Effects of Agro-Ecological Practices on the Productivity of Orange-Fleshed Sweet Potato (Ipomoea batatas (L.) Lam) and Soil Fertility in the Sudano-Sahelian Zone of Burkina Faso

This study consisted to evaluate the effects of ecological practices on the yield performance of the JEWEL of orange-fleshed sweet potato (Ipomoea batatas (L.) Lam). The study was conducted in a Sudano-Sahelian cropping system (Lantargou, eastern region of Burkina Faso). Agro-ecological practices consisted of the inputs of 3.20 t/ha of compost + 2.45 t/ha of wood ash (CO + WA);4.90 t/ha of wood ash (WA);6.40 t/ha of compost (CO) were compared to control with no inputs (T0). Each treatment was repeated four times. The crop management consisted of plowing, harrowing, raising of ridges with 40 cm height, burying of treatments, transplanting of cuttings, two weeding’s, and using of biopesticide called PIOL for crop protection. Composite soil samples per treatment were also collected at tuber harvest and analyzed to determine the effects of treatments on residual soil fertility. Results showed that the plant heights and diameters under the CO were significantly (P < 0.001) improved by 16% and 12% compared to T0. The WA treatment significantly increased the number of large tubers by 43% (P ≤ 0.01) compared to T0. Total tuber numbers, large tuber numbers and tuber yields of sweet potato under CO + WA were significantly (P < 0.001) and respectively improved by 27%, 50% and 31% compared to T0. All treatments increased soil organic matter, N, P and K contents, and reduced soil acidity compared with those obtained under T0. Soil K content was improved by 39% under CO + WA, and soil N content by 34% under WA compared to T0. Soil C/N ratio under CO + WA was reduced by 20% compared others treatments. But, the CO + WA treatment outperformed by improving residual soil N content by 38%, and the WA treatment by increasing soil K content by 50% compared to T0. In addition, soil pHH2O increased by 1.2 units under WA treatment compared to T0. As conclusion, the application of 6.4 t/ha of compost performed well to improve the vegetative growth of orange-fleshed sweet potato while the inputs of 3.2 t/ha of compost + 2.45 t/ha of wood ash were efficacy to significantly increase the tuber yields and improve the residual fertility of soil.

Preliminary Studies on CPG/Hinf Ⅰ RFLP Groups of Citrus tristeza virus Infected Sweet Oranges in China

Citrus tristeza virus （CTV） causes economically important losses to the citrus industry worldwide. Mild strain cross protection （MSCP） against tristeza has hardly been practised due to mixed infection of different CTV-strains and little background of its molecular biology in China. For better cognition on CTV, 192 sweet orange samples collected from eight provinces （Chongqing, Sichuan, Fujian, Hunan, Guangxi, Yunnan, Guangdong and Jiangxi） were tested by direct tissue blot immuno-assay （DTBIA）, and 158 of them were tested positively, which therefore were subjected to coat protein gene （CPG）/Hinf Ⅰ restriction fragment length polymorphism （RFLP） analysis. Sample bulks were compared between Chongqing and Fujian by some statistical data, including ratios of single infection and mixed infection to local samples, proportions of CTV isolates with single RFLP groups, and rates of each RFLP group. The simplified analysis of samples from the other six provinces were then conducted. This study suggests that CTV isolates with CPG/Hinf Ⅰ RFLP groups Ⅲ and Ⅰ are the main epidemic ones in China, and mixed infection of CTV in fields are popular. Based on observation of severity of stem-pitting symptom in field trees, CTV isolates with CPG/Hinf Ⅰ RFLP groups Ⅲ and Ⅰ caused severe stem-pittings in sweet oranges in China.

