降解检材STR分型Ladder-Like条带形成原因的探讨

目的探讨降解DNA短串联重复序列PCR扩增产物在PAGE中Ladder-L ike条带的形成原因。方法用一组人工合成的不同长度的D8S1179DNA单链,模拟降解检材中的断裂DNA;利用John M设计的D8S1179引物对模拟模板的DNA单链进行PCR扩增,PAGE分离。结果不同模拟模板的DNA单链的组合可以扩增出不同的Lad-der-L ike条带。结论短串联重复序列Ladder-like条带是PCR过程中降解DNA多态区互补扩增的产物。

Female Struggle in Patriarchal Society-Comparative Interpretation of Gimmal and Crumbs of Agalloch Eaglewood-the First Incense Burner

Zhang Ailin created so many various kinds of female charaters in her novels;females who own distinct characters and identities just have similar fates and struggles.This paper tries to probe into the similar fates and struggles of two heroines with different identities in the same social background by analysing the novel Gimmal and Crumbs of Agalloch Eaglewood-the First Incense Burner from perspective of feminism.

极性蛋白Crumbs胞内域功能保守性研究

跨膜蛋白Crumbs(Crb)是细胞顶部的决定因子,对上皮细胞顶?底极性的建立和维持起着关键的作用。其胞内域虽然仅有37个氨基酸,但对Crb的功能必不可少。在果蝇(Drosophila melanogaster)中,如果胞内域发生突变,将造成胚胎发育异常、上皮细胞顶底极性丧失等严重后果。Crb胞内域从果蝇到小鼠(Mus musculus)和人类(Homo sapiens)具有很高的同源性,但线虫(Caenorhabditis elegans)两个Crb蛋白的胞内域与果蝇和哺乳动物却较为不同。为验证线虫Crb蛋白胞内域是否功能保守,本文利用基因组工程法(Genomic engineering),将果蝇基因组中Crb基因编码胞内域的部分替换为一致性和相似性较远的线虫Crb2基因的相应区段。与其他Crb胞内域突变果蝇不同,替换突变体胚胎发育正常,Crb及其他极性蛋白的表达和定位正常,胚胎上皮细胞顶底极性能够正确的建立和维持。这些结果证实虽然线虫和果蝇Crb蛋白胞内域之间存在大量序列变异,但重要的氨基酸位点和功能模块则完全保守。

SYNTHESIS AND CHARACTERIZATION OF A NOVEL REACTIVE LADDER-LIKE POLYSILSESQUIOXANE WITH LATERAL BROMOPHENYL GROUPS

A novel soluble, reactive ladder-like polysilsesquioxane (L) with lateral bromophenyl groups was synthesized successfully by stepwise coupling polymerization (SCP) method including preaminolysis, hydrolysis and polycondensation steps. The monomer 3-trichlorosilylpropoxy-4-bromobenzene (M) was first prepared via hydrosilylation reaction catalyzed by dicyclopentadienylplatinum dichloride (Cp2PtCl2). The title polymer (L) was characterized by 1H-NMR, 29Si-NMR, FTIR, X-ray diffraction (XRD), DSC and vapor pressure osmometry (VPO). The experimental results indicate that the polymer (L) possesses a typical ladder-like structure.

Synthesis and Crystal Structure of a Novel Double Ladder-like Cadmium Coordination Polymer Constructed by Isophthalate and Imidazole

A novel double ladder-like cadmium coordination polymer with isophthalate as bridging ligands and imidazole as non-chelating capping ligands was obtained by a hydrothermal reaction. The crystal is of triclinic, space group P1 with a = 10.266（2）, b = 11.573（2）, c = 12.651（3） A, α = 89.95（3）, β= 74.64（3）,γ = 68.54（3）°, C50H44Cd4N12O18, Mr = 1550.57, V= 1341.1（5）A3, Dc = 1.920 g/cm3, F（000） = 764, p = 1.651 cm-1 and Z = 1. The final refinement gave R = 0.0342 and wR = 0.0738 for 5925 observed reflections with I 〉 2σ（I）. The crystal structure involves two different ladder-like chains, which are further connected to form a ladder-like double chain architecture via hydrogen bonding interactions, extending along the b axis. The weak interactions including hydrogen bonds and π-π staking interactions contribute to the alignment of the complex in the crystalline state.

