The POU domain transcription factor Oct4 is a master regulator in maintaining self-renewal and pluripotency of embryonic stem(ES) cells.To further explore the functional network of Oct4,the yeast two-hybrid system was...The POU domain transcription factor Oct4 is a master regulator in maintaining self-renewal and pluripotency of embryonic stem(ES) cells.To further explore the functional network of Oct4,the yeast two-hybrid system was used to search for Oct4 interacting proteins.PH domain(containing POU domain and homeodomain) of human OCT4 was used as a bait.From the human testis cDNA library,we identi-fied a strong interaction between OCT4 and karyopherin-alpha 2(KPNA-2).KPNA2 is involved in active nuclear import of proteins.This finding was confirmed by glutathione S-transferase pull-down and co-immunoprecipitation assays.The interaction between OCT4 and KPNA-2 was further mapped to multiple regions of the two proteins.In addition,we studied nuclear localization signal(NLS) of mouse Oct4 and demonstrated that it is essential for Oct4 nuclear localization.Thus,our data suggest that Oct4 nuclear localization may be mediated by its interaction with KPNA-2.展开更多
结合储层CO_(2)埋存技术,自主搭建了地层温度压力条件下CO_(2)埋存实验装置,开展了多介质辅助CO_(2)埋存实验研究。研究结果表明,乙醇-KOH体系能够有效进行CO_(2)矿化埋存,其中96%乙醇+3 g KOH 500 mL溶液捕集CO_(2)能力最强,是最佳的CO...结合储层CO_(2)埋存技术,自主搭建了地层温度压力条件下CO_(2)埋存实验装置,开展了多介质辅助CO_(2)埋存实验研究。研究结果表明,乙醇-KOH体系能够有效进行CO_(2)矿化埋存,其中96%乙醇+3 g KOH 500 mL溶液捕集CO_(2)能力最强,是最佳的CO_(2)矿化埋存溶液配比。经CO_(2)矿化埋存后,低渗透岩心孔隙度平均降低7.07%,孔隙度变化率与孔隙度呈正相关关系,渗透率平均降低16.01%。因此,96%乙醇+3 g KOH能够加速CO_(2)在储层中的CO_(2)沉淀过程,缩短CO_(2)在储层中的矿化埋存时间。该研究可重复性、准确性和可扩展性较强,能够激发学生自主设计实验的积极性及创新意识,培养学生的独立思考能力,有利于学生将理论知识与实际工程问题相结合,实现科研能力与创新能力的相互促进。展开更多
基金supported by the Shanghai Science & Technology Developmental Foundations (No.06dj14001)the National High Technology Research and Development Program of China (No. 2006AA02Z197, 2006CB943901, and 2007CB947904)
文摘The POU domain transcription factor Oct4 is a master regulator in maintaining self-renewal and pluripotency of embryonic stem(ES) cells.To further explore the functional network of Oct4,the yeast two-hybrid system was used to search for Oct4 interacting proteins.PH domain(containing POU domain and homeodomain) of human OCT4 was used as a bait.From the human testis cDNA library,we identi-fied a strong interaction between OCT4 and karyopherin-alpha 2(KPNA-2).KPNA2 is involved in active nuclear import of proteins.This finding was confirmed by glutathione S-transferase pull-down and co-immunoprecipitation assays.The interaction between OCT4 and KPNA-2 was further mapped to multiple regions of the two proteins.In addition,we studied nuclear localization signal(NLS) of mouse Oct4 and demonstrated that it is essential for Oct4 nuclear localization.Thus,our data suggest that Oct4 nuclear localization may be mediated by its interaction with KPNA-2.
文摘结合储层CO_(2)埋存技术,自主搭建了地层温度压力条件下CO_(2)埋存实验装置,开展了多介质辅助CO_(2)埋存实验研究。研究结果表明,乙醇-KOH体系能够有效进行CO_(2)矿化埋存,其中96%乙醇+3 g KOH 500 mL溶液捕集CO_(2)能力最强,是最佳的CO_(2)矿化埋存溶液配比。经CO_(2)矿化埋存后,低渗透岩心孔隙度平均降低7.07%,孔隙度变化率与孔隙度呈正相关关系,渗透率平均降低16.01%。因此,96%乙醇+3 g KOH能够加速CO_(2)在储层中的CO_(2)沉淀过程,缩短CO_(2)在储层中的矿化埋存时间。该研究可重复性、准确性和可扩展性较强,能够激发学生自主设计实验的积极性及创新意识,培养学生的独立思考能力,有利于学生将理论知识与实际工程问题相结合,实现科研能力与创新能力的相互促进。