BDNF水平均高于对照组(P<0.01);FPG、2hPG、HbA1c水平均低于对照组(P<0.01)。两组不良反应比较差异无统计学意义(P>0.05)。结论交泰疏肝汤加减治疗肝气郁结型T2DM合并抑郁症可改善患者HAMD-24评分及PHQ-9评分,提高5-HT、NE、BDNF水平,降低FPG、2hPG、HbA1c水平,临床疗效较好且无明显不良反应。

通督交泰针法治疗卒中后肢体运动功能障碍的临床观察

目的 观察通督交泰针法治疗卒中后肢体运动功能障碍的临床疗效。方法 80例患者随机分为观察组与对照组各40例。对照组给予常规药物、针刺及相关康复训练治疗,观察组在其基础上采用通督交泰针法进行针刺治疗。比较两组患者临床疗效以及Fug1-Meyer运动功能评定量表(FMA)、日常生活能力(ADL)评分及中国临床神经功能缺损评分量表(CSS)评分。结果 观察组治疗后临床疗总有效率为86.84%,高于对照组的82.35%(P <0.05),观察组CSS评分低于对照组(P <0.05);两组患者FMA评分、ADL评分均明显升高(P <0.05),且观察组高于对照组(P <0.05)。结论 通督交泰针法对脑卒中恢复期肢体功能障碍患者临床疗效显著,对肢体运动功能具有明显改善作用,同时能提高日常生活能力。

天王补心丹与交泰丸加减联合用药在围绝经期高血压伴失眠治疗中的应用研究

目的评估天王补心丹与交泰丸加减联合用药在围绝经期高血压伴失眠治疗中的应用效果。方法回顾性选取2023年1—12月枝江市中医医院收治的100例围绝经期高血压伴失眠患者的临床资料,以不同治疗方式分组,每组50例,西医组采用常规西医治疗,中医组采用天王补心丹与交泰丸加减治疗。对比两组睡眠质量、血压水平、治疗效果。结果治疗后,中医组睡眠质量及血压水平优于西医组,差异有统计学意义(P均<0.05)。治疗后,中医组治疗有效率为98.00%(49/50),高于西医组的84.00%(42/50),差异有统计学意义(χ^(2)=4.396,P<0.05)。结论天王补心丹与交泰丸加减联合用药在围绝经期高血压伴失眠的治疗中表现出显著的临床效果,相较于常规西医治疗,该方法不仅能有效改善患者的睡眠质量,还能更好地控制血压水平,治疗有效率较高。

交泰丸治疗多囊卵巢综合征伴焦虑抑郁临床研究

目的探讨交泰丸治疗多囊卵巢综合征(polycystic ovary syndrome,PCOS)伴焦虑抑郁的临床疗效。方法选取60例PCOS伴焦虑抑郁患者,将其随机分为对照组与治疗组,每组30例。对照组给予二甲双胍片口服治疗,治疗组在对照组基础上加服交泰丸中药颗粒,2组均连续干预12周。比较2组治疗前后糖脂代谢、性激素、神经递质等指标以及焦虑、抑郁和睡眠评分等的变化。结果治疗后,2组空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting insulin,FINS)、总胆固醇(total cholesterol,TC)和甘油三酯(triglyceride,TG)等指标水平均较治疗前降低(P均<0.05),且治疗组TG和FINS明显低于对照组(P均<0.05)。2组黄体生成素(luteinizing hormone,LH)、睾酮(testosterone,T)和LH/FSH比值均较治疗前降低(P均<0.05),且治疗组T和LH/FSH降低更明显(P均<0.05)。对照组焦虑自评量表(self-rating anxiety scale,SAS)评分较治疗前降低(P<0.05),而治疗组SAS、抑郁自评量表(self-rating depression scale,SDS)评分和匹兹堡睡眠质量指数(Pittsburgh sleep quality index,PSQI)评分均较治疗前降低(P均<0.05),组间比较,治疗组降低更明显(P均<0.05)。对照组γ-氨基丁酸(γ-aminobutyric acid,GABA)较治疗前降低(P<0.05),而治疗组5-羟色胺(5-hydroxytryptamine,5-HT)和GABA均较治疗前降低(P均<0.05);与对照组比较,治疗组降低更明显(P均<0.05)。结论交泰丸和二甲双胍均能明显改善PCOS患者糖脂代谢和性激素水平;与单用二甲双胍相比,交泰丸联合二甲双胍改善TG、FINS、T和LH/FSH的效果更明显,且能明显改善患者的焦虑、抑郁评分和睡眠质量,其机制可能与调节神经递质GABA和5-HT的分泌有关。