Crumbs homolog 2 mutation in two siblings with steroid-resistant nephrotic syndrome:Two case reports

BACKGROUND Crumbs homolog 2(CRB2)is a recently discovered gene that is closely related to the maintenance of normal polarity in podocytes;mutations can directly lead to steroid-resistant nephrotic syndrome(SRNS).However,the characteristics of nephrotic syndrome(NS)caused by CRB2 mutations have not been described.CASE SUMMARY We report a novel compound heterozygous mutation of the CRB2 gene in two siblings with SRNS.The two siblings had edema,proteinuria,hypoproteinemia and hyperlipidemia.Both their father and mother had normal phenotypes(no history of NS).Whole exon sequencing(WES)of the family showed a novel compound heterozygous mutation,c.2290(exon 8)C>T and c.3613(exon 12)G>A.Glucocorticoid therapy(methylprednisolone pulse therapy or oral prednisone)and immunosuppressive agents(tacrolimus)had no effect.During a 3-year follow-up after genetic diagnosis by WES,proteinuria persisted,but the patient was healthy.CONCLUSION CRB2 mutations related to SRNS often occur in exons 7,10,and 12.Clinical manifestations of SRNS caused by CRB2 mutations are often less severe than in other forms of SRNS.

Synthesis and Structure of a Ladder-like Co-crystal Cu^ICl with 3,5-Dipropyl-4-amino-1,2,4-triazole

Redox reaction of a mixture of CuC12·2H2O, HaPO3 and dpatrz （3,5-dipropyl- 4-amino-1,2,4-triazole） at room temperature yields one new compound, [Cu（1）（/.t2-dpatrz）aCu（1）CI2], with two independent cis- and trans-propyl side chain molecules. Compound 1 crystallizes in monoclinic, space group P21/c with a = 7.474（1）, b = 17.807（1）, c = 18.851（1） A, β = 108.32（1）°, V = 2381.7（2） A3, Z = 4, C16H32CI2Cu2Ns, Mr = 534.48, Dc = 1.491 g.cm-3,μ = 2.03 mm-1, F（000） = 1104, GOOF = 1.050, the final R = 0.0445 and wR = 0.1162 for 3162 observed reflections （I 〉 2σ（I））. Compound 1 shows discrete dimeric structures （A and B） containing inversion centers and the Cu（l） ions are coordinated in triangle geometries. The isomers are connected by N-H…CI hydrogen bonds, chains with graph-set C（7） and rings R22（14） and C-H..＇Jr interactions into stair-step chains （Tapes A and B） running parallel to the [01-1] direction. The N-H…C1 hydrogen bonds result in chain and cyclic structures with graph-sets C22（17） and R34（18） linking tapes A and B to form two-dimensional networks along the [031] direction. Packing of crystal 1 is stabilized by rings R34（18） and weak C-H…C1 hydrogen bonds parallel to the [01-2] direction. Bond valence sum （BVS） and UV-Vis absorption spectra support the existence of Cu（I） ions. Compound 1 exhibits extensive green blue phosphorescence in the solid state at room temperature.

SYNTHESIS AND CHARACERIZATION OF LADDER-LIKE POLYDECYLSILSESQUIOXANE

Ladder-like polydecylsilsesquioxane (LPDS) was synthesized by a preamminolysis reaction ofdecyltrichlorosilane with ethylene diamine, followed by hydrolysis and polycondensation reaction. LPDS issoluble in a nonpolar solvent such as heptane and insoluble in a high polar solvent such as methanol. It wascharaterized by IR, ~1H-NMR, ^(29)Si-NMR, GPC and X-ray diffraction.

Synthesis and characterization of a ladder-like structure compound formed by cadmium (II) and anionic nitronyl nitroxide

A ladder-like structure compound formed by cadmium (II) and anionic nitronyl nitroxide, [Cd2(NITpBA)4(H2O)4] (where NITpBA = 2-(4-carboxyphenyl)4,4,5,5-tetramethyl-4,5-dihydro-imidazol-1-oxyl-3-oxide), has been synthesized and characterized. X-ray analysis reveals that the compound 1 crystallizes in the monoclinic P21/c (No. 14) space group, and [Cd2(NITpBA)4(H2O)4] units are linked into infinite chains by radical bridging ligands.

