Let and let be the set of four ribbon L-shaped n-ominoes. We study tiling problems for regions in a square lattice by . Our main result shows a remarkable property of this set of tiles: any tiling of the first quadran...Let and let be the set of four ribbon L-shaped n-ominoes. We study tiling problems for regions in a square lattice by . Our main result shows a remarkable property of this set of tiles: any tiling of the first quadrant by , n even, reduces to a tiling by and rectangles, each rectangle being covered by two ribbon L-shaped n-ominoes. An application of our result is the characterization of all rectangles that can be tiled by , n even: a rectangle can be tiled by , n even, if and only if both of its sides are even and at least one side is divisible by n. Another application is the existence of the local move property for an infinite family of sets of tiles: , n even, has the local move property for the class of rectangular regions with respect to the local moves that interchange a tiling of an square by n/2 vertical rectangles, with a tiling by n/2 horizontal rectangles, each vertical/horizontal rectangle being covered by two ribbon L-shaped n-ominoes. We show that none of these results are valid for any odd n. The rectangular pattern of a tiling of the first quadrant persists if we add an extra tile to , n even. A rectangle can be tiled by the larger set of tiles if and only if it has both sides even. We also show that our main result implies that a skewed L-shaped n-omino, n even, is not a replicating tile of order k2 for any odd k.展开更多
The problem of tiling rectangles by polyominoes generated large interest. A related one is the problem of tiling parallelograms by twisted polyominoes. Both problems are related with tilings of (skewed) quadrants by p...The problem of tiling rectangles by polyominoes generated large interest. A related one is the problem of tiling parallelograms by twisted polyominoes. Both problems are related with tilings of (skewed) quadrants by polyominoes. Indeed, if all tilings of a (skewed) quadrant by a tile set can be reduced to a tiling by congruent rectangles (parallelograms), this provides information about tilings of rectangles (parallelograms). We consider a class of tile sets in a square lattice appearing from arbitrary dissections of rectangles in two L-shaped polyominoes and from symmetries of these tiles about the first bisector. Only translations of the tiles are allowed in a tiling. If the sides of the dissected rectangle are coprime, we show the existence of tilings of all (skewed) quadrants that do not follow the rectangular (parallelogram) pattern. If one of the sides of the dissected rectangle is 2 and the other is odd, we also show tilings of rectangles by the tile set that do not follow the rectangular pattern. If one of the sides of the dissected rectangle is 2 and the other side is even, we show a new infinite family of tile sets that follows the rectangular pattern when tiling one of the quadrants. For this type of dis-section, we also show a new infinite family that does not follow the rectangular pattern when tiling rectangles. Finally, we investigate more general dissections of rectangles, with. Here we show infinite families of tile sets that follow the rectangular pattern for a quadrant and infinite families that do not follow the rectangular pattern for any quadrant. We also show, for infinite families of tile sets of this type, tilings of rectangles that do not follow the rectangular pattern.展开更多
Resveratrol synthase (RS) is a key enzyme that plays a critical role in the resveratrol synthesis pathway. In this study, six RS genes were isolated and characterized from peanut variety “Zhenzhu Hong” by silico clo...Resveratrol synthase (RS) is a key enzyme that plays a critical role in the resveratrol synthesis pathway. In this study, six RS genes were isolated and characterized from peanut variety “Zhenzhu Hong” by silico cloning and RT-PCR. Bioinformatics analysis showed that deduced amino acid sequences of the six cloned RS genes were highly conserved with a similarity from 95% to 99% when compared to the RS genes which had been deposited at the GenBank. The results of amino acid sequences analysis showed six RS proteins contained the Chal_Sti_Synt_N and ACP_Syn_III_C domains and can be classified to same family but with different evolutionary distance. Expression pattern analysis by QRT-PCR provided evidence indicating that the mRNA of six RS genes were primarily expressed in the peanut shell at different developmental stages with different expression levels, but only lower levels of them were evident in the peanut kernel. The subcellular localization of RS protein in onion epidermal cell was performed by Agrobacterium tumefaciens-mediated transformation and the green fluorescent was monitored by confocal fluorescence microscopy. The results indicated that, RS1 and RS5 were located in the nucleus and plasma membrane respectively, while the RS2, RS3, RS4 and RS6 were located in both nucleus inner membrane and plasma membrane. The data will provide basic information for elucidating the regulatory mechanisms and enzyme kinetics underlying the RS genes in the resveratrol synthase pathway.展开更多
