The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McA...The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McAb 3H11. The antibody was reduced at room temperature for 30 min with a 1000-fold molar ratio excess of 2mercaptoethanol. The mean labeling efficiency of ￣(99m)Tc3H11 was more than 95%. The immunoreactivity of ￣(99m)Tc-3H11 was more than 80% by ELISA's method. Competition results in vitro showed that ￣(99m)Tc was combined with the high affinity sites of antibody by HPLC. The biodistribution in nude mice bearing 823 gastric cancer xenographts showed that the radioactivity of tumor uptake at 24 h post-injection was the highest except for kidney.The tumor uptake was 8.98±2.42% i.d/g.The count ratio of tumor to blood was over 1.5 and that tumor to liver was more than 2.5 at 24 h post-injection.And the tumor was clearly imaged at 22 h post-injection. The initial clinical results showed that￣(99m)Tc-3H11 was stable in vivo and well good located at the lesion sites.展开更多
The appearance and spread of antibiotic-resistant pathogens known as antimicrobial resistance(AMR)is one of the major worldwide health crises that humanity have to deal with over the next decades.One of the main metho...The appearance and spread of antibiotic-resistant pathogens known as antimicrobial resistance(AMR)is one of the major worldwide health crises that humanity have to deal with over the next decades.One of the main methods for addressing AMR is the effective screening for antimicrobial insensitivity in clinical and environmental monitoring.Current clinical laboratory procedures use traditional culturebased antibiotic susceptibility testing(AST)methods,which can take up to 24 h to identify which drug is suitable for the infection inhibition.Therefore,it is vital to develop novel strategies that offer quick,simple,affordable,reliable,sensitive and accurate AMR monitoring.Sensors for AMR markers detection could possess the essential qualities for quickly identifying resistant microorganisms and could give vital data for the selection of antibacterial drugs administration.This review offers a summary of the innovative application of these AMR markers detection strategies focusing on healthcare and environmental surveillance for the AMR genotypic or phenotypic assessment.展开更多
文摘The Schwartz technique is a simple method for labeling McAb directly with ￣(99m)Tc and can he utilized to design a one-step radiopharmacelltical kit.In this study,it was applied to label anti-human gastric cancer McAb 3H11. The antibody was reduced at room temperature for 30 min with a 1000-fold molar ratio excess of 2mercaptoethanol. The mean labeling efficiency of ￣(99m)Tc3H11 was more than 95%. The immunoreactivity of ￣(99m)Tc-3H11 was more than 80% by ELISA's method. Competition results in vitro showed that ￣(99m)Tc was combined with the high affinity sites of antibody by HPLC. The biodistribution in nude mice bearing 823 gastric cancer xenographts showed that the radioactivity of tumor uptake at 24 h post-injection was the highest except for kidney.The tumor uptake was 8.98±2.42% i.d/g.The count ratio of tumor to blood was over 1.5 and that tumor to liver was more than 2.5 at 24 h post-injection.And the tumor was clearly imaged at 22 h post-injection. The initial clinical results showed that￣(99m)Tc-3H11 was stable in vivo and well good located at the lesion sites.
基金supported by the National Natural Science Foundation of China(No.82202221)the Natural Science Foundation of Anhui Province(No.2208085QB39)College Students’Innovative Entrepreneurial Training Plan Program(No.202110359071).
文摘The appearance and spread of antibiotic-resistant pathogens known as antimicrobial resistance(AMR)is one of the major worldwide health crises that humanity have to deal with over the next decades.One of the main methods for addressing AMR is the effective screening for antimicrobial insensitivity in clinical and environmental monitoring.Current clinical laboratory procedures use traditional culturebased antibiotic susceptibility testing(AST)methods,which can take up to 24 h to identify which drug is suitable for the infection inhibition.Therefore,it is vital to develop novel strategies that offer quick,simple,affordable,reliable,sensitive and accurate AMR monitoring.Sensors for AMR markers detection could possess the essential qualities for quickly identifying resistant microorganisms and could give vital data for the selection of antibacterial drugs administration.This review offers a summary of the innovative application of these AMR markers detection strategies focusing on healthcare and environmental surveillance for the AMR genotypic or phenotypic assessment.