Dynamic Behaviour of Pond Ash Mixed with Coir and Crumb Rubber

An experimental program was conducted to determine the dynamic properties of pond ash(PA)and pond ash mixed with admixtures like crumb rubber(CR)and coir fiber(CF)which are wastes of different industries.Additives CR and CF were mixed separately with pond ash in a percentage ranging from 0%–20%at an interval of 5%and 0%–2%at an interval of 0.5%by weight of pond ash,respectively.Dynamic properties(Shear modulus,Number of cycles to initiate liquefaction,Degradation index and Damping ratio)for pond ash mixes were determined using strain-controlled cyclic tri-axial apparatus.The shear strain was kept constant at 1.2%and the frequency varied from 0.4 to 1 Hz.Liquefaction resistance improves when crumb rubber is added to pond ash,however adding Coir fiber to pond ash has essentially no effect on the number of cycles required to initiate liquefaction.The dynamic shear modulus decreases when crumb rubber was mixed in pond ash whereas when coir fiber is added to pond ash,the dynamic shear modulus increases significantly.A mix of PA,CR,and CF was also tried to balance the number of cycles required to initiate liquefaction and shear modulus.The mixed sample in the proportion of PA(84%)+CR(15)+CF(1%)can be termed a balanced mix as the number of cycles to initiate liquefaction was more than all PA-CF mixes and also the shear modulus was more than all PA-CR mixes.Damping ratio increases with increasing frequency of loading(0.4 to 1 Hz).

Using Crumb Rubber to Improve the Properties of Concrete from the Sustainability Point of View

In the past decade,the economy of China has been increasing at a remarkable speed,which causes the rapid increase of automobile industry.Researches in past years demonstrated that the incorporation of crumb rubber into concrete reduces the mechanical strength of concrete.However,properties such as plastic shrinkage cracking resistance,dry shrinkage cracking resistance,frost resistance,impact resistance,fatigue resistance etc.are improved due to the deformable feature of crumb rubber.Therefore,it is expected that using crumb rubber in concrete not only improves the properties of the material,but also reduces the environmental pressure of handling waste tires.

基于楔入劈拉试验的橡胶混凝土软化本构模型

为确定橡胶混凝土的软化本构模型,对2种缝高比和5种橡胶掺量的混凝土试件进行楔入劈拉试验研究和理论分析。依据试验结果,采用规范公式及能量守恒定律计算混凝土试件的断裂韧度和断裂能,并求解出PETERSSON、欧洲混凝土规范、XU和REINHARDT3种软化曲线的相关参数。根据3种软化本构关系得到黏聚韧度理论值,并与黏聚韧度试验计算值进行了对比分析。研究结果表明:掺入适量的橡胶集料可提高混凝土的断裂失稳韧度和断裂能;3种典型的混凝土软化模型均不能很好地描述橡胶混凝土的黏聚韧度;对XU和REINHARDT软化关系式进行修正后,得到了橡胶混凝土的软化本构模型,可用于研究橡胶混凝土材料的抗裂特性。

变温条件下胶粉改性沥青混凝土抗裂特性研究

为探究胶粉改性沥青混凝土在水利工程环境下的抗裂性能,本文基于小梁弯曲试验及全应力-应变曲线脆性指标,探究了变温条件下胶粉改性沥青混凝土的抗裂性能。结果表明:胶粉改性沥青抗裂性能最优时的配比为80M,掺量20%;低温条件下,胶粉改性沥青混凝土的抗弯强度及峰值应变优于基质沥青混凝土;温度越低,沥青混凝土的脆性指标越大,试件越容易开裂;胶粉的加入降低了基质沥青混凝土的脆性指标,使其抗裂性能有所提升,同时改变了基质沥青混凝土在-10℃时的受力状态,其应力-应变曲线由脆性特征转变为塑性特征。本文研究有望为面板选材提供新思路。

硫化橡胶沥青和脱硫橡胶沥青的性能评估

文中采用扫描电子显微镜(SEM)观察了硫化橡胶粉和脱硫橡胶粉的微观形貌.以硫化橡胶粉和脱硫橡胶粉作为改性剂制备了改性沥青,并以基质沥青作为对照组,评估了三种沥青的高温性能和低温抗裂性能.通过红外光谱分析了其改性机理.结果表明:硫化胶粉颗粒表面光滑,棱角明显,颗粒相对分散.脱硫胶粉颗粒表面粗糙,颗粒有团聚现象.硫化胶粉改性沥青的粘度最大,脱硫胶粉改性沥青粘度次之,基质沥青的粘度最小.胶粉脱硫可以降低沥青的温度敏感度.硫化胶粉的高温抗车辙性能和低温抗裂性能均优于脱硫胶粉改性沥青.硫化胶粉改性沥青和脱硫胶粉改性沥青其主要以物理混溶为主.