In the network security system,intrusion detection plays a significant role.The network security system detects the malicious actions in the network and also conforms the availability,integrity and confidentiality of da...In the network security system,intrusion detection plays a significant role.The network security system detects the malicious actions in the network and also conforms the availability,integrity and confidentiality of data informa-tion resources.Intrusion identification system can easily detect the false positive alerts.If large number of false positive alerts are created then it makes intrusion detection system as difficult to differentiate the false positive alerts from genuine attacks.Many research works have been done.The issues in the existing algo-rithms are more memory space and need more time to execute the transactions of records.This paper proposes a novel framework of network security Intrusion Detection System(IDS)using Modified Frequent Pattern(MFP-Tree)via K-means algorithm.The accuracy rate of Modified Frequent Pattern Tree(MFPT)-K means method infinding the various attacks are Normal 94.89%,for DoS based attack 98.34%,for User to Root(U2R)attacks got 96.73%,Remote to Local(R2L)got 95.89%and Probe attack got 92.67%and is optimal when it is compared with other existing algorithms of K-Means and APRIORI.展开更多
The soft/hard composite patterned media have potential to be the next generation of magnetic recording, but the composing modes of soft and hard materials have not been investigated systematically. L10 FePt-based soft...The soft/hard composite patterned media have potential to be the next generation of magnetic recording, but the composing modes of soft and hard materials have not been investigated systematically. L10 FePt-based soft/hard composite patterned media with an anisotropic constant distribution are studied by micromagnetic simulation. Square arrays and hexagonal arrays with various pitch sizes are simulated for two composing types: the soft layer that encloses the hard dots and the soft layer that covers the whole surface. It is found that the soft material can reduce the switching fields of bits effectively for all models. Compared with the first type, the second type of models possess low switching fields, narrow switching field distributions, and high gain factors due to the introduction of inter-bit exchange coupling. Furthermore, the readout waveforms of the second type are not deteriorated by the inter-bit soft layers. Since the recording density of hexagonal arrays are higher than that of square arrays with the same center-to-center distances, the readout waveforrns of hexagonal arrays are a little worse, although other simulation results are similar for these two arrays.展开更多
Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological re...Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological responses to biotic or abiotic stresses.It plays a key role in distributing carbohydrates and sugar signal transporting.In the present study,eight SnRK1 coding genes were identified in sorghum(Sorghum bicolor L.)via sequences alignment,with three forαsubunits(SnRK1α1 to SnRK1α3),three forβ(SnRK1β1 to SnRK1β3),and one for bothγ(SnRK1γ)andβγ(SnRK1βγ).These eight corresponding genes located on five chromosomes(Chr)of Chr1–3,Chr7,and Chr9 and presented collinearities to SnRK1s from maize and rice,exhibiting highly conserved domains within the same subunits from the three kinds of cereals.Expression results via qRT-PCR showed that different coding genes of SnRK1s in sorghum possessed similar expression patterns except for SnRK1α3 with a low expression level in grains and SnRK1β2 with a relatively high expression level in inflorescences.Results of subcellular localization in sorghum leaf protoplast showed that SnRK1α1/α2/α3/γmainly located on organelles,while the rest four of SnRK1β1/β2/β3/βγlocated on both membranes and some organelles.Besides,three combinations were discovered among eight SnRK1 subunits in sorghum through yeast two hybrid,includingα1-β2-βγ,α2-β3-γ,andα3-β3-γ.These results provide informative references for the following functional dissection of SnRK1 subunits in sorghum.展开更多