Production of Transgenic Anliucheng Sweet Orange(Citrus sinensis Osbeck) with Xa21 Gene for Potential Canker Resistance

Citrus canker, an epidemic quarantine disease caused by Xanthomonas axonopodis pv. citri, has brought a great damage in citrus production worldwide. Herein, a rice PRR （pattern recognition receptor） gene Xa21 together with GUS reporter gene and hygromycin phosphotransferase gene （HPT） was introduced into Anliucheng sweet orange （Citrus sinensis Osbeck） via Agrobacterium-mediated transformation of embryogenic callus. The transgenic calluses were screened on MT basal medium containing hygromycin （HYG） and detected by histochemical GUS staining. The transgenic plantlets were recovered through somatic embryogenesis pathway. The regenerated plantlets were accustomed to and maintained in the greenhouse. The transgene integration of recovered plantlets was identiifed by PCR and Southern blot hybridization. It showed that all the transgenic plantlets tested had undergone single copy integration, the expression of Xa21 in eight different transgenic lines detected by qRT-PCR can be divided into three grades, high for T5 and T6, middle for T4 and low for the rest. The tolerance to citrus canker disease of the three recovered transgenic lines T2, T4 and T6 was assessed by in vitro pin-puncture inoculation. The results showed that all the three transgenic lines conferred improved resistance to citrus canker bacterium infection and the T4 transgenic line displayed the highest resistance. The mechanism and feasibility of rice Xa21 in triggering innate immunity in citrus was brielfy discussed.

Proteomic Comparison Between Leaves from a Red-Flesh Mutant and Its Wild-Type in Sweet Orange

The red-flesh mutant Hong Anliu sweet orange is of high nutritional value due to its lycopene accumulation.Our previous studies on this mutant fruits suggested that photosynthesis and oxidative stress could promote the formation of mutation trait.However,leaf rather than fruit is the major part for some important biological processes such as photosynthesis.In this study,we analyzed the proteomic alteration in leaves of the red-flesh mutant Hong Anliu vs.its wild type （WT）.Ten differentially expressed proteins were identified,of which two were involved in photosynthesis,three in oxidative stress,two in defense,and three in metabolism.The high up-regulation of photosynthetic proteins proved the hypothesis that enhanced photosynthesis could provide and transport more substrates into mutant fruits for carotenoid biosynthesis.Similar to the previous results in fruits,anti-oxidative proteins were highly up-regulated in leaves,suggesting the whole plant of Hong Anliu suffered from enhanced oxidative stress.Proteins involved in defense and metabolism were also identified,and their possible roles in the mutation were discussed.

International Competitiveness of Export of China’s Sweet Orange

Selecting the market share,trade competitiveness index,revealed comparative advantage( RCA),and revealed competitive advantage index( CA),this paper compared the competitiveness of China and large sweet orange exporters,and made an empirical analysis. Results indicate that in terms of the market share,Spain has prominent advantage in competitiveness of sweet orange; in terms of the trade competitiveness index,Egypt and South Africa have higher competitiveness in the export of sweet orange; in terms of RCA and CA,Egypt has absolute international advantage in the export of sweet orange. China’s export competitiveness of sweet orange is much lower than other large sweet orange producers such as Spain,Egypt,and South Africa,and the export capacity is much lower than other large sweet orange producers. In view of current situations,it came up with recommendations for improving export competitiveness of China’s sweet orange,including expanding the market,diversifying the varieties,improving the quality,reducing the costs,strengthening cooperation of fruit farmer cooperatives,and enhancing government support.

Pre-harvest Treatment of Zn ＆ B Affects the Fruit Quality and Storability of Sweet Orange

