Background:Using network pharmacology to explore the potential molecular mechanism of traditional Chinese medicine in treating polycystic ovary syndrome(PCOS)with kidney deficiency and blood stasis syndrome.Method:Col...Background:Using network pharmacology to explore the potential molecular mechanism of traditional Chinese medicine in treating polycystic ovary syndrome(PCOS)with kidney deficiency and blood stasis syndrome.Method:Collect the related literature materials of PCOS with kidney deficiency and blood stasis syndrome treated by traditional Chinese medicine in four databases in recent ten years,extract the information of prescriptions and complete the frequency analysis.Traditional Chinese Medicine Systems Pharmacology Database was used to screen out the effective components.Use Online Mendelian Inheritance in Man and other databases to screen PCOS disease targets.The intersection targets obtained by clustering prescription and PCOS disease targets were submitted to STRING database for protein-protein interaction network analysis,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes pathways were analysed by Metascape.Result:There are 155 kinds of traditional Chinese medicines used in the literature.The most commonly utilized ones are Cuscutae Semen,Angelicae Sinensis Radix,and Rehmanniae Radix Praeparata.The results of the cluster analysis indicated that the plants most commonly found throughout the prescription were Leonuri Herba,Lycopi Herba,Dipsaci Radix,etc.GO results show that biological processes include cell reaction to organic nitrogen compounds and cell reaction to nitrogen compounds.The functional display of GO molecule includes cytokine receptor binding,signal receptor regulator activity and so on.Kyoto Encyclopedia of Genes and Genomes results show that the possible mechanisms of action are cancer pathway,an endocrine resistance signal pathway.Conclusion:Through data mining,the cluster prescription for PCOS with kidney deficiency and blood stasis syndrome is Leonuri Herba,Lycopi Herba,Dipsaci Radix,etc.The network pharmacology research of cluster prescription shows that the main drug components for treating PCOS with kidney deficiency and blood stasis syndrome are quercetin,kaempferol,luteolin,tanshinone IIA,etc.,which act on PTGS2,NCOA2,and other targets,and treat PCOS with kidney deficiency and blood stasis syndrome through cancer and endocrine resistance.展开更多
Objective:To observe the clinical efficacy of compound of owarming yang,descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome.Meth...Objective:To observe the clinical efficacy of compound of owarming yang,descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome.Methods:Seventy-six patients of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome were randomly divided into observation group and control group,thirty-eight cases in each group.The control group was given conventional western medicine treatment,while the observation group took compound of owarming yang,descending turbidity and dredging collaterals orally on the basis of conventional western medicine treatment.The course of treatment covered for one month.Before and after treatment,we observed the scores of traditional Chinese medicine symptoms,indicators of renal function[serum creatinine(Scr),blood urea nitrogen(BUN),microalbuminuria(MALB)],indicators of glucose metabolism[fasting plasma glucose(FPG),2-hour postprandial blood glucose(2hPG),glycosylated hemoglobin(HbAlc)],indicators of hemorheology[plasma viscosity(PV),platelet aggregation rate(PAR),fibrinogen(FIB)],Cystatin-C(Cys-C),C-reactive protein(CRP)in the two groups.Results:After treatment,the clinical effect of the observation group was significantly better than the control group(P<0.05).The scores of traditional Chinese medicine symptoms,indicators of renal function(Scr、BUN、UAER),indicators of glucose metabolism(FPG、2hPG、HbAlc),indicators of hemorheology(PV、PAR、FIB),Cys-C and CRP in the two groups were decreased significantly compared with those before treatment(P<0.05),and the decrease in the observation group was superior to that in the control group(P<0.05).Conclusion:Compound of warming yang,descending turbidity and dredging collaterals has remarkable efficacy in treating of diabetic kidney disease patients with yin-yang deficiency and blood stasis syndrome by alleviating clinical symptoms,glucose metabolism,renal function and microcirculatory disturbance,and the mechanism related to alleviation of microinflammation.展开更多