Each type of fishery has its own characteristic behavior, and understanding this condition is an important part of devel- oping and managing fishing operations comprehensively. Based on the random walk model, the rela...Each type of fishery has its own characteristic behavior, and understanding this condition is an important part of devel- oping and managing fishing operations comprehensively. Based on the random walk model, the relationship between distance and frequency distribution of adjacent fishing positions was analyzed by the Commission for Conservation of Antarctic Marine Living Resources (CCAMLR) Subareas 48.1, 48.2, and 48.3. The frequency of distances between consecutive hauls demonstrated a heavy- tailed distribution, which could be used to estimate the value of parameter μ in the power function F(l)~ l μ of a random walk model to determine the type of random walk patterns that characterize Chinese krill fishery. Results indicated that the fishing pattern of the Chinese krill fishery is consistent with the Lévy random walk model, with which the step-length is applied to analyze the walking pattern. When a defined walk in a space of dimension is greater than one, the steps made are in isotropic random directions. Fur- thermore, a strong and positive correlation between fishing behavior (using the parameter μ as an indicator) and catch per unit effort of the Chinese krill fishery was observed.展开更多
The maximum of k numerical functions defined on , , by , ??is used here in Statistical classification. Previously, it has been used in Statistical Discrimination [1] and in Clustering [2]. We present first some theore...The maximum of k numerical functions defined on , , by , ??is used here in Statistical classification. Previously, it has been used in Statistical Discrimination [1] and in Clustering [2]. We present first some theoretical results on this function, and then its application in classification using a computer program we have developed. This approach leads to clear decisions, even in cases where the extension to several classes of Fisher’s linear discriminant function fails to be effective.展开更多
Estimation of the transpiration rate for a tree is generally based on sap flow measurements within the hydro-active stem xylem. In this study, radial variation of sap flow velocity(Js) was investigated at five depth...Estimation of the transpiration rate for a tree is generally based on sap flow measurements within the hydro-active stem xylem. In this study, radial variation of sap flow velocity(Js) was investigated at five depths of the xylem(1, 2, 3, 5 and 8 cm under the cambium) in three mature Xinjiang poplar(Populus alba L. var. pyramidalis) trees grown at the Gansu Minqin National Studies Station for Desert Steppe Ecosystem from May to October 2011. Thermal dissipation probes of various lengths manufactured according to the Granier's design were installed into each tree for simultaneous observation of the radial patterns of Js through the xylem. The radial patterns were found to fit the four-parameter GaussAmp equation. The peak Js was about 27.02±0.95 kg/(dm2?d) at approximately 3 to 5 cm deep from the cambium of the three trees,and the lowest Js appeared at 1 cm deep in most of the time. Approximately 50% of the total sap flow in Xinjiang poplar occurred within one-third of the xylem from its outer radius, whereas 90% of the total sap flow occurred within two-fifth of the xylem. In addition, the innermost point of the xylem(at 8-cm depth), which appeared as the penultimate sap flow in most cases during the study period, was hydro-active with Js,8 of 7.55±3.83 kg/(dm2?d). The radial pattern of Js was found to be steeper in midday than in other time of the day, and steeper diurnal fluctuations were recorded in June, July and August(the mid-growing season). Maximum differences between the lowest Js(Js,1 or Js,8) and the highest Js(Js,3 or Js,5) from May through October were 12.41, 17.35, 16.30, 18.52, 12.60 and 16.04 g/(cm2?h), respectively. The time-dependent changes of Js along the radial profile(except at 1-cm depth) were strongly related to the reference evapotranspiration(ET0). Due to significant radial variability of Js, the mean daily sap flow at the whole-tree level could be over-estimated by up to 29.69% when only a single probe at depth of 2 cm was used. However, the accuracy of the estimation of sap flow in Xinjiang poplar could be significantly improved using a correction coefficient of 0.885.展开更多