Physico-chemical composition of sweet orange （Citrus sinensis L.） cv. blood red was determined in relation to different storage conditions and micronutrients application at department of horticulture, Agricultural University Peshawar, Pakistan during 2006-2007 and 2007-2008. The post-harvest quality of sweet orange was evaluated for 60 days storage with 20 days intervals. Fruit were harvested after the foliar application of zinc and boron in two consecutive seasons. The harvested fruits were stored at an ambient temperature （ATS） of 25 ＋ 2 ℃ and at low temperature storage （LTS） of 15 ± 2 ℃ with 60%-70% relative humidity （RH） for 60 days. Sweet oranges stored at LTS maintained better fruit quality than ATS. The foliar application of zinc and boron significantly enhanced fruit juice content, total soluble solids （TSS）, ascorbic acid （AA） and non-reducing sugar （NRS） of fruit. However, fruit juice content, TSS and AA were observed significantly higher, when the fruit was treated with high zinc （1%） and low boron （0.02%）. The percent of weight loss, disease incidence, TSS and reducing sugar （RS） increased with increasing the storage durations. A reduction was noted in fruit juice, AA and NRS with increasing the storage durations.

Evaluation of Kenyan Orange Fleshed Sweet Potato （Ipomoea batatas Lam,） Purees for Functional Food Production

Consumption of functional foods with health promoting benefits and/or disease prevention has been on the increase globally. The study aimed at evaluating the potential of utilizing Kenyan orange fleshed sweet potato （Ipomoea batatas Lam.） for functional food production with aesthetics benefits. Purees from three different varieties of orange fleshed sweet potato （OFSP） were analyzed for nutritional, physicochemical and microbial quality, The findings of the study show that the three purees were all microbiologically safe and of near neutral pH, but differed significantly （P 〈 0.05） in nutrient content （dry matter content, 12.76-28.23%; crude fiber, 1.37-2.90% fresh weight basis （FWB）; 13-carotene, 0.94-9.27 mg/100g dry weight basis （DWB）; starch, 10.20-18.30% FWB ＆ total sugar 27.08-31.76% DWB）. The purees had attractive appearance （ranging from yellow to dark orange）, with varying significantly different spectrophotometer hunters color scale （P 〈 0.05）, and flow ability. Conclusions from the findings show great potential of utilizing the varying properties of OFSP puree to produce enriched functional food products such as probiotic/prebiotic dairy, beverages, bakery and baby foods.

Improved Energy and Sensory Properties of Instant Porridge Made from a Roasted Mixture of Grated Orange-Fleshed Sweet Potatoes and Flour Made from Shredded Sun Dried Cassava

Mozambican diets are often lacking in vitamin A. Orange-fleshed sweet potatoes (OFSP) are rich in beta-carotene, a precursor of vitamin A, but are not easily introduced into the diet as the consistency of the porridge made from OFSP is unappetising. Flour made from roasted cassava (garri) produces a palatable porridge with a high energy density. We propose a simple procedure for producing an instant porridge by roasting grated OFSP with flour from shredded sun-dried cassava (G-OFSP). This is an easily adopted variant of the traditional garri-making process. The consistency of this porridge made from milled G-OFSP (G-OFSPf) was compared to the consistency of porridges made from maize flour (Mf), cassava flour (Cf), OFSP flour (OFSPf) and garri flour (Gf) at 43°C, and then correlated to the perceived characteristics assessed by a sensory panel. The dry matter concentration of the porridges was adjusted to give similar consistency, measured by the maximum force of back extrusion, using traditional maize porridge as a reference. The porridges were additionally characterized by back extrusion force relaxation time. Short relaxation times were obtained for G-OFSPf, Mf and Gf porridges and longer times for Cf and OFSPf porridges, corresponding roughly to the sensory preference. In the sensory test of consistency, G-OFSPf porridge was ranked first, followed by Mf, Gf, OFSPf and Cf. In overall sensory tests comparing G-OFSPf porridge to Gf porridge, G-OFSPf porridge was significantly preferred with regard to all sensory attributes evaluated (p < 0.01). The energy density of the G-OFSPf porridge was 5.6 kJ/ml;the highest of the three most preferred porridges with regard to texture. The preparation of flour from a mixture of grated OFSP and shredded sun-dried cassava improved the perceived porridge consistency, increased its energy density, and its high acceptability would increase the intake of betacarotene.