交泰丸含药血清通过激活SIRT1抑制内质网应激改善高糖高脂对SH-SY5Y细胞的损伤

目的:探讨交泰丸改善高糖高脂对SH-SY5Y细胞损伤的可能作用机制。方法:将20只SD大鼠随机分为交泰丸组和空白组,每组10只。交泰丸组予交泰丸药液8.4 g·kg^(-1)灌胃,空白组予生理盐水灌胃,连续灌胃7 d,制备交泰丸血清和空白血清。体外培养SH-SY5Y细胞,用不同比例血清给药处理24 h后,采用CCK-8法检测交泰丸含药血清对SH-SY5Y细胞增殖活力影响;用25 mmol·L^(-1)高糖和200μmol·L^(-1)棕榈酸造模,按含药血清比例分为对照组和交泰丸含药血清低、高剂量组,SIRT1抑制剂EX527组。采用Western blot法检测各组细胞中内质网相关蛋白,肌醇需酶1α(inositol-requiring enzyme 1α,IRE1α)、磷酸化-肌醇需酶1α(phospho-inositolrequiring enzyme 1α,p-IRE1α)、蛋白激酶R样内质网激酶(protein kinase R-like endoplasmic reticulum kinase,PERK)、磷酸化-蛋白激酶R样内质网激酶(phospho-protein kinase R-like endoplasmic reticulum kinase,p-PERK)、激活性转录因子6(activating transcription factor 6,ATF6)、葡萄糖调节蛋白78(glucose regulated protein 78,GRP78)、蛋白二硫键异构酶(protein disulfide isomerase,PDI)及沉默信息调节因子1(silent information regulator 1,SIRT1)蛋白表达水平,免疫荧光检测各组细胞中关键蛋白(SIRT1、p-IRE1α、p-PERK)的表达情况。结果:与对照组相比,模型组GRP78、p-PERK与PERK比值、p-IRE1α与IRE1α比值显著升高(P<0.05),SIRT1、PDI显著降低(P<0.05);与模型组相比,交泰丸低、高浓度组SIRT1、PDI表达上调、GRP78、p-PERK与PERK比值、p-IRE1α与IRE1α比值表达下调,且呈浓度依赖性(P<0.05),EX527组显著逆转交泰丸含药血清作用。结论:交泰丸含药血清可改善高糖高脂对SH-SY5Y细胞的损伤,其作用机制可能与提高SIRT1表达,抑制内质网应激有关。

基于“心肾不交”理论探讨张晓峰教授治疗绝经前后诸证经验

绝经前后诸证是指妇女在绝经期前后出现身体及心理的一系列不适症状。相当于西医学的围绝经期综合征。张晓峰教授认为肾阴虚、心火旺,心肾不交、水火不济是其核心病机,以妇女生理特点为基础,根据围绝经期“肾气虚、天癸竭”的特殊生理,以滋阴泻火、燮理阴阳、交通心肾为治法,自拟坎离交泰汤治疗绝经前后诸证,且中医辨证为“心肾不交”型,疗效显著。

交泰神志汤临证治验探微

交泰神志汤是李智杰教授基于气机升降理论及多年临床经验,结合古方“交泰丸”创制的自拟方,有调阴阳、畅气机、交心肾之功效,并注重调节患者情志变化。其不拘泥于原方,临证辨治灵活,对心肾不交所致的不寐、心悸、健忘等神志病用之有奇效。通过真实案例分析,探讨李教授应用交泰神志汤的辨治思想与组方特点,体会“异病同治”之精髓,以期为今后临床思辨提供参考。

交泰丸治疗糖尿病脑病作用机制探讨

目的:运用网络药理学方法及分子对接技术研究交泰丸(黄连、肉桂)治疗糖尿病脑病的作用机制。方法:通过TCMSP数据库及BATMAN-TCM数据库检索交泰丸的活性成分,使用PubChem和Swiss TargetPrediction数据库进行交泰丸靶基因预测,利用GeneCards及OMIM数据库查找糖尿病脑病相关的靶基因,使用Venny2.1.0将药物靶点与疾病靶点进行映射;借助STRING平台和Cytoscape3.8.2软件绘制和PPI网络图和活性成分-作用靶点图,并通过网络拓扑参数分析得出核心活性成分和关键蛋白。对药物-疾病交集靶点进行基因本体(GO)生物过程分析和京都基因和基因组百科全书(KEGG)通路分析,使用Autodock和 PyMOL行分子对接。结果:共收集到交泰丸35个有效成分包含669个成分靶点,糖尿病脑病相关靶点1559个,药物成分和疾病的共有靶点192个,其中交泰丸治疗糖尿病脑病的关键活性成分为小檗浸碱、乙酸肉桂酯、小檗碱、木兰花碱、苯甲酸肉桂酯等,核心靶点为ALB、AKT1、GAPDH、TNF、SRC等。通路富集分析发现,交泰丸治疗糖尿病脑病主要涉及糖尿病并发症中的AGE-RAGE信号通路、脂质和动脉粥样硬化、癌症中的蛋白聚糖等通路,分子对接结果表明,交泰丸关键活性成分与核心靶点对接活性较稳定。结论:交泰丸可以通过多靶点、多通路发挥糖尿病脑病的治疗作用,其具体作用机制有待进一步地研究验证。

交泰丸对血管性痴呆模型大鼠学习记忆能力及脑组织胆碱能机制的影响

目的研究交泰丸对血管性痴呆(VD)大鼠学习记忆及脑组织胆碱能机制的影响,探讨其对VD治疗的作用及机制。方法采用线栓法制备VD大鼠模型,MG-2Y迷宫进行行为学测试,羟胺比色法测定脑组织乙酰胆碱(Ach)含量,分光光度法测定脑组织乙酰胆碱酯酶(AchE)活性,HE染色观察海马区病理变化。结果模型对照组大鼠学习记忆能力明显劣于假手术组,Ach含量降低,AchE活性升高,海马区病理改变严重;交泰丸治疗后,尤其是高剂量交泰丸组,学习记忆能力明显提高,Ach含量升高,AchE活性降低,海马区病理变化明显改善。结论交泰丸对VD大鼠学习记忆能力下降有明显的保护作用,其作用机制可能与抑制AchE活性,提高Ach含量,修复及保护缺血再灌注VD大鼠海马区神经元密切相关。