Human Immunodeficiency Virus Type 1 exists in vivo as quasispecies, and one of the genome's characteristics is its diversity. During the antiretroviral therapy, drug resistance is the main obstacle to effective vi...Human Immunodeficiency Virus Type 1 exists in vivo as quasispecies, and one of the genome's characteristics is its diversity. During the antiretroviral therapy, drug resistance is the main obstacle to effective viral prevention. Understanding the molecular evolution process is fundamental to analyze the mechanism of drug resistance and develop a strategy to minimize resistance. Objective: The molecular evolution of drug resistance of one patient who had received reverse transcriptase inhibitors for a long time and had treatment which replaced Nevirapine with Indinavir was analyzed, with the aim of observing the drug resistance evolution pathway. Methods: The patient, XLF, was followed-up for six successive times. The viral populations were amplified and sequenced by single-genome amplification. All the sequences were submitted to the Stanford HIV Drug Resistance Database for the analysis of genotypic drug resistance. Results: 149 entire protease and 171 entire reverse transcriptase sequences were obtained from these samples, and all sequences were identified as subtype B. Before the patient received Indinavir, the viral population only had some polymorphisms in the protease sequences. After the patient began Indinavir treatment, the variants carrying polymorphisms declined while variants carrying the secondary mutation G73S gained the advantage. As therapy was prolonged, G73S was combined with M46I/L90M to form a resistance pattern M46I/G73S/L90M, which then became the dominant population. 97.9% of variants had the M46I/G73S/L90M pattern at XLF6. During the emergence of protease inhibitors resistance, reverse transcriptase inhibitors resistance maintained high levels. Conclusion: Indinavirresistance evolution was observed by single-genome amplification. During the course of changing the regimen to incorporate Indinavir, the G73S mutation occurred and was combined with M46I/L90M.展开更多
Based on the new finding of Wenchang L low-resistivity light oil field, the finding process, reservoir charac- teristics and pool-forming pattern were studied. The oil-rock correlation, neritic reservoir type, hydroca...Based on the new finding of Wenchang L low-resistivity light oil field, the finding process, reservoir charac- teristics and pool-forming pattern were studied. The oil-rock correlation, neritic reservoir type, hydrocarbon conduct system and dominant migration and accumulation direction, and new techniques were discussed. The results showed that large amount of hydrocarbon generated by shallow lacustrine mudstone and shale of Eocene Wenchang formation could migrate from sag to Qionghai uplift distantly; neritic shoal-bar reservoir have developed in the 1 st member of Zhujiang formation, dip-sag faults and regional good marine sandstone layers of the 1 st and the 2nd member of Zhujiang formation have constituted hydrocarbon conduct system in Qionghai uplift ; the late fault system which were controlled by Dongsha tectonic movement constructed the connection between lower hydrocarbon and upper neritic shoal-bar reservoir. There- fore, the pool-forming pattern with "vertical migration and accumulation, secondary strncture-lithology-dominated" was proposed. Finally the enlightenment of exploratin~ new findings was discussed.展开更多
文摘Let and let be the set of four ribbon L-shaped n-ominoes. We study tiling problems for regions in a square lattice by . Our main result shows a remarkable property of this set of tiles: any tiling of the first quadrant by , n even, reduces to a tiling by and rectangles, each rectangle being covered by two ribbon L-shaped n-ominoes. An application of our result is the characterization of all rectangles that can be tiled by , n even: a rectangle can be tiled by , n even, if and only if both of its sides are even and at least one side is divisible by n. Another application is the existence of the local move property for an infinite family of sets of tiles: , n even, has the local move property for the class of rectangular regions with respect to the local moves that interchange a tiling of an square by n/2 vertical rectangles, with a tiling by n/2 horizontal rectangles, each vertical/horizontal rectangle being covered by two ribbon L-shaped n-ominoes. We show that none of these results are valid for any odd n. The rectangular pattern of a tiling of the first quadrant persists if we add an extra tile to , n even. A rectangle can be tiled by the larger set of tiles if and only if it has both sides even. We also show that our main result implies that a skewed L-shaped n-omino, n even, is not a replicating tile of order k2 for any odd k.