甜橙精油-北五味子提取物复合膜的制备及其在冷鲜猪肉保鲜中的应用

为提高冷鲜猪肉的保鲜能力并延长其货架期,以甜橙精油(orange essential oil,OEO)、北五味子提取物(Schisandra chinensis extract,SCE)为活性成分,羧甲基纤维素钠(sodium carboxymethyl cellulose,CMC-Na)为成膜基质,采用流延法制备复合膜。利用单因素试验和正交试验优选复合膜的配制方法,以色泽、汁液流失率、pH值、感官评价、总挥发性盐基氮含量和菌落总数探究OEO-SCE复合膜对冷鲜猪肉保鲜能力的影响。结果表明,当CMC-Na添加量为1.0 g/100 mL、SCE添加量为0.9 g/100 mL、OEO添加量为0.2%(V/V)时,制备的复合膜综合性能最佳:断裂伸长率为(63.860±0.120)%,抗拉强度为(8.800±0.049)MPa,水蒸气透过率为(2.360±0.070)g·mm/(m^(2)·h·kPa)。用该膜包裹冷鲜猪肉进行保鲜,与未包膜组相比,色泽及感官评分有明显提升,可以有效降低贮藏期内的总挥发性盐基氮含量、pH值、汁液流失率及菌落总数,贮藏期从6 d延长至8 d。由此可见,OEO-SCE复合膜可以抑制冷鲜猪肉在贮藏期内的腐败变质并延长其贮藏期。

柑橘溃疡病菌胁迫对海南绿橙植株生理指标与抗氧化酶的影响

分析溃疡病菌对海南绿橙植株生长代谢和抗氧化酶系统的影响将有助于研究其在溃疡病菌胁迫下的调控机制,推进抗性品种选育工作。通过盆栽试验,主要研究海南绿橙在溃疡病菌胁迫下的生理代谢变化及关键抗氧化酶的响应。结果表明,溃疡病菌胁迫下,绿橙植株的株高增长量、叶面积均未受到显著影响,但植株鲜重显著下降,且引起绿橙叶片的黄化,其中叶绿素a、叶绿素b含量的下降幅度分别为74.09%、81.75%。在溃疡病菌胁迫的0~72 h,超氧化物歧化酶(SOD)、苯丙氨酸解氨酶(PAL)活性和叶片细胞中的丙二醛(MDA)含量均在胁迫24 h时达到高峰,分别是胁迫前的6.15、5.08、5.60倍,以上指标均高于空白对照;冗余分析(RDA)显示,酶活的变化量与各生理指标保持着较好的负相关关系。综上,溃疡病菌胁迫主要引起绿橙叶片光合色素含量的降低,影响叶片的光合代谢,而SOD和PAL是绿橙植株应对溃疡病菌侵染时的关键抗氧化酶,研究结果将为海南柑橘抗性育种的定向选择提供理论研究基础。

甜橙精油穴位按摩对老年人气阴两虚型功能性便秘的影响

目的:观察甜橙精油穴位按摩对气阴两虚型功能性便秘(FC)老年人的影响。方法:采用便利抽样法,选取2021年12月—2022年12月甘肃省中医院老年病科收治的75例气阴两虚型功能性便秘老年人为研究对象,随机将其分为对照组(n=37)和试验组(n=38)。对照组在常规护理的基础上给予单纯穴位按摩,试验组在常规护理的基础上给予甜橙精油穴位按摩,疗程均为2周。干预2周后比较两组周自发完全排便次数、Bristol粪便性状评分及便秘病人症状自评量表(PAC-SYM)评分。结果:干预2周后,试验组自发完全排便次数、Bristol粪便性状评分高于对照组,PAC-SYM评分低于对照组,差异均有统计学意义(P<0.05)。结论:甜橙精油穴位按摩应用于气阴两虚型功能性便秘老年人的疗效优于单纯穴位按摩,能够增加病人的排便次数,改善粪便性状,减轻便秘相关症状。