The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models we...The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".展开更多
目的评价桑丹通络颗粒治疗阴虚血瘀证缺血性脑卒中恢复期的临床有效性与安全性。方法采用回顾性队列研究方法,通过湖南省中医药研究院附属医院门诊与住院病历系统收集2021年7月至2022年6月收治的缺血性脑卒中恢复期患者临床资料,选择应...目的评价桑丹通络颗粒治疗阴虚血瘀证缺血性脑卒中恢复期的临床有效性与安全性。方法采用回顾性队列研究方法,通过湖南省中医药研究院附属医院门诊与住院病历系统收集2021年7月至2022年6月收治的缺血性脑卒中恢复期患者临床资料,选择应用桑丹通络颗粒联合西医常规治疗的51例患者为暴露组,匹配仅应用西医常规治疗的102例患者为对照组,两组均治疗2周。比较治疗前后总有效率、美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)评分、日常生活活动能力(activity of daily living,ADL)评分、血脂水平、血液流变学指标和症状总积分等,并对所有患者治疗期间不良反应事件进行记录。结果暴露组临床总有效率84.31%,高于对照组的67.65%(P<0.05)。与治疗前比较,两组治疗后NIHSS、TC、LDL降低(P<0.01或P<0.05),且暴露组低于对照组(P<0.05);两组ADL升高(P<0.01),且暴露组高于对照组(P<0.01)。与治疗前比较,暴露组治疗后症状总积分、血液流变学各项指标降低(P<0.01或P<0.05),且暴露组低于对照组(P<0.01或P<0.05);HDL-C升高(P<0.01),且暴露组高于对照组(P<0.01)。结论桑丹通络颗粒联合西医常规治疗可明显改善阴虚血瘀证缺血性脑卒中恢复期患者症状,降低其神经缺损程度,改善血脂水平和血液流变学指标,有效提高其生存质量,疗效确切且安全稳定。因此,推测桑丹通络颗粒具有一定的临床应用与推广价值。展开更多
目的:探讨中医护理联合认知疗法对气虚血瘀型卒中后认知功能障碍(post-stroke cognitive impairment,PSCI)的影响。方法:将PSCI患者100例采用随机数字表法分为对照组和观察组各50例,对照组给予认知疗法,观察组在对照组基础上增加中医护...目的:探讨中医护理联合认知疗法对气虚血瘀型卒中后认知功能障碍(post-stroke cognitive impairment,PSCI)的影响。方法:将PSCI患者100例采用随机数字表法分为对照组和观察组各50例,对照组给予认知疗法,观察组在对照组基础上增加中医护理。观察两组患者治疗前后神经功能缺损程度、日常生活能力、认知功能障碍程度和中医症状评分及临床疗效和不良反应。结果:1)治疗后两组患者美国国立卫生研究院卒中量表(national institute of health stroke scale,NIHSS)和日常生活活动能力量表(activity of daily living,ADL)评分较治疗前下降,蒙特利尔认知评估量表(Montreal cognitive assessment,MoCA)和简易精神状态检查量表(mini-mental state examination,MMSE)评分较治疗前升高,且观察组各评分改善均优于对照组(P<0.05);2)治疗后两组患者中医各症状评分较治疗前下降,观察组低于对照组(P<0.05);3)观察组总有效率为95.92%,高于对照组的82.98%(P<0.05);4)两组患者治疗期间均无严重不良反应发生。结论:中医护理联合认知疗法对改善气虚血瘀型PSCI疗效更好,安全性较高。展开更多
基金supported by Clinical observation on the treatment of diabetic peripheral neuropathy by supplementing qi,promoting blood circulation and tonifying kidney (grant mumber YJ202324).
文摘Background:Using network pharmacology to explore the potential molecular mechanism of traditional Chinese medicine in treating polycystic ovary syndrome(PCOS)with kidney deficiency and blood stasis syndrome.Method:Collect the related literature materials of PCOS with kidney deficiency and blood stasis syndrome treated by traditional Chinese medicine in four databases in recent ten years,extract the information of prescriptions and complete the frequency analysis.Traditional Chinese Medicine Systems Pharmacology Database was used to screen out the effective components.Use Online Mendelian Inheritance in Man and other databases to screen PCOS disease targets.The intersection targets obtained by clustering prescription and PCOS disease targets were submitted to STRING database for protein-protein interaction network analysis,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes pathways were analysed by Metascape.Result:There are 155 kinds of traditional Chinese medicines used in the literature.The most commonly utilized ones are Cuscutae Semen,Angelicae Sinensis Radix,and Rehmanniae Radix Praeparata.The results of the cluster analysis indicated that the plants most commonly found throughout the prescription were Leonuri Herba,Lycopi Herba,Dipsaci Radix,etc.GO results show that biological processes include cell reaction to organic nitrogen compounds and cell reaction to nitrogen compounds.The functional display of GO molecule includes cytokine receptor binding,signal receptor regulator activity and so on.Kyoto Encyclopedia of Genes and Genomes results show that the possible mechanisms of action are cancer pathway,an endocrine resistance signal pathway.Conclusion:Through data mining,the cluster prescription for PCOS with kidney deficiency and blood stasis syndrome is Leonuri Herba,Lycopi Herba,Dipsaci Radix,etc.The network pharmacology research of cluster prescription shows that the main drug components for treating PCOS with kidney deficiency and blood stasis syndrome are quercetin,kaempferol,luteolin,tanshinone IIA,etc.,which act on PTGS2,NCOA2,and other targets,and treat PCOS with kidney deficiency and blood stasis syndrome through cancer and endocrine resistance.
