Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic h...Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic human lung carcinoma cell line, by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) cell viability assay, and to study the change of intracellular calcium concentration, connexin43 (Cx43) expression, gap junctional intercellular communication (GJIC) and cell cycle distribution after the tea polyphenol treatment by laser scanning confocal microscopy and flow cytometry. The results showed that 1) tea polyphenol could kill the PG cells in a dose-depent manner via inhibiting the PG cell proliferation and blocking the PG cell cycle progression staying in G0/G1 phase and not transfering in S and G2/M phases to reduce the PG cell proliferation index;2) the increases of intracellular calcium concentration, GJIC and Cx43 expression were related with the tea polyphenol doses. The data suggested that tea polyphenol could inhibit the growth of PG cells, which mechanism was associated with the up-regulation of GJIC.展开更多
For discrimination of the common W-junction and Y-junction in the labels of line drawing for a three-dimensional manifold plane surface with trihedral vertices, the existing algorithms are almost using angles between ...For discrimination of the common W-junction and Y-junction in the labels of line drawing for a three-dimensional manifold plane surface with trihedral vertices, the existing algorithms are almost using angles between two adjacent lines and W-junction and Y- junction＇s definitions, and other complex methods. This passage gives four methods with their detailed mathematical inference processes in order to use these algorithms implemented by computer. The algorithms listed are Angle Summation method and Points to the Line method, Triangle methods which are the Triangle Area method and the Cross method. When the computer programs discriminate junctions, by comparison, the Angle Summation method and Points to the Line method are less efficacious than the Triangle method, and Cross method is more efficacious than the Triangle Area method in the Triangle method.展开更多
目的:探讨成人脊柱畸形(adult spinal deformity,ASD)矫形术后早期躯体重心线(W-line)与近端交界区失败(proximal junctional failure,PJF)的相关性。方法:回顾性分析2014年6月~2019年3月在我院行手术治疗的99例ASD患者的临床资料,其中...目的:探讨成人脊柱畸形(adult spinal deformity,ASD)矫形术后早期躯体重心线(W-line)与近端交界区失败(proximal junctional failure,PJF)的相关性。方法:回顾性分析2014年6月~2019年3月在我院行手术治疗的99例ASD患者的临床资料,其中男19例,女80例。均行单纯后路长节段(固定椎体≥4个)固定融合手术,术后随访至少满2年。测量并记录所有患者术前、术后3个月及末次随访时的脊柱-骨盆参数。在脊柱全长侧位X线片上,将经外耳道口垂线定义为W-line,根据W-line不同位置分为A组、B组及C组。测量术后3个月时W-line到S1后上角水平距离(WSD)及W-line到股骨头中心连线中点的水平距离(WHD),将W-line位置进行数值转化(the numeric value of W-line,N-W-line):N-W-line=WHD/WSD。W-line位于参考点前方数值为正,反之为负。采用受试者工作特征(receiver operating characteristic,ROC)曲线分析得出N-W-line临界值。三组患者参数进行方差分析,应用Kaplan-Meier曲线分析非PJF生存时间。结果:ROC曲线分析得出术后3个月N-W-line临界值为±0.78[敏感度=75%,特异度=75.9%,曲线下面积(the area under the curve,AUC)=0.736,P=0.003]。A组32例,N-W-line>0.78;B组49例,-0.78≤N-W-line≤0.78;C组18例,N-W-line<-0.78。A组患者骨盆入射角(pelvic incidence,PI)最小(P<0.05),术后3个月矢状面平衡距(P<0.001)、T1骨盆角(P<0.001)及骨盆腰椎匹配度(P<0.001)均显著小于其他两组患者。但随访中,A、B及C组分别有12例、3例及1例患者出现PJF,A组患者PJF发生率显著高于其他两组(12/32 vs 3/49 vs 1/18,P<0.001)。此外,A组患者非PJF生存时间显著低于其他两组患者(P=0.005,Log-rank检验)。B、C组患者W-line位置逐渐前移,脊柱-骨盆矢状位参数逐渐增大,但术后PJF发生率显著降低。结论:ASD患者矫形术后早期W-line位置可有效预测PJF发生。矫形手术后W-line偏后或过度偏前,术后随访中PJF发生率显著增加,矫形术后躯干呈轻度前屈为最佳。展开更多
Although line drawings consist of only line segments on a plane, they convey much information about the three-dimensional object structures. For a computer interpreting line drawings, some intelligent mechanism is req...Although line drawings consist of only line segments on a plane, they convey much information about the three-dimensional object structures. For a computer interpreting line drawings, some intelligent mechanism is required to extract three-dimensional information from the two-dimensional line drawings. In this paper, a new labeling theory and method are proposed for the two-dimensional line drawing with hidden-part-draw of a three-dimensional planar object with trihedral vertices. Some rules for labeling line drawing are established. There are 24 kinds of possible junctions for line drawing with hidden-part-draw, in which there are 8 possible Y and 16 W junctions. The three problems are solved that Sugihara＇s line drawing labeling technique exists. By analyzing the projections of the holes in manifold planar object, we have put forward a labeling method for the line drawing. Our labeling theory and method can discriminate between correct and incorrect hidden-part-draw natural line drawings. The hidden-part-draw natural line drawings can be labeled correctly by our labeling theory and method, whereas the labeling theory of Sugihara can only label the hidden-part-draw unnatural line drawings in which some visible lines must be drawn as hidden lines, and some invisible lines must be drawn as continuous lines.展开更多
文摘Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic human lung carcinoma cell line, by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) cell viability assay, and to study the change of intracellular calcium concentration, connexin43 (Cx43) expression, gap junctional intercellular communication (GJIC) and cell cycle distribution after the tea polyphenol treatment by laser scanning confocal microscopy and flow cytometry. The results showed that 1) tea polyphenol could kill the PG cells in a dose-depent manner via inhibiting the PG cell proliferation and blocking the PG cell cycle progression staying in G0/G1 phase and not transfering in S and G2/M phases to reduce the PG cell proliferation index;2) the increases of intracellular calcium concentration, GJIC and Cx43 expression were related with the tea polyphenol doses. The data suggested that tea polyphenol could inhibit the growth of PG cells, which mechanism was associated with the up-regulation of GJIC.