文摘The problem of tiling rectangles by polyominoes generated large interest. A related one is the problem of tiling parallelograms by twisted polyominoes. Both problems are related with tilings of (skewed) quadrants by polyominoes. Indeed, if all tilings of a (skewed) quadrant by a tile set can be reduced to a tiling by congruent rectangles (parallelograms), this provides information about tilings of rectangles (parallelograms). We consider a class of tile sets in a square lattice appearing from arbitrary dissections of rectangles in two L-shaped polyominoes and from symmetries of these tiles about the first bisector. Only translations of the tiles are allowed in a tiling. If the sides of the dissected rectangle are coprime, we show the existence of tilings of all (skewed) quadrants that do not follow the rectangular (parallelogram) pattern. If one of the sides of the dissected rectangle is 2 and the other is odd, we also show tilings of rectangles by the tile set that do not follow the rectangular pattern. If one of the sides of the dissected rectangle is 2 and the other side is even, we show a new infinite family of tile sets that follows the rectangular pattern when tiling one of the quadrants. For this type of dis-section, we also show a new infinite family that does not follow the rectangular pattern when tiling rectangles. Finally, we investigate more general dissections of rectangles, with. Here we show infinite families of tile sets that follow the rectangular pattern for a quadrant and infinite families that do not follow the rectangular pattern for any quadrant. We also show, for infinite families of tile sets of this type, tilings of rectangles that do not follow the rectangular pattern.
文摘Resveratrol synthase (RS) is a key enzyme that plays a critical role in the resveratrol synthesis pathway. In this study, six RS genes were isolated and characterized from peanut variety “Zhenzhu Hong” by silico cloning and RT-PCR. Bioinformatics analysis showed that deduced amino acid sequences of the six cloned RS genes were highly conserved with a similarity from 95% to 99% when compared to the RS genes which had been deposited at the GenBank. The results of amino acid sequences analysis showed six RS proteins contained the Chal_Sti_Synt_N and ACP_Syn_III_C domains and can be classified to same family but with different evolutionary distance. Expression pattern analysis by QRT-PCR provided evidence indicating that the mRNA of six RS genes were primarily expressed in the peanut shell at different developmental stages with different expression levels, but only lower levels of them were evident in the peanut kernel. The subcellular localization of RS protein in onion epidermal cell was performed by Agrobacterium tumefaciens-mediated transformation and the green fluorescent was monitored by confocal fluorescence microscopy. The results indicated that, RS1 and RS5 were located in the nucleus and plasma membrane respectively, while the RS2, RS3, RS4 and RS6 were located in both nucleus inner membrane and plasma membrane. The data will provide basic information for elucidating the regulatory mechanisms and enzyme kinetics underlying the RS genes in the resveratrol synthase pathway.
文摘In the network security system,intrusion detection plays a significant role.The network security system detects the malicious actions in the network and also conforms the availability,integrity and confidentiality of data informa-tion resources.Intrusion identification system can easily detect the false positive alerts.If large number of false positive alerts are created then it makes intrusion detection system as difficult to differentiate the false positive alerts from genuine attacks.Many research works have been done.The issues in the existing algo-rithms are more memory space and need more time to execute the transactions of records.This paper proposes a novel framework of network security Intrusion Detection System(IDS)using Modified Frequent Pattern(MFP-Tree)via K-means algorithm.The accuracy rate of Modified Frequent Pattern Tree(MFPT)-K means method infinding the various attacks are Normal 94.89%,for DoS based attack 98.34%,for User to Root(U2R)attacks got 96.73%,Remote to Local(R2L)got 95.89%and Probe attack got 92.67%and is optimal when it is compared with other existing algorithms of K-Means and APRIORI.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.51171086 and 61272076)the Young Scientists Fund of the National Natural Science Foundation of China(Grant No.61003041)
文摘The soft/hard composite patterned media have potential to be the next generation of magnetic recording, but the composing modes of soft and hard materials have not been investigated systematically. L10 FePt-based soft/hard composite patterned media with an anisotropic constant distribution are studied by micromagnetic simulation. Square arrays and hexagonal arrays with various pitch sizes are simulated for two composing types: the soft layer that encloses the hard dots and the soft layer that covers the whole surface. It is found that the soft material can reduce the switching fields of bits effectively for all models. Compared with the first type, the second type of models possess low switching fields, narrow switching field distributions, and high gain factors due to the introduction of inter-bit exchange coupling. Furthermore, the readout waveforms of the second type are not deteriorated by the inter-bit soft layers. Since the recording density of hexagonal arrays are higher than that of square arrays with the same center-to-center distances, the readout waveforrns of hexagonal arrays are a little worse, although other simulation results are similar for these two arrays.