甜橙香精微胶囊的制备及缓释性能研究

为了改善香精微胶囊的缓释性能、提高香精利用率,以甜橙香精为芯材、六亚甲基二异氰酸酯(HDI)三聚体为合成壁材的单体、聚乙二醇(PEG)和石墨烯(GR)作为温控材料,利用界面聚合法制备温控香精微胶囊(egHDI-O),考察芯壁比、剪切乳化机的转速、温控组分对甜橙香精微胶囊形貌、组成、芯材含量和缓释性能的影响。结果表明:在甜橙香精微胶囊芯壁比为1∶2,转速为8000 r/min,乳化剂为吐温80,PEG和GR用量分别为200 g/L和5 g/L工艺条件下制备的egHDI-O具有良好的形貌和均匀的粒径,其芯材含量(质量分数)可达74%;相比于未添加PEG和GR的微胶囊,egHDI-O具有优良的温控性能。研究为开发新型温控缓释赋香材料提供了新思路。

冻干白茶甜橙块配方研究

【目的】目前,白茶主要以冲泡饮用为主,市场上可供选择的白茶食品有限。本研究以速溶白茶粉和甜橙为主要原料,研制一款具有独特风味的新型白茶食品。【方法】通过单因素实验和正交试验,结合感官评分考察了速溶白茶粉、甜橙果酱和甜橙香精对冻干白茶甜橙块感官品质的影响,优化了冻干白茶甜橙块的配方。【结果】冻干白茶甜橙块的最佳工艺配方为速溶白茶粉0.1 g、甜橙果酱5 g、甜橙香精0.5 g、冰糖5 g、白砂糖5 g、糊精5 g、果胶0.5 g和水72 g。【结论】在此配方条件下可制得茶香、橙香和柚香浓郁协调,酸甜可口的冻干白茶甜橙块。

^(60)Co-γ射线联合甜橙精油微胶囊对小龙虾的保鲜作用

目的探究辐照联合甜橙精油微胶囊对4℃贮藏温度下小龙虾品质的影响。方法将甜橙精油微胶囊同新鲜去壳小龙虾肉一起封装,经^(60)Co-γ射线辐照6kGy处理后贮存于4℃冷藏中。以挥发性盐基氮(total volatile basic nitrogen,TVB-N)值、硫代巴比妥酸(thiobarbituric acid,TBA)值、pH、感官评价、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate polyacrylamide gel electrophoresis,SDS-PAGE)、扫描电镜(scanning electron microscope,SEM)肌肉微观组织结构分析以及菌落总数为品质指标对保鲜效果进行评价。结果经^(60)Co-γ射线联合甜橙精油微胶囊对小龙虾保鲜处理后,第8 d时TVB-N值为16.73 mg/100 g,菌落总数为5.42lgCFU/g,TBA值为0.561mg/kg,均未超过可食用标准,而对照组已不可食用,且第10d时pH为7.7,各项指标明显好于对照组。结论说明^(60)Co-γ联合甜橙精油微胶囊对小龙虾具有较好的保鲜效果,且在4℃冷藏下,对小龙虾的货架期可以延长至7 d。

甜橙油与马铃薯淀粉复配对鱼糜制品品质的影响

为研究甜橙油与马铃薯淀粉复配对鱼糜制品品质的影响,分别以0,0.2,0.4,0.6 mL/100 g的甜橙油(按鱼糜质量计算)与马铃薯淀粉复配加入鱼糜中制成凝胶,对其凝胶强度、质构特性、持水性、色度、化学作用力进行分析,并用电子鼻、电子舌和感官评分进行感官品质研究。结果显示,0.4 mL/100 g的甜橙油与马铃薯淀粉复配制得的鱼糜凝胶具有最佳的凝胶强度与持水性,同时具有最高的白度值;甜橙油添加量为0.6 mL/100 g时凝胶性能次之。甜橙油与马铃薯淀粉复配对鱼糜制品的气味与滋味产生明显影响(P<0.05),并可降低酸味与增加甜味。综合分析表明0.4 mL/100 g的甜橙油与马铃薯淀粉复配可提高鱼糜凝胶整体品质,感官评分总分最高具有较好的可接受性。

