This research was to clone, express, and analyze the structure and function of major molecules of porcine interleukin family. Genes of porcine interleukin family were cloned by RT-PCR from stimulated porcine PBMC by L...This research was to clone, express, and analyze the structure and function of major molecules of porcine interleukin family. Genes of porcine interleukin family were cloned by RT-PCR from stimulated porcine PBMC by LPS and PHA, and then expressed in E. coli, and the structure and function of these molecules were predicted by ExPASY. The results showed that genes of IL-4, IL-6, and IL-18 were successfully cloned and expressed. Furthermore, the expression products of recombinant IL-4 and IL-6 both have multiple biological activities. By analyzing these genes with the NCBI/GenBank data, the homologies of the nucleotide acid sequence are 99.25, 99.21, and 100%, respectively, and have great species differences when compared with other animal species. The results of the prediction showed that all these molecules contain several phosphorylation, glycosylation, protein kinase, and signal transduction bonding sites in secondary structure, and all are compact globularity protein in space configuration. These characteristics of structure are the basis for their multiple biological functions. The genes, structure and function of key molecular of porcine interleukin family were successfully cloned, expressed, and analyzed in this paper.展开更多
This paper analyzes the family factor in the construction of new socialist countryside. It is believed that the family plays both the positive role and negative role in new socialist countryside construction. Based on...This paper analyzes the family factor in the construction of new socialist countryside. It is believed that the family plays both the positive role and negative role in new socialist countryside construction. Based on this analysis,it puts forward corresponding countermeasures,including bringing into play the effect of family in promoting production and carrying forward excellent factors of family culture.展开更多
This article aims to provide an adequate interpretation for the dynamic construction of identity in private setting context in China. Naturally occurring conversations between single mother and daughter were examined ...This article aims to provide an adequate interpretation for the dynamic construction of identity in private setting context in China. Naturally occurring conversations between single mother and daughter were examined by using discourse analysis. The result shows that participants in spoken interaction do not always communicate with each other through the expected/default identities. Rather they sometimes deviate from the identities that they are expected to construct.展开更多
Without beautiful countryside,there will be no beautiful China.The family farm is a beautiful scene in the construction of new socialist countryside.However,the construction of family farms just starts in China.It sti...Without beautiful countryside,there will be no beautiful China.The family farm is a beautiful scene in the construction of new socialist countryside.However,the construction of family farms just starts in China.It still remains at pilot and fumbling stage.There are still mainly unfavorable factors including separate land,insufficient funds,lack of security for farmers,lack of talents,and service lagging behind.On the basis of analyzing various weak points,we put forward countermeasures and recommendations for construction of family farms on promoting orderly farmland circulation,strengthening financial support,improving professional quality,supplying social security,increasing public investment,and attaching importance to standardized operation.展开更多
Family education is brought to the attention since ancient times ,good family trait is through the ages many dignitaries of cultivate our morality and set up home." Benevolence " thought is the core of Confucianism,...Family education is brought to the attention since ancient times ,good family trait is through the ages many dignitaries of cultivate our morality and set up home." Benevolence " thought is the core of Confucianism, and is also the important content of Chinese excellent traditional culture. In this paper, the content of the Confucian" benevolence "thought and its fit with modem start-up construction has carried on the preliminary analysis, probes into the Confucian idea of" love" to restore the meaning of good family trait展开更多
文摘This research was to clone, express, and analyze the structure and function of major molecules of porcine interleukin family. Genes of porcine interleukin family were cloned by RT-PCR from stimulated porcine PBMC by LPS and PHA, and then expressed in E. coli, and the structure and function of these molecules were predicted by ExPASY. The results showed that genes of IL-4, IL-6, and IL-18 were successfully cloned and expressed. Furthermore, the expression products of recombinant IL-4 and IL-6 both have multiple biological activities. By analyzing these genes with the NCBI/GenBank data, the homologies of the nucleotide acid sequence are 99.25, 99.21, and 100%, respectively, and have great species differences when compared with other animal species. The results of the prediction showed that all these molecules contain several phosphorylation, glycosylation, protein kinase, and signal transduction bonding sites in secondary structure, and all are compact globularity protein in space configuration. These characteristics of structure are the basis for their multiple biological functions. The genes, structure and function of key molecular of porcine interleukin family were successfully cloned, expressed, and analyzed in this paper.
文摘This paper analyzes the family factor in the construction of new socialist countryside. It is believed that the family plays both the positive role and negative role in new socialist countryside construction. Based on this analysis,it puts forward corresponding countermeasures,including bringing into play the effect of family in promoting production and carrying forward excellent factors of family culture.
文摘This article aims to provide an adequate interpretation for the dynamic construction of identity in private setting context in China. Naturally occurring conversations between single mother and daughter were examined by using discourse analysis. The result shows that participants in spoken interaction do not always communicate with each other through the expected/default identities. Rather they sometimes deviate from the identities that they are expected to construct.
文摘Without beautiful countryside,there will be no beautiful China.The family farm is a beautiful scene in the construction of new socialist countryside.However,the construction of family farms just starts in China.It still remains at pilot and fumbling stage.There are still mainly unfavorable factors including separate land,insufficient funds,lack of security for farmers,lack of talents,and service lagging behind.On the basis of analyzing various weak points,we put forward countermeasures and recommendations for construction of family farms on promoting orderly farmland circulation,strengthening financial support,improving professional quality,supplying social security,increasing public investment,and attaching importance to standardized operation.
文摘Family education is brought to the attention since ancient times ,good family trait is through the ages many dignitaries of cultivate our morality and set up home." Benevolence " thought is the core of Confucianism, and is also the important content of Chinese excellent traditional culture. In this paper, the content of the Confucian" benevolence "thought and its fit with modem start-up construction has carried on the preliminary analysis, probes into the Confucian idea of" love" to restore the meaning of good family trait