文摘For discrimination of the common W-junction and Y-junction in the labels of line drawing for a three-dimensional manifold plane surface with trihedral vertices, the existing algorithms are almost using angles between two adjacent lines and W-junction and Y- junction＇s definitions, and other complex methods. This passage gives four methods with their detailed mathematical inference processes in order to use these algorithms implemented by computer. The algorithms listed are Angle Summation method and Points to the Line method, Triangle methods which are the Triangle Area method and the Cross method. When the computer programs discriminate junctions, by comparison, the Angle Summation method and Points to the Line method are less efficacious than the Triangle method, and Cross method is more efficacious than the Triangle Area method in the Triangle method.
文摘目的:探讨成人脊柱畸形(adult spinal deformity,ASD)矫形术后早期躯体重心线(W-line)与近端交界区失败(proximal junctional failure,PJF)的相关性。方法:回顾性分析2014年6月~2019年3月在我院行手术治疗的99例ASD患者的临床资料,其中男19例,女80例。均行单纯后路长节段(固定椎体≥4个)固定融合手术,术后随访至少满2年。测量并记录所有患者术前、术后3个月及末次随访时的脊柱-骨盆参数。在脊柱全长侧位X线片上,将经外耳道口垂线定义为W-line,根据W-line不同位置分为A组、B组及C组。测量术后3个月时W-line到S1后上角水平距离(WSD)及W-line到股骨头中心连线中点的水平距离(WHD),将W-line位置进行数值转化(the numeric value of W-line,N-W-line):N-W-line=WHD/WSD。W-line位于参考点前方数值为正,反之为负。采用受试者工作特征(receiver operating characteristic,ROC)曲线分析得出N-W-line临界值。三组患者参数进行方差分析,应用Kaplan-Meier曲线分析非PJF生存时间。结果:ROC曲线分析得出术后3个月N-W-line临界值为±0.78[敏感度=75%,特异度=75.9%,曲线下面积(the area under the curve,AUC)=0.736,P=0.003]。A组32例,N-W-line>0.78;B组49例,-0.78≤N-W-line≤0.78;C组18例,N-W-line<-0.78。A组患者骨盆入射角(pelvic incidence,PI)最小(P<0.05),术后3个月矢状面平衡距(P<0.001)、T1骨盆角(P<0.001)及骨盆腰椎匹配度(P<0.001)均显著小于其他两组患者。但随访中,A、B及C组分别有12例、3例及1例患者出现PJF,A组患者PJF发生率显著高于其他两组(12/32 vs 3/49 vs 1/18,P<0.001)。此外,A组患者非PJF生存时间显著低于其他两组患者(P=0.005,Log-rank检验)。B、C组患者W-line位置逐渐前移,脊柱-骨盆矢状位参数逐渐增大,但术后PJF发生率显著降低。结论:ASD患者矫形术后早期W-line位置可有效预测PJF发生。矫形手术后W-line偏后或过度偏前,术后随访中PJF发生率显著增加,矫形术后躯干呈轻度前屈为最佳。
文摘Although line drawings consist of only line segments on a plane, they convey much information about the three-dimensional object structures. For a computer interpreting line drawings, some intelligent mechanism is required to extract three-dimensional information from the two-dimensional line drawings. In this paper, a new labeling theory and method are proposed for the two-dimensional line drawing with hidden-part-draw of a three-dimensional planar object with trihedral vertices. Some rules for labeling line drawing are established. There are 24 kinds of possible junctions for line drawing with hidden-part-draw, in which there are 8 possible Y and 16 W junctions. The three problems are solved that Sugihara＇s line drawing labeling technique exists. By analyzing the projections of the holes in manifold planar object, we have put forward a labeling method for the line drawing. Our labeling theory and method can discriminate between correct and incorrect hidden-part-draw natural line drawings. The hidden-part-draw natural line drawings can be labeled correctly by our labeling theory and method, whereas the labeling theory of Sugihara can only label the hidden-part-draw unnatural line drawings in which some visible lines must be drawn as hidden lines, and some invisible lines must be drawn as continuous lines.