利用SLAF-seq简化基因组数据挖掘甜橙果实品质性状基因

【目的】通过对240份不同甜橙资源群体利用SLAF-seq测序数据进行Fst与XP-CLR选择性清除分析,挖掘调控甜橙中优良性状的相关基因,为甜橙的分子育种工作提供重要参考。【方法】对国家柑橘种质资源圃(重庆)中保存的具有广泛遗传多样性和不同地理来源的240份甜橙种质资源进行SLAF-seq测序,获得全基因组范围内的单核苷酸多态性(single nucleotide polymorphism,SNP)基因型数据和单果重、果脐有无、可滴定酸含量等性状的表型数据,分析不同亚群之间Fst与XP-CLR数值。【结果】采用SLAF-seq技术对240份甜橙进行测序,共获得497.82 Mb短读数据,用BWA软件将测序数据比对到甜橙参考基因组上,取GATK和samtools两种方法得到的SNP标记交集,共得到1 467 968个SNP。利用Fst与XP-CLR分析筛选出了与甜橙果脐有无、单果重以及可滴定酸相关的候选基因。对受选择SNP位点附近区域进行基因注释,结果表明基因orange1.1g044639m和orange1.1g023641m参与编码生长素外排载体,可能与脐的形成相关;orange1.1g023641m在单果重性状中的Fst得分最高,其参与编码E3泛素连接酶,可能影响果实大小和重量,orange1.1g046891m可能通过调节碳水化合物的形成来影响果实重量;orange1.1g011684m编码丙酮酸脱氢酶二氢硫辛酰胺转乙酰基酶,其CDS序列发生碱基突变,可能影响了柠檬酸在不同品种间的含量;orange1.1g034502编码丝氨酸/苏氨酸蛋白激酶,基因注释功能与磷酸化/去磷酸化相关。【结论】本研究利用Fst方法对甜橙的SLAF-seq数据进行筛选。最终在甜橙资源的有无果脐、单果重和可滴定酸等性状上筛选出了6个相关的基因,可作为后期验证的候选基因,为甜橙品种的定向改良提供了依据。

冰糖橙叶片受柑橘溃疡病菌诱导脱落的离区解剖特性分析

【目的】观察冰糖橙叶片受柑橘溃疡病菌(Xanthomonas citri subsp.citri,Xcc)诱导脱落过程中离区组织结构的动态变化特征,探明冰糖橙叶片受Xcc诱导脱落的特征和规律。【方法】通过不同的活体接种方式和接种不同浓度的病原菌,筛选加速叶片脱落的Xcc接种方式和接种浓度,并采用树脂切片观察叶片脱落过程中离区组织结构变化。【结果】与注射接种10^(6)或10^(7)CFU·mL^(-1)Xcc、10^(8)CFU·mL^(-1)主要致病效应子pthA4缺失的菌系Xcc 049A,以及浸泡接种10^(8)或10^(9)CFU·mL^(-1)Xcc相比,注射接种10^(8)CFU·mL^(-1)Xcc显著促进本叶脱落,翼叶未脱落,4 dpi(day post inoculation)离区细胞分化形成离层和保护层,离区细胞膨大且细胞平均面积减少。【结论】冰糖橙叶片注射接种10^(8)CFU·mL^(-1)Xcc后,在本叶和翼叶衔接处从外周向中轴逐渐分离,分化形成成熟的离层和保护层,最终导致本叶脱落。