Performance Characterization of CR/PU Asphalt for Potential Application in Assembled Fast-Repairing Engineering

Conventional repairing methods for asphalt pavement have some inconveniences,such as insufficient strength,and are typically time-consuming.To address these issues,this study proposes a new technological method to design and prepare a high-performance assembled asphalt concrete block for fast repair of the potholes.A series of composite modified asphalt binders with 10%crumb rubber(CR)and different dosages(0%,1%,3%,5%)of polyurethane(PU)are examined to determine the optimized binder.Subsequently,the corresponding asphalt mixtures are prepared for further comparison and assessment of engineering properties,such as moistureinduced damage,high-temperature deformation,and low-temperature cracking characteristics.The test results show that PU can significantly improve the high-temperature performance and hardness of(crumb rubber modified asphalt)CRMA binder;3%PU contributes allowing the resistance of CRMA mixture to moisture-induced damage at higher levels,particularly under water whole immersion;as 3%PU is added,the high-temperature rutting deformation resistance of the CRMA mixture increases significantly,and the low-temperature anti-cracking properties are also improved slightly.Therefore,the innovatively designed high-quality assembled fast-repairing asphalt concrete block is recommended as an appropriate option for highway maintenance.

沙光鱼片裹浆工艺优化

为优化沙光鱼片的加工工艺,增加沙光鱼片裹浆后的口感,该试验以沙光鱼片为原料,水、淀粉、面粉、食盐为辅料,制备裹浆,在单因素试验的基础上,采用响应面法对沙光鱼片裹浆的制备工艺进行优化。结果表明,裹浆的最佳工艺条件为固定5 cm×3 cm×2 cm规格的沙光鱼片6 g、30%(质量分数以原料鱼片计)水、13%木薯淀粉、12%面包粉、1%食盐,固定料油体积比1∶3、油炸温度190℃、油炸时间1.5 min,复炸时间30 s时沙光鱼片的感官评分最高(89.52分),口感最佳。

生物油和活化胶粉共混改性老化沥青

为改善再生沥青路面(RAP)的再生混合料性能,将不同掺量微波活化胶粉(ACR)和废食用油(WCO)加入到老化沥青中,对其进行流变性能、微观特性分析。结果表明:WCO不仅可以作为再生剂改善老化沥青,而且还可以作为增溶剂充分溶胀胶粉提升沥青的综合性能;荧光显微镜(FM)分析表明,适量WCO有助于提升胶粉在沥青中的相容性。傅立叶红外光谱(FTIR)结果表明,老化沥青再生过程为物理共混,而胶粉改性过程伴随着少量的化学反应。最后,结合改性再生沥青的流变结果与微观结构,建议WCO和ACR的最佳用量为10%和18%。

废旧橡胶粉对水泥稳定碎石基层力学及干缩性能的影响

为研究废旧橡胶粉对水泥稳定碎石基层混合料力学及干缩性能的影响,将不同粒径和掺量的橡胶粉以等质量等粒径的方式替换集料,通过考察劈裂强度、弯拉强度、抗压回弹模量和干缩试验研究了不同粒径和掺量的橡胶粉对水泥稳定碎石基层混合料力学及干缩性能的影响。结果表明,掺入橡胶粉降低了水泥稳定碎石基层混合料的劈裂强度、弯拉强度和抗压回弹模量,抑制了水泥稳定碎石的干燥收缩,且橡胶粉的粒径和掺量越大性能变化幅度越大。相较于不掺橡胶粉,掺入40目质量分数0.50%的橡胶粉时,水泥稳定碎石混合料90 d的劈裂强度、弯拉强度和抗压回弹模量分别降低了32.8%、51.0%和26.3%,累计干缩应变和干缩系数分别降低了27.4%和25.5%。

胶粉改性沥青在高速公路路面养护中的应用研究

以京张高速公路养护实况为例,针对胶粉改性沥青在高速公路路面养护中的应用进行探讨,介绍了胶粉改性沥青中沥青、集料、矿料级配掺配的各项指标要求,分析了最佳油石比的确定方法,对制备完成的胶粉改性沥青混合料进行高温稳定性和低温抗裂性检验,结果均满足相关规范的要求。

胶粉复合改性沥青的研制及工业化生产

以某公司加工的环烷基和中间基原油为原料,通过优化原油配比,采用常减压蒸馏工艺,通过控制减压蒸馏工艺条件,生产出符合技术标准要求且能够用于改性沥青的基质沥青原料。通过筛选与基质沥青配伍性好的胶粉、助剂添加比例及工艺条件优化,研制出符合技术规范要求的胶粉复合改性沥青并进行了工业试生产。对工业生产的产品检测及专业部门鉴定结果表明,胶粉复合改性沥青产品各项技术指标符合JT/T798-2019《路用废胎胶粉橡胶沥青》技术要求,成功实现了工业化生产和应用。