基金supported by the National Natural Science Foundation of China(32001607)the Fundamental Research Funds for the Central Universities of Southwest University,China(SWU118087)。
文摘Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein kinases.It commonly mediates plant growth and development,cross-talks with metabolism processes and physiological responses to biotic or abiotic stresses.It plays a key role in distributing carbohydrates and sugar signal transporting.In the present study,eight SnRK1 coding genes were identified in sorghum(Sorghum bicolor L.)via sequences alignment,with three forαsubunits(SnRK1α1 to SnRK1α3),three forβ(SnRK1β1 to SnRK1β3),and one for bothγ(SnRK1γ)andβγ(SnRK1βγ).These eight corresponding genes located on five chromosomes(Chr)of Chr1–3,Chr7,and Chr9 and presented collinearities to SnRK1s from maize and rice,exhibiting highly conserved domains within the same subunits from the three kinds of cereals.Expression results via qRT-PCR showed that different coding genes of SnRK1s in sorghum possessed similar expression patterns except for SnRK1α3 with a low expression level in grains and SnRK1β2 with a relatively high expression level in inflorescences.Results of subcellular localization in sorghum leaf protoplast showed that SnRK1α1/α2/α3/γmainly located on organelles,while the rest four of SnRK1β1/β2/β3/βγlocated on both membranes and some organelles.Besides,three combinations were discovered among eight SnRK1 subunits in sorghum through yeast two hybrid,includingα1-β2-βγ,α2-β3-γ,andα3-β3-γ.These results provide informative references for the following functional dissection of SnRK1 subunits in sorghum.
基金sponsored partly by the National Key R&D Program of China (No. 2018YFC1406801)the National Natural Science Foundation of China (No. 41776185)the Key Course Construction Program of the Shanghai Municipal Education Commission (Biological Oceanography) (No. A1-0201-00-1204)
文摘Each type of fishery has its own characteristic behavior, and understanding this condition is an important part of devel- oping and managing fishing operations comprehensively. Based on the random walk model, the relationship between distance and frequency distribution of adjacent fishing positions was analyzed by the Commission for Conservation of Antarctic Marine Living Resources (CCAMLR) Subareas 48.1, 48.2, and 48.3. The frequency of distances between consecutive hauls demonstrated a heavy- tailed distribution, which could be used to estimate the value of parameter μ in the power function F(l)~ l μ of a random walk model to determine the type of random walk patterns that characterize Chinese krill fishery. Results indicated that the fishing pattern of the Chinese krill fishery is consistent with the Lévy random walk model, with which the step-length is applied to analyze the walking pattern. When a defined walk in a space of dimension is greater than one, the steps made are in isotropic random directions. Fur- thermore, a strong and positive correlation between fishing behavior (using the parameter μ as an indicator) and catch per unit effort of the Chinese krill fishery was observed.
文摘The maximum of k numerical functions defined on , , by , ??is used here in Statistical classification. Previously, it has been used in Statistical Discrimination [1] and in Clustering [2]. We present first some theoretical results on this function, and then its application in classification using a computer program we have developed. This approach leads to clear decisions, even in cases where the extension to several classes of Fisher’s linear discriminant function fails to be effective.