辐射诱变冰糖橙早熟突变体的遗传鉴定与品种特性

为探究辐射诱变冰糖橙果实提早着色性状的遗传变异,果实着色与糖酸内在品质表现,以及辐射诱变冰糖橙是否为早熟突变体,以突变体与对照冰糖橙为材料,对比分析春梢长度、春梢叶片、花器官、果实大小、果皮颜色转变、主要物候期等性状和特性的变化,应用分子标记鉴定遗传变异,比较两者果实中可溶性固形物、可滴定酸、固酸比的变化以及蔗糖和柠檬酸代谢相关基因的表达。结果表明,与对照冰糖橙相比,突变体春梢显著变短,成熟时果实较大,果皮和果肉提前15 d转色成橙黄色和橙色,主要物候期提早2~5 d,插入或缺失(InDel)标记和简单序列重复(SSR)标记引物扩增都存在差异条带,突变体发生了遗传改变。早熟突变体果实外观颜色与内在品质在花后190 d同步达到正常成熟度,可溶性固形物、可滴定酸及固酸比分别为14.53°Brix、0.46%和31.59,表现为低酸、降酸早、固酸比值高,比对照提早15 d成熟。蔗糖和柠檬酸的合成、降解、转运相关基因的表达分析结果表明,早熟突变体蔗糖合成多、转运早,柠檬酸合成少、降解快、贮运少,与果实品质表现低酸、降酸早、固酸比高基本吻合。综上,辐射诱变突变体发生了植物性状、生物特性、DNA的遗传改变,果实降酸早、含酸低、固酸比高,11月上旬着色成熟。研究结果为该突变体的进一步选育和登记早熟冰糖橙新品种奠定了基础。

AM菌发酵水肥对冰糖橙品质的影响

为研究AM菌发酵水肥对冰糖橙品质的影响,采用水肥一体化智能过滤滴灌系统在永兴县4地(BL、BJ、HN、TH)同步进行AM菌发酵水肥替代化肥的试验,考察冰糖橙的单果重、果皮厚度、果形指数等外在品质以及果实可溶性固形物、可滴定酸、固酸比、果实VC含量、可食率和出汁率等内在品质。结果表明:在冰糖橙水肥一体化种植管理中使用AM菌发酵水肥替代化肥,不会对冰糖橙单果重、果皮厚度及果形指数造成显著影响,对果实可食率、出汁率也几乎没有影响,但是能提升果实中可溶性固形物含量(增幅为16.46%~33.80%),降低可滴定酸含量(降幅为4.55%~31.43%),提高固酸比(增幅为54.32%~63.18%),显著提升其内在品质;而对果实中VC含量的影响在不同试验点表现不一致,需要结合土壤养分、种植管理、光照气候等因素作进一步探究。

甘桔冰梅片联合嗓音训练在声带小结患者中的应用效果观察

目的 分析甘桔冰梅片联合嗓音训练在声带小结患者中的应用效果。方法 选取2019年6月至2021年5月南阳市第一人民医院西区医院89例声带小结患者,随机数字表法分为两组,对照组(44例)接受甘桔冰梅片治疗,研究组(45例)予以甘桔冰梅片联合嗓音训练干预。干预8周后比较两组声带小结明显缩小率、干预前后嗓音声学指标[基频微扰(jitter)、基频(F0)、振幅微扰(shimmer)]、发声时间[最长发声时间(MPT)、最大音量最长发声时间(MLPT)]、中文版嗓音障碍指数(VHI)量表评分。结果 研究组声带小结明显缩小率高于对照组,差异有统计学意义(P<0.05)。干预后,研究组F0水平较对照组升高,Jitter、Shimmer水平较对照组低,MPT、MLPT均长于对照组,VHI评分低于对照组,差异有统计学意义(P<0.05);研究组就医体验满意度VAS评分高于对照组,差异有统计学意义(P<0.05)。结论 甘桔冰梅片联合嗓音训练应用于声带小结,可明显缩小患者声带小结,改善嗓音声学指标,延长发声时间,促进患者嗓音障碍恢复,提高患者就医体验满意度。