基金Key R&D Project of Liaoning Provincial Science and Technology Department(No.2018225042)
文摘Objective:To observe the clinical efficacy of compound of owarming yang,descending turbidity and dredging collaterals in the treatment of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome.Methods:Seventy-six patients of diabetic kidney disease with yin-yang deficiency and blood stasis syndrome were randomly divided into observation group and control group,thirty-eight cases in each group.The control group was given conventional western medicine treatment,while the observation group took compound of owarming yang,descending turbidity and dredging collaterals orally on the basis of conventional western medicine treatment.The course of treatment covered for one month.Before and after treatment,we observed the scores of traditional Chinese medicine symptoms,indicators of renal function[serum creatinine(Scr),blood urea nitrogen(BUN),microalbuminuria(MALB)],indicators of glucose metabolism[fasting plasma glucose(FPG),2-hour postprandial blood glucose(2hPG),glycosylated hemoglobin(HbAlc)],indicators of hemorheology[plasma viscosity(PV),platelet aggregation rate(PAR),fibrinogen(FIB)],Cystatin-C(Cys-C),C-reactive protein(CRP)in the two groups.Results:After treatment,the clinical effect of the observation group was significantly better than the control group(P<0.05).The scores of traditional Chinese medicine symptoms,indicators of renal function(Scr、BUN、UAER),indicators of glucose metabolism(FPG、2hPG、HbAlc),indicators of hemorheology(PV、PAR、FIB),Cys-C and CRP in the two groups were decreased significantly compared with those before treatment(P<0.05),and the decrease in the observation group was superior to that in the control group(P<0.05).Conclusion:Compound of warming yang,descending turbidity and dredging collaterals has remarkable efficacy in treating of diabetic kidney disease patients with yin-yang deficiency and blood stasis syndrome by alleviating clinical symptoms,glucose metabolism,renal function and microcirculatory disturbance,and the mechanism related to alleviation of microinflammation.
基金Supported by the National Natural Science Foundation of China (Grant Nos 30630076 and 30572228)the National 11th 5 Year Technology Support Program (Grant No 2006BAI08B04-05)
文摘The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".
文摘目的评价桑丹通络颗粒治疗阴虚血瘀证缺血性脑卒中恢复期的临床有效性与安全性。方法采用回顾性队列研究方法,通过湖南省中医药研究院附属医院门诊与住院病历系统收集2021年7月至2022年6月收治的缺血性脑卒中恢复期患者临床资料,选择应用桑丹通络颗粒联合西医常规治疗的51例患者为暴露组,匹配仅应用西医常规治疗的102例患者为对照组,两组均治疗2周。比较治疗前后总有效率、美国国立卫生研究院卒中量表(National Institute of Health Stroke Scale,NIHSS)评分、日常生活活动能力(activity of daily living,ADL)评分、血脂水平、血液流变学指标和症状总积分等,并对所有患者治疗期间不良反应事件进行记录。结果暴露组临床总有效率84.31%,高于对照组的67.65%(P<0.05)。与治疗前比较,两组治疗后NIHSS、TC、LDL降低(P<0.01或P<0.05),且暴露组低于对照组(P<0.05);两组ADL升高(P<0.01),且暴露组高于对照组(P<0.01)。与治疗前比较,暴露组治疗后症状总积分、血液流变学各项指标降低(P<0.01或P<0.05),且暴露组低于对照组(P<0.01或P<0.05);HDL-C升高(P<0.01),且暴露组高于对照组(P<0.01)。结论桑丹通络颗粒联合西医常规治疗可明显改善阴虚血瘀证缺血性脑卒中恢复期患者症状,降低其神经缺损程度,改善血脂水平和血液流变学指标,有效提高其生存质量,疗效确切且安全稳定。因此,推测桑丹通络颗粒具有一定的临床应用与推广价值。
文摘目的:探讨中医护理联合认知疗法对气虚血瘀型卒中后认知功能障碍(post-stroke cognitive impairment,PSCI)的影响。方法:将PSCI患者100例采用随机数字表法分为对照组和观察组各50例,对照组给予认知疗法,观察组在对照组基础上增加中医护理。观察两组患者治疗前后神经功能缺损程度、日常生活能力、认知功能障碍程度和中医症状评分及临床疗效和不良反应。结果:1)治疗后两组患者美国国立卫生研究院卒中量表(national institute of health stroke scale,NIHSS)和日常生活活动能力量表(activity of daily living,ADL)评分较治疗前下降,蒙特利尔认知评估量表(Montreal cognitive assessment,MoCA)和简易精神状态检查量表(mini-mental state examination,MMSE)评分较治疗前升高,且观察组各评分改善均优于对照组(P<0.05);2)治疗后两组患者中医各症状评分较治疗前下降,观察组低于对照组(P<0.05);3)观察组总有效率为95.92%,高于对照组的82.98%(P<0.05);4)两组患者治疗期间均无严重不良反应发生。结论:中医护理联合认知疗法对改善气虚血瘀型PSCI疗效更好,安全性较高。