基金supported by the National Natural Science Foundation of China (31070628)Field support for this research was provided by Gansu Minqin National Studies Station for Desert Steppe Ecosystem
文摘Estimation of the transpiration rate for a tree is generally based on sap flow measurements within the hydro-active stem xylem. In this study, radial variation of sap flow velocity(Js) was investigated at five depths of the xylem(1, 2, 3, 5 and 8 cm under the cambium) in three mature Xinjiang poplar(Populus alba L. var. pyramidalis) trees grown at the Gansu Minqin National Studies Station for Desert Steppe Ecosystem from May to October 2011. Thermal dissipation probes of various lengths manufactured according to the Granier's design were installed into each tree for simultaneous observation of the radial patterns of Js through the xylem. The radial patterns were found to fit the four-parameter GaussAmp equation. The peak Js was about 27.02±0.95 kg/(dm2?d) at approximately 3 to 5 cm deep from the cambium of the three trees,and the lowest Js appeared at 1 cm deep in most of the time. Approximately 50% of the total sap flow in Xinjiang poplar occurred within one-third of the xylem from its outer radius, whereas 90% of the total sap flow occurred within two-fifth of the xylem. In addition, the innermost point of the xylem(at 8-cm depth), which appeared as the penultimate sap flow in most cases during the study period, was hydro-active with Js,8 of 7.55±3.83 kg/(dm2?d). The radial pattern of Js was found to be steeper in midday than in other time of the day, and steeper diurnal fluctuations were recorded in June, July and August(the mid-growing season). Maximum differences between the lowest Js(Js,1 or Js,8) and the highest Js(Js,3 or Js,5) from May through October were 12.41, 17.35, 16.30, 18.52, 12.60 and 16.04 g/(cm2?h), respectively. The time-dependent changes of Js along the radial profile(except at 1-cm depth) were strongly related to the reference evapotranspiration(ET0). Due to significant radial variability of Js, the mean daily sap flow at the whole-tree level could be over-estimated by up to 29.69% when only a single probe at depth of 2 cm was used. However, the accuracy of the estimation of sap flow in Xinjiang poplar could be significantly improved using a correction coefficient of 0.885.
基金National Natural Science Foundation of China (30830088 and 30800938)The National Key and Special Projects on Major Infectious Disease Grant (2008 ZX10001-004)
文摘Human Immunodeficiency Virus Type 1 exists in vivo as quasispecies, and one of the genome's characteristics is its diversity. During the antiretroviral therapy, drug resistance is the main obstacle to effective viral prevention. Understanding the molecular evolution process is fundamental to analyze the mechanism of drug resistance and develop a strategy to minimize resistance. Objective: The molecular evolution of drug resistance of one patient who had received reverse transcriptase inhibitors for a long time and had treatment which replaced Nevirapine with Indinavir was analyzed, with the aim of observing the drug resistance evolution pathway. Methods: The patient, XLF, was followed-up for six successive times. The viral populations were amplified and sequenced by single-genome amplification. All the sequences were submitted to the Stanford HIV Drug Resistance Database for the analysis of genotypic drug resistance. Results: 149 entire protease and 171 entire reverse transcriptase sequences were obtained from these samples, and all sequences were identified as subtype B. Before the patient received Indinavir, the viral population only had some polymorphisms in the protease sequences. After the patient began Indinavir treatment, the variants carrying polymorphisms declined while variants carrying the secondary mutation G73S gained the advantage. As therapy was prolonged, G73S was combined with M46I/L90M to form a resistance pattern M46I/G73S/L90M, which then became the dominant population. 97.9% of variants had the M46I/G73S/L90M pattern at XLF6. During the emergence of protease inhibitors resistance, reverse transcriptase inhibitors resistance maintained high levels. Conclusion: Indinavirresistance evolution was observed by single-genome amplification. During the course of changing the regimen to incorporate Indinavir, the G73S mutation occurred and was combined with M46I/L90M.
文摘Based on the new finding of Wenchang L low-resistivity light oil field, the finding process, reservoir charac- teristics and pool-forming pattern were studied. The oil-rock correlation, neritic reservoir type, hydrocarbon conduct system and dominant migration and accumulation direction, and new techniques were discussed. The results showed that large amount of hydrocarbon generated by shallow lacustrine mudstone and shale of Eocene Wenchang formation could migrate from sag to Qionghai uplift distantly; neritic shoal-bar reservoir have developed in the 1 st member of Zhujiang formation, dip-sag faults and regional good marine sandstone layers of the 1 st and the 2nd member of Zhujiang formation have constituted hydrocarbon conduct system in Qionghai uplift ; the late fault system which were controlled by Dongsha tectonic movement constructed the connection between lower hydrocarbon and upper neritic shoal-bar reservoir. There- fore, the pool-forming pattern with "vertical migration and accumulation, secondary strncture-lithology-dominated" was proposed. Finally the enlightenment of exploratin~ new findings was